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Dietary supplementation with N-carbamoylglutamate initiated from the prepartum stage improves lactation performance of postpartum dairy cows

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Animal Nutrition 2021年第1期7卷 232-238页

作者： fengfei gu Chao Miao Luyi Jiang Diming Wang Hongyun Liu Jianxin Liu Institute of Dairy Science MoE Key Laboratory of Molecular Animal NutritionCollege of Animal SciencesZhejiang UniversityHangzhou310058China

The objective of this study was to investigate the effects of supplementing N-carbamoylglutamate(NCG),an Arg enhancer,on amino acid(AA)supply and utilization and productive performance of early-lactating dairy *** multiparous Chinese Holstein dairy cows were randomly divided into con-trol(CON,n=15)and NCG(CON diet supplemented with NCG at 20 g/d per cow,n=15)groups at 4 wk before *** were offered individually in tie-stalls,and NCG was supplemented by top-dress feeding onto total mixed ration for the NCG *** experiment lasted until wk 10 after *** matter intake tended to be higher(P=0.06),and yields of milk(P<0.01),milk protein(P<0.01),and milk fat(P<0.01)were higher in the NCG-cows than in the *** activities of aspartate aminotransferase(P<0.01),alanine aminotransferase(P=0.03),and plasma level ofβ-hydroxybutyrate(P=0.04)were lower in the NCG-cows than in the CON-cows,whereas plasma glucose(P=0.05)and nitric oxide(NO,P<0.01)concentrations were *** vein concentrations of Cys(P<0.01),Pro(P<0.01),Tyr(P=0.05),most essential AA except Thr and His(P<0.01),total essential AA(P<0.01),and total AA(P<0.01)were higher in the NCG-cows than in the *** arterial supply of all AA was greater in the NCG-cows than in the *** NCG-cows had higher mammary plasma flow of AA(P=0.04)and clearance rate of Cys(P<0.01),Pro(P<0.01)and Asp(P<0.01),and higher ratios of uptake to output of Met(P=0.05),Lys(P<0.01),Cys(P=0.01),Pro(P=0.03),and Asp(P=0.01).In summary,addition of NCG initiated from the prepartum period improved the lactation performance of postpartum dairy cows,which might attribute to greater Arg and NO concentrations,as well as improved AA supply and utilization,liver function,and feed intake in these cows.

关键词： N-carbamoylglutamate Arginine Transition cow Lactation performance

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Supplementing N-carbamoylglutamate in late gestation increases newborn calf weight by enhanced placental expression of mTOR and angiogenesis factor genes in dairy cows

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Animal Nutrition 2021年第4期7卷 981-988页

作者： fengfei gu Luyi Jiang Linyu Xie Diming Wang Fengqi Zhao Jianxin Liu Institute of Dairy Science College of Animal SciencesZhejiang UniversityHangzhou310058China Department of Animal and Veterinary Sciences University of VermontBurlingtonVT 05405USA

The objective of this study was to investigate whether supplementation with N-carbamoylglutamate(NCG)to cows during late gestation alters uteroplacental tissue nutrient transporters,calf metabolism and newborn *** multiparous Chinese Holstein cows were used in a randomized complete block design *** the last 28 d of pregnancy,cows were fed a diet without(CON)or with NCG(20 g/d per cow).The body weight of calves was weighed immediately after *** samples were collected at parturition and used to assess mRNA expression of genes involved in transport of arginine,glucose,fatty acid and angiogenesis factors,as well as the mammalian target of rapamycin(mTOR)*** samples of calves before colostrum consumption were also collected for the detection of plasma parameters,amino acids(AA)and metabolomics *** newborn weight(P=0.02)and plasma Arg concentration of NCG-calves was significantly higher(P=0.05)than that of CON-calves,and the plasma concentrations of urea nitrogen tended to be lower(P=0.10)in the NCG *** mRNA abundance of genes involved in glucose transport(solute carrier family 2 member 3[SLC2 A3],Pgulated in the placentome of NCG-supplemented *** addition,17 metabolites were significantly different in the placentome of NCG-supplemented cows compared to non-supplemented cows,and these metabolites are mainly involved in arginine and proline metabolism,alanine,aspartate and glutamate metabolism,and citrate *** summary,the increased body weight of newborn calves from the NCG supplemented dairy cows maybe attributed to the increased angiogenesis and uteroplacental nutrient transport and to the activated mTOR signal pathway,which may result in the increa

