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Liberation of SARS-CoV main protease from the viral polyprotein:N-terminal autocleavage does not depend on the mature dimerization mode

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Protein & Cell 2010年第3期1卷 59-74页

作者： Shuai Chen felix jonas Can Shen Rolf Higenfeld Institute of Biochemistry Center for Structural and Cell Biology in MedicineUniversity of LübeckRatzeburger Allee 16023538 LübeckGermany Laboratory for Structural Biology of Infection and Inflammation c/o DESYBuilding 22aNotkestr.8522603 HamburgGermany

The main protease(M^(pro))plays a vital role in proteolytic processing of the polyproteins in the replicative cycle of SARS coronavirus(SARS-CoV).Dimerization of this enzyme has been shown to be indispensable for transcleavage ***,the auto-processing mechanism of M^(pro),*** own release from the polyproteins through autocleavage,remains *** study elucidates the relationship between the N-terminal autocleavage activity and the dimerization of“immature”M^(pro).Three residues(Arg4,Glu290,and Arg298),which contribute to the active dimer conformation of mature M^(pro),are selected for mutational ***,all three mutants still perform N-terminal autocleavage,while the dimerization of mature protease and transcleavage activity following auto-processing are completely inhibited by the E290R and R298E mutations and partially so by the R4E ***,the mature E290R mutant can resume N-terminal autocleavage activity when mixed with the“immature”C145A/E290R double mutant whereas its trans-cleavage activity remains ***,the N-terminal auto-processing of M^(pro) appears to require only two“immature”monomers approaching one another to form an“intermediate”dimer structure and does not strictly depend on the active dimer conformation existing in mature *** conclusion,an auto-release model of M^(pro) from the polyproteins is proposed,which will help understand the auto-processing mechanism and the difference between the autocleavage and trans-cleavage proteolytic activities of SARS-CoV M^(pro).

关键词： SARS-CoV M^(pro) N-terminal autocleavage autocleavage activity trans-cleavage activity dimerization

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Artificial intelligence in pancreatic surgery:current applications

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Journal of Pancreatology 2023年第2期6卷 74-81页

作者： Christoph Kuemmerli Fabian Rössler Caroline Berchtold Michael C.Frey Alexander Studier-Fischer Amila Cizmic Jan Philipp jonas Thilo Hackert felix Nickel Philip C.Müller Department of Surgery Clarunis-University Centre for Gastrointestinal and Liver DiseasesBaselSwitzerland Swiss HPB and Transplantation Center Department of Surgery and TransplantationUniversity Hospital ZurichZurichSwitzerland Department for General Visceral and Vascular SurgeryKantonsspital BadenBadenSwitzerland Department of General Visceral and Transplantation SurgeryHeidelberg University HospitalHeidelbergGermany Department of General Visceral and Thoracic SurgeryUniversity Medical Center Hamburg-EppendorfHamburgGermany

Surgery plays a central role in the treatment of benign and malignant pancreatic *** intelligence(AI)is an important upcoming technology to support surgeons in pre-,intra-,and postoperative diagnosis,decision-making and training toward an optimized patient *** AI applications show a promising role in the evaluation of preoperative images for prediction of malignancy and resectability,intraoperative decision support,surgical training as well as a postoperative risk stratification to per-sonalize the management of *** scoping review summarizes the most up to date developments of AI in pancreatic surgery with the highest available level of evidence.

关键词： Artificial intelligence Image-guidance Machine learning Pancreas neoplasms Pancreatic ductal carcinoma Personalized medicine Surgery

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Investigating the consequences of asymmetric endoplasmic reticulum inheritance in Saccharomyces cerevisiae under stress using a combination of single cell measurements and mathematical modelling

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Synthetic and Systems Biotechnology 2018年第1期3卷 64-75页

作者： felix R.H.jonas Kate E.Royle Rochelle Aw Guy-Bart V.Stan Karen M.Polizzi Department of Bioengineering Imperial College LondonLondonSW72AZUnited Kingdom Imperial College Centre for Synthetic Biology LondonSW72AZUnited Kingdom Department of Life Sciences Imperial College LondonLondonSW72AZUnited Kingdom

Adaptation allows organisms to maintain a constant internal environment,which is optimised for *** unfolded protein response(UPR)is an example of a feedback loop that maintains endoplasmic reticulum(ER)homeostasis,and is characteristic of how adaptation is often mediated by transcriptional *** more recent discovery of asymmetric division in maintaining ER homeostasis,however,is an example of how alternative non-transcriptional pathways can exist,but are overlooked by gold standard transcriptomic or proteomic population-based *** this study,we have used a combination of fluorescent reporters,flow cytometry and mathematical modelling to explore the relative roles of asymmetric cell division and the UPR in maintaining ER *** low ER stress,asymmetric division leaves daughter cells with an ER deficiency,necessitating activation of the UPR and prolonged cell cycle during which they can recover ER functionality before *** analysis of and simulation results from our mathematical model reinforce the experimental observations that low ER stress primarily impacts the growth rate of the daughter *** results demonstrate the interplay between homeostatic pathways and the importance of exploring sub-population dynamics to understand population adaptation to quantitatively different stresses.

