Blood neutrophil extracellular traps:a novel target for the assessment of mammary health in transition dairy cows

作     者：Luyi Jiang Huizeng Sun Fengfei Gu Jin He Fengqi Zhao Jianxin Liu Luyi Jiang;Huizeng Sun;Fengfei Gu;Jin He;Fengqi Zhao;Jianxin Liu

作者机构：Institute of Dairy ScienceCollege of Animal SciencesZhejiang UniversityHangzhouChina Ministry of Education Key Laboratory of Molecular Animal NutritionZhejiang UniversityHangzhouChina Institute of Animal Genetics and ReproductionCollege of Animal SciencesZhejiang UniversityHangzhouChina Department of Animal&Veterinary SciencesUniversity of VermontBurlingtonUSA 

出 版 物：《Journal of Animal Science and Biotechnology》 (畜牧与生物技术杂志（英文版）)

年 卷 期：2023年第14卷第3期

页      面：1068-1079页

核心收录：

学科分类：0905[农学-畜牧学] 09[农学] 

基　　金：financially supported by grants from the China-USA Intergovernmental Collaborative Project in S&T Innovation under the National Key R&D Program (No.2018YFE0111700 Beijing) 

主　　题：Blood-milk barrier Differential somatic cell count Mastitis risk Neutrophil extracellular traps 

摘      要：Background:Mammary health is important for transition dairy cows and has been well recognized to exert decisive effects on animal ***,the factors influencing mammary health are still *** somatic cell count(DSCC)could reflect the mastitis risk since it is the percentage of neutrophils plus lymphocytes in total somatic cells and could be reflective of mammary health of dairy *** work aimed to investigate the assessment and prognosis of the health of transition cows based on blood neutrophil extracellular traps(NETs).Results:Eighty-four transition Holstein dairy cows were *** serum was sampled in all the animals at week 1 pre-and postpartum,and milk was sampled at week 1 *** on the DSCC in milk at week 1,cows with lower(7.4%±4.07%,n=15)and higher(83.3%±1.21%,n=15)DSCCs were *** DSCC cows had higher levels of red blood cell counts(P0.05),hemoglobin(P=0.07),and hematocrit(P=0.05),higher concentrations of serum oxidative variables[reactive oxygen species(P0.05),malondialdehyde(P0.05),protein carbonyl(P0.05),and 8-hydroxy-2-deoxyguanosine(P=0.07)],higher levels of serum and milk NETs(P0.05)and blood-milk barrier indicators,including serumβ-casein(P=0.05)and milk immunoglobulin G2(P=0.09),than those of low DSCC *** addition,lower concentrations of serum nutrient metabolites(cholesterol and albumin)(P0.05)and a lower level of serum deoxyribonuclease I(P=0.09)were observed in high DSCC cows than in low DSCC *** the assessments performed using levels of the three prepartum serum parameters(NETs,deoxyribonuclease I andβ-casein),the area under the curve(0.973)of NETs was the *** addition,the sensitivity(1.00)and specificity(0.93)were observed for the discrimination of these cows using NETs levels with a critical value of 32.2 ng/mL(P0.05).Conclusions:The formation of NETs in blood in transition dairy cows may damage the integrity of the blood-milk barrier and thereby increase the risk for