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BIOCELL 2020年第3期44卷 293-299页

作者： MARÍA ELISA MILLÁN MARÍA fernanda MARRA LEONARDO ANDRÉS SALVARREDI EMILIO FERNANDO LIZARRAGA LUIS ALBERTO LOPEZ Universidad Nacional de Cuyo MendozaArgentina Comisión Nacional de Energía Atómica Buenos AiresArgentina Fundación Escuela de Medicina Nuclear MendozaArgentina Fundación Miguel Lillo y Universidad Nacional de Tucumán San Miguel de TucumánArgentina

The ajenjo,Artemisia mendozana ***(Asteraceae),grows in the Andean foothills of Mendoza and San Juan,Argentina,and is used as a medicinal plant for its antispasmodic and antifungal *** aim of this work was to obtain fractions of a chloroform extract of ajenjo leaves and to evaluate the in vitro effects on proliferation,viability and clonogenicity of B16-F0 melanoma *** a silica gel chromatography column,120 fractions were collected and grouped according to the chromatographic profile in 9 main fractions(F1–F9).Their major compounds identified were:terpenes(F1),terpenes and sesquiterpene lactones(F2–F3),sesquiterpenes(F4–F6)and phenols and sesquiterpenes(F7-9).B16-F0 cells were incubated for 72 h with DMSO(vehicle)or 0.1 mg/ml F1–*** 72 h of culture,F1 decreased both the growing index(GI)and cell viability.F2 and F3 both decreased GI and only F3 decreased clonogenic activity.F4 and F5 both decreased *** F5 decreased cell viability and F4 decreased ***,fractions F6–F8 did not affect any of the cell parameters assayed,while F9 decreased cell viability and inhibited clonogenicity.

关键词： Terpenes Sesquiterpene lactones Sesquiterpenes Phenols

Nursing Diagnosis in Patients with Liver Cirrhosis in Use of Feeding Tube

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Open Journal of Nursing 2016年第7期6卷 505-514页

作者： fernanda Raphael Escobar Gimenes Patrícia Costa dos Santos da Silva Andréia Regina Lopes Renata Karina Reis Rebecca Shasanmi Emília campos de Carvalho Department of General and Specialized Nursing University of S&atildeo Paulo at Ribeir&atildeo Preto College of Nursing Ribeir&atildeo Preto Brazil Federal University of Uberlandia Uberlandia Brazil Academy of the Brazilian Air Force Pirassununga Brazil Nursing and Public Health Research Philadelphia PA USA

The objective was to identify the most frequent nursing diagnoses labels in patients with liver cirrhosis in use of feeding tube. A descriptive research was carried out in a Brazilian Hospital with 20 adult patients. Systematic data collection utilized the Conceptual Model of Wanda Horta, the first nurse to introduce the concept of Nursing Process in Brazil. The six phases of the nursing diagnostic reasoning proposed by Risner were used;nursing diagnoses were described according to NANDA-I taxonomy II. Patients were mainly male;half of them were middle age adults;they had an average of 12.8 nursing diagnoses labels;and the most frequent were: risk for aspiration and risk for infection. Nurses needed to develop effective skills to properly diagnose in order to provide safe care and improve patient outcomes.

关键词： Nursing Diagnosis Enteral Nutrition Liver Cirrhosis

Activation of stress-response genes by retrograde signaling-mediated destabilization of nuclear importin IMPα-9 and its interactor TPR2

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Molecular Plant 2024年第6期17卷 884-899页

作者： Liping Zeng Maria fernanda Gomez Mendez Jingzhe Guo Jishan Jiang Bailong Zhang Hao Chen Brandon Le Haiyan Ke Katayoon Dehesh Department of Botany and Plant Sciences Institute of Integrative Genome BiologyUniversity of CaliforniaRiversideRiversideCA 92521USA School of Medicine University of CaliforniaRiversideRiversideCA 92521USA

