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Glycogen synthase kinase 3 signaling in neural regeneration in vivo

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Journal of Molecular Cell Biology 2023年第12期15卷 1-11页

作者： Jing Zhang Shu-Guang Yang feng-quan zhou Sir Run Run Shaw Hospital Zhejiang University School of MedicineHangzhou 310016China

Glycogen synthase kinase 3(GSK3)signaling plays important and broad roles in regulating neural development in vitro and in ***,we reviewed recent findings of GSK3-regulated axon regeneration in vivo in both the peripheral and central nervous systems and discussed a few controversial findings in the ***,current evidence indicates that GSK3βsignaling serves as an important downstream mediator of the PI3K–AKT pathway to regulate axon regeneration in parallel with the mTORC1 ***,the mTORC1 pathway supports axon regeneration mainly through its role in regulating cap-dependent protein translation,whereas GSK3βsignaling might be involved in regulating N6-methyladenosine mRNA methylation-mediated,cap-independent protein *** addition,GSK3 signaling also plays a key role in reshaping the neuronal transcriptomic landscape during ***,we proposed some research directions to further elucidate the molecular mechanisms underlying the regulatory function of GSK3 signaling and discover novel GSK3 signaling-related therapeutic ***,we hope to provide an updated and insightful overview of how GSK3 signaling regulates neural regeneration in vivo.

关键词： GSK3 neural regeneration protein translation gene transcription

N6-methyladenine DNA Demethylase ALKBH1 Regulates Mammalian Axon Regeneration

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Neuroscience Bulletin 2021年第6期37卷 809-814页

作者： Qiao Li Cheng Qian Harry feng Tyger Lin Qingsan Zhu Ying Huang feng-quan zhou Department of Orthopedic Surgery Johns Hopkins University School of MedicineBaltimore 21205USA The Solomon H.Snyder Department of Neuroscience Johns Hopkins University School of MedicineBaltimore 21205USA Department of Spine Surgery The First Hospital of Jilin UniversityChangchun 130021China Department of Orthopedic Surgery China-Japan Union Hospital of Jilin UniversityChangchun 130031China

Dear Editor,DNA methylation is a key epigenetic regulatory approach for many biological processes,such as genomic imprinting,epigenetic memory maintenance,aging,and neural *** addition to 5-methylcytosine,DNA methylat... 详细信息

关键词： Regeneration development. adenine

Time course analysis of sensory axon regeneration in vivo by directly tracing regenerating axons

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Neural Regeneration Research 2020年第6期15卷 1160-1165页

作者： Yan Gao Yi-Wen Hu Run-Shan Duan Shu-Guang Yang feng-quan zhou Rui-Ying Wang Guilin Medical University GuilinGuangxi Zhuang Autonomous RegionChina Department of Orthopedic Surgery Affiliated Hospital of Guilin Medical UniversityGuilinGuangxi Zhuang Autonomous RegionChina Department of Orthopedics Henan Provincial People’s HospitalPeople’s Hospital of Zhengzhou UniversityZhengzhouHenan ProvinceChina Department of Orthopedic Surgery Johns Hopkins University School of MedicineBaltimoreMDUSA

Most current studies quantify axon regeneration by immunostaining regeneration-associated proteins,representing indirect measurement of axon lengths from both sensory neurons in the dorsal root ganglia and motor neurons in the spinal *** recently developed method of in vivo electroporation of plasmid DNA encoding for enhanced green fluorescent protein into adult sensory neurons in the dorsal root ganglia provides a way to directly and specifically measure regenerating sensory axon lengths in whole-mount nerves.A mouse model of sciatic nerve compression was established by squeezing the sciatic nerve with *** DNA carrying enhanced green fluorescent protein was transfected by ipsilateral dorsal root ganglion electroporation 2 or 3 days before *** distribution of dorsal root or sciatic nerve was observed by confocal *** 12 and 18 hours,and 1,2,3,4,5,and 6 days of injury,lengths of regenerated axons after sciatic nerve compression were measured using green fluorescence ***-related protein caspase-3 expression in dorsal root ganglia was determined by western blot *** found that in vivo electroporation did not affect caspase-3 expression in dorsal root *** root ganglia and sciatic nerves were successfully removed and subjected to a rapid tissue clearing *** soma in dorsal root ganglia expressing enhanced green fluorescent protein or fluorescent dye-labeled microRNAs were imaged after tissue *** results facilitate direct time course analysis of peripheral nerve axon *** study was approved by the Institutional Animal Care and Use Committee of Guilin Medical University,China(approval ***201503010)on March 7,2014.

