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TOPLESS promotes plant immunity by repressing auxin signaling and is targeted by the fungal effector Naked1

Plant Communications 2022年第2期3卷 92-111页

作者： fernando navarrete Michelle Gallei Aleksandra EKornienko Indira Saado Mamoona Khan Khong-Sam Chia Martin A.Darino Janos Bindics Armin Djamei Gregor Mendel Institute(GMI) Austrian Academy of Sciences(OEAW)Vienna BioCenter(VBC)Dr.Bohr-Gasse 31030 ViennaAustria Leibniz Institute of Plant Genetics and Crop Plant Research(IPK) OT GaterslebenCorrensstraße 306466 Stadt SeelandGermany Present address:Institute of Science and Technology Austria Am Campus 13400 KlosterneuburgAustria Present address:Institute of Molecular Biotechnology Dr.Bohr-Gasse 31030 ViennaAustria Present address:Institute of Crop Science and Resource Conservation University of BonnBonnGermany

In plants,the antagonism between growth and defense is hardwired by hormonal *** perception of pathogen-associatedmolecularpatterns(PAMPs)frominvadingmicroorganismsinhibits auxin signalingand plant ***,pathogens manipulate auxin signaling to promote disease,but how this hormone inhibits immunity is not fully *** maydis is a maize pathogen that induces auxin signaling in its *** characterized a *** effector protein,Naked1(Nkd1),that is translocated into the host *** its native ethylene-responsive element binding factor-associated amphiphilic repression(EAR)motif,Nkd1 binds to the transcriptional co-repressors TOPLESS/TOPLESS-related(TPL/TPRs)and prevents the recruitment of a transcriptional repressor involved in hormonal signaling,leading to the derepression of auxin and jasmonate signaling and thereby promoting susceptibility to(hemi)biotrophic pathogens.A moderate upregulation of auxin signaling inhibits the PAMP-triggered reactive oxygen species(ROS)burst,an early defense ***,our findings establish a clear mechanism for auxin-induced pathogen *** Nkd1 variants with increased expression or increased EAR-mediated TPL/TPR binding trigger typical salicylic-acid-mediated defense reactions,leading to pathogen *** implies that moderate binding of Nkd1 to TPL is a result of a balancing evolutionary selection process to enable TPL manipulation while avoiding host recognition.

关键词： topless auxin pattern-triggered immunity PTI effector maize Ustilago maydis

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Prevalence and ecological factors affecting the distribution of plant-parasitic nematodes in Prunus groves in Spain

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Journal of Integrative Agriculture 2024年第2期23卷 566-589页

作者： Ilenia Clavero-Camacho Antonio Archidona-Yuste Carolina Cantalapiedra-navarrete Pablo Castillo Juan Emilio Palomares-Rius Institute for Sustainable Agriculture(IAS) Spanish National Research Council(CSIC)Córdoba 14004Spain Andalusian Institute of Agricultural and Fisheries Research and Training(IFAPA) Centro Alameda del ObispoCórdoba 14004Spain

A wide survey was conducted to study plant-parasitic nematodes(PPNs)associated with Prunus groves in *** research aimed to determine the prevalence and distribution of PPNs in Prunus groves,as well as the influence of explanatory variables describing soil,climate and agricultural management in structuring the variation of PPNs community composition.A total of 218 sampling sites were surveyed and 84 PPN species belonging to 32 genera were identified based of an integrative taxonomic *** species considered as potential limiting factors in Prunus production,such as Meloidogyne arenaria,***,***,Pratylenchus penetrans and ***,were identified in this *** soil physico-chemical(C,Mg,N,Na,OM,P,pH and clay,loamy sand and sandy loam texture classes),four climate(Bio04,Bio05,Bio13 and Bio14)and four agricultural management variables(grove-use history less than 10 years,irrigation,apricot seedling rootstock,and Montclar rootstock)were identified as the most influential variables driving spatial patterns of PPNs *** particular,younger plantations showed higher values for species richness and diversity indices than groves cultivated for more than 20 years with Prunus *** study increases the knowledge of the distribution and prevalence of PPNs associated with Prunus rhizosphere,as well as on the influence of explanatory variables driving the spatial structure PPNs communities,which has important implications for the successful design of sustainable management strategies in the future in this agricultural system.