Stress-induced retrograde signal transmission from the plastids to the nucleus has long puzzled plant *** address this,we performed a suppressor screen of the ceh1 mutant,which contains elevated 2-C-methyl-d-erythritol-2,4-cyclopyrophosphate(MEcPP)levels,and identified the gain-of-function mutant impα-9,which shows reversed dwarfism and suppressed expression of stress-response genes in the ceh1 background despite heightened *** genetic and biochemical analyses established that the accumulation of MEcPP initiates an upsurge in Arabidopsis SKP1-like 1(ASK1)abundance,a pivotal component in the proteasome degradation *** increase in ASK1 prompts the degradation of IMPα-***,we uncovered a protein-protein interaction between IMPα-9 and TPR2,a transcriptional co-suppressor and found that a reduction in IMPα-9 levels coincides with a decrease in TPR2 ***,the interaction between IMPα-9 and TPR2 was disrupted in impα-9 mutants,highlighting the critical role of a single amino acid alteration in maintaining their *** of their interaction results in the reversal of MEcPP-associated *** immunoprecipitation coupled with sequencing analyses revealed that TPR2 binds globally to stress-response genes and suggested that IMPα-9 associates with the *** function together to suppress the expression of stress-response genes under normal conditions,but this suppression is alleviated in response to stress through the degradation of the suppressing *** biological relevance of our discoveries was validated under high light stress,marked by MEcPP accumulation,elevated ASK1 levels,IMPα-9 degredation,reduced TPR2 abundance,and subsequent activation of a network of stress-response *** summary,our study collectively unveils fresh insights into plant adaptive mechanisms,highlighting intricate interactions among retrograde signaling,the proteasome,and nuclear transport machinery.

关键词： plastidial retrograde signal MEcPP ASK1 impα-9 TPR2

Intravenous vs intraperitoneal mesenchymal stem cells administration:What is the best route for treating experimental colitis?

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World Journal of Gastroenterology 2014年第48期20卷 18228-18239页

作者： Fabiany da Costa Goncalves Natália Schneider fernanda Otesbelgue Pinto Fabíola Schons Meyer fernanda Visioli Bianca Pfaffenseller Patrícia Luciana da Costa Lopez Eduardo Pandolfi Passos Elizabeth Obino Cirne-Lima Luíse Meurer Ana Helena Paz Centro de Pesquisa Experimental do Hospital de Clínicas de Porto Alegre Porto Alegre 90035-903Rio Grande do SulBrazil Programa de Pós-Gradua??o:Ciências em Gastroenterologia e Hepatologia Universidade Federal do Rio Grande do SulPorto Alegre 90035-903Rio Grande do SulBrazil Faculdade de Odontologia Universidade Federado Rio Grande do SulPorto Alegre 90035-003Rio Grande doSulBrazil

AIM:To investigate the therapeutic effects of mesenchymal stem cells(MSCs)transplanted intraperitoneally andintravenously in a murine model of ***:MSCs were isolated from C57BL/6 mouse adipose *** cultures were analyzed according to morphology,cellular differentiation potential,and surface molecular *** acute colitis was induced in C57BL/6 mice by oral administration of2%dextran sulfate sodium(DSS)in drinking water ad libitum from days 0 to *** mice were treated with1×106 MSCs via intraperitoneal or intravenous injection on days 2 and *** disease activity index was determined daily based on the following parameters:weight loss,stool consistency and presence of blood in the feces and *** compare morphological and functional differences in tissue regeneration between different MSC injection modalities,mice were euthanized on day 8,and their colons were examined for length,weight,and histopathological *** responses were determined by measuring the levels of different serum cytokines using a CBA Th1/Th2/Th17 *** rates were evaluated by terminal deoxynucleotidyl transferase-mediated d UDPbiotin nick end labeling ***:Intravenous infusion of MSCs was more effective than intraperitoneal treatment(P<0.001)in reducing the clinical and histopathologic severity of colitis,which includes weight loss,diarrhea and *** histological evaluation demonstrated decreased colonic inflammation based on reduced crypt loss and reduced infiltration of inflammatory *** therapeutic effect was most likely mediated by the down-regulation of pro-inflammatory cytokines[interleukin(IL)-6 and tumor necrosis factor(TNF)];and by the up-regulation of anti-inflammatory cytokines(IL-10 and IL-4).Intravenous transplantation alsoinduced high levels of IFN that lead to activation of the immunosuppressive activity of the MSCs,which did not occur with intraperitoneal transplantation(P=0.006).An increase in a