关键词： axon regeneration cell apoptosis dorsal root ganglion in vivo electroporation micro RNAs peripheral nervous system sciatic nerve tissue clearing

Local manipulation of the growth cone cytoskeleton:new strategies to promote axon regeneration

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Neural Regeneration Research 2015年第12期10卷 1959-1960页

作者： Zi-wei Huang feng-quan zhou Saijilafu Department of Orthopedics the First Affiliated HospitalOrthopedic InstituteSoochow University Department of Orthopedic Surgery and the Solomon H.Snyder Department of Neuroscience the Johns Hopkins University School of Medicine

Thousands of spinal cord injuries occur worldwide as a result of traumatic or non-traumatic *** cord injury usually results in axonal damage,leading to permanent functional deficits and ***,axonal regeneration is the ... 详细信息

关键词： CSPG OMgp CNS

Inflammatory Factor IL1αInduces Aberrant Astrocyte Proliferation in Spinal Cord Injury Through the Grin2c/Ca^(2+)/CaMK2b Pathway

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Neuroscience Bulletin 2024年第4期40卷 421-438页

作者： Yu Xia Lu Ding Changlin Zhang Qi Xu Ming Shi Tianshun Gao feng-quan zhou David Y.B.Deng Scientific Research Center The Seventh Affiliated Hospital of Sun Yat-sen UniversityShenzhen518107China Department of Gynecology The Seventh Affiliated Hospital of Sun Yat-sen UniversityShenzhen518107China Pelvic Floor Disorders Center The Seventh Affiliated Hospital of Sun Yat-sen UniversityShenzhen518107China Big Data Center The Seventh Affiliated Hospital of Sun Yat-sen UniversityShenzhen518107China Sir Run Run Shaw Hospital Zhejiang University School of MedicineHangzhou310016China Orthopaedic and Neurological Repair Center The Seventh Affiliated Hospital of Sun Yat-sen UniversityShenzhen518107China

Spinal cord injury(SCI)is one of the most devastating traumas,and the aberrant proliferation of astrocytes usually causes neurological ***,the mechanism underlying astrocyte over-proliferation after SCI is ***2c(glutamate ionotropic receptor type 2c)plays an essential role in cell *** bioinformatic analysis indicated that Grin2c and Ca^(2+)transport functions were inhibited in astrocytes after *** of Grin2c stimulated astrocyte proliferation by inhibiting the Ca^(2+)/calmodulin-dependent protein kinase 2b(CaMK2b)pathway in *** screening different inflammatory factors,interleukin 1α(IL1α)was further found to inhibit Grin2c/Ca^(2+)/CaMK2b and enhance astrocyte proliferation in an oxidative damage *** of IL1αusing neutralizing antibody resulted in increased Grin2c expression and the inhibition of astrocyte proliferation ***,this study suggests that IL1αpromotes astrocyte proliferation by suppressing the Grin2c/Ca^(2+)/CaMK2b pathway after SCI,revealing a novel pathological mechanism of astrocyte proliferation,and may provide potential targets for SCI repair.

关键词： IL1α Grin2c Astrocyte Spinal cord injury

Neuronal Histone Methyltransferase EZH2 Regulates Neuronal Morphogenesis,Synaptic Plasticity,and Cognitive Behavior in Mice

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Neuroscience Bulletin 2023年第10期39卷 1512-1532页

作者： Mei Zhang Yong Zhang Qian Xu Joshua Crawford Cheng Qian Guo-Hua Wang Jiang Qian Xin-Zhong Dong Mikhail V.Pletnikov Chang-Mei Liu feng-quan zhou Department of Orthopaedic Surgery Johns Hopkins University School of MedicineBaltimore 21205USA The Solomon H.Snyder Department of Neuroscience Johns Hopkins University School of MedicineBaltimore 21205USA Department of Psychiatry and Behavioral Sciences Johns Hopkins University School of MedicineBaltimore 21205USA Department of Ophthalmology Johns Hopkins University School of MedicineBaltimore 21205USA School of Life Sciences Division of Life Sciences and MedicineUniversity of Science and Technology of ChinaHefei 230026China State Key Laboratory of Reproductive Biology Institute of ZoologyChinese Academy of SciencesBeijing 100190China Sir Run Run Shaw Hospital Zhejiang University School of MedicineHangzhou 310016China

