检索结果-南通市图书馆

维普期刊数据库博看期刊

Ultra-stable super-resolution fluorescence cryo-microscopy for correlative light and electron cryo-microscopy

在线全文

学校读者我要写书评

暂无评论

Science China(Life Sciences) 2018年第11期61卷 1312-1319页

作者： Xiaojun Xu Yanhong Xue Buyun Tian fengping feng Lusheng Gu Weixing Li Wei Ji Tao Xu College of Life Science and Technology Huazhong University of Science and TechnologyWuhan 430074China National Laboratoty of Biomacromolecules CAS Center for Excellence in BiomacromoleculesInstitute of BiophysicsChinese Academy of SciencesBeijing 100101China Center for Biological Instrument Development Core Facility for Protein ResearchInstitute of BiophysicsChinese Academy of SciencesBeijing 100101China College of Life Science University of Chinese Academy of SciencesBeijing 100049 China

Remarkable progress in correlative light and electron cryo-microscopy(cryo-CLEM) has been made in the past decade. A crucial component for cryo-CLEM is a dedicated cryo-fluorescence microscope(cryo-FM). Here, we describe an ultra-stable superresolution cryo-FM that exhibits excellent thermal and mechanical stability. The temperature fluctuations in 10 h are less than0.06 K, and the mechanical drift over 5 h is less than 200 nm in three dimensions. We have demonstrated the super-resolution imaging capability of this system(average single molecule localization accuracy of ～13.0 nm). The results suggest that our system is particularly suitable for long-term observations, such as single molecule localization microscopy(SMLM) and cryogenic super-resolution correlative light and electron microscopy(csCLEM).

关键词： cryogenic cryostat super-resolution Cryo-FM Cryo-PALM csCLEM

Cryogenic superresolution correlative light and electron microscopy of vitreous sections

在线全文

学校读者我要写书评

暂无评论

Biophysics Reports 2022年第4期8卷 193-204页

作者： Buyun Tian Maoge Zhou fengping feng Xiaojun Xu Pei Wang Huiqin Luan Wei Ji Yanhong Xue Tao Xu National Laboratory of Biomacromolecules CAS Center for Excellence in Biomacromolecules Institute of Biophysics Chinese Academy of Sciences University of Chinese Academy of Sciences National Research Center for Rehabilitation Technical Aids

Fluorescence microscopy and electron microscopy complement each other as the former provides labelling and localisation of specific molecules and target structures while the latter possesses excellent revolving power of fine structure in context. These two techniques can combine as correlative light and electron microscopy(CLEM) to reveal the organisation of materials within the cell. Frozen hydrated sections allow microscopic observations of cellular components in situ in a near-native state and are compatible with superresolution fluorescence microscopy and electron tomography if sufficient hardware and software support is available and a well-designed protocol is followed. The development of superresolution fluorescence microscopy greatly increases the precision of fluorescence annotation of electron tomograms. Here, we provide detailed instructions on how to perform cryogenic superresolution CLEM on vitreous sections. From fluorescence-labelled cells to high pressure freezing, cryoultramicrotomy, cryogenic single-molecule localisation microscopy, cryogenic electron tomography and image registration, electron tomograms with features of interest highlighted by superresolution fluorescence signals are expected to be obtained.

关键词： Cryogenic Superresolution Fluorescence microscopy Electron microscopy Correlative light and electron microscopy (CLEM) Electron tomography

A unified deep-learning network to accurately segment insulin granules of different animal models imaged under different electron microscopy methodologies

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Protein & Cell 2019年第4期10卷 306-311页

作者： Xiaoya Zhang Xiaohong Peng Chengsheng Han Wenzhen Zhu Lisi Wei Yulin Zhang Yi Wang Xiuqin Zhang Hao Tang Jianshe Zhang Xiaojun Xu fengping feng Yanhong Xue Erlin Yao Guangming Tan Tao Xu Liangyi Chen State Key Laboratory of Membrane Biology Beijing Key Laboratory of Cardiometabolic Molecular Medicine Institute of Molecular Medicine Peking University Beijing 100871 China. Drug Discovery Center Key Laboratory of Chemical Genomics Peking University Shenzhen Graduate School Shenzhen 518055 China. University of Chinese Academy of Sciences Beijing 100049 China. Marine Science College of Zhejiang Ocean University National Engineering Research Center of Marine Facilities Aquaculture Zhoushan 316022 China. National Laboratory of Biomacromolecules CAS Center for Excellence in Biomacromolecules Institute of Biophysics Chinese Academy of Sciences Beijing 100101 China. National Laboratory of Biomacromolecules CAS Center for Excellence in Biomacromolecules Institute of Biophysics Chinese Academy of Sciences Beijing 100101 China. xueyanhong@***. University of Chinese Academy of Sciences Beijing 100049 China. yaoerlin@***.

