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Energetic-protein supplementation in the last 60 days of gestation improves performance of beef cows grazing tropical pastures

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Journal of Animal Science and Biotechnology 2018年第1期9卷 208-216页

作者： Aline Gomes da silva mário Fonseca Paulino Edenio Detmann Henrique Jorge Fernandes Lincoln da silva Amorim Román Enrique maza Ortega Victor Valério de Carvalho Josilaine Aparecida da Costa Lima Felipe Henrique de moura mariana Benevides monteiro Jéssika Almeida Bitencourt Universidade Federal de Vicosa VicosaMinas Gerais 36570-000Brazil Universidade Federal de mato Grosso do Sul Campo GrandeMato Grosso do Sul 79074-460Brazil Universidade Estadual de mato Grosso do Sul AquidauanaMato Grosso do Sul 79200-000Brazil Biotran Biotecnologia e Treinamento em Reproducao Animal AlfenasMinas Gerais 37130-000Brazil Universidade Federal do Acre Rio BrancoAcre 69920-900Brazil

Background： Nutrition is one of the most important factors that affect animal performance, and it therefore also impacts on financial results in beef systems. In this way, finding the best strategy for feeding supplements is of paramount importance. Aiming to evaluate the effect of supplement feeding strategies for beef cows in the last third of gestation, two experiments were conducted. In Experiment 1, 35 pregnant Nellore cows were assigned to a completely randomized design with four treatments： control, which received no supplement; supplementation for the last 30 d of gestation（30-d; 3.0 kg/d）; supplementation for the last 60 d of gestation（60-d; 1.5 kg/d）; or supplementation for the last 90 d of gestation（90-d; 1.0 kg/d）. All supplemented treatments received the same total amount of supplement throughout the experiment： 90 kg（20% of crude protein）. A second experiment（Experiment 2） was delineated to evaluate the effects of the amounts offered in Experiment 1 on intake and metabolism. Four multiparous pregnant Nellore cows were assigned to a 4 × 4 Latin square design, with periods of15 d ***： There was a linear effect of the number of days of supplementation on calving body weight（BW; P 〈 0.05）and a quadratic effect on BW change from parturition to d 31 post-calving（P 〈 0.05）, with cows on the 60-d strategy losing less BW post-calving. No difference was found in offspring birth BW（P 〉 0.10）. A significant linear effect on interval from parturition to conception（P 〈 0.05） was observed, with the highest calving to conception interval being observed in the 90-d strategy. The level of supplementation did not affect forage intake or neutral detergent fiber digestibility（P 〉 0.10）. Nitrogen excreted through urine tended to increase linearly with the level of supplementation（P 〈 0.10）.Conclusion： Providing 1.5 kg of supplement during the last 60 d of gestation improves cow performance after calving, reducing the magnitude of BW lost, and reduces the number of

关键词： Flushing Nutrition Parturition Reproduction

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Ecotoxicological Tools for Landfarming Soil Evaluation in a Petrochemical Complex Area

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Pedosphere 2014年第2期24卷 280-284页

作者： F.m.R.da silva JUNIOR P.F.silva F.S.GUImARAES K.A.DE ALmEIda P.R.m.BAISCH A.L.mUCCILLO-BAISCH Laboratório de Ensaios Farmacológicos e Toxicológicos Instituto de Ciências BiológicasUniversidade Federal do Rio Grande do Sul (FURG) Laboratório de Oceanografia Geológica Instituto de OceanografiaUniversidade Federal do Rio Grande do Sul (FURG) Programa de Pós graduao em Ciências Fisiológicas Fisiologia Animal ComparadaInstituto de Ciências BiológicasUniversidade Federal do Rio Grande do Sul (FURG)

The demand for simple and rapid bioassays in ecotoxicological evaluations is of paramount importance in order to speed up environmental monitoring *** this study we performed bioassays with lettuce seeds and two species of terrestrial isopods (Armadillidium vulgare and Porcellio dilatatus) for the ecotoxicological assessment of a landfarming soil from a petrochemical complex *** solubilized content of test soil demonstrated a concentration-response type toxic effect on seed germination rate,and a delay on germination,but showed toxic effect on seedlings wet weight only at the highest *** effects were also observed in mortality rate and avoidance behavior of the two woodlice *** results demonstrated the sensitiveness of the organisms studied,and highlighted the possibility to use these bioassays in environmental monitoring programs in areas contaminated with fossil fuels.

