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In vitro maturation of human oocytes maintaining good development potential for rescue intracytoplasmic sperm injection with fresh sperm

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World Journal of Clinical Cases 2022年第7期10卷 2166-2173页

作者： Yun-Qiao Dong Chuang-Qi Chen Yu-Qiang huang Dun liu Xi-Qian Zhang feng-hua liu Department of Assisted Reproductive Center Guangdong Women and Children HospitalGuangzhou 511400Guangdong ProvinceChina

BACKGROUND The outcomes of the use of commercial in vitro maturation(IVM)medium to culture immature oocytes obtained from conventional ovulation induction,followed by rescue intracytoplasmic sperm injection(RICSI),are not ideal.It is thus difficult to widely adopt this approach in clinical practice.Therefore,it is necessary to explore methods for improving the clinical outcome of IVM.AIM To study the effect of sperm on the developmental potential of in vitro-matured oocytes in conventional culture.METHODS This was a retrospective study of patients whose immature oocytes were harvested from conventional oocyte stimulation cycles and underwent ICSI at our hospital between June 2018 and August 2020.RICSI was performed using sperm collected on the day of oocyte harvest(old)and sperm collected on the day of RICSI(fresh)and oocytes matured in vitro after 24 h of culture in conventional medium.The rates of in vitro oocyte maturation,normal fertilization,normal cleavage,day-3 top-quality embryos,and useful blastocyst formation were compared between the two groups.RESULTS In total,102 germinal vesicle(GV)-stage immature oocytes were cultured in the old sperm group.In the fresh sperm group,122 GV-stage immature oocytes were collected and cultured in vitro for 24 h.There were no significant differences in the general conditions of males and females between the two groups(P>0.05).The oocyte maturation,normal fertilization,and normal cleavage rates of the old and fresh groups were 51.0%vs 55.7%,61.5%vs 64.7%,and 93.8%vs 93.2%,respectively.None of the rates differed significantly(P>0.05)between the two groups.However,the day-3 top-quality embryo and useful blastocyst rates of the old and fresh sperm groups were 16.6%vs 63.4%;6.67%vs 34.6%,respectively.The day-3 top-quality embryos and useful blastocyst rates of the old sperm group were significantly lower than those of the fresh group(P<0.05).CONCLUSION In vitro maturation with conventional culture medium combined with the use of fresh sperm collected on the day of RICSI is an

关键词： In vitro oocyte maturation Sperm injections Intracytoplasmic Semen analysis In vitro fertilization Human oocyte

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Epidemiological Survey and Risk Factor Analysis of Recurrent Spontaneous Miscarriages in Infertile Women at Large Infertility Centers

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Chinese Medical Journal 2017年第17期130卷 2056-2062页

作者： Hai-Yan Wang Jie Qiao Xiao-Xi Sun Shu-Yu Wang Xiao-Yan Liang Yun Sun feng-hua liu Reproductive Medical Center Department of Obstetrics and Gynecology Peking University Third Hospital Beijing 100191 China Shanghai Ji Ai Diagnosis and Treatment Center for Genetics and Infertility Shanghai 200011 China Reproductive Medical Center Beijing Obstetrics and Gynecology Hospital Beijing 100006 China Reproductive Medical Center The Sixth Affiliated Hospital Sun Yat-sen University Guangzhou Guangdong 510665 China Reproductive Medical Center Shanghai Jiao Tong University School of Medicine Affiliated Renji Hospital Shanghai 200011 China Reproductive Medical Center Maternal and Child Health Hospital of Guangdong Province Guangzhou Guangdong 511400 China