Insulin is important for body metabolism regulation and glucose homeostasis,and its dysregulation often leads to metabolic syndrome(MS)and *** is normally stored in large dense-core vesicles(LDCVs)in pancreatic beta cells,and significant reductions in the number,size,gray level and density of insulin granules confer diabetes both in mice(Xue et al.,2012)and humans(Masini et al.,2012).

关键词： deep-learning network animal

在线全文

学校读者我要写书评

暂无评论

创伤性脑损伤促进骨折愈合机制研究进展

中国修复重建外科杂志 2024年第1期38卷 125-132页

作者：李怀任韩凤平孟静常文利冯立济宁医学院临床医学院山东济宁272000 济宁市第一人民医院急诊创伤外科山东济宁272000

目的总结创伤性脑损伤(traumatic brain injury,TBI)促进骨折愈合相关机制的研究进展,为临床治疗骨折不愈合提供理论依据。方法查阅国内外关于TBI促进骨折愈合的研究文献,从神经、体液及免疫3个方面总结TBI在骨折愈合中的作用,探讨骨折... 详细信息

目的总结创伤性脑损伤(traumatic brain injury,TBI)促进骨折愈合相关机制的研究进展,为临床治疗骨折不愈合提供理论依据。方法查阅国内外关于TBI促进骨折愈合的研究文献,从神经、体液及免疫3个方面总结TBI在骨折愈合中的作用,探讨骨折不愈合治疗新思路。结果大量研究表明,骨折合并TBI患者的骨折愈合速度较单纯骨折患者更快。骨折合并TBI患者体液中各种细胞因子、激素的表达量明显高于单纯骨折患者;神经系统释放的神经因子透过受损血脑屏障到达骨折部位,同时合并TBI的骨折端炎症细胞和炎症因子的趋化、聚集与单纯骨折患者也有显著差异。复杂的体液、神经及免疫调节网络共同促进骨折端血管再生、成骨细胞分化并抑制破骨细胞活性。结论TBI通过复杂的神经、体液及免疫调节网络促进骨折愈合,提示通过干预骨折周围微环境可以治疗骨折不愈合。

关键词：创伤性脑损伤骨折愈合细胞因子激素神经因子炎症

在线全文

学校读者我要写书评

暂无评论

生物材料在Masquelet技术中的应用

中国组织工程研究 2024年第10期28卷 1634-1640页

作者：韩凤平李怀任常文利田宝方冯立济宁医学院山东省济宁市272000 济宁市第一人民医院山东省济宁市272000

背景:骨缺损的修复和治疗难度较高,其临床治疗方案多样,Masquelet技术是一种成功率高、疗效可靠且已经应用于临床的治疗方法,但是该技术目前机制并未完全明确,且该技术在临床应用上还存在一些问题,技术仍然不是特别成熟。目的:对现阶段... 详细信息

背景:骨缺损的修复和治疗难度较高,其临床治疗方案多样,Masquelet技术是一种成功率高、疗效可靠且已经应用于临床的治疗方法,但是该技术目前机制并未完全明确,且该技术在临床应用上还存在一些问题,技术仍然不是特别成熟。目的:对现阶段在Masquelet技术改进的研究中所应用的生物材料进行整理与归纳,为该技术的进一步发展提供思路与参考。方法:通过计算机在中国知网和PubMed数据库中检索2013年1月至2022年11月的相关文献,中文检索词为“Masquelet技术,膜诱导技术,诱导膜,生物材料,骨缺损”;英文检索词为“Masquelet technique,Induced membrane technique,Induced membrane,biomaterial,Bone defect”,对最终纳入符合标准的58篇文献进行综述。结果与结论:①Masquelet技术的出现与不断发展为治疗骨缺损提供了一种治疗策略,有些研究者将研究方向放在研究出更好的间隔材料、自体骨的替代材料和与诱导膜具有相同性能的膜材料上,以便于将两阶段术式简化,缩短治疗时间和减轻患者的痛苦。②硫酸钙、硅胶、聚乳酸-羟基乙酸共聚物和聚丙烯在动物实验或者临床应用中可以替代聚甲基丙烯酸甲酯骨水泥形成诱导膜,且各有优势,但是与预想不同,常见的钛和聚乙烯醇海绵反而不能在这一步中作为聚甲基丙烯酸甲酯骨水泥的替代材料。③自体骨的替代材料多种多样,同种异体骨、β-磷酸三钙、可吸收性明胶海绵、α-硫酸钙半水合物、生物活性玻璃、钛和钽等材料都在临床或基础实验中证明了其可以在术式第二阶段中减少自体松质骨植骨量的能力,其中同种异体骨、β-磷酸三钙、生物活性玻璃、钛和钽等材料可替代自体骨作为移植物,其余材料需与自体骨混合使用。④仿生诱导膜、人羊膜、人脱细胞真皮、聚四氟乙烯甚至自体皮质骨通过实验证实了其与诱导膜具有相似性能。⑤现阶段,众多生物材料在该技术中的应用与研究大多数仍存在于基础研究阶段,尚未应用于临床实践或大范围推广,但是上述材料可为Masquelet技术机制的探究、术式的改进与临床应用提供更充分的理论依据和新思路。