关键词： Armadillidium vulgare lettuce Porcellio dilatatus terrestrial woodlice

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Treatment of Vulvovaginal Candidiasis with A Strategy Based on Phototherapy (m.A.C.^® Scar Acceleration method)

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modern Research in Inflammation 2024年第1期13卷 1-7页

作者： marcus Vinicius de mello Pinto Karin Yuri Fernández Iturra Aline Ronis Sampaio maría Elena silva Álvarez Esteban Fortuny Celulare Institute Petrópolis Brazil Bienus Clinic Santiago Chile Fisiocare Curicó Chile Universidad Finis Terrae Santiago Chile

Candidiasis, also known as candidiasis vulvovaginitis, is an infection caused by different types of Candida fungi, the most frequent being Candida albicans. The present study reports an effective strategy, which opens new avenues for the treatment of this public health problem. The mAC® methodology, conventional laser light-emitting (LLLT)/LED) methods are based on the biphasic response demonstrated many times in LLLT research and as with other forms of drugs, a “drug” (irradiation parameters) and a “dose” (irradiation times) and the “Arndt-Schulz Law” is often cited as a suitable model to describe the dose-dependent effects of LLLT. This method uses photopharmaceuticals, cell markers and the use of correct parameters for each case to induce the acceleration of tissue repair. The present study shows a case of a 32-year-old patient diagnosed with recurrent candidiasis 4 years ago. Eighteen sessions were performed (every other day) using a photoactivated component (methylene blue 1% + Clotrimazole 1%) and LED phototherapy (red, blue and violet) with emission times of 60 - 260 seconds for each applicator, according to the dose recommendations of the scar acceleration method (mAC®). At the sixth treatment session there was a noticeable decrease in the itching sensation reported by the patient. In session 11 she reported feeling a great improvement, indicating that she no longer felt itching in any area after 18 sessions. The present case demonstrates new methodologies to treat common problems in the population that have a positive impact on the quality of life. This methodology has a promising future because it is non-invasive and requires a great biological transformation for inflammatory, fungal and viral control.

关键词： Treatment of vulvovaginal Candidiasis Candidiasis Candida m.A.C. mAC®

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Effectiveness of different biochars in remediating a salt-affected Luvisol in Northeast Brazil

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Biochar 2021年第2期3卷 149-159页

作者： Wallace melo dos Santos maria Isidoria silva Gonzaga JoséAiron da silva AndréQuintão de Almeida JoséCarlos de Jesus Santos Thomas Antonio silva Gonzaga Idamar da silva Lima Eloámoura Araújo Water Resources Graduate Program Federal University of SergipeSão CristóvãoSE 49100‑000Brazil Agronomy Department Federal University of SergipeSão CristóvãoSE 49100‑000Brazil Agriculture Engineering Department Federal University of SergipeSão CristóvãoSE 49100‑000Brazil University of Notre dame Notre DameIN 46556USA Soils and Agricultural Engineering Department Federal University of ParanáCuritibaPR 80060‑000Brazil