关键词： Ulcerative colitis Dextran sulfate sodium Inflammatory bowel disease Mesenchymal stem cell Cell transplantation Intravenous injection Immunomodulation

Sirtuins in B lymphocytes metabolism and function

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World Journal of Experimental Medicine 2019年第1期9卷 1-13页

作者： Bruno Ghirotto fernanda Fernandes Terra Niels Olsen Saraiva Camara Paulo José Basso Department of Immunology Institute of Biomedical Sciences University of Sao Paulo Division of Nephrology School of Medicine Federal University of Sao Paulo Laboratory of Renal Physiology (LIM 16) School of MedicineUniversity of S?o Paulo

Sirtuins(SIRTs) are NAD+-dependent histone deacetylases and play a role in virtually all cell biological processes. As SIRTs functions vary according to their subtypes, they can either activate or inhibit signaling pathways upon different conditions or tissues. Recent studies have focused on metabolic effects performed by SIRTs in several cell types since specific metabolic pathways(e.g., aerobic glycolysis, oxidative phosphorylation, β-oxidation, glutaminolysis) are used to determine the cell fate. However, few efforts have been made to understand the role of SIRTs on B lymphocytes metabolism and function. These cells are associated with humoral immune responses by secreting larger amounts of antibodies after differentiating into antibody-secreting cells. Besides, both the SIRTs and B lymphocytes are potential targets to treat several immune-mediated disorders, including cancer. Here, we provide an outlook of recent studies regarding the role of SIRTs in general cellular metabolism and B lymphocytes functions, pointing out the future perspectives of this field.

关键词： B cells Metabolic sensors Histone deacetylases Cancer

An eighteen-month follow-up study on the effects of Intravitreal Dexamethasone Implant in diabetic macular edema refractory to anti-VEGF therapy

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International Journal of Ophthalmology(English edition) 2016年第10期9卷 1427-1432页

作者： fernanda Pacella Maria Rosaria Romano Paolo Turchetti Giovanna Tarquini Anna Carnovale Antonella Mollicone Alessandra Mastromatteo Elena Pacella Department of Sense Organs Faculty of Medicine and DentistrySapienza University of Rome IRCCS NeuromedPozzilli National Institute for Health MigrationPoverty(INMP/NIHMP) Department of Internal Medicine and Clinical Specialities Sapienza University of Rome

AIM： To evaluate the long-term efficacy and safety of dexamethasone implants in subjects affected by diabetic macular edema（DME） resistant to anti-vascular endothelial growth factor（VEGF） ***： Thirty-two DME patients were enrolled.A700 microgram slow release Intravitreal Dexamethasone Implant（Ozurdex~） was placed in the vitreous *** patients were followed for 18 ***-corrected visual acuity（BCVA） measured with Early Treatment Diabetic Retinopathy Study（ETDRS） and central macular thickness（CMT） exams were carried out at baseline（T0）and after 1（T1）,3（T3）,4（T4）,6（T6）,9（T9）,12（T12）,15（T15）,and 18mo（T18） post injection. RESULTS： Repeated measures ANOVA showed an effect of treatment on ETDRS（P〈0.0001）.Post hoc analyses revealed that ETDRS values were significantly increased at T1,T3,T4,T9,and T15（P 〈0.001） as compared to baseline value（T0）.At T6,T12,and T18,ETDRS values were still statistically higher than baseline（P〈0.001 vs T0）.However,at these time points,we observed a trend to return to baseline *** also showed an effect of treatment（P 〈0.0001）.CMT decreased significantly at T1,T3,T4,T9,and T15（P〈0.001）.At T6（P〈0.01）,T12 and T18（P〈0.001） CMT was also significantly lower than T0 although a trend to return to the baseline conditions was also ***： Our findings demonstrate that Intravitreal Dexamethasone Implant is a good option to improveBCVA and CMT in DME patients resistant to anti-VEGF *** data also show that the use of drugs administered directly into the vitreous allows achieving appropriate and long-lasting concentration at the site of disease without systemic side effects.