Background：A higher frequency of spontaneous miscarriage has been observed in infertile couples,and there is a higher prevalence of infertility among patients with a history of recurrent spontaneous miscarriages （RSMs;〉2 miscarriages）.This study aimed to determine the proportion of infertile patients with RSM and examine risk factors associated in patients with RSM being treated with assisted reproductive technologies.Methods：This cross-sectional observational study was conducted at six reproductive medicine centers in three cities of China.Data of 751 patients with at least one spontaneous miscarriage were analyzed.Demographic data and etiological factors associated with infertility were compiled and compared between patients with a single spontaneous miscarriage （SSM） and those with RSM.Results：Two hundred （26.6%,95% confidence interval [CI]：23.50-29.95%） patients experienced RSMs and 551 （73.4%） had a single miscarriage.The odds of RSM increased with increasing age （odds ratio [OR] =1.06）,uterine disorders （OR =2.09）,endocrine disorders （OR =2.48）,and immune disorders （OR =2.98）.Higher education level,masters or above,and a pelvic cavity disorder were associated with lower risk of RSM （OR =0.27 and 0.46,respectively）.Late spontaneous miscarriages were more frequent in patients with RSM than in those with a SSM （31.5% vs.14.2%,respectively,P 〈 0.001） and were associated with a history of uterine cavity procedures （OR =2.095） and cervical factors related to infertility （OR =4.136,95% CI：1.012-16.90）.Conclusions：Compared to patients with only a SSM,the conditions of patients with RSM are more complicated.To increase the success rate of assisted reproductive technology,factors including uterus cavity adhesion,cervical relaxation,endocrine disorders,and immune disorders should be treated before assisted reproduction is initiated.These data may provide treatment guidance for infertile patients with a history of RSM.

关键词： Assisted Reproductive Technology Epidemiological Survey Infertility Recurrent Spontaneous Miscarriage Risk Factor

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SYNTHESES　OF　Ph　AND　TEMPERATURE－SENSITIVE　HYDROGELS　AND　THEIR　APPLICATIONS　IN　CONTROLLED　RELEASE　OF　BIOMACROMOLECULES

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Chinese Chemical Letters 1995年第1期6卷 35-38页

作者： feng liu feng hua liu Guo Liang TAO Rem Xi ZHUO Department of Chemistry, Wuhan University. Wuhan. 430072 Wuhan Univ Dept Chem Wuhan 430072 Peoples R China

A series of pH- and temperature-sensitive hydrogels were prepared These gels do not swell at 37℃ and pH 1.4 but swell at 37℃ and pH 7 4.When papain was loaded in these gels. it can be released at 37℃ and pH 7 4in a... 详细信息

关键词： Ph OF SYNTHESES TEMPERATURE APPLICATIONS AND

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Overview and a meta-analysis of pancreatic melanoma based on case reports

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World Journal of Meta-Analysis 2014年第2期2卷 36-41页

作者： Zhan-Na Yuan feng-hua liu Yong Tang Department of Pancreatic Cancer Key Laboratory of Cancer Prevention and Therapy Tianjin Medical University Cancer Institute and Hospital Department of Gynecology Key Laboratory of Cancer Prevention and Therapy Tianjin Medical University Cancer Institute and Hospital

AIM:To investigate the clinical characteristics of pancreatic malignant melanoma.METHODS:Here we report a case of pancreatic malignant melanoma and present a meta-analysis of pancreatic melanoma based on data from well-documented case reports.RESULTS:A 32-year-old male presented with abdominal pain and jaundice.Computed tomography showed a non-discrete mass in the head of the pancreas.A pancreatoduodenectomy was performed.Histology revealed pancreatic malignant melanoma.Immunostaining showed that the neoplastic cells were positive for Melan A and S100,and that they expressed HMB-45.A review of 13 publications reporting 15 cases identified main clinical symptoms as abdominal pain(nine cases)and jaundice(three cases).Nine patients had a historyof extra-abdominal malignant melanoma.The overall median survival time was 10 mo.CONCLUSION:Pancreatic malignant melanoma is not typified by any special clinical presentation.The diagnosis depends on biopsy or specimen histology.Surgical resection remains the best treatment choice.

关键词： Pancreatic malignant melanoma Survival Diagnosis Treatment Review

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A NOVEL POLYMER ENZYME LINKED IMMUNOASSAY METHOD AND ITS APPLICATIONS

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Chinese Chemical Letters 1994年第4期5卷 325-326页

作者： feng liu feng hua liu Ren Xi ZHUO Yan Zhuo DENG Yun E ZHEN (Department of Chemistry, Wuhan Univ., Wuhan 430072)

During the course of study, we found that both poly (N-isopropylacrylamide ) (PNIP) and PNIP-Ab (enzyme-labelled antibody)could be adhere tightly to a cellulose acetale-nitrate membrane, and that the retention of PNIP-Ab on the membrane increased over 30-fold when compared with the unconjugated Ab.Thus we used this characteristic to develop a novel immunoassay method-polymer enzyme linked immunoassay method: homogeneous antigen-antibody reaction and heterogeneous separation process. When applied for detection of human serum HBsAg, this immunoassay system can detect as little as 1 ng/ml of human serum HBsAg.