关键词： nematodes Meloidogyne Pratylenchus Paratylenchus rootstock distribution soil grove-use almond peach

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Distribution and Relation of Primes with Tesla Numbers 3, 6, and 9

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Journal of Applied Mathematics and Physics 2024年第4期12卷 1021-1027页

作者： Javier Mendoza-navarrete Huetzin Perez Ismael Ojeda Victor Verdugo Gabriel Luna Sandoval Ricardo Jimenez Garcia Jaime Alvarez Departametno de Matemá ticas CBTis 256 DGETI Cabo San Lucas M&#233xico DCI Departamento de Fisica Mé dica Universidad de Guanajuato Leon M&#233xico Divisió n de Ingenieria en Sistemas Computacionales Tecnol&#243gico Nacional de M&#233xico ITES Los Cabos Cabo San Lucas M&#233xico Departamento de Ingenieria Industrial en Manufactura Universidad Estatal de Sonora Navojoa M&#233xico Departamento de Elé ctrica-Electr&#243nica Tecnol&#243gico Nacional de M&#233xico Campus Mexicali Mexicali M&#233xico Guanajuato M&#233xico

This work presents a different approach to twin primes, an approach from the perspective of the Tesla numbers and gives a refresh and new observation of twin primes that could lead us to an answer to the Twin Prime Conjecture problem. We expose a peculiar relation between twin primes and the generation of prime numbers with Tesla numbers. Tesla numbers seem to be present in so many domains like time, vibration and frequency [1], and the space between twin primes is not the exception. Let us say that twin primes are more than just prime numbers plus 2 or minus 2, and Tesla numbers are more involved with twin primes than we think, and hopefully, this approach give us a better understanding of the distribution of the twin pairs.

关键词： Tesla Numbers Prime Number Twin Prime Numbers Twin Pair

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The Nikola Tesla Constant and Its Relation to the Circumference Inscribed in a Square

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Journal of Applied Mathematics and Physics 2024年第1期12卷 277-283页

作者： Javier Mendoza navarrete José Ismael Ojeda Campaña Huetzin Perez Gabriel Luna-Sandoval Victor Verdugo Ricardo Jiménez Garcia Departamento de Matemáticas CBTis 256 DGETI Cabo San Lucas México División de Ingeniería en Sistemas Computacionales Tecnológico Nacional de México/ITES de Los Cabos Cabo San Lucas México DCI Departamento de Física Médica Universidad de Guanajuato León México Departamento de Ingeniería Industrial en Manufactura Universidad Estatal de Sonora Navojoa México Departamento de Eléctrica-Electrónica Tecnológico Nacional de México Campus Mexicali Mexicali México

This research work relates the surface of a square and the area circumscribed by a circle, resulting in a value called Nikola Tesla constant. This constant starts with the calculation of the areas of the square and the inscribed circle, giving a ratio of 9/40 and from which a residual area of the area proportions of the geometric figures described is obtained. Plotting smooth curves, particularly those in round shapes, can be represented efficiently with the use of Nikola Tesla constant, reducing complex mathematical calculus.

关键词： Tesla Numbers Prime Number Circle Binary Area

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Luminal oxidants selectively modulate electrogenic ion transport in rat colon

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World Journal of Gastroenterology 2006年第34期12卷 5523-5527页

作者： Julio M Mayol Yolanda Adame-navarrete Pilar Alarma-Estrany Elena Molina-Roldan fernando Huete-Toral Jesus A Fernandez-Represa Servicio De Cirugia I Hospital Clinico San Carlos Universidad Complutense De Madrid Madrid 28040 Spain

AIM： To investigate the effects of luminal exposure to H2O2 and two related thiol oxidizing agents on basal and stimulated chloride secretion in native colon using electrophysiological and pharmacological approaches. METHODS： Unstripped rat distal colon segments were mounted in Ussing chambers. Potential difference, calculated resistance and short-circuit current across unstripped colon segments were monitored with a dual voltage/current clamp. Paracellular permeability was assessed by measuring the mucosa-to-serosa flux of a fluorescent probe （FITC）. RESULTS： Luminal exposure to hydrogen peroxide transitorily stimulated chloride secretion without altering barrier function. This stimulatory effect could be blocked by basolateral atropine but not indomethacin. The cysteine and methionine oxidizing compounds, phenylarsine oxide and chloramine T respectively, mimicked the effect of H2O2, except for a drop in transcolonic resistance after 30 min. In contrast to the observed stimulatory effect on basal secretion, cAMP-stimulated electrogenic ion transport was blunted by luminal H2O2. However, the Ca^2＋- activated response remained unchanged. CONCLUSION： H2O2 may be an important selective modulator of intestinal ion and water secretion in certain pathologic conditions such as inflammation or ischemiareperfusion by multiple mechanisms.

