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Food Science and Human Wellness 2024年

作者： Qiang Wu feijun Luo Jia Huang Ping Huang Yongnan Liu Xiaoling wang Gaoqiang Liu Hunan Provincial Key Laboratory of Forestry Biotechnology and International Cooperation Base of Science and Technology Innovation on Forest Resource Biotechnology Central South University of Forestry and Technology Microbial Variety Creation Center Laboratory of Yuelushan Seed Industry College of Food and Chemical Engineering Shaoyang University Laboratory of Molecular Nutrition National Engineering Laboratory for Rice and Byproducts Central South University of Forestry and Technology

Ganoderma lingzhi is a new species of the prize medicinal mushroom Ganoderma (Agaricomycetes). Using angiotensin I-converting enzyme (ACE) as a target， a tripeptide Ser-Tyr-Pro (SYP) was discovered with preponderant ACE inhibitory activity with an 50% inhibiting concentration (IC50) value of 62.50 μg/mL attribute to the formed salt bridge and hydrogen bonds between SYP and ACE. SYP even maintained superior bioactivity after intestinal digestion， and exerted no cytotoxicity， but presented incomplete bioavailability in blood of spontaneous hypertensive rats (SHRs). Furthermore， it performed antihypertensive effect in vivo by inhibiting the influx of Ca2+through activating endothelial NO synthase (eNOS)/NO/guanosine 3’，5’-cyclic monophosphate (cGMP) pathway， accompanied by attenuating angiotensin Ⅱ (Ang Ⅱ)/NADPH oxidase (NOX)/ROS pathway. This work not only discoverers a novel pharmacological ingredient from medicinal mushroom G. lingzhi for hypertension therapy， but also provides an insight into molecular mechanism of the ACE inhibitory peptide (ACEIP) on lowering blood pressure.

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Identification and fine mapping of rtms1-D,a gene responsible for reverse thermosensitive genic male sterility from Diannong S-1X

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Plant Diversity 2022年第2期44卷 213-221页

作者： Xiao Zhang Guimei Chang Zihao Wu Jinpeng Wan Jun Yang feijun wang Fang wang Diqiu Yu Peng Xu CAS Key Laboratory of Tropical Plant Resources and Sustainable Use Xishuangbanna Tropical Botanical GardenChinese Academy of SciencesMenglunMenglaYunnan 666303China College of Life Sciences University of Chinese Academy of SciencesBeijing 100049China Center of Economic Botany Core Botanical GardensChinese Academy of SciencesMenglunMenglaYunnan 666303China The Innovative Academy of Seed Design Chinese Academy of SciencesMenglunMenglaYunnan 666303China

Thermosensitive genic male sterility(TGMS)has been widely used in two-line hybrid rice breeding.Due to hybrid seed production being highly affected by changeable environments,its application scope is limited to some extent.Thus,it is of great importance to identify potential TGMS genes in specific rice varieties.Here,Diannong S-1 xuan(DNS-1 X),a reverse TGMS(RTGMS)japonica male sterile line,was identified from Diannong S-1.Genetic analysis showed that male sterility was tightly controlled by a single recessive gene,which was supported by the phenotype of the F;and F;populations derived from the cross between DNS-1 X and Yunjing 26(YJ26).Combining simple sequence repeat(SSR)markers and bulked segregation analysis(BSA),we identified a 215 kb region on chromosome 10 as a candidate reverse TGMS region,which was designated as rtmsl-D.It was narrower than the previously reported RTGMS genes rtmsl and tms6(t).The fertility conversion detected in the natural environment showed that DNS-1 X was sterile below 28-30℃;otherwise,it was fertile.Histological analysis further indicated that the pollen abortion was occurred in the young microspore stage.This study will provide new resources for two-line hybrid rice and pave the way for molecular breeding of RTGMS lines.