关键词： Masquelet技术膜诱导技术诱导膜生物材料骨再生骨缺损骨愈合骨不愈合骨折

Core Metabolic Features and Hot Origin of Bathyarchaeota

在线全文

学校读者我要写书评

暂无评论

Engineering 2019年第3期5卷 498-504页

作者： Xiaoyuan feng Yinzhao Wang Rahul Zubin fengping Wang State Key Laboratory of Microbial Metabolism School of Life Sciences and Biotechnology Shanghai Jiao Tong University Shanghai 200240 China State Key Laboratory of Ocean Engineering Shanghai Jiao Tong University Shanghai 200240 China

The archaeal phylum Bathyarchaeota comprises highly diversified subgroups and is considered to be one of the most abundant microorganisms on earth. The metabolic features and evolution of this phylum still remain largely unknown. In this article, a comparative metabolic analysis of 15 newly reconstructed and 36 published metagenomic assembled genomes (MAGs) spanning 10 subgroups was performed, revealing the core metabolic features of Bathyarchaeota—namely, protein, lipid, and benzoate degradation;glycolysis;and the Wood–Ljungdahl (WL) pathway, indicating an acetyl-CoA-centralized metabolism within this phylum. Furthermore, a partial tricarboxylic acid (TCA) cycle, acetogenesis, and sulfur-related metabolic pathways were found in specific subgroups, suggesting versatile metabolic capabilities and ecological functions of different subgroups. Intriguingly, most of the MAGs from the Bathy-21 and -22 subgroups, which are placed at the phylogenetic root of all bathyarchaeotal lineages and likely represent the ancient Bathyarchaeota types, were found in hydrothermal environments and encoded reverse gyrase, suggesting a hyperthermophilic feature. This work reveals the core metabolic features of Bathyarchaeota, and indicates a hot origin of this archaeal phylum.

关键词： Bathyarchaeota Metagenomics Comparative genomics Hyperthermophilic adaptation

可重构解耦并联移动机器人构型综合及性能分析

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

机械传动 2024年第3期48卷 45-51页

作者：潘武星李瑞琴宁峰平李艳龙丰晓东中北大学机械工程学院山西太原030051

为提高机器人承载能力,减少驱动电动机能量消耗且使轮腿模式切换平稳高效,提出了一种可重构解耦并联移动机器人构型,并对其进行了构型综合研究及解耦性和奇异性分析。根据机器人机械腿期望自由度,综合出4种主运动支链;基于螺旋理论的去... 详细信息

为提高机器人承载能力,减少驱动电动机能量消耗且使轮腿模式切换平稳高效,提出了一种可重构解耦并联移动机器人构型,并对其进行了构型综合研究及解耦性和奇异性分析。根据机器人机械腿期望自由度,综合出4种主运动支链;基于螺旋理论的去约束法,综合出58种约束支链;基于螺旋理论的虚拟链法,综合出3R球面并联机构作为机械腿承载支链。随后,给出两个机器人实例。以其中一个机器人为例,建立输入-输出方程,分析了机械腿解耦性;通过反证法,验证了可重构单元的奇异位形,并基于Grassmann线几何判断出奇异类型。最后,分析了承载支链的奇异性对于机械腿运动输出的影响。结果说明,机器人机械腿采用并联支链构型,具有较强的承载能力;机器人基于约束奇异方式实现轮腿切换,可使得切换动作更加平稳;机械腿的可重构性可使得驱动电动机能量消耗减少;机械腿各驱动相互解耦,降低了控制难度,使得机械腿轮腿模式切换更加高效。

