检索结果-南通市图书馆

不同水平蔗糖对泌乳奶牛体外产气及泌乳的影响

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

中国乳业 2024年第5期 57-61页

作者：刘凤泉赤峰市巴林左旗农牧技术推广中心内蒙古赤峰025450

[目的]研究不同蔗糖添加水平对泌乳奶牛体外产气、泌乳量、乳汁成分的影响。[方法]于内蒙古自治区赤峰市巴林左旗某规模化奶牛场随机选择90头(2~3)胎次、体重和年龄相近泌乳奶牛为研究对象,随机分为3组,分别为对照组、试验I组和试验II组... 详细信息

[目的]研究不同蔗糖添加水平对泌乳奶牛体外产气、泌乳量、乳汁成分的影响。[方法]于内蒙古自治区赤峰市巴林左旗某规模化奶牛场随机选择90头(2~3)胎次、体重和年龄相近泌乳奶牛为研究对象,随机分为3组,分别为对照组、试验I组和试验II组,每组30头。对照组饲喂常规全混合日粮,试验I组和试验II组在饲喂常规全混合日粮基础上,添加2%、4%水平蔗糖,试验周期65 d。比较试验结束后奶牛体外产气量、泌乳量、乳汁成分。[结果]试验II组发酵48 h产气量、理论最大产气量、达到最大产气量1/2时的产气速率,高于试验I组和对照组(P0.05)。对照组、试验I组和试验II组脂蛋比、乳蛋白率、乳脂率、尿素氮、体细胞数等乳汁成分含量比较无显著差异(P>0.05)。[结论]泌乳奶牛常规全混合日粮中添加蔗糖,可在不影响瘤胃发酵模式基础上,加快体外产气速率,不影响产奶量、乳汁成分。4%蔗糖添加量效果最佳。

关键词：蔗糖泌乳奶牛体外产气产奶量乳汁成分

维普期刊数据库博看期刊

植物病原细菌基因敲除技术在本科开放实验中的设计与实践

在线全文

学校读者我要写书评

暂无评论

科教导刊 2024年第1期 67-69页

作者：李开怀刘凤权贵州大学农学院植物病理学教研室贵州贵阳550025

基因敲除技术在植物病原细菌和寄主互作研究中发挥着重要作用。近年来,该技术在植物病理学研究领域得到了广泛应用。文章以植物病原细菌基因敲除技术为教学内容,以构建野油菜黄单胞菌(Xanthomonas campestris ***)的RNA聚合酶σ54因子... 详细信息

基因敲除技术在植物病原细菌和寄主互作研究中发挥着重要作用。近年来,该技术在植物病理学研究领域得到了广泛应用。文章以植物病原细菌基因敲除技术为教学内容,以构建野油菜黄单胞菌(Xanthomonas campestris ***)的RNA聚合酶σ54因子编码基因rpoN2的突变体为例,探讨了基因敲除技术在本科开放实验教学中的设计与实践。通过rpoN2基因敲除载体构建、野油菜黄单胞菌的遗传转化以及菌落PCR筛选重组子等多方面的教学内容的设计与实践,提高了学生对植物病原细菌基因敲除原理的理解和掌握,培养了学生相关实验操作技能、数据分析和解读能力,以及团队合作精神和科学探究意识。

关键词：植物病原细菌基因敲除技术本科教育实验课程

维普期刊数据库博看期刊

在线全文

学校读者我要写书评

暂无评论

柞蚕精子质量与各虫态生命力关系研究

蚕业科学 2024年第1期50卷 91-96页

作者：陈欣陈俊山徐亮刘佩锋刘书禹刘凤全谌苗苗辽宁省蚕业科学研究所辽宁凤城118100

为探索柞蚕精子质量对虫体生命力的影响,以5个二化性品种柞蚕为试材,测定交配期精子质量,并在卵期、幼虫期、成虫期3个变态阶段,对柞蚕进行抗性鉴定。结果表明:柞蚕有核精子受精能力和无核精子活动能力在品种之间存在显著差异,有核精子... 详细信息

为探索柞蚕精子质量对虫体生命力的影响,以5个二化性品种柞蚕为试材,测定交配期精子质量,并在卵期、幼虫期、成虫期3个变态阶段,对柞蚕进行抗性鉴定。结果表明:柞蚕有核精子受精能力和无核精子活动能力在品种之间存在显著差异,有核精子受精能力与卵期耐高温胁迫能力呈显著正相关,与低龄幼虫对ApNPV的抗性呈显著正相关;无核精子活动能力与成虫耐高温胁迫能力呈显著正相关。说明柞蚕精子质量在一定程度上决定了卵期和幼虫期生命力,且成虫期生命力强的雄蛾产生的精子质量也相对较高。研究结果可为选育强健型柞蚕品种提供参考。