Biochar has been proposed as a soil amendment to reclaim salt-affected ***,the evaluation of the different biochar types in these soils is essential due to the significant variation among biochar *** this study,biochars from sugarcane bagasse(SB),orange bagasse(OB),and corncob(CB)were applied alone and in the presence of gypsum(G)to test their potential to remediate a highly degraded saline-sodic soil in Brazil´s Northeast *** that,we conducted a laboratory column-leaching experiment and a greenhouse *** the column-leaching test,we analyzed the sodium adsorption ratio(SAR),the electrical conductivity of the saturation extract(ECe),the concentrations of major ions,and the exchangeable sodium percentage(ESP).maize plants were grown in the leached soil in a ***-and corncob-derived biochars were more efficient than gypsum in reducing the soil salinity,bringing the indicators below threshold values,and optimizing soil reclamation *** ECe,SAR,and ESP were reduced to 3.42 dS m−1,1.64(mmolc dm−3)0.5 and 4.86%,respectively,in the SB treatment;and to 3.19 dS m−1,0.88(mmolc dm−3)0.5 and 2.53%,respectively,in the CB *** bagasse biochar did not effectively reduce the salinity *** biochar treatments improved seed germination and plant *** SB,CB,SBG,and CBG treatments increased plant height(478–558%),shoot mass(783–983%),and root mass(386–500%),***,the application of SB and CB is an effective alternative to remediate saline-sodic soils and to reduce the impact of soil salinity in the environment.

关键词： Biochar Soil remediation Soil salinity Corn growth

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How do greenhouse gas emissions vary with biofertilizer type and soil temperature and moisture in a tropical grassland?

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Pedosphere 2020年第5期30卷 607-617页

作者： Abmael da silva CARDOSO Juliana Bega JUNQUEIRA Ricardo Andrade REIS Ana Claudia RUGGIERIAnimal Science Departament College of Agricultural and Veterinarian SciencesSao Paulo State UniversityJaboticabal 14884-900(Brazil) Kenji Agricultural and Environmental Consultancy Ribeirdo Preto 14010-079(Brazil)

Greenhouse gases are known to play an important role in global *** this study,we determined the effects of selected soil and climate variables on nitrous oxide(N2O),methane(CH4),and carbon dioxide(CO2)emissions from a tropical grassland fertilized with chicken slurry,swine slurry,cattle slurry,and cattle *** N2O emissions did not differ between treatments and varied from 29.26 to 32.85 mg N m^-***,cumulative CH4 emissions were not significantly different among the treatments and ranged from 6.34 to 57.73 mg CH4 m^-*** and compost application induced CO2 emissions that were significantly different from those in the control *** CH4 conversion factors measured were 0.21%,1.39%,4.39%,and 5.07%for cattle compost,chicken slurry,swine slurry,and cattle slurry,respectively,differing from the recommendations of the Intergovernmental Panel on Climate Change(IPCC).The fraction of added Nemitted as N2O was 0.39%,which was lower than the IPCC default value of 2%.Our findings suggest that N2O emissions could be mitigated by replacing synthetic fertilizer sources with either biofertilizer or *** results indicate the following:N2O emission was mainly controlled by soil temperature,followed by soil moisture and then soil NH^"content;CH4 fluxes were mainly controlled by soil moisture and chamber headspace temperature;and CO2 fluxes were mainly controlled by chamber headspace temperature and soil moisture.

关键词： emission factor methane conversion factor methane oxidation methane production capacity slurry fertilizer soil moisture

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Serological evidence of Toxoplasma gondii infection as potential risk for the development of lepromatous leprosy in an endemic area for both neglected tropical diseases in Brazil

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Infectious Diseases of Poverty 2020年第1期9卷 36-45页

作者： Luciana Regina Pereira Oliveira Lívia mattos martins Rebeka da Conceição Souza Yuri Scheidegger de Castro Letícia silva Nascimento Juliana Azevedo da silva Edilbert Pellegrini Nahn Junior Wilmar Dias da silva Alba Lucínia Peixoto-Rangel Laboratory of Recognition Biology Center of Biosciences and BiotechnologyState University of Northern Rio de JaneiroCampos dos GoytacazesRJBrazil Campos medical School Campos dos GoytacazesRJBrazil Laboratory of Immunochemistry Butantan InstituteSão PauloSPBrazil

