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Clinical and Radiographic Evaluation of Cattle Tail before and after the <i>Vaquejada</i>Race

Open Journal of Veterinary Medicine 2021年第6期11卷 165-176页

作者： Raíssa Karolliny Salgueiro Cruz Maria Luiza Albuquerque Ribeiro Juliano Sarmento Macedo Brunno Aguiar Ferreira Marcelo Araújo Silva Anaemília das neves Diniz Helena Emília Cavalcanti da Costa Cordeiro Manso Helio Cordeiro Manso Filho Professor of the Veterinary Medicine course Cesmac University Center (CESMAC) Maceió-AL Brazil Veterinarian and Member of the Association of Veterinarians of Equidae and Bovids of Alagoas (AMVEBAL) Maceió-AL Brazil Adjunct Professor at the Federal University of Alagoas Maceió-AL Brazil Equine Research Center Federal Rural University of Pernambuco Recife-PE Brazil

The vaquejada race is a sport with animals which has the greatest socio-economic impact in Brazil and is responsible for generating an intense cultural and economic movement. However, this activity has been the subject of questions about well-being involving the environmental and mental comfort conditions offered to equine and cattle interactions. It is necessary to perform technical evaluations of the cattle involved on the basis of scientific study to better understand the processes of sport and, with this, promote their evolution on good welfare practices in the 21st century. Therefore, to test the hypothesis that the use of the official rules of the vaquejada where cattle use a protective harness on the tail protects the cattle’s tail from injuries in this region that compromise health. Before, during and after races, a study was developed that aimed to perform a general and radiological clinical evaluation in the proximal coccygeal region in cattle that ran in a modern cattle stall. Forty male bovines were evaluated (average: 2 year-old and 420 kg). Oxen were subjected to clinical and radiological examination before and after racing with official rules. Results of the clinical and radiological evaluations performed by veterinarians, did not observe clinical occurrences before and after the races in the 40 cattle evaluated. The increase in heart and respiratory rates was a reflection of temporary management stressors, as the animals returned to the feeding/rumination/interaction pattern after running in the rest pen. The radiographic findings in the tail, represented by the reduction of the intervertebral space and areas of subcutaneous radiolucency, suggest technical artifacts derived from

关键词： Zebu Physical Exercise Locomotor Health

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Detection of Contamination and Analysis of Vertical Transmission of BmNPV in Eggs and Moths of Bombyx mori

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Open Journal of Genetics 2014年第5期4卷 370-377页

作者： Claudia Regina das neves Saez Roxelle Ethienne Ferreira Munhoz Naiara Climas Pereira Thais Souto Bignotto Veronica Aureliana Fassina Graziele Milani Pessini Laura Beatriz Garay Lucineia de Fatima Chasko Ribeiro Rose Meire Costa Brancalhao Maria Aparecida Fernandez Departamento de Biotecnologia Genetica e Biologia CelularUniversidade Estadual de MaringaMaringaBrasil Seda y Fibras HernandariasParaguay Universidade Estadual do Oeste do Parana CascavelBrasil

This study reports the molecular detection of Bombyx mori nucleopolyhedrovirus (BmNPV) in silkworm strains of the Universidade Estadual de Maringá Brazilian Germplasm Bank (UBGB). DNA extraction was carried out by using six Bombyx mori female moths of each strain, followed by PCR amplification. A pair of primers was designed based on a specific sequence of the baculovirus genome related to the BmNPV ORF 14. Another pair of primers was used to amplify the silkworm Actin A3 gene segment, which was used as positive control. Twenty gene pools were analyzed, and fifteen revealed a fragment of 443 base pairs (bp), which indicated the presence of the BmNPV. The frequency of contaminated moths was as following: 100% for silkworm strains M18-2, M12-2 and J1;83% for C25, C75 and C24 strains;66% for KR01;50% for M11-A;33% for AS3, B106, M8 and M11 and 16% for C211, E8 and Hindu strains. These are promising results for the identification of contaminated B. mori moths by BmNPV, which may prevent virus proliferation in subsequent generations. We also analyzed DNA samples extracted from B. mori eggs, but the results were not conclusive regarding the detection of the fragments of the expected size (443 bp). The difficulty in detecting BmNPV contamination in B. mori eggs may be due to the low concentration of virus in samples.