关键词： Adaptation Asymmetric cell division Endoplasmic reticulum stress Unfolded protein response Saccharomyces cerevisiae

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Serotonin type 3 receptor subunit gene polymorphisms associated with psychosomatic symptoms in irritable bowel syndrome:A multicenter retrospective study

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World Journal of Gastroenterology 2022年第21期28卷 2334-2349页

作者： Sabrina Berens Yuanjun Dong Nikola Fritz Jutta Walstab Mauro D'Amato Tenghao Zheng Verena Wahl felix Boekstegers Justo Lorenzo Bermejo Cristina Martinez Stefanie Schmitteckert Egbert Clevers Felicitas Engel Annika Gauss Wolfgang Herzog Robin Spiller Miriam Goebel-Stengel Hubert Mönnikes Viola Andresen Frieling Thomas Jutta Keller Christian Pehl Christoph Stein-Thöringer Gerard Clarke Timothy G Dinan Eamonn M Quigley Gregory Sayuk Magnus Simrén jonas Tesarz Gudrun Rappold Lukas van Oudenhove Rainer Schaefert Beate Niesler Department of General Internal Medicine and Psychosomatics University Hospital HeidelbergHeidelberg 69120Germany Department of Human Molecular Genetics Institute of Human GeneticsUniversity of HeidelbergHeidelberg 69120Germany Department of General Internal Medicine and Psychosomatics Internal Medicine IIUniversity Hospital HeidelbergHeidelberg 69120Germany Department of Human Molecular Genetics University of HeidelbergHeidelberg 69120Germany Gastrointestinal Genetics Lab CIC bioGUNE-BRTADerio 48160Spain IKERBASQUE Basque Foundation for ScienceBilbao 48001Spain Unit of Clinical Epidemiology Department of Medicine SolnaKarolinska InstitutetStockholm 17177Sweden Institute of Medical Biometry and Informatics Heidelberg UniversityHeidelberg 69120Germany Lleida Institute for Biomedical Research Dr.PifarréFoundation(IRBLleida) Av.Alcalde Rovira RoureLleida 25198Spain Department of Clinical and Experimental Medicine Translational Research Center for Gastrointestinal DisordersKU LeuvenLeuven 3000Belgium Department of Gastroenterology Infectious Diseases and IntoxicationsUniversity of HeidelbergHeidelberg 69120Germany Department of General Internal Medicine and Psychosomatics Heidelberg UniversityHeidelberg 69120Germany Nottingham Digestive Diseases Centre University of NottinghamNottingham NG72QLUnited Kingdom Helios Klinikum Rottweil Rottweil 78628Germany Department of Medicine Institute of Neurogastroenterology(H.M.)Martin-Luther-HospitalBelin 14193Germany Israelitisches Krankenhaus in Hamburg Hamburg 22297Germany Internal Medicine II Helios Klinikum KrefeldKrefeld 47805Germany Israelitisches Krankenhaus Hamburg Hamburg 22297Ghana Krankenhaus Vilsbiburg Vilsbiburg 84137Germany Division of Microbiome and Cancer German Cancer Research Center(DKFZ)Heidelberg 69120Germany Department of Psychiatry and Neurobehavioral Science University College CorkCork T23Ireland Medicine in Digestive Disorders Department of MedicineLynda K.and David M.Underwood Center for Digestive DisordersHou

BACKGROUND Single-nucleotide polymorphisms(SNPs)of the serotonin type 3 receptor subunit(HTR3)genes have been associated with psychosomatic symptoms,but it is not clear whether these associations exist in irritable bowel syndrome(IBS).AIM To assess the association of HTR3 polymorphisms with depressive,anxiety,and somatization symptoms in individuals with *** In this retrospective study,623 participants with IBS were recruited from five specialty centers in Germany,Sweden,the United States,the United Kingdom,and ***,anxiety,and somatization symptoms and sociodemographic characteristics were *** functional SNPs—HTR3A c.-42C>T,HTR3B c.386A>C,HTR3C c.489C>A,and HTR3E c.*76G>A—were genotyped and analyzed using the dominant and recessive *** also performed separate analyses for sex and IBS *** scores were calculated as the number of minor alleles of the SNPs *** impact of HTR3C c.489C>A was tested by radioligand-binding and calcium influx *** Depressive and anxiety symptoms significantly worsened with increasing numbers of minor HTR3C c.489C>A alleles in the dominant model(F_(depressive)=7.475,P_(depressive)=0.006;F_(anxiety)=6.535,P_(anxiety)=0.011).A higher SNP score(range 0-6)was linked to a worsened depressive symptoms score(F=7.710,P-linear trend=0.006)in *** potential relevance of the HTR3C SNP was corroborated,showing changes in the expression level of 5-HT3AC variant *** We have provided the first evidence that HTR3C c.489C>A is involved in depressive and anxiety symptoms in individuals with *** SNP score indicated that an increasing number of minor alleles is linked to the worsening of depressive symptoms in IBS.