关键词：柞蚕精子质量卵期生命力幼虫期生命力成虫期生命力

同方期刊数据库

A war on the cell wall

在线全文

学校读者我要写书评

暂无评论

Molecular Plant 2022年第2期15卷 219-221页

作者： Jian Chen Huan Chen fengquan liu Zheng Qing Fu International Genome Center Jiangsu UniversityZhenjiang 212013China Institute of Plant Protection Jiangsu Academy of Agricultural SciencesNanjingJiangsu 210014China Department of Biological Sciences University of South CarolinaColumbiaSC 29208USA

Plant diseases caused by a wide variety of pathogens have had tremendous negative impacts on human *** example,the oomycete pathogen Phytophthora infestans,which causes severe late blight disease on potatoes,triggered the infamous Irish Potato *** prevent such tragedies from happening again and to reduce crop losses,it is imperative to implement effective disease control and management strategies.A deeper and more comprehensive understanding of how plant pathogens cause disease is critical for developing novel and effective strate-gies for controlling plant diseases.

关键词： blight comprehensive controlling

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

阵列式柔性薄膜传感器的快速扫描实

传感器与微系统 2024年第8期43卷 102-105页

作者：刘丰铨尹锡轩郭伟东牛鑫蕊东浩胡正发广东工业大学物理与光电工程学院广东广州510006 汕头问源科技有限公司广东汕头515051 汕头广工大协同创新研究院广东汕头515041

柔性薄膜传感器由于具有极高的柔韧性和可拓展性,可生产出不同大小的面积形状,通过阵列式排布,可以设计出大尺寸、多样化与数据实时特点的集成传感器模块。但对于大尺寸阵列式柔性薄膜的数据实时采集的方法仍存在挑战,本文基于STM32单... 详细信息

柔性薄膜传感器由于具有极高的柔韧性和可拓展性,可生产出不同大小的面积形状,通过阵列式排布,可以设计出大尺寸、多样化与数据实时特点的集成传感器模块。但对于大尺寸阵列式柔性薄膜的数据实时采集的方法仍存在挑战,本文基于STM32单片机以及一块4×4(可拓展)的阵列式柔性薄膜,经STM32控制,实现阵列式快速扫描,实时快速地定位出信号来源并反馈出在该薄膜单元的压力变化值。

关键词：柔性薄膜阵列式快速扫描传感器压力应变

The Xanthomonas typeⅢeffector Xop AP prevents stomatal closure by interfering with vacuolar acidification

在线全文

学校读者我要写书评

暂无评论

Journal of Integrative Plant Biology 2022年第10期64卷 1994-2008页

作者： Longyu liu Ying Li Zhengyin Xu Huan Chen Jingyi Zhang Brittany Manion fengquan liu Lifang Zou Zheng Qing Fu Gongyou Chen State Key Laboratory of Microbial Metabolism/Shanghai Collaborative Innovation Center of Agri‐Seeds School of Agriculture and BiologyShanghai Jiao Tong UniversityShanghai 200240China Department of Biological Sciences University of South CarolinaColumbia 29208USA Institute of Plant Protection Jiangsu Academy of Agricultural SciencesNanjing 210014China

Plant stomata close rapidly in response to a rise in the plant hormone abscisic acid(ABA)or salicylic acid(SA)and after recognition of pathogenassociated molecular patterns(PAMPs).Stomatal closure is the result of vacuolar convolution,ion efflux,and changes in turgor pressure in guard *** bacteria secrete typeⅢeffectors(T3Es)that interfere with plant defense mechanisms,causing severe plant disease ***,we show that the virulence and infection of Xanthomonas oryzae ***(Xoc),which is the causal agent of rice bacterial leaf streak disease,drastically increased in transgenic rice(Oryza sativa L.)plants overexpressing the Xoc T3E gene Xop AP,which encodes a protein annotated as a *** discovered that Xop AP binds to phosphatidylinositol 3,5-bisphosphate(Ptd Ins(3,5)P_(2)),a memb rane phospholipid that functions in p H control in lysosomes,membrane dynamics,and protein *** AP inhibited the acidification of vacuoles by competing with vacuolar H^(+)-pyrophosphatase(V-PPase)for binding to Ptd Ins(3,5)P_(2),leading to stomatal *** rice overexpressing Xop AP also showed inhibition of stomatal closure when challenged by Xoc infection and treatment with the PAMP ***,Xop AP suppressed flg22-induced gene expression,reactive oxygen species burst and callose deposition in host plants,demonstrating that Xop AP subverts PAMP-triggered immunity during Xoc *** together,these findings demonstrate that Xop AP overcomes stomatal immunity in plants by binding to lipids.