关键词： ITS A NOVEL POLYMER ENZYME LINKED IMMUNOASSAY METHOD AND ITS APPLICATIONS

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安石榴苷对脂多糖诱导奶牛子宫内膜上皮细胞炎症损伤的保护作用（英文）

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2017年第6期18卷 481-491页

作者： An LYU Jia-jia CHEN Hui-chuan WANG Xiao-hong YU Zhi-cong ZHANG Ping GONG Lin-shu JIANG feng-hua liu Beijing Key Laboratory for Dairy Cow Nutrition College of Animal Science and TechnologyBeijing University of AgricultureBeijing 102206China

目的:评估安石榴苷对脂多糖诱导奶牛子宫内膜上皮细胞炎症损伤的保护作用,并初步探讨其作用机制。创新点:首次证明安石榴苷对脂多糖诱导奶牛子宫内膜上皮细胞炎症损伤具有保护作用,且此作用与核转录因子κB(NF-κB)和丝裂原活化蛋白激酶... 详细信息

目的:评估安石榴苷对脂多糖诱导奶牛子宫内膜上皮细胞炎症损伤的保护作用,并初步探讨其作用机制。创新点:首次证明安石榴苷对脂多糖诱导奶牛子宫内膜上皮细胞炎症损伤具有保护作用,且此作用与核转录因子κB(NF-κB)和丝裂原活化蛋白激酶(MAPK)信号通路的抑制相关。方法:用不同浓度的脂多糖(1、10、30、50和100μg/ml)刺激奶牛子宫内膜上皮细胞3、6、9、12和18 h,筛选出建立炎症损伤的最佳作用浓度和时间。安石榴苷预处理细胞2 h后用脂多糖刺激12 h,用逆转录聚合酶链式反应(RT-PCR)检测炎症因子白细胞介素1β(IL-1β)、白细胞介素6(IL-6)、白细胞介素8(IL-8)及肿瘤坏死因子α(TNF-α)的表达。用蛋白质免疫印迹试验(Western blotting)的方法检测核因子κB抑制蛋白α(IκBα)、磷酸化的p65、p38、c-Jun氨基末端激酶(JNK)和细胞外调节蛋白激酶(ERK)的表达水平。结论:MTT结果显示,30μg/ml脂多糖刺激奶牛子宫内膜上皮细胞12 h能够造成细胞活力下降和形态改变(图2和3)。RT-PCR结果显示,安石榴苷预处理后炎症因子显著降低(图4)。Western blotting结果显示,安石榴苷预处理能显著抑制IκBα降解以及p65、p38、JNK和ERK的磷酸化表达水平(图5和6)。综上所述,安石榴苷对脂多糖诱导奶牛子宫内膜上皮细胞炎症损伤具有保护作用,在治疗奶牛子宫内膜炎中具有重要价值。

关键词：奶牛子宫内膜上皮细胞炎症因子炎性损伤安石榴苷

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厚朴酚缓解热应激诱导的小肠上皮细胞损伤及其分子机制（英文）

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2016年第6期17卷 413-424页

作者： Chen MEI Sha-sha HE Peng YIN Lei XU Ya-ran SHI Xiao-hong YU An LYU feng-hua liu Lin-shu JIANG Beijing Key Laboratory for Dairy Cow Nutrition College of Animal Science and Technology Beijing University of Agriculture College of Veterinary Medicine China Agricultural University (CAU)

目的:探讨厚朴酚(Mag)对热应激大鼠小肠上皮细胞(IEC-6)修复作用,并初步探讨其作用机制。创新点:首次在大鼠小肠上皮细胞热应激模型中证明厚朴酚可明显降低细胞损伤程度,且此作用与细胞周期G1期相关。方法:本试验采用大鼠小肠上皮细胞(I... 详细信息