关键词： Irritable bowel syndrome 5-HT3 receptor subunit gene polymorphisms Single-nucleotide polymorphism score Depression Anxiety Somatization

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Enhancing Tomato (Solanum lycopersicum L.) Fruit Yield and Quality and Blossom End Rot Control Using Different Biological Calcium Sources

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Journal of Agricultural Chemistry and Environment 2023年第3期12卷 263-274页

作者： Alain Serge Coulibaly Kouakou Laurent Kouakou jonas Patrick Dao Camille Kouakou Juliette Ky Dedi Irié Arsène Zoro Bi Training and Research Unit of Sciences of Nature Laboratory of Biology and Improvement of Plant Production Nangui Abrogoua University Abidjan C&ocircte d’Ivoire Phytotechnical Unit and Genetic Improvement Training and Research Unit of Sciences of Nature Nangui Abrogoua University Abidjan C&ocircte d’Ivoire Training and Research Unit of Biosciences Plant Physiology Laboratory Felix Houphou&eumlt Boigny University Abidjan C&ocircte d’Ivoire

Cultivated for its richness in nutrients, tomato culture is demanding fertilizer, especially in calcium. Calcium deficiencies and physiological disorders cause serious diseases in tomatoes. Blossom end rot (BER), in susceptible cultivars, may cause severe economic losses. The goal of this study is to determine the effect of three organic calcium sources on the growth and quality of fruit production of tomatoes. Egg, snail and sea shell powders and extracts have been used as biological fertilizers. The experiment was conducted in a block factorial trial with three replications. The trials comprised two factors: calcium sources and calcium source form (powder and extract). The results showed that the growth parameters and yield were improved by the calcium source, whatever the form. Also, BER was lower in plants treated with calcium contrary to the control. However, plants treated with eggshells showed the best growth (mean) and the lowest rate (0.40%) of BER than the snail and sea shell.

关键词： Tomatoes Blossom End Rot Eggshells Snail Shells Seashells

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Evaluation of Glutathione Peroxidase Enzymatic Activity in Seminal Plasma of Patients Treated at the Institute Pasteur in Cote d’Ivoire

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Advances in Reproductive Sciences 2023年第4期11卷 116-126页

作者： Marie Florence N’Guessan Bi Bali Sery Foua jonas Vanié Bi N’Gbesso Amos Ekissi Youzan Ferdinand Djohan Founzégué Amadou Coulibaly Allico Joseph Djaman Biochemistry Unit Physiology of Reproductive Cells Institute Pasteur in Ivory Coast Abidjan Cote d’Ivoire The Laboratory of The Medical Sciences Training and Research Unit Felix Houphouet-Boigny University Abidjan Cote d’Ivoire Medical Sciences Training and Research Unit Department of Basic and Bioclinical Sciences Abidjan Felix Houphouet-Boigny Uni-versity Abidjan Cote d’Ivoire Laboratory of Biotechnology Agriculture and Valorization of Biological Resources Departement Biosciences University Félix Hou-phouet-Boigny Abidjan Cote d’Ivoire Agriculture Fishery Resources and Agro-Industry Training and Research Unit University of San-Pedro San-Pedro Cote d’Ivoire Departement of Clinical and Fundamental Biochemistry Institute Pasteur in Cote d’Ivoire Abidjan Cote d’Ivoire

Glutathione peroxidase (GPx) is an antioxidant that plays an important role in the maintenance of male fertility. The aim of this study was to compare the profile of enzymatic activity of glutathione peroxidase in the seminal plasma of normozoosperm and those of pathological sperm. Thus, the activity of glutathione peroxidase was determined in the seminal plasma of 20 normozoosperms, 9 azoosperms and 31 oligoasthenoteratozoosperms. It was 37.58 ± 3.14 U/L in normozoosperms, 39.39 ± 2.27 U/L in oligoasthenoteratozoosperms, and 29.77 ± 2.62 U/L in azoosperms. The mean GPx enzyme activity of normozoosperms did not differ significantly from that of oligoasthenoteratozoosperms and azoosperms. In contrast, comparison of enzyme activity between abnormal sperms gave a significant difference. This study showed that glutathione peroxidase enzymatic activity is not related to sperm quality.