关键词： diabetic macular edema Intravitreal Dexamethasone Implant anti-vascular endothelial growth factor therapy

Methods and parameters of melting curve analysis for identification of Leishmania species:A scoping review

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Asian Pacific Journal of Tropical Medicine 2021年第12期14卷 528-542页

作者： Juliana J G Ferreira fernanda S Nascimento Glaucia E B Marcon Eros A de Almeida Sandra C B Costa Laboratório de Diagnóstico de Doenças Infecciosas por Técnicas de Biologia Molecular Departamento de Clínica MédicaFaculdade de Ciências MédicasUniversidade Estadual de Campinas(UNICAMP)Campinas13083-887SPBrazil Fundação Oswaldo Cruz Mato Grosso do Sul(FIOCRUZ MS) Rua Gabriel Abrão92Jardim das NaçõesCampo Grande79081-746 MSBrazil Grupo de Estudos em Doença de Chagas Departamento de Clínica MédicaFaculdade de Ciências MédicasUniversidade Estadual de Campinas(UNICAMP)Campinas13083-887SPBrazil

Leishmaniasis is a set of diseases with a worldwide distribution that affects mainly economically underprivileged populations in developing countries. It has a major impact on public health, with a global cost of billions of dollars per year. The treatment and control of leishmaniasis vary according to the Leishmania species involved, which require reliable methods for species identification. Since most of the currently used methods have limitations, there is a need for assays that allow rapid, precise identification of the offending species. Real-time polymerase chain reactions in conjunction with dissociation curve analysis have been used to detect differences in the DNA composition of selected genes of Leishmania spp. Kinetoplast DNA is the main molecular target used because of its high copy number per parasite, but other targets have also been studied. As part of an effort to establish melting temperature standards for each target gene, we have reviewed the pertinent literature available in public databases, including Pub Med, Web of Science, Sci ELO and LILACS, using the keywords "Leishmania", "leishmaniasis", "realtime PCR", "melting temperature", and "melting curve", alone or in combination. After applying eligibility criteria, 27 articles were selected for analysis. A considerable variation in the methodologies analyzed was found regarding molecular targets, standardization of the methods, reproducibility and specificity. Because of this, statistical analysis was not performed. In most cases, the methods were able to differentiate the parasite at the subgenus level or few species regardless of the target chosen.

关键词： Leishmaniasis Melting temperature Real-time PCR

Association of swine influenza H1N1 pandemic virus(SIV-H1N1p) with porcine respiratory disease complex in sows from commercial pig farms in Colombia

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Virologica Sinica 2014年第4期29卷 242-249页

作者： Luisa fernanda Mancipe Jiménez Gloria Ramírez Nieto Victor Vera Alfonso Jairo Jaime Correa Laboratory of Animal Virology Faculty of Veterinary Medicine and Animal Science National University of Colombia

Porcine respiratory disease complex （PRDC） is a serious health problem that mainly affects growing and finishing pigs. PRDC is caused by a combination of viral and bacterial agents, such as porcine reproductive and respiratory syndrome virus （PRRSV）, swine influenza virus （SIV）, Mycoplasma hyopneumoniae （Myh）, Actinobacillus pleuropneumoniae （APP）, Pasteurella multocida and Porcine circovirus 2 （PCV2）. To characterize the specific role of swine influenza virus in PRDC presentation in Colombia, 11 farms from three major production regions in Colombia were examined in this study. Nasal swabs, bronchial lavage and lung tissue samples were obtained from animals displaying symptoms compatible with SIV. Isolation of SIV was performed in 9-day embryonated chicken eggs or Madin-Darby Canine Kidney （MDCK） cells. Positive isolates, identified via the hemagglutination inhibition test, were further analyzed using PCR. Overall, 7 of the 11 farms were positive for SIV. Notably, sequencing of the gene encoding the hemagglutinin （HA） protein led to grouping of strains into circulating viruses identified during the human outbreak of 2009, classified as pandemic H1N1-2009. Serum samples from 198 gilts and multiparous sows between 2008 and 2009 were obtained to determine antibody presence of APP, Myh, PCV2 and PRRSV in both SIV-H1Nlp-negative and -positive farms, but higher levels were recorded for SIV- HI Nlp-positive farms. Odds ratio （OR） and P values revealed statistically significant differences （p〈0.05） in PRDC presentation in gilts and multiparous sows of farms positive for SIV-HINlp. Our findings indicate that positive farms have increased risk of PRDC presentation, in particular, PCV2, APP and Myh.