The histone methyltransferase enhancer of zeste 2 polycomb repressive complex 2 subunit(EZH2)-mediated trimethylation of histone H3 lysine 27(H3K27me3)regulates neural stem cell proliferation and fate specificity through silencing different gene sets in the central nervous ***,we explored the function of EZH2 in early post-mitotic neurons by generating a neuron-specific Ezh2 conditional knockout mouse *** results showed that a lack of neuronal EZH2 led to delayed neuronal migration,more complex dendritic arborization,and increased dendritic spine *** analysis revealed that neuronal EZH2-regulated genes are related to neuronal *** particular,the gene encoding p21-activated kinase 3(Pak3)was identified as a target gene suppressed by EZH2 and H3K27me3,and expression of the dominant negative Pak3 reversed Ezh2 knockout-induced higher dendritic spine ***,the lack of neuronal EZH2 resulted in impaired memory behaviors in adult *** results demonstrated that neuronal EZH2 acts to control multiple steps of neuronal morphogenesis during development,and has long-lasting effects on cognitive function in adult mice.

关键词： Neural development Dendritic branching Dendritic spine Cognitive function Epigenetics Histone methylation EZH2

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汛期地质灾害气象预警自动化的一种实现途径

安徽农业科学 2008年第20期36卷 8746-8748页

作者：冯全洲中国地质大学环境学院

在分析常用的预报预警建模方法存在问题的基础上,以河南省为例,提出了汛期地质灾害气象预警自动化的一种实现途径。

关键词：汛期地质灾害预警自动化预警过程识别预警模型

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刚构-单肋拱组合桥梁的稳定性研究

振动与冲击 2008年第12期27卷 122-125页

作者：谭红霞陈政清封周权湖南大学风工程试验研究中心长沙410082 湘潭大学土木工程与力学学院湖南湘潭411105

刚构-单肋拱组合桥梁是一种新型的桥梁结构型式,稳定性问题是不可避免的。通过一座实桥建立有限元模型,分析了恒载、风载和列车荷载等荷载组合作用下的第一类稳定问题和考虑几何、材料等非线性因素恒载作用下的第二类稳定问题,并探讨了... 详细信息

刚构-单肋拱组合桥梁是一种新型的桥梁结构型式,稳定性问题是不可避免的。通过一座实桥建立有限元模型,分析了恒载、风载和列车荷载等荷载组合作用下的第一类稳定问题和考虑几何、材料等非线性因素恒载作用下的第二类稳定问题,并探讨了影响整体稳定的有关因素,得出材料非线性和非保向力效应对刚构-单肋拱组合桥梁的稳定性影响较大,在同类桥型的设计中应予以重视。

关键词：刚构钢箱拱肋单肋拱稳定性

基于自由振动响应识别桥梁断面颤振导数的人工蜂群算法

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工程力学 2020年第2期37卷 192-200页

作者：林阳封周权华旭刚陈政清湖南大学风工程与桥梁工程湖南省重点实验室

基于桥梁节段模型风洞试验自由振动衰减时程信号,提出了桥梁断面颤振导数识别的人工蜂群算法。基于最小二乘原理,将竖弯和扭转信号的整体残差平方和作为目标函数,使用人工蜂群算法对相关参数进行寻优搜索,识别出桥梁断面的颤振导数。与... 详细信息

基于桥梁节段模型风洞试验自由振动衰减时程信号,提出了桥梁断面颤振导数识别的人工蜂群算法。基于最小二乘原理,将竖弯和扭转信号的整体残差平方和作为目标函数,使用人工蜂群算法对相关参数进行寻优搜索,识别出桥梁断面的颤振导数。与其他迭代算法相比,人工蜂群算法是受生物启发产生的寻优算法,对初值没有要求,从而避免了迭代初值对识别精度的影响。为考察人工蜂群算法在桥梁断面颤振导数识别中的有效性,进行了理想平板模型仿真以及某大桥节段模型风洞试验,结果表明,桥梁断面颤振导数识别的人工蜂群算法具有较好的稳定性和可靠性。

关键词：桥梁工程颤振导数人工蜂群算法参数识别优化方法