目的:探讨厚朴酚(Mag)对热应激大鼠小肠上皮细胞(IEC-6)修复作用,并初步探讨其作用机制。创新点:首次在大鼠小肠上皮细胞热应激模型中证明厚朴酚可明显降低细胞损伤程度,且此作用与细胞周期G1期相关。方法:本试验采用大鼠小肠上皮细胞(IEC-6)为研究对象,将其分为五组:对照组(37°C,5%CO2),热应激组(42°C,3 h),热应激+厚朴酚低浓度组(5μmol/L),热应激+厚朴酚中浓度组(10μmol/L),热应激+厚朴酚高浓度组(20μmol/L)。采用MTS法复制热应激模型及厚朴酚药物浓度筛选;采用流式细胞术检测热应激造成大鼠小肠上皮细胞细胞周期阻滞及不同浓度厚朴酚缓解细胞周期阻滞情况;利用电子显微镜观察热应激造成的细胞损伤情况;利用荧光电子显微镜观察Ed U染色后,热应激对细胞增殖情况的影响及不同浓度厚朴酚的修复作用;采用荧光定量聚合酶链反应(PCR)技术检测热应激对细胞周期基因表达影响及不同浓度厚朴酚对细胞周期基因调节作用,采用蛋白质免疫印迹(Western blot)技术检测热应激对细胞周期蛋白表达的影响及不同浓度厚朴酚对细胞周期蛋白修复作用。结论:流式细胞术显示热应激造成IEC-6细胞周期阻滞在G1期(96.5%),而三种浓度厚朴酚均可以缓解细胞周期阻滞现象且呈剂量依赖性(5μmol/L88.8%,10μmol/L 81.0%,20μmol/L 73.5%)。热应激导致阻滞细胞分裂的G1期基因p21、p27和Rb显著上调(P<0.01),显示细胞周期阻滞,不同浓度厚朴酚下调这三个基因表达且呈剂量依赖性;促进细胞分裂的G1期基因E2F1、CDK4和cyclin D1表达显著下调(P<0.01),显示细胞周期阻滞不能正常增值,厚朴酚有效上调这三个基因表达水平。热应激导致G1期阻滞细胞分裂蛋白p21及p27上调显著(P<0.01),厚朴酚有效下调p21及p27蛋白表达,且在20μmol/L浓度时效果最佳;而G1期促进细胞分裂蛋白p Rb、E2F1、CDK4和cyclin D1(CCND1)在热应激下表达显著下调(P<0.01),厚朴酚具有一定上调以上四个蛋白的作用,且在20μmol/L浓度时效果最佳。厚朴酚作为天然成分药物,通过缓解热应激造成的IEC-6细胞G1期细胞周期阻滞,具有缓解热应激造成的细胞损伤能力,有望作为预防畜禽热应激的饲料添加剂。

关键词：细胞周期阻滞细胞损伤热应激 IEC-6 厚朴酚

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益母散通过增加催产素水平和子宫平滑肌缝隙连接蛋白43的表达提高孕鼠分娩能力（英文）

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2017年第11期18卷 986-993页

作者： Qi-huan WANG Shuang ZHANG Li-meng QIN Wen-jun ZHANG feng-hua liu Jian-qin XU Yun-fei MA Ke-dao TENG College of Veterinary Medicine China Agricultural University College of Animal Science and Technology Beijing University of Agriculture

目的:评价益母散对孕鼠分娩能力的促进作用,并初步探讨其作用机制。创新点:首次在临近分娩小鼠中证明新中兽医药处方益母散可明显促进孕鼠分娩能力,且此作用与催产素和子宫平滑肌缝隙连接蛋白43(Cx43)的高表达相关。方法:将妊娠小鼠分... 详细信息