关键词： N-Carbamoylglutamate Metabolomics Newborn calf weight Nutrient transporter Placentome Transition dairy cows

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Blood neutrophil extracellular traps:a novel target for the assessment of mammary health in transition dairy cows

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Journal of Animal Science and Biotechnology 2023年第3期14卷 1068-1079页

作者： Luyi Jiang Huizeng Sun fengfei gu Jin He Fengqi Zhao Jianxin Liu Institute of Dairy Science College of Animal SciencesZhejiang UniversityHangzhouChina Ministry of Education Key Laboratory of Molecular Animal Nutrition Zhejiang UniversityHangzhouChina Institute of Animal Genetics and Reproduction College of Animal SciencesZhejiang UniversityHangzhouChina Department of Animal&Veterinary Sciences University of VermontBurlingtonUSA

Background:Mammary health is important for transition dairy cows and has been well recognized to exert decisive effects on animal ***,the factors influencing mammary health are still *** somatic cell count(DSCC)could reflect the mastitis risk since it is the percentage of neutrophils plus lymphocytes in total somatic cells and could be reflective of mammary health of dairy *** work aimed to investigate the assessment and prognosis of the health of transition cows based on blood neutrophil extracellular traps(NETs).Results:Eighty-four transition Holstein dairy cows were *** serum was sampled in all the animals at week 1 pre-and postpartum,and milk was sampled at week 1 *** on the DSCC in milk at week 1,cows with lower(7.4%±4.07%,n=15)and higher(83.3%±1.21%,n=15)DSCCs were *** DSCC cows had higher levels of red blood cell counts(Pguanosine(P=0.07)],higher levels of serum and milk NETs(P<0.05)and blood-milk barrier indicators,including serumβ-casein(P=0.05)and milk immunoglobulin G2(P=0.09),than those of low DSCC *** addition,lower concentrations of serum nutrient metabolites(cholesterol and albumin)(P<0.05)and a lower level of serum deoxyribonuclease I(P=0.09)were observed in high DSCC cows than in low DSCC *** the assessments performed using levels of the three prepartum serum parameters(NETs,deoxyribonuclease I andβ-casein),the area under the curve(0.973)of NETs was the *** addition,the sensitivity(1.00)and specificity(0.93)were observed for the discrimination of these cows using NETs levels with a critical value of 32.2 ng/mL(P<0.05).Conclusions:The formation of NETs in blood in transition dairy cows may damage the integrity of the blood-milk barrier and thereby increase the risk for

关键词： Blood-milk barrier Differential somatic cell count Mastitis risk Neutrophil extracellular traps

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Age-and Microbiota-Dependent Cell Stemness Plasticity Revealed by Cattle Cell Landscape

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Research 2023年第3期6卷 345-360页

作者： Jia-Jin Wu Senlin Zhu Yi-Fan Tang fengfei gu Teresa GValencak Jian-Xin Liu Hui-Zeng Sun Institute of Dairy Science College of Animal SciencesZhejiang UniversityHangzhou 310058China Ministry of Education Key laboratory of Molecular Animal Nutrition Zhejiang UniversityHangzhou 310058China Ministry of Education Innovation Team of Development and Function of Animal Digestive System Zhejiang UniversityHangzhou 310058China