Background:mycobacterium leprae and Toxoplasma gondii infections are both neglected tropical diseases highly prevalent in *** with certain parasite species can significantly alter susceptibility to other important pathogens,and/or influence the development of *** we investigated the possible influence of ***/*** co-parasitism on the manifestation of leprosy and its clinical ***:Participants(n=291)were recruited in Campos dos Goytacazes city,Rio de Janeiro state,southeast Brazil,from August 2015 to December 2019 and clinically diagnosed for *** were selected based on the presence(patients)or absence(healthy controls)of the leprosy *** of patients were also recruited for this *** samples from patients(n=199)with leprosy,contacts(n=40)and healthy controls(n=52)were investigated for levels of Igm and IgG anti-phenolic glycolipid-1(PGL-1)by ***,IgG antibody against soluble Toxoplasma antigen(STAg)was measured in sera samples from leprosy patients,contacts and healthy controls for Toxoplasma gondii serology by ***-PGL-1 IgG and Igm levels were compared using one-way ANOVA Kruskal-Wallis or mann-Whitney,while Spearman test was used to correlate levels of IgG anti-STAg and Igm/IgG anti-PGL-1 from seropositive and seronegative individuals for *** *** risk of *** infection for leprosy disease was assessed using Fisher’s ***:Levels of Igm anti-PGL-1 antibodies were significantly higher in multibacillary(mB)patients compared to paucibacillary(PB)patients(P=0.0068).Higher Igm and IgG levels anti-PGL-1 were detected in patients with the lepromatous *** serologic prevalence for *** infection was 74.9%.We detected increased anti-STAg antibody levels in leprosy patients(79.4%),reaching 88.8%within those with lepromatous form of this *** leprosy risk increase in *** seropositive individuals was two-fold(odds ratio[OR]=2.055;95% c

关键词： Leprosy Toxoplasmosis Coinfection Co-immunomodulation Immunoglobulins Immune response

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Efficiency of soil-applied ^(67)Zn-enriched fertiliser across three consecutive crops

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Pedosphere 2021年第4期31卷 531-537页

作者： Edson m.mATTIELLO Eduardo L.CANCELLIER Rodrigo C.da silva Fien DEGRYSE Roslyn BAIRD mike J.mCLAUGHLIN Department of Soil Science Universidade Federal de ViçosaViçosaMinas Gerais(Brazil) Department of Soil Science Universidade Federal de LavrasMinas Gerais(Brazil) The University of Adelaide School of AgricultureFood and WineFertiliser Technology Research CentrePMB 1Waite CampusGlen Osmond SA 5064(Australia)

A very small amount of applied zinc(Zn) is taken up by crops, resulting in low recovery by plants. Adding elemental sulphur to zinc oxide(ZnO) fertiliser could improve Zn solubilisation and exert a higher residual effect on crops than soluble Zn sources. We produced an isotopically labelled Zn-elemental sulphur fertiliser and evaluated its performance in comparison to traditional Zn sources during sequential crop cultivation. Three ^(67)Zn-labelled fertilisers,ZnO, zinc sulphate(ZnSO_(4)), and ZnO co-granulated with elemental sulphur(ZnOS^(0)), were soil applied, and their contributions to the uptake of Zn by three consecutive crops, wheat, ryegrass, and corn, were assessed in a 294-d pot experiment. The contributions of Zn fertilisers followed the order: ZnSO_(4) > ZnO= ZnOS^(0). The relative contributions of Zn fertilisers were lower in the first crop than in the subsequent crops. The overall recovery of applied Zn by the three crops was higher for ZnSO_(4) than for ZnO and ZnOS^(0), reaching 1.56%, 0.45%, and 0.33% of the applied Zn, respectively. Zinc recovery by plants was very low, regardless of the source of Zn. Adding elemental sulphur to ZnO did not increase its effectiveness up to 294 d after application. Fertiliser contribution was higher for the subsequent crops than for the initial crop, indicating the importance of assessing the residual effects of Zn fertilisers.