关键词： Glutathione Peroxidase Antioxidant Oligoasthenoteratozoospermia Azoospermia Normozoospermia

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Characterization of Two Clay Raw Materials from Côte d’Ivoire with a View to Enhancing Them in Eco-Construction

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Journal of Minerals and Materials Characterization and Engineering 2022年第2期10卷 198-208页

作者： Lébé Prisca M.-S. Kouakou Alfred Niamien Kouamé Bi Irié Hervé Gouré Doubi Namory Méité Jean Thierry Kangah Esmel Privat Zokou Léon Koffi Konan Yao jonas Andji-Yapi Laboratory of Constitution and Reaction of Matter University Felix Houphouet Boigny Abidjan Cote d’Ivoire Unit for Training and Research of Biological Sciences University Peleforo Gon Coulibaly Korhogo Cote d’Ivoire

This study was carried out with a view to appreciate the value of clay, raw materials in eco-construction. To achieve this, we sampled two clay raw materials denoted Aga and Bak and then characterized. The results obtained from geotechnical and mineralogical tests have shown that the clay samples Aga and Bak are fine soils moderately plastic class A soils consisting essentially of quartz with 73.13% and 74.56% respectively for Aga and Bak and clay minerals (kaolinite and illite) with 12.73% kaolinite and 8.55% illite for Aga against 8.31% kaolinite and 13.72% for Bak. Moreover, these samples do not contain swelling clays and contain a sufficient quantity of iron oxides which allows them to be valued in ceramics, in particular in compressed earth bricks (CEB).

关键词： Clays Compressed Earth Bricks Eco-Material Physico-Chemical and Geotechnical Properties

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Effect of Leafy and Leafless Greenwood, Softwood and Hardwood Cuttings Success of <i>Garcinia kola</i>(Heckel)

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Agricultural Sciences 2020年第10期11卷 897-911页

作者： jonas Patrick Dao Kouakou Laurent Kouakou Camille Kouakou Mamadou Cherif Mahamadi Hamed Ouedraogo Kouame Kevin Koffi Irié Arsène Zoro Bi Training and Research Unit of Sciences of Nature Laboratory of Biology and Improvement of Plant Production Nangui Abrogoua University Abidjan C&#244 te d’Ivoire Training and Research Unit of Biosciences Plant Physiology Laboratory Felix Houphou&#235 t Boigny University Abidjan C&#244 te d’Ivoire Phytotechnical Unit and Genetic Improvement Training and Research Unit of Sciences of Nature Nangui Abrogoua University Abidjan C&#244 te d’Ivoire Training and Research Unit of Life and Earth Sciences Biosciences Laboratory Genetics and Plant Breeding Joseph Ki-Zerbo University Ouagadougou Burkina Faso

Garcinia kola Heckel, called “petit cola” in Côte d’Ivoire and “bitter kola” in Nigeria, is a Non-Timber Forest Product of great socioeconomic importance. Unfortunately, this species is threatened due to overexploitation and deforestation. This study tested the effect of leaf area on stem cuttings to regenerate vegetatively in a non-mist poly-propagator without using exogenous hormones. Three stem cuttings types: greenwood, softwood and hardwood (12 - 15 cm in length) were collected from 24-month years old seedlings. The two top leaves of each cutting type were conserved at different leaf area: 0 cm2, 28.25 cm2, 56.5 cm2, 84.75 cm2 and 113 cm2 (entire leaf area). Observations were made on rooting, sprouting and leafing abilities of cuttings depending to treatments applied. Results showed that all cutting types have presented good ability to shooting and rooting. Cuttings with 113 cm2 leaf areas presented the best rooting (94.44% ± 2.42%) and shouting (95.55% ± 2.42%) percentage and longest primary root length (12.03 ± 0.50 cm). Hardwood, softwood and greenwood cuttings with 113 cm2 leaf area had the best rooting percentage (96.66% ± 3.33%, 96.66% ± 5.77% and 90.00% ± 5.77% respectively). The longest primary root length (13.50 ± 0.97 cm) was observed to greenwood cuttings with 113 cm2 leaf area. Regenerated plants in the forest presented well survival percentage (96.66%). This study shows that it is possible to regenerate entire plants by cuttings without using external hormones in non-mist poly-propagator.

关键词： Garcinia kola Heckel Leaf Area Non-Mist Poly-Propagator Stem Cuttings Vegetative Propagation

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