关键词： Silkworm Diseases Sericulture Viruses in Insects BmNPV Bombyx mori

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Carbon Burial in Young Tropical Reservoirs Is Higher at Lower Latitudes*

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Journal of Geoscience and Environment Protection 2021年第12期9卷 84-99页

作者： Elizabeth Sikar Marco Aurelio dos Santos Ana Carolina Moterani Isabella Matvienko Ana Carla Martins Veras Juliana Pinto Pereira Dias Orleno Marques da Silva Junior Claudio Pavani Marcelo Amorim Teodosio das neves Milisse Nzualo Ayr Manoel Portilho Bentes Junior Rogerio Duarte Construmaq Sã o Carlos S&#227 o Carlos SP Brazil Programa de Planejamento Energético da Universidade Federal do Rio de Janeiro Brazil Laboratório de Energias Renováveis e Estudos Ambientais da Universidade Federal do Rio de Janeiro Brazil Instituto de Pesquisas Científicas e Tecnológicas do Estado do Amapá Brazil Programa de Pós-Graduaç ã o em Geografia da Universidade Federal do Amapá Brazil

Man-made environments such as tropical hydroelectric reservoirs alter the preexisting carbon (C) cycle and remove C from circulation through burial in sediments. Carbon burial (CB) was measured using the silica-tracer method during four field surveys in the less than six-year-old Belo Monte tropical reservoir. Fresh C sedimentation was also measured. Belo Monte’s CB median rate 276 (n = 84;min 0;max 352,625 mg C·m-2·d-1) is within the range (230 to 436 mg C·m-2·d-1) of CB rates measured further downstream at the Xingu Ria and higher than the averaged over 50 years oceanic rate 244 mg C·m-2·d

关键词： Carbon Burial Sediments Hydroelectric Reservoirs

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Antitumor effects of electrochemical treatment

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Chinese Journal of Cancer Research 2013年第2期25卷 223-234页

作者： Héctor Manuel Camué Ciria Maraelys Morales González Lisset Ortíz Zamora Luis Enrique Bergues Cabrales Gustavo Victoriano Sierra González Luciana Oliveira de Oliveira Rodrigo Zanella Antonio Carlos Buzaid Orlando Parise Luciana Macedo Brito Cesar Augusto Antunes Teixeira Marina das neves Gomes Gleyce Moreno Venicio Feo da Veiga Marcos Telló Carla Holandino Research Department National Centre of Applied Electromagnetism Oriente University Pharmacy Department Oriente University Finlay Institute La lisa. La Habana Cuba Veterinary Teaching Hospital Federal University of Rio Grande do Sul. Porto Alegre Sírio e Libanês Hospital Faculty of Pharmacy Medicine Department Federal University of Rio de Janeiro Professor Paulo de Góes Institute of Microbiology Federal University of Rio de Janeiro Pontifical Catholic University of Rio Grande do Sul. Porto Alegre

Electrochemical treatment is an alternative modality for tumor treatment based on the application of a low intensity direct electric current to the tumor tissue through two or more platinum electrodes placed within the tumor zone or in the surrounding areas. This treatment is noted for its great effectiveness, minimal invasiveness and local effect. Several studies have been conducted worldwide to evaluate the anfitumoral effect of this therapy. In all these studies a variety of biochemical and physiological responses of tumors to the applied treatment have been obtained. By this reason, researchers have suggested various mechanisms to explain how direct electric current destroys tumor ceils. Although, it is generally accepted this treatment induces electrolysis, electroosmosis and electroporation in tumoral tissues. However, action mechanism of this alternative modality on the tumor tissue is not well understood. Although the principle of Electrochemical treatment is simple, a standardized method is not yet available. The mechanism by which Electrochemical treatment affects tumor growth and survival may represent more complex process. The present work analyzes the latest and most important research done on the electrochemical treatment of tumors. We conclude with our point of view about the destruction mechanism features of this alternative therapy. Also, we suggest some mechanisms and strategies from the thermodynamic point of view for this therapy. In the area of Electrochemical treatment of cancer this tool has been exploited very little and much work remains to be done. Electrochemical treatment constitutes a good therapeutic option for patients that have failed the conventional oncology methods.

关键词： Direct electric current electrochemical treatment antimmoral treatment tumor tumor electrolysis rumor entropy dissipative structure

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Evaluation of the toxic effect of insecticide chlorantraniliprole on the silkworm Bombyx mori(Lepidoptera:Bombycidae)

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Open Journal of Animal Sciences 2013年第4期3卷 343-353页

作者： Roxelle Ethienne Ferreira Munhoz Thais Souto Bignotto Naiara Climas Pereira Claudia Regina das neves Saez Rafaela Bespalhuk Veronica Aureliana Fassina Graziele Milani Pessini Mayarha Patricia Dequigiovanni Baggio Lucineia de Fatima Chasko Ribeiro Rose Meire Costa Brancalhao Shunsuke Mizuno Willian Shigeaki Aita Maria Aparecida Fernandez Departamento de Biotecnologia Genetica e Biologia CelularUniversidade Estadual de MaringaMaringaBrasil Universidade Estadual do Oeste do Parana CascavelBrasil Fiacao de Seda Bratac S.A.Bastos Sao PauloBrasil