关键词： agronomic biofortification elemental sulphur fertilizer residual effect plant uptake Zn bioavailability Zn fertilization Zn source

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Effects of aeration on the suspended matter from a tropical and eutrophic estuary

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Journal of Environmental Sciences 2019年第12期31卷 175-186页

作者： Natascha Krepsky maria das Gracas S.Bispo Luiz F.Fontana Frederico S.da silva Jodo P.m.Torres Olaf malm Cleverson G.silva mirian A.C.Crapez Laboratory of marine Geology Fluminense Federal UniuersityAue.Gen.Milton Tauares de Souza s/n°-Gragoata24210-346 NiterdiRJBrazil Radioisotopes Laboratory Eduardo Penna Franca Biophysics Institute Carlos Chagas FilhoFederal University of Rio de JaneiroAu.Carlos Chagas Filho373 CCS-Bl.G21941-902 Rio de JaneiroRJBrazil marine Biology Department Fluminense Federal UniversityR.Mario Santos Bragas/nNiteroi24.020-141 NiteroiRJBrazil

A comprehensive understanding of the complex biogeochemical interactions between organic matter and persistent contaminants in the suspended matter is vital for ecoefficient estuary ***,little is known regarding aeration effects in suspended particulate ***,this study aimed to investigate the effects of aeration on the suspended matter from a Tropical and Eutrophic estuarine *** water with 60 g/L of suspended particulate matter(SPm)was collected from Guanabara Bay,Rio de Janeiro,Brazil,transferred to experimental boxes and aerated for 61 *** aggregates monitoring included abiotic variables measurements and,determination of total organic matter(TOm),biopolymers composition,bacterial activity,trace metals,and polycyclic aromatic hydrocarbons(PAHs)*** aeration enhanced dissolved oxygen(DO)concentration and the redox potential(Eh).However,from days 0 to 61 the predominant bacterial activities were denitrification and *** transport system activity increased after day 10,and aerobic activity was detected after day *** summary,aeration increased aerobic bacterial activity,lipids(LIP)and trace metal concentrations,although diminished protein/carbohydrate ratio and PAH *** metals concentration(Ni,Pb,Cu,Cr,mn,and Fe)were the highest on day 19 when the p H was *** presented toxic values(Cu>20.0μg/g).The p H showed a strong negative correlation with Eh(r=-0.94;pment(p H≤5.9)in marine ecosystems with high loads of toxic trace metals is unsafe for ***,managers must be aware of the environmental and biological risks of introducing the aeration technique into a eutrophic marine environment.

关键词： mixed-pollution Remediation Acidification Esterases enzymes Biopolymer

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An empiric equation for the latent heat of vaporization of moisture in bananas during its isothermal drying

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Agricultural Sciences 2012年第2期3卷 214-220页

作者： Wilton Pereira da silva Cleide maria Diniz Pereira da silva e silva fernando José de Almeida Gama Josivanda Palmeira Gomes Federal University of Campina Grande Campina Grande Brazil

In order to determine the energy needed to artificially dry a product, the latent heat of vaporization of moisture in the product, hfg, must be known. Generally, the expressions for hfg reported in the literature are of the form hfg = h(T)f(m), where h(T) is the latent heat of vaporization of free water, and f(m) is a function of the equilibrium moisture content, m. But expressions of this type contain a simplification because, in this case, the ratio hfg/h would only depend to the moisture content. In this article a more general expression for the latent heat of vaporization, namely hfg = g(m,T), is used to determine hfg for banana. To this end, a computer program was developed which fits automatically about 500 functions, with one or two independent variables, imbedded in its library to experimental data. The program uses nonlinear regression, and classifies the best functions according to the least reduced chi-square. A set of executed statistical tests shows that the generalized expression used in this work given by hfg = g(m,T) produces better results of hfg for bananas than other equations found in the literature.