关键词： Chloride secretion Ion transport Rat distal colon Hydrogen peroxide Acetylcholine Atropine

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Epidemiologic characteristics of death by burn injury from 2000 to 2009 in Colombia, South America:a population-based study

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Burns & Trauma 2016年第1期4卷 60-67页

作者： Norberto navarrete Nelcy Rodriguez Burn Intensive Care Unit Simón Bolívar HospitalBogotáColombia. ClinicalEpidemiology and Biostatistics Department Javeriana UniversityBogotáColombia. Department of Statistics National University of ColombiaBogotáColombia.

Background:Burns are one of the most severe traumas that an individual can *** World Health Organization(WHO)affirms that injuries related to burns are a global public health problem mainly in low-and middle-income *** first step towards reducing any preventable injury is based on accurate *** Colombia,the basic epidemiological characteristics of burn injuries are *** objectives were establishing the causes,high-risk populations,mortality rate,and tendencies of burn ***:Observational,analytical,population-based study based on official death certificate occurred between 2000 and *** codes of the International Classification of Diseases-10th Revision(ICD-10)related to burns were *** mortality rates were standardized using the WHO world average age weights 2000–*** determine the tendency,an average annual percent change(AACP)was ***:A total of 5448 deaths due to burns were identified;78.4%were *** crude and adjusted burn mortality rate was 1.270 and 1.302 per 100,000,*** AACP was?5.25%.Electrical injury caused the greatest number of deaths(49.5%),followed by fire and lightning injuries.A total of 1197(22.1%)children were under 15 years *** causes of deaths were different among age groups.59.4%deaths occurred outside health ***:This study is a first step in identifying the main causes of death and groups with higher mortality *** is the main cause of deaths due to burn *** research is required in order to generate awareness among government and community for reducing the number of injuries and burn deaths in our country.

关键词： injuries injury characteristics

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Current applications of artificial intelligence for intraoperative decision support in surgery Allison

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Frontiers of Medicine 2020年第4期14卷 369-381页

作者： J.navarrete-Welton Daniel A.Hashimoto Surgical Artificial Intelligence and Innovation Laboratory Massachusetts General HospitalBostonMA 02114USA Harvard Medical School BostonMA 02114USA

Research into medical artificial intelligence(AI)has made significant advances in recent years,including surgical *** scoping review investigated AI-based decision support systems targeted at the intraoperative phase of surgery and found a wide range of technological approaches applied across several surgical *** the twenty-one(n=21)included papers,three main categories of motivations were identified for developing such technologies:(1)augmenting the information available to surgeons,(2)accelerating intraoperative pathology,and(3)recommending surgical *** many of the proposals hold promise for improving patient outcomes,important methodological shortcomings were observed in most of the reviewed papers that made it difficult to assess the clinical significance of the reported performance *** limitations,the current state of this field suggests that a number of opportunities exist for future researchers and clinicians to work on AI for surgical decision support with exciting implications for improving surgical care.

关键词： artificial intelligence decision support clinical decision support systems intraoperative deep learning computer vision machine learning surgery

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Application of wave equation theory to improve dynamic cone penetration test for shallow soil characterisation

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Journal of Rock Mechanics and Geotechnical Engineering 2022年第1期14卷 289-302页

作者： Miguel Angel Benz navarrete Pierre Breul Roland Gourvès Sol Solution Géotechnique Réseaux ZA des Portes de Riom Nord23 Avenue Georges GershwinRiom Cedex63204France UniversitéClermont Auvergne Institut PascalUMR CNRS 6602Clermont-Ferrand63000France Polytech Clermont-Ferrand Campus des Cézeaux 2 Avenue Blaise PascalAubière Cedex63178France

Among the geotechnical in situ tests,the dynamic penetration test(DPT)is commonly used around the ***,DPT remains a rough technique and provides only one failure parameter:blow count or cone *** paper presents an improvement of the dynamic cone penetration test(DCPT)for soil characterisation based on the wave equation *** on an instrumented lightweight dynamic penetrometer driving with variable energy,the main process of the test involves the separation and reconstruction of the waves propagating in the rods after each blow and provides a dynamic cone load-penetration(DCLT)*** analytical methodology is used to analyse this curve and to estimate additional strength and deformation parameters of the soil:dynamic and pseudo-static cone resistances,deformation modulus and wave *** carried out in the laboratory on different specimens(wood,concrete,sand and clay)in an experimental sand pit and in the field demonstrated that the resulting DCLT curve is reproducible,sensitive and reliable to the test conditions(rod length,driving energy,etc.)as well as to the soil properties(nature,density,etc.).Obtained results also showed that the method based on shock polar analysis makes it possible to evaluate mechanical impedance and wave velocity of soils,as demonstrated by the comparisons with cone penetration test(CPT)and shear wave velocity measurements made in the *** technique improves the method and interpretation of DPT and provides reliable data for shallow foundation design.