目的:评价益母散对孕鼠分娩能力的促进作用,并初步探讨其作用机制。创新点:首次在临近分娩小鼠中证明新中兽医药处方益母散可明显促进孕鼠分娩能力,且此作用与催产素和子宫平滑肌缝隙连接蛋白43(Cx43)的高表达相关。方法:将妊娠小鼠分为对照组和益母散低、中、高剂量组,产前3天持续灌胃,统计孕鼠分娩产程和死胎率。采用离体平滑肌张力试验测定子宫平滑肌张力,用酶联免疫吸附测定(ELISA)方法检测血清中催产素和前列腺素E2(PGE2)含量,用蛋白免疫印迹(Western blotting)及免疫组织化学等方法检测小鼠子宫平滑肌中Cx43含量。结论:益母散灌服处理能够使小鼠分娩产程缩短和死胎率降低(图1),并增强分娩小鼠子宫平滑肌张力(图2)。ELISA实验结果显示,益母散灌服处理使分娩小鼠血清催产素水平显著提高(图3)。免疫组化和免疫印迹实验结果显示,益母散灌服处理使分娩小鼠子宫平滑肌Cx43含量显著提高(图4和5)。综上所述,益母散具有提高孕鼠分娩能力的作用,该作用与催产素水平和子宫平滑肌Cx43的高表达相关。

关键词：益母散催产素前列腺素E2 缝隙连接蛋白43

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A review of high-performance MEMS sensors for resource exploration and geophysical applications

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Petroleum Science 2022年第6期19卷 2631-2648页

作者： hua-feng liu Zhi-Cai Luo Zhong-Kun Hu Shan-Qing Yang Liang-Cheng Tu Ze-Bing Zhou Michael Kraft National Precise Gravity Measurement Facility(PGMF)and School of Physics Huazhong University of Science and TechnologyWuhanHubei430074China Institute of Geophysics Huazhong University of Science and TechnologyWuhanHubei430074China TianQin Research Center for Gravitational Physics and School of Physics and Astronomy Sun Yat-sen University(Zhuhai Campus)ZhuhaiGuangdong519082China Nanchang Research Institute Sun Yat-sen UniversityNanchangJiangxi330099China ESAT-MNS University of LeuvenLeuvenBelgium

MEMS sensors have the advantages of small volume,lightweight,and low cost,therefore,have been widely used in the fields of consumer electronics,industry,health,defence,and aerospace.With their ever-improving performance,MEMS sensors have also started to be used in resource exploration and geophysical applications.However,the requirements of high-precision MEMS sensors for geophysical applications have not been specified in detail.Therefore,this paper systematically analyzes the requirements of high-performance MEMS sensors for prospecting and geophysical applications,including seismic surveillance,Earth tide,volcanic activity monitoring for natural disasters;seismic,gravity,and magnetic resource prospecting;drilling process monitoring and local gravity measurement for gravity aided navigation.Focusing on the above applications,this paper summarizes the state-of-the-art of research on high-performance MEMS sensors for resource exploration and geophysical applications.Several off-the-shelf MEMS sensors have been used for earthquake monitoring,seismic exploration and drilling process monitoring,and a range of MEMS research prototype sensors have successfully been employed for Earth tides measurement and are promising to be used for gravity exploration.MEMS magnetometers should have a lower noise floor to meet the demand for magnetic exploration.MEMS gravity gradiometers are still under early development and will not be deployable in short-term.Highperformance MEMS sensors hold the advantages of low-cost,high integration,and capability of working in extreme environments;therefore,they are likely to gradually replace some conventional geophysical instruments in some application areas.

关键词： Accelerometer Seismometer Gravimeter Tilt-meter Magnetometer

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A　NOVEL　POLYMER　ENZYME　LINKED　IMMUNOBLOTTING　ASSAY　METHOD　AND　ITS　APPLICATIONS（Ⅱ）

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Chinese Chemical Letters 1994年第5期5卷 387-388页

作者： feng hua liu feng liu Yan Zhuo DENG Yun E ZENG Ren Xi ZHUO (Dept. of Chem., Dept. of Anal. and Testing Scl.,Wuhan Univ., Wuhan,430072)

By using the characteristic that poly (N-isopropylacrylamide) can be adhered to callulose acetais-nitrate membrane in high retentior tightly and ramdly,we developed a novel immunoamay method- polymer enayme Hnked immu... 详细信息

关键词： ITS METHOD APPLICATIONS AND

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