关键词： SIV-HIN1 swine influenza pandemic porcine respiratory disease complex Colombia

Mg-based materials diminish tumor spreading and cancer metastases

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Bioactive Materials 2023年第1期19卷 594-610页

作者： Philipp Globig Roshani Madurawala Regine Willumeit-Römer fernanda Martini Elisa Mazzoni Bérengère J.C.Luthringer-Feyerabend Institute of Metallic Biomaterials Helmholtz-Zentrum Hereon GmbH21502GeesthachtGermany Department of Medical Sciences University of Ferrara44121FerraraItaly

Cancer metastases are the most common causes of cancer-related *** formation of secondary tumors at different sites in the human body can impair multiple organ function and dramatically decrease the survival of the *** this stage,it is difficulty to treat tumor growth and spreading due to arising therapy ***,it is important to prevent cancer metastases and to increase subsequent cancer therapy *** metastases are conventionally treated with radiation or ***,these treatments elicit lots of side effects,wherefore novel local treatment approaches are currently *** studies already showed anticancer activity of specially designed degradable magnesium(Mg)alloys by reducing the cancer cell *** this work,we investigated the impact of these Mg-based materials on different steps of the metastatic cascade including cancer cell migration,invasion,and cancer-induced ***,Mg and Mg-6Ag reduced cell migration and invasion of osteosarcoma cells in coculture with ***,the Mg-based materials used in this study diminished the cancer-induced *** cells incubated with conditioned media obtained from these Mg and Mg-6Ag showed a reduced cell layer permeability,a reduced proliferation and inhibited cell *** tube formation as a last step of angiogenesis was stimulated with the presence of Mg under normoxia and diminished under hypoxia.

关键词： Cancer Osteosarcoma Magnesium degradation Cell migration Cell invasion Angiogenesis

How to Ensure Food Supply to the Population of the Medieval Village of Loule (1384-1488)

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Journal of Food Science and Engineering 2019年第7期9卷 255-265页

作者： Luisa fernanda Guerreiro Martins Interdisciplinary History Cultures and Societies Centre of the University of Evora(UID/HIS/00057/2013POCI-01-0145-FEDER-007702)Largo do Marques de Marialvan. 8Apartado 94Evora 7000-809Portugal

Located in the centre of the Algarve,in the South of Portugal,the ancient village of Louléhas its origins in a Muslim medieval medina(8th Century).After the Christian Conquest of the South and namely of the Louléterritory in 1249,the Christian Administration,based on the city councils meetings,composed of good men,will have to reconcile the social and religious dynamics of the populations who stayed in the territory,formed by Jews,Muslims and *** this social,cultural and religious scenario,local administration will also assure the management of production,distribution and sale of food needed to ensure the survival and the settlement of the populations in the *** of that distribution included cereals,olive oil,wine,meat and fish,all of them representing the Mediterranean food basis of the populations from the South of the Iberian *** is about this daily concern with food distribution and consumption,in a religious and culturally tripartite society that we intend to present this article,focusing the analysis on three types of food:cereals(“bread”),meat and *** intend to demonstrate how the medieval council of Loulémanaged the production of the food and how the population was fed,using the Councillor Minutes of the LouléCity Council in the medieval Christian *** the documentation allows us to propose an interpretation for the consecrated trilogy of bread,olive oil and wine,we choose to underline three fundamental areas of the economy of the Algarve region:cattle raising and the supply of meat to the populations,the shortage of cereals with which the“bread”supply was ensured and the commercialization of *** provision of meat,cereals and fish ensured that the population was well fed,but the most interesting is that,the way the food distribution was made,allows us to understand the capacity to accept a tripartite society between“Christians”,“Moors”and“Jews”.

关键词： Mediterranean food medieval city religious scenario food production distribution.