The silkworm Bombyx mori feeds exclusively on mulberry leaves and is highly sensitive to pesticides in general. Although mulberry plantations are free of agrochemicals, pesticide drift can occur. Chlorantraniliprole, a novel insecticide of the anthranilic diamides class, has been used to control pests in field crops. In this study, we investigated the biological effects of different concentrations of chlorantraniliprole on B. mori silkworm commercial Brazilian hybrids. To evaluate the toxicity of chlorantraniliprole, bioassays were carried out and data on the lethal concentrations, symptomatology, morphology and variables of silk production were collected. Results indicated that B. mori is extremely sensitive to chlorantraniliprole, even in low concentrations. The highest silkworm mortality rates were observed in the two highest chlorantraniliprole concentrations, 0.2 and 0.1 ppm. Although lower chlorantraniliprole concentrations did not cause death of all the silkworm larvae, various symptoms of toxicity were observed: feeding cessation, regurgitation, late development and incomplete ecdysis. Such symptoms reflect the morphological changes we observed in the midgut epithelium, which affected nutrient uptake and metabolism, and even the production of cocoons. Exposed larvae also produced?thin-shelled cocoons, which constitutes a serious economic problem because this type of cocoon is not useful for the silk industry. The results provided herein confirm the toxicity of chlorantraniliprole in silkworm larvae. Therefore, we strongly suggest that, competent authorities of the National Health Surveillance Agency, in pesticide management should take measures to reduce or eliminate the use of chlorantraniliprole in areas nearest to silkworm cultivation.

关键词： Silkworm Pesticide Drift Mortality Symptomatology Chlorantraniliprole

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根管内增强纤维在儿科的应用：病例报告

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中国口腔医学继续教育杂志 2005年第3期8卷 56-58页

作者： Rachel de Oliveira Rocha Lucimara Teixeira das neves Noely Regina Marotti Marcia Turolla Wanderley Maria Salete Nahás Pires Corre^a 吴南(译) 赵玉鸣(校) 不详北京大学口腔医学院北京海淀区中关村南大街22号100081

本文介绍了一种使用间接树脂冠和根管内增强纤维来修复龋坏的上颌乳切牙的方法。每颗牙齿都先进行了根管治疗。使用根管内增强纤维的优点包括：树脂冠强度增加，透明，相对操作简便。另外，使用间接树脂冠能够获得很好的外形和美观，同... 详细信息

本文介绍了一种使用间接树脂冠和根管内增强纤维来修复龋坏的上颌乳切牙的方法。每颗牙齿都先进行了根管治疗。使用根管内增强纤维的优点包括：树脂冠强度增加，透明，相对操作简便。另外，使用间接树脂冠能够获得很好的外形和美观，同时能够减少儿童的椅旁时间。间接树脂冠和根管内增强纤维在一名3岁女孩的使用以图示说明这一技术。

关键词：根管增强纤维儿科上颌乳切牙牙齿

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Prevalence of Kudoa in Fish Fillets Caught in Para State

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Journal of Zoological Research 2020年第3期2卷 9-14页

作者： Marianna Vaz RODRIGUES Patrícia Tidori MIURA Jéssica Fernandes de OLIVEIRA Maria das Dores Correia Palha João Pessoa Araújo JÚNIOR UNESP-São Paulo State University(Unesp) BiotechnologyAlameda das Tecomarias s/n18607-440BotucatuSão PauloBrazil Food Technology Laboratory Food Science DepartmentUniversity of CampinasCidade Universitária“Zeferino Vaz”s/n13083-862CampinasSão PauloBrazil Universidade Federal Rural da Amazônia Avenida Presidente Tancredo Neves 250166077-830BelémParáBrazil

Kudoa is a myxozoan that causes myoliquefaction in marine *** of species only affect fish,but a *** outbreak was reported in 358 *** many species of Kudoa are known,none was described in Brachyplatystoma filamentosum,Brachyplatystoma rousseauxii,Mugil curema,Plagioscion squamosissimus or Oxydoras niger until *** to the economic cost of eliminating seafood presenting myxozoan lesions,this study aimed to describe lesions found at necropsy and histopathology,as well as to detect this myxozoan by molecular *** this purpose,were sampled 85 fish of the following species:Brachyplatystoma filamentosum,Brachyplatystoma rousseauxii,Mugil curema,Plagioscion squamosissimus,and Oxydoras niger from Colares and Vigia,Pará,*** were carried out to describe lesions and molecular techniques(PCR and sequencing)were applied for *** muscle lesions were not observed at necropsy,histopathology revealed bacterial colonies,coagulative necrosis,dystrophic calcification,eosinophils,hemorrhage,parasitic pseudocysts,protozoan,and *** sequencing,***(GENBANK:LC128646)was identified as the most similar causative agent of fishes infection,but due to phylogenetic results and identity we suggest that the myxozoan found could be a new ***,high parasitism of this myxozoan was observed in fishes sampled,i.e.,90%in Colares and 100%in Vigia.

关键词： Myxozoan Parasitism Seafood Sanitary inspection Myoliquefaction

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