Newborn ruminants are considered functionally monogastric *** poor understanding of cellular differences between newborn and mature ruminants prevents the improvement of health and performance of domestic ***,we performed the single-cell RNA sequencing on the rumen,reticulum,omasum,abomasum,duodenum,jejunum,ileum,cecum,colon,rectum,liver,salivary gland,and mammary gland from newborn and adult cattle.A comprehensive single-cell transcriptomic atlas covering 235,941 high-quality single cells and 78 cell types was deciphered.A Cattle Cell Landscape database(http://***)was established to elaborately display the data and facilitate effective annotation of cattle cell types and subtypes for the broad research *** measuring stemness states of epithelial cells in each tissue type,we revealed that the epithelial cells from newborn forestomach(rumen,reticulum,and omasum)were more transcriptionally indistinct and stochastic compared with the adult stage,which was in contrast to those of abomasum and intestinal *** rapid forestomach development during the early life of calves was driven by epithelial progenitor-like cells with high DNA repair activities and ***,in the forestomach tissues of newborn calves,the Megasphaera genus was involved in regulating the transcriptional plasticity of the epithelial progenitor-like cells by DNA methylation regulation.A novel cell type,the STOML3+cell,was found to be *** apparently plays a crucial role in stemness maintenance of its own and cholangiocytes in the hepatic *** results reveal that the age-and microbiota-dependent cell stemness plasticity drives the postnatal functional maturity of ruminants.

关键词： stomach maturity cattle

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热应激对奶牛免疫功能、消化道稳态和乳腺健康的影响及机制

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动物营养学报 2024年第9期36卷 5479-5485页

作者：吴方超顾凤飞刘建新浙江大学动物科学学院奶业科学研究所杭州310058

奶牛单位体重散热面积小,且汗腺不发达,在产奶过程中易产生大量热量,这会导致它们易遭受热应激,进而给奶牛带来多方面的不良影响,是制约奶业健康发展的重要因素之一。正常的免疫功能、消化道稳态和乳腺健康是奶牛发挥生产潜能的基本保... 详细信息

奶牛单位体重散热面积小,且汗腺不发达,在产奶过程中易产生大量热量,这会导致它们易遭受热应激,进而给奶牛带来多方面的不良影响,是制约奶业健康发展的重要因素之一。正常的免疫功能、消化道稳态和乳腺健康是奶牛发挥生产潜能的基本保障。热应激会严重抑制奶牛免疫功能,破坏消化道稳态,损害乳腺健康。本文系统综述了热应激对奶牛免疫功能、消化系统微生态和乳腺功能与健康的影响以及机制研究进展,旨在为缓解热应激对奶牛健康的损害,制定健康饲养管理方案提供理论参考。

关键词：热应激奶牛免疫功能消化功能乳腺健康

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Fabrication of Si-PDMS Low Voltage Capillary Electrophoresis Chip

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Engineering（科研） 2009年第2期1卷 111-116页

作者： Wenwen gu Zhiyu WEN Zhongquan WEN Yi XU fengfei LIANG Xiaoguo HU 不详

This paper discusses the fabrication of Si-PDMS low voltage capillary electrophoresis chip (CE chip). Arrayed-electrode which is used to apply low separation voltage is fabricated along the sidewalls of the separation channel on the silicon based bottom part. Isolation trenches, which are placed surrounding the arrayed-electrode, insure the insulation between the arrayed-electrode, as well as arrayed-electrode and liquid in the micro channel. Polydimethylsilicone (PDMS) is used as the cover. PDMS and silicon based bottom part are reversible sealed to attain Si-PDMS low voltage CE chip. Experiments have been done to obtain optimum electrophoresis separation condition: separation voltage is 45V, switch time is 2s and the Phe and Lys electrophoresis separation is successful.