关键词： In situ test Dynamic cone penetrometer P.A.N.D.A. Wave equation Wave decoupling Dynamic cone load-penetration(DCLT)curve

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Total Petroleum Hydrocarbon Degradation by Endophytic Fungi from the Ecuadorian Amazon

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Advances in Microbiology 2018年第12期8卷 1029-1053页

作者： fernando Marín Hugo navarrete Alexandra Narvaez-Trujillo Center for Research on Health in Latin America (CISeAL) College of Exact and Natural Sciences PUCE Quito Ecuador School of Biological Sciences PUCE Quito Ecuador

The capacity of 133 fungal endophyte isolates for degrading petroleum hydrocarbons was evaluated. The endophytes were isolated from leaf and stem tissues from 23 plants collected in a natural habitat contaminated with crude oil in southwestern Ecuador. Their capacity for hydrocarbon biodegradation was tested by an in vitro colorimetric qualitative test during 10 days, using the Minimal Salt Medium and crude oil as the carbon source. Taxonomic identification of the endophytic fungi that showed bioactivity in the qualitative test was carried out by analysis of the ITS gene of the region 18S of the rDNA. Endophytes showed the best results in the previous qualitative test where selected for a quantitative in vitro test for 30 days. Residual hydrocarbons were tracked by infrared spectroscopy (IR) and gas chromatography (GC) with a flame ionization detector. The maximum removal rates of total petroleum hydrocarbons were 99.6% (IR) and 99.8% (GC), corresponding to fungi of the genus Verticillium sp. and Xylaria sp. 1 respectively. This is the first report of biodegradation of crude oil hydrocarbons by endophytic fungi in a tropical ecosystem. The results suggest these fungal isolates are potential hydrocarbon biodegraders that could be used in bioremediation processes.

关键词： Fungal Endophyte Biodegradation Bioremediation Hydrocarbon Infrared Spectroscopy Gas Chromatography

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The improvement of an object-oriented classification using multi-temporal MODIS EVI satellite data

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International Journal of Digital Earth 2009年第3期2卷 219-236页

作者： Y.Gao J.-F.Mas A.navarrete Centro de Investigaciones en Ecosistemas-Universidad Nacional Autonoma de Mexico(UNAM) Antigua Carretera a Pa tzcuaro No.8701Col.Ex Hacienda de San Jose de la Huerta C.P.58190 MoreliaMichoacanMexico Centro de Investigaciones en Geografıa Ambiental-Universidad Nacional Autonoma de Mexico(UNAM) Antigua Carretera a Pa tzcuaro No.8701Col.Ex Hacienda de San Jose de la Huerta C.P.58190 MoreliaMichoacanMexico

This paper investigates the contribution of multi-temporal enhanced vegetation index(EVI)data to the improvement of object-based classification accuracy using multi-spectral moderate resolution imaging spectral-radiometer(MODIS)*** object-oriented classification,similar pixels are firstly grouped together and then classified;the produced result does not suffer the speckled appearance and closer to human *** data are from the MODIS sensor aboard Terra spacecraft.69 EVI data(scenes)were collected during the period of three years(20012003)in a mountainous vegetated *** data sets were used to study the phenology of the land cover *** land cover types show distinct fluctuations over time in EVI values and this information might be used to improve object-oriented land cover *** experiments were carried out:one was only with single date MODIS multispectral data,and the other one including also the 69 EVI *** classes were distinguished:temperate forest,tropical dry forest,grassland,irrigated agriculture,rain-fed agriculture,orchards,lava flows and human *** two classifications were evaluated with independent verification data,and the results showed that with multitemporal EVI data,the classification accuracy was improved 5.2%.Evaluated by McNemar’s test,this improved was significant,with significance level p=0.01.

关键词： enhanced vegetation index(EVI) moderate resolution imaging spectral-radiometer(MODIS) phenology object-based image classification

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