关键词： Ptd Ins(3 5)P2 stomatal closure typeⅢeffector vacuole acidification Xanthomonas Xop AP

基于STM32的柔性传感器信号检测系统研究

在线全文

学校读者我要写书评

暂无评论

现代信息科技 2024年第12期8卷 178-182,187页

作者：杨焜宇郭伟东尹锡轩刘丰铨胡正发广东工业大学物理与光电工程学院广东广州510006 汕头问源科技有限公司广东汕头515000 汕头广工大协同创新研究院广东汕头515000

针对柔性传感器的信号采集检测需求,基于STM32单片机和4×4阵列型柔性压力传感器,研究一种以单片机为核心单元、面向柔性传感器压力定位、压力变化、压力定量的实时数据采集检测系统。该检测系统采用单片机嵌入式程序结合快速行列扫描... 详细信息

针对柔性传感器的信号采集检测需求,基于STM32单片机和4×4阵列型柔性压力传感器,研究一种以单片机为核心单元、面向柔性传感器压力定位、压力变化、压力定量的实时数据采集检测系统。该检测系统采用单片机嵌入式程序结合快速行列扫描技术对采集的电信号数据进行分析、转换及分类显示,实现了精准的压力感知定位、分辨与测定,研究结果将会极大地推进融合计算机扫描技术和柔性阵列传感器新应用的推出。

关键词：柔性压力传感器快速扫描 STM32单片机信号检测

Mechanism of β-1,3-glucanase cooperating with HSAF in Lysobacter enzymogenes to Antagonize Pythium aphanidermatum

在线全文

学校读者我要写书评

暂无评论

Mechanism of β-1,3-glucanase cooperating with HSAF in Lysob...

Evaluation of suitable reference genes for normalization of quantitative real-time PCR analysis in rice plants under Xanthomonas oryzae ***--infection and melatonin supplementation

中国植物病理学会2023年学术年会

作者： liu Haofei XU Gaoge liu fengquan College of Plant Protection Nanjing Agricultural University Institute of Plant Protection Jiangsu Academy of Agricultural SciencesJiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base of Ministry of Science and Technology

Plant diseases cause serious crop *** is one of the effective measures to control plant diseases caused by *** enzymogenes is an environmentally ubiquitous bacteria against a variety of pathogenic fungi and *** antagonistic ability mainly due to the production of secondary metabolite HSAF and extracellular lytic enzymes such as β-1,*** this study,we found that when incubating *** OH 11 with the pathogen Pythium aphanidermatum,the production of HSAF significantly *** same results were obtained when incubating with β-1,3-glucan,which is one of the components of the cell wall of *** study showed that *** OH 11 used four β-1,3-glucanases(GluA,GluB,GluC,and endoglucanase) to degrade β-1,3-glucan into glucose and then provided energy for itself,thus increasing the production of *** this process,GluB was the last step to degrad β-1,3-glucan into *** also found that when *** OH 11 came into contact with ***,there was a correlation between the production of β-1,3-glucanase and *** β-1,3-glucan in the cell wall of *** stimulated the secretion of β-1,3-glucanase in *** OH 11,which in turn degraded β-1,3-glucan into glucose,providing more energy for *** OH 11 to synthesize more HSAF and effectively eliminate *** above results indicated that when *** encounters oomycetes,it will preferentially use β-1,3-glucanase as a weapon to degrade the cell wall of oomycetes to gain more energy,thus launching its ultimate weapon HSAF to kill oomycetes.

关键词：

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

食物生产加工与营养(英文) 2020年第1期2卷 207-216页

作者： Xian Chen Pedro Laborda Yan Dong fengquan liu Institute of Plant Protection Jiangsu Key Laboratory for Food Quality and Safety–State Key Laboratory Cultivation Base of Ministry of Science and TechnologyJiangsu Academy of Agricultural SciencesNanjing 210014PR China State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products Zhejiang Academy of Agricultural SciencesHangzhou 310021PR China School of Life Sciences Nantong UniversityNantong 226019PR China

Exogenous melatonin(MT)was found to be an interesting tool for enhancing the resistance of rice to ***(Xoo)-caused bacterial blight(BB).However,the accurate comparison of the expression levels across samples was a challenging *** this work,the stability of 10 common used housekeeping genes under Xoo-infection and MT supplementation in rice was analyzed using quantitative real-time PCR(qRT-PCR),and algorithms geNorm,NormFinder and *** results indicated that most reference genes remained stable in Xoo-infected rice plants,while a number of reference genes were affected by MT *** all studied genes,the transcript levels of 18S(18S ribosomal RNA)and UBC(Ubiquitin-conjugating enzyme E2)remained unaltered by Xoo infection,while UBC and UBQ5(Ubiquitin 5)were the most stable genes when examining simultaneous Xoo-infection and MT supplementation,demonstrating that UBC is a suitable reference gene for qRT-PCR data normalization in rice under Xoo-infection and MT supplementation.

关键词： Rice Xanthomonas oryzae pv.oryzae Melatonin qRT-PCR Reference genes