关键词：并联移动机器人构型综合螺旋理论可重构性解耦性

Advances in the Immunotherapeutic Potential of Isocitrate Dehydrogenase Mutations in Glioma

在线全文

学校读者我要写书评

暂无评论

Neuroscience Bulletin 2022年第9期38卷 1069-1084页

作者： feng Tang Zhiyong Pan Yi Wang Tian Lan Mengyue Wang fengping Li Wei Quan Zhenyuan Liu Zefen Wang Zhiqiang Li Brain Glioma Center and Department of Neurosurgery Wuhan University Zhongnan HospitalWuhan430071China Department of Physiology Wuhan University School of Basic Medical SciencesWuhan430071China

Isocitrate dehydrogenase(IDH)is an essential metabolic enzyme in the tricarboxylic acid cycle(TAC).The high mutation frequency of the IDH gene plays a complicated role in *** addition to affecting gliomas directly,mutations in IDH can also alter their immune microenvironment and can change immune-cell function in direct and indirect *** mutations mediate immune-cell infiltration and function by modulating immune-checkpoint gene expression and chemokine *** addition,IDH mutation-derived D2-hydroxyglutarate can be absorbed by surrounding immune cells,also affecting their *** this review,we summarize current knowledge about the effects of IDH mutations as well as other gene mutations on the immune microenvironment of *** also describe recent preclinical and clinical data related to IDH-mutant inhibitors for the treatment of ***,we discuss different types of immunotherapy and the immunotherapeutic potential of IDH mutations in gliomas.

关键词： IDH mutation Tumor immune microenvironment Immunotherapy Glioma

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

精确化磨矿对钨矿粒度分布特性的影响

有色金属（选矿部分） 2024年第3期 76-82页

作者：李雨晴谢峰徐凤平万林海戴修湖吴彩斌江西理工大学资源与环境工程学院江西赣州341000 江西省修水香炉山钨业有限公司江西九江332000 战略金属矿产资源低碳加工与利用江西省重点实验室江西赣州341000

为探明精确化磨矿技术对工业磨矿产品粒度特性的影响,通过实验室对比试验发现,精确化制度下的磨矿产品粒度分布更合理,-0.074 mm粒级产率由34.76%提升至44.64%,而过磨粒级与现场一段排矿基本保持一致,有效改善了钨矿石磨矿产品中钨金属... 详细信息

为探明精确化磨矿技术对工业磨矿产品粒度特性的影响,通过实验室对比试验发现,精确化制度下的磨矿产品粒度分布更合理,-0.074 mm粒级产率由34.76%提升至44.64%,而过磨粒级与现场一段排矿基本保持一致,有效改善了钨矿石磨矿产品中钨金属的分布特性,与一段溢流产品相比,精确化闭路磨矿产品中-0.15+0.010 mm粒级钨金属含量高出现场一段溢流15.93个百分点,-0.010 mm粒级钨金属含量低于现场一段溢流26.52个百分点。精确化磨矿后的浮选产品中钨粗精矿回收率比精确化磨矿前高出5.92个百分点,增幅为23.61%;尾矿产品比精确化磨矿前低出10.00个百分点,降幅为31.95%。对香炉山钨矿三选厂进行了精确化磨矿工业试验,与工业试验前相比,精确化磨矿工业应用后一段分级溢流产品中+0.15 mm粒级和-0.010 mm粒级含量分别减少了7.41%和11.25%;-0.15+0.010 mm粒级含量从66.44%提升至69.44%,增幅为4.52%;-0.010 mm粒级中钨金属含量减少了20.51%;-0.15+0.010 mm粒级中钨金属含量从79.59%提升至82.75%,增幅为3.97%。钨尾矿中-0.010 mm粒级含量减少了11.90%,-0.010 mm粒级和+0.15 mm粒级中钨金属量分别减少了30.92%和11.83%。三选厂钨金属回收率从78.65%提升至80.80%,提升了2.15个百分点,增幅2.73%。精确化磨矿试验证实了对香炉山钨矿磨矿产品粒度特性分布改善和浮选指标提升均取得显著效果。

关键词：钨矿精确化磨矿粒度分布特性浮选回收率