Ultra-stable super-resolution fluorescence cryo-microscopy for correlative light and electron cryo-microscopy

作     者：Xiaojun Xu Yanhong Xue Buyun Tian Fengping Feng Lusheng Gu Weixing Li Wei Ji Tao Xu 

作者机构：College of Life Science and TechnologyHuazhong University of Science and TechnologyWuhan 430074China National Laboratoty of BiomacromoleculesCAS Center for Excellence in BiomacromoleculesInstitute of BiophysicsChinese Academy of SciencesBeijing 100101China Center for Biological Instrument DevelopmentCore Facility for Protein ResearchInstitute of BiophysicsChinese Academy of SciencesBeijing 100101China College of Life ScienceUniversity of Chinese Academy of SciencesBeijing 100049 China 

出 版 物：《Science China(Life Sciences)》 (中国科学（生命科学英文版）)

年 卷 期：2018年第61卷第11期

页      面：1312-1319页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 09[农学] 

基　　金：supported by the National Key R&D Program of China (2016YFA0500203, 2016YFA0502400, 2017YFA0504700, 2017YFA0505300) the National Natural Science Foundation of China (31661143041, 31127901) Joint Program between Chinese Academy of Sciences and Peking University 

主　　题：cryogenic cryostat super-resolution Cryo-FM Cryo-PALM csCLEM 

摘      要：Remarkable progress in correlative light and electron cryo-microscopy(cryo-CLEM) has been made in the past decade. A crucial component for cryo-CLEM is a dedicated cryo-fluorescence microscope(cryo-FM). Here, we describe an ultra-stable superresolution cryo-FM that exhibits excellent thermal and mechanical stability. The temperature fluctuations in 10 h are less than0.06 K, and the mechanical drift over 5 h is less than 200 nm in three dimensions. We have demonstrated the super-resolution imaging capability of this system(average single molecule localization accuracy of ～13.0 nm). The results suggest that our system is particularly suitable for long-term observations, such as single molecule localization microscopy(SMLM) and cryogenic super-resolution correlative light and electron microscopy(csCLEM).