检索结果-南通市图书馆

Journal of Oceanology and Limnology 2023年第1期41卷 352-363页

作者： fengjuan jiang Qingyao WANG Jingjing DU Fu LÜ Qing NIE Weihong ZHAO College of Marine and Biological Engineering Yancheng Institute of TechnologyYancheng 224051China

The appropriate reference gene is a prerequisite for accurate normalization of gene expression level,and research on suitable reference genes in clam Cyclina sinensis is *** improve the situation,we selected five commonly used housekeeping genes,including β-actin,Elongation factor 1-α(EF1-α),Glyceraldehyde-3-pho sphate dehydrogenase(GAPDH),40S ribosomal protein S18(RPS18),and Tubulin a(TUB-α),then evaluated their expression stability in different adult tissues and under different experimental treatments(salinity stress and Vibrio parahaemolyticus infection).Their expression stability was analyzed by three frequently used programs,geNorm,NormFinder,and *** analysis indicated that multiple genes should be used for normalization,and we concluded that the reference gene combination GAPDH-RPS18-β-actin,should be used for qRT-PCR analysis in different tissues of *** under normal physiological *** the clams under salinity stress and Vibrio infection,EF1-α-GAPDHRPS18 was recommended as the gene combination for qRT-PCR ***-αwas generally poorly ranked by all programs,and should not be used in future *** study should provide fundamental support for accurate quantitative gene expression analysis of this species.

关键词： Cyclina sinensis reference gene different tissues salinity stress Vibrio infection

Heritability of resistance-related gene expression traits and their correlation with body size of clam Meretrix petechialis

在线全文

学校读者我要写书评

暂无评论

Journal of Oceanology and Limnology 2020年第2期38卷 571-578页

作者： jiang fengjuan YUE Xin ZHANG Shujing YU Jiajia WANG Rui LIU Baozhong WANG Hongxia Key Laboratory of Experimental Marine Biology Institute of OceanologyCenter for Ocean Mega-ScienceChinese Academy of SciencesQingdao 266071China Laboratory for Marine Biology and Biotechnology Qingdao National Laboratory for Marine Science and TechnologyQingdao 266000China University of Chinese Academy of Sciences Beijing 100049China

Gene expression variation can be considered as a phenotype,and it plays an important role in both acclimation and ***,genetic variation of gene expression received much less attention than traditional commercial traits in *** estimate the genetic variation and heritability of gene transcription in clam Meretrix petechialis,five Vibrio resistance-related genes were selected for gene expression analysis in the digestive gland,and an animal linear model was used to analyze data from quantitative real-time PCR(qRT-PCR).Among the five genes,BIRC7 showed significant additive genetic variations,the heritability of this gene of 12-month-and 15-month-old clams were 0.84±0.32 and 0.91±0.34,*** heritability of other four genes(Bax,NFIL3,Big-Def,and CTL9)expression were low-tomoderate but not significantly ***,no significant phenotypic and genetic correlations between the BIRC7 transcription trait and body size were *** study highlights that certain gene expression variation is heritable and provides a reference for indirect selection of *** with high Vibrio resistance.

关键词： Meretrix petechialis transcription trait body size heritability genetic correlation

Big Data-Based Evaluation of Higher Education:Model Construction and Practice Path

在线全文

学校读者我要写书评

暂无评论

数字教育前沿（英文） 2024年第2期1卷 171-177页

作者： Shunping Wei Wenting Hou fengjuan jiang School of Education Minzu University of ChinaBeijing 100081China

After the Overall Plan for Deepening the Reform of Education Evaluation in the New Era has been released for over two years,the reform of education evaluation has achieved a good start and important phased *** the digital transformation of education evaluation and developing Big Data-based education evaluation are the main measures of current evaluation *** on the case study of the Minzu University of China,this paper systematically sorts out the relevant research,constructs the factor model and process model of Big Data-based education evaluation from the perspectives of factors and process of evaluation,puts forward the application idea of Big Data-based education evaluation from the perspectives of full business,full process and full factors,and puts forward the practical path of Big Data-based education evaluation from the aspects of application traction,teacher training and safe operation.

关键词： education evaluation Big Data evaluation model evaluation system practical path

Complement C3a activates osteoclasts by regulating the PI3K/PDK1/SGK3 pathway in patients with multiple myeloma

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Cancer Biology & Medicine 2021年第3期18卷 721-733页

作者： fengjuan jiang Hui Liu Fengping Peng Zhaoyun Liu Kai Ding Jia Song Lijuan Li Jin Chen Qing Shao Siyang Yan Kim De Veirman Karin Vanderkerken Rong Fu Department of Hematology Tianjin Medical University General HospitalTianjin 300052China Department of Hematology and Immunology-Myeloma Center Brussels Vrije Universiteit BrusselBrussels 1090Belgium