关键词： Energy Agricultural Products Dried Banana Heat and mass Transfer

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Gut dysbiosis and systemic inflammation promote cardiomyocyte abnormalities in an experimental model of steatohepatitis

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World Journal of Hepatology 2021年第12期13卷 2052-2070页

作者： Larisse Longo Pabulo Henrique Rampelotto Eduardo Filippi-Chiela Valessa Emanoele Gabriel de Souza fernando Salvati Carlos Thadeu Cerski Themis Reverbel da Silveira Cláudia P Oliveira Carolina Uribe-Cruz mário ReisÁlvares-da-silva Experimental Laboratory of Hepatology and Gastroenterology Hospital de Clínicas de Porto AlegrePorto Alegre 90035-903Rio Grande do SulBrazil Graduate Program in Gastroenterology and Hepatology Universidade Federal do Rio Grande do SulPorto Alegre 90035-003Rio Grande do SulBrazil Graduate Program in Genetics and molecular Biology Universidade Federal do Rio Grande do SulPorto Alegre 90035-903Rio Grande do SulBrazil Center of Biotechnology Universidade Federal do Rio Grande do SulPorto Alegre 91501-970Rio Grande do SulBrazil Department of morphological Sciences Universidade Federal do Rio Grande do SulPorto Alegre 90050-170Rio Grande do SulBrazil Experimental Research Center Hospital de Clínicas de Porto AlegrePorto Alegre 90035-903Rio Grande do SulBrazil School of medicine Instituto Meridional de Educação-IMEDPasso Fundo 99070-220Rio Grande do SulBrazil Unit of Surgical Pathology Hospital de Clínicas de Porto AlegrePorto Alegre 90035-903Rio Grande do SulBrazil Department of Gastroenterology(LIm07) Faculdade de Medicina da Universidade de São PauloSão Paulo 01246903Brazil Division of Gastroenterology Hospital de Clínicas de Porto AlegrePorto Alegre 90035-903Rio Grande do SulBrazil

BACKGROUND Cardiovascular disease is the main cause of death in metabolic-associated fatty liver disease,and gut microbiota dysbiosis is associated with both of *** To assess the relationship between gut dysbiosis and cardiovascular risk(CVR)in an experimental model of *** Adult male Sprague-dawley rats were randomized to a control group(n=10)fed a standard diet and an intervention group(n=10)fed a high-fat choline-deficient diet for 16 ***,molecular,hepatic,and cardiac *** microbiota variables were *** The intervention group had a significantly higher atherogenic coefficient,Castelli’s risk index(CRI)-I and CRI-II,interleukin-1β,tissue inhibitor of metalloproteinase-1(all Pmonocyte chemoattractant protein-1(P=0.005),and plasminogen activator inhibitor-1(P=0.037)than the control *** expression of miR-33a increased(P=0.001)and miR-126(Pmputerized histological imaging analysis showed a significant decrease in the percentage of cardiomyocytes with a normal morphometric appearance(P=0.007),reduction in the mean area of cardiomyocytes(P=0.037),and an increase of atrophic cardiomyocytes(P=0.007).There were significant correlations between the cardiomyocyte morphometry markers and those of progression and severity of liver disease and *** intervention group had a lower Shannon diversity index and fewer changes in the structural pattern of gut microbiota(both Pmicrobial families that are involved in lipid metabolism were differentially abundant in intervention group and were significantly correlated with markers of liver injury and *** The study found a link between gut dysbiosis and significant cardiomyocyte abnormalities in animals with steatohepatitis.

关键词： Animal model Cardiovascular diseases Gut microbiota metabolic-associated fatty liver disease Predicted lipid metabolism Risk cardiovascular Steatohepatitis

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