关键词： Low Voltage Arrayed-Electrode Si-PDMS CE Chip

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抗生素对榆叶梅组培苗抑菌效果的影响

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吉林农业大学学报 2016年第1期38卷 53-56页

作者：李凤飞顾德峰吉林农业大学园艺学院长春130118

通过在培养基中添加单种抗生素或多种抗生素混合物,研究了抗生素对细菌污染的榆叶梅组培苗的影响。结果表明:当青霉素钠浓度300 mg/L、链霉素浓度100 mg/L 2种抗生素混合使用时,组培苗污染率相对较低、增殖率相对较高,且植株长势良好。

关键词：榆叶梅组织培养抗生素污染率

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施工过程中水回收与再利用技术的创新与应用

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建筑施工 2018年第12期40卷 2186-2188页

作者：张瑞顾伟东王凤飞中建二局第一建筑工程有限公司上海分公司上海200120

结合工程实际,收集基坑中的降排水、雨水、养护水等,再通过水泵将水引至施工区、加工场、生活区,以满足"生产、消防、降尘、车辆冲洗、场地道路冲洗、厕所排污冲洗"等应用,有效实现了节水。目前,通过工程实践与总结创新,已形成一套技术... 详细信息

结合工程实际,收集基坑中的降排水、雨水、养护水等,再通过水泵将水引至施工区、加工场、生活区,以满足"生产、消防、降尘、车辆冲洗、场地道路冲洗、厕所排污冲洗"等应用,有效实现了节水。目前,通过工程实践与总结创新,已形成一套技术先进、实用性强的施工工艺,可为类似工程提供借鉴。

关键词：施工水回收卫生间自动冲洗塔吊喷淋道路喷淋

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不同补铁方法联合重组人促红细胞生成素治疗肿瘤相关性贫血

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肿瘤基础与临床 2014年第2期27卷 137-139页

作者：古爱虎寇克韧朱凤飞芜湖市第一人民医院肿瘤内科安徽芜湖241001

目的探讨不同补铁方法联合重组人促红细胞生成素(rhEPO)治疗肿瘤相关性贫血的有效性。方法78例肿瘤相关性贫血患者随机分为3组:A组(n=26)予以rhEPO和琥珀酸亚铁,B组(n=26)予以rhEPO和每周1次的蔗糖铁,C组(n=26)予以rhEPO和每周2次的蔗... 详细信息

目的探讨不同补铁方法联合重组人促红细胞生成素(rhEPO)治疗肿瘤相关性贫血的有效性。方法78例肿瘤相关性贫血患者随机分为3组:A组(n=26)予以rhEPO和琥珀酸亚铁,B组(n=26)予以rhEPO和每周1次的蔗糖铁,C组(n=26)予以rhEPO和每周2次的蔗糖铁。比较治疗后3组患者的临床有效率、血红蛋白水平及KPS评分。结果治疗8周后B组总有效率达57.7%,C组总有效率达88.5%,高于A组的26.9%(P均<0.05),其中C组又高于B组(P<0.05);治疗8周后B组和C组血红蛋白水平、KPS评分改善情况均好于A组(P均<0.05),C组又明显好于B组(P<0.05)。结论补充铁剂联合rhEPO治疗肿瘤相关性贫血有较好的临床效果,其中静脉补充铁剂疗效优于口服,每周2次的静脉补充铁剂优于每周1次。

关键词：肿瘤贫血重组人促红细胞生成素铁剂

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可配置的业务流程动态管理系统应用研究

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计算机时代 2019年第8期 7-9页

作者：杨朔许锦才金凤飞古卫明浙江天正信息科技有限公司

Activiti是一个轻量级的工作流和业务流程管理(BPM)平台,作为面向企业业务的开源框架,可运行在任何Java应用程序、云服务器上。ExtJS是一种主要用于创建前端用户界面的ajax框架。PageOffice是协助用户在线编辑Word、Excel、PowerPoint... 详细信息

Activiti是一个轻量级的工作流和业务流程管理(BPM)平台,作为面向企业业务的开源框架,可运行在任何Java应用程序、云服务器上。ExtJS是一种主要用于创建前端用户界面的ajax框架。PageOffice是协助用户在线编辑Word、Excel、PowerPoint文档和Word/Excel模板的一种解决方案。文章研究了基于Activiti的可配置业务流程动态管理系统,该系统作为工作流开发基础平台,不仅适用于各种行业领域的业务流程管理软件,还可以通过动态配置工作流程适应不同的业务环境。

关键词： Activiti ExtJS PageOffice 业务流程动态配置

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