Objective:Myeloma bone disease(MBD)is the most common complication of multiple myeloma(MM).Our previous study showed that the serum levels of C3/C4 in MM patients were significantly positively correlated with the severity of bone ***,the mechanism of C3 a/C4 a in osteoclasts MM patients remains ***:The formation and function of osteoclasts were analyzed after adding C3 a/C4 a in ***-seq analysis was used to screen the potential pathways affecting osteoclasts,and the results were verified by Western blot,q RT-PCR,and pathway ***:The osteoclast area per view induced by 1μg/m L(mean±SD:50.828±12.984%)and 10μg/m L(53.663±12.685%)of C3 a was significantly increased compared to the control group(0μg/m L)(34.635±8.916%)(P<0.001 and P<0.001,respectively).The relative m RNA expressions of genes,OSCAR/TRAP/RANKL/cathepsin K,induced by 1μg/m L(median:5.041,3.726,1.638,and 4.752,respectively)and 10μg/m L(median:5.140,3.702,2.250,and 5.172,respectively)of C3 a was significantly increased compared to the control group(median:3.137,2.004,0.573,and 2.257,respectively)(1μg/m L P=0.001,P=0.003,P<0.001,and P=0.008,respectively;10μg/m L:P<0.001,P=0.019,P<0.001,and P=0.002,respectively).The absorption areas of the osteoclast resorption pits per view induced by 1μg/m L(mean±SD:51.464±11.983%)and 10μg/m L(50.219±12.067%)of C3 a was also significantly increased(33.845±8.331%)(P<0.001 and P<0.001,respectively)compared to the *** was no difference between the C4 a and control ***-seq analysis showed that C3 a promoted the proliferation of osteoclasts using the phosphoinositide 3-kinase(PI3 K)signaling *** relative expressions of PIK3 CA/phosphoinositide dependent kinase-1(PDK1)/serum and glucocorticoid inducible protein kinases(SGK3)genes and PI3 K/PDK1/p-SGK3 protein in the C3 a group were significantly higher than in the control *** activation role of C3 a in osteoclasts of MM patients was reduced by the SGK inhibi

关键词： Multiple myeloma complement C3a osteoclasts PI3K/PDK1/SGK3 pathways SGK inhibitor

混合式教学环境中大学生学习的行为意愿影响因素研究

在线全文

学校读者我要写书评

暂无评论

电化教育研究 2021年第6期42卷 105-112,128页

作者：江凤娟中央民族大学教育学院北京100081

在经历新冠肺炎疫情期间在线教学洗礼之后,混合式教学成为高校的常见教学方式。为了更好地分析大学生在混合式教学环境中的学习的行为意愿,通过问卷收集了1346名大学生关于混合式教学环境中学习的行为意愿的调查数据,基于UTAUT扩展模型... 详细信息

在经历新冠肺炎疫情期间在线教学洗礼之后,混合式教学成为高校的常见教学方式。为了更好地分析大学生在混合式教学环境中的学习的行为意愿,通过问卷收集了1346名大学生关于混合式教学环境中学习的行为意愿的调查数据,基于UTAUT扩展模型,采用结构方程方法分析了影响大学生混合式教学环境中学习的行为意愿的关键因素及变量之间的关系。结果表明:在混合式教学环境中,移动自我效能感、绩效期望、努力期望、社会影响、成就动机对大学生学习的行为意愿有显著影响;学习风格对学习的行为意愿影响不显著;移动自我效能感在绩效期望、努力期望和学习的行为意愿之间发挥着完全中介效应,绩效期望对移动自我效能感产生积极的促进作用,而努力期望则呈现出显著的消极影响。

关键词：混合式学习行为意愿 UTAUT 大学生移动自我效能感成就动机

应对老龄化挑战的教育创新:美国高等教育代际服务学习实践

在线全文

学校读者我要写书评

暂无评论

比较教育研究 2024年第6期46卷 73-83页

作者：江凤娟中央民族大学教育学院北京100081

老龄化是全球人口结构的发展趋势,高等教育在积极应对老龄化挑战中扮演关键角色。美国多所高校通过代际服务学习模式将学生专业发展与社区需求紧密结合,为高等教育积极参与老年教育和社会服务积累了丰富实践经验。代际服务学习强调提高... 详细信息

老龄化是全球人口结构的发展趋势,高等教育在积极应对老龄化挑战中扮演关键角色。美国多所高校通过代际服务学习模式将学生专业发展与社区需求紧密结合,为高等教育积极参与老年教育和社会服务积累了丰富实践经验。代际服务学习强调提高公民责任感,注重学生和被服务对象的共同受益。准备阶段充分考虑学生专业发展目标和实际老年服务需求;行动阶段注重在提升老年服务质量的同时,促进学生专业知识的理解和实践;反思阶段要求学生记录过程性资料,并进行批判性思考;开展多主体的评估和反馈。美国高等教育代际服务学习彰显了尊重老年人和学生双方需求的互惠互助价值观,通过基于证据的循证式评估机制,打破传统教育的角色模糊策略,呈现出专业学习和社会服务并重的鲜明特色。

关键词：美国高等教育代际服务学习社会服务积极老龄化

NKT细胞的生物特性及其在多发性骨髓瘤中应用的研究进展

在线全文

学校读者我要写书评

暂无评论

中国肿瘤临床 2018年第17期45卷 904-907页

作者：姜凤娟付蓉天津医科大学总医院血液科天津市300052

NKT细胞是一种特殊的T淋巴细胞亚群,其表面既有T细胞受体,又有NK细胞受体。NKT细胞在肿瘤、自身免疫性疾病和微生物感染中起到调节免疫应答的作用,是联系固有免疫和获得性免疫的"桥梁"之一。多发性骨髓瘤(multiple myeloma,MM)是一种浆... 详细信息

NKT细胞是一种特殊的T淋巴细胞亚群,其表面既有T细胞受体,又有NK细胞受体。NKT细胞在肿瘤、自身免疫性疾病和微生物感染中起到调节免疫应答的作用,是联系固有免疫和获得性免疫的"桥梁"之一。多发性骨髓瘤(multiple myeloma,MM)是一种浆细胞异常增殖的恶性血液系统疾病。MM细胞表达CD1d分子并且可被具有CD1d限制性的NKT细胞杀伤。近些年,NKT细胞的抗MM作用成为研究热点,本文就NKT细胞生物特性及其在MM治疗中的应用进行综述。

关键词： NKT细胞多发性骨髓瘤应用治疗

基层官员政策再制定:规则的冲突——以中小学布局调整政策执行为例

在线全文

学校读者我要写书评

暂无评论

教育学术月刊 2013年第2期 78-83页

作者：江凤娟中央民族大学教育学院

一个地区教育在发展过程中形成的内部规则和作为国家制定的行政性法令式外部规则往往产生冲突。基层官员有着政府官员和群众利益代表的双重身份,在执行政策过程中必然要处理这种冲突,对政策价值、政策目标、政策标准等通过各种方式对政... 详细信息

一个地区教育在发展过程中形成的内部规则和作为国家制定的行政性法令式外部规则往往产生冲突。基层官员有着政府官员和群众利益代表的双重身份,在执行政策过程中必然要处理这种冲突,对政策价值、政策目标、政策标准等通过各种方式对政策进行解释、调整或修改的个体行动,也就出现了政策再制定的必然现象。而这种政策再制定往往造成对教育系统甚至社会秩序的破坏。

关键词：基层官员政策再制定内部规则外部规则

基层官员的政策目标再制定——以中小学布局调整政策为例

在线全文

学校读者我要写书评

暂无评论

教育学术月刊 2012年第8期 58-62页

作者：江凤娟中央民族大学教育学院

从政策执行个体的视角分析,政策执行是一种演进的行为模式为特征的个体行动。政策执行过程中基层官员会根据实际情况和自身的理解重新"制定政策",其中最直接的体现是政策目标。通过对开和县中小学布局调整政策执行的调查分析,研究将基... 详细信息

从政策执行个体的视角分析,政策执行是一种演进的行为模式为特征的个体行动。政策执行过程中基层官员会根据实际情况和自身的理解重新"制定政策",其中最直接的体现是政策目标。通过对开和县中小学布局调整政策执行的调查分析,研究将基层官员政策目标再制定大致分为三种类型:简化目标、量化目标和附加目标。

关键词：基层官员政策目标再制定政策执行

在线全文

学校读者我要写书评

暂无评论

互联网红利与我国人力资源跨越式发展

教育研究 2018年第12期39卷 118-126页

作者：江凤娟吴峰中央民族大学教育学院北京大学教育学院

互联网的出现重构了新的学习时空观,为我国人力资源发展带来了双重红利。互联网先后给我国人力资源发展带来了三种新模式,分别是"互联网+学历"模式的国家远程教育计划、"互联网+能力"模式的企业数字化学习工程、"互联网+知识"模式的大... 详细信息

互联网的出现重构了新的学习时空观,为我国人力资源发展带来了双重红利。互联网先后给我国人力资源发展带来了三种新模式,分别是"互联网+学历"模式的国家远程教育计划、"互联网+能力"模式的企业数字化学习工程、"互联网+知识"模式的大学全球化慕课运动,它们快速地推动了我国人力资源发展。研究发现,解决社会对于远程教育高层次学历需求问题、解决广大中小企业对于数字化学习的需求与零供应的矛盾问题、解决大规模公开在线课程中的流失陷阱问题,互联网红利将得到巨大地深度释放。破解这些矛盾,需要制定"互联网+人力资源发展"的顶层设计;举办远程研究生教育,满足高层次的学历学习需求;实施国家数字化学习工程,填补中小企业职工能力提升的学习空白;建立学分银行制度,搭建学习立交桥;推动我国终身学习立法,从制度层面保障我国人力资源发展。

关键词：互联网红利人力资源数字化学习跨越式