关键词： Rice(Oryza sativa L) Reverse thermosensitive genic male sterile (RTGMS) rtmsl-D Fertility transition

Natural alleles of a uridine 5'-diphospho-glucosyltransferase gene responsible for differential endosperm development between upland rice and paddy rice

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Journal of Integrative Plant Biology 2022年第1期64卷 135-148页

作者： Zihao Wu Xiao Zhang Guimei Chang Jun Yang Jinpeng Wan feijun wang Dayun Tao Jiawu Zhou Lianguang Shang Peng Xu Diqiu Yu School of Life Sciences University of Science and Technology of ChinaHefei 230027China CAS Key Laboratory of Tropical Plant Resources and Sustainable Use Xishuangbanna Tropical Botanical Gardenthe Chinese Academy of SciencesKunming 650223China Yunnan Key Laboratory for Rice Genetic Improvement Food Crops Research InstituteYunnan Academy of Agricultural SciencesKunming 650200China The Innovative Academy of Seed Design Chinese Academy of SciencesMengla 666303China Shenzhen Branch Guangdong Laboratory of Lingnan Modern Agricultural and Rural AffairsAgricultural Genomics Institute at ShenzhenChinese Academy of Agricultural SciencesShenzhen 518000China Center of Economic Botany Core Botanical GardensChinese Academy of SciencesMergla 666303China State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan Yunnan UniversityKunming 650091China

Traditional upland rice generally exhibits insufficient grains resulting from abnormal endosperm development compared to paddy rice. However, the underlying molecular mechanism of this trait is poorly understood. Here,we cloned the uridine 5’-diphospho(UDP)-glucosyltransferase gene EDR1(Endosperm Development in Rice) responsible for differential endosperm development between upland rice and paddy rice by performing quantitative trait loci analysis and map-based cloning. EDR1 was highly expressed in developing seeds duringgrain filling. Natural variations in EDR1 significantly reduced the UDP-glucosyltransferase activity of EDR1 YZNcompared to EDR1 YD1,resulting in abnormal endosperm development in the near-isogenic line, accompanied by insufficient grains and changes in grain quality.By analyzing the distribution of the two alleles EDR1 YD1 and EDR1 YZNamong diverse paddy rice and upland rice varieties, we discovered that EDR1 was conserved in upland rice, but segregated in paddy rice. Further analyses of grain chalkiness in the alleles of EDR1 YD1 and EDR1 YZNvarieties indicated that rice varieties harboring EDR1 YZNand EDR1 YD1 preferentially showed high chalkiness, and low chalkiness,respectively. Taken together, these results suggest that the UDP-glucosyltransferase gene EDR1 is an important determinant controlling differential endosperm development between upland rice and paddy rice.

关键词： EDR1 endosperm development grain quality UDP-glucosyltransferase upland rice

基于溶液除湿的新型粮食干燥设备设计及特性分析

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食品与发酵工业 2024年第5期50卷 290-296页

作者：闫俊海高龙刘寅丁扬铮孟照峰王顺吴飞骏谢亚琦张士雯中原工学院能源与环境学院河南郑州450007

我国每年因粮食霉变等原因造成的损失巨大,粮食干燥是实现安全储藏的重要环节之一。另外,随着对粮食品质要求的不断提高,低温通风除湿干燥成为一个主要发展方向。溶液除湿技术相比于冷凝法去湿,具有除湿效果好、温湿度控制精确、节能等... 详细信息

我国每年因粮食霉变等原因造成的损失巨大,粮食干燥是实现安全储藏的重要环节之一。另外,随着对粮食品质要求的不断提高,低温通风除湿干燥成为一个主要发展方向。溶液除湿技术相比于冷凝法去湿,具有除湿效果好、温湿度控制精确、节能等优势,应用日益广泛。该设计以“绿色、健康、生态”粮食储藏为理念,开发了基于溶液除湿的新型粮食干燥设备,并通过实验,对机组性能进行了分析。实验证明,不同环境空气工况下,机组出口空气参数达到了设计要求。该系统以电能驱动,并利用冷凝废热驱动溶液循环,系统制冷性能系数(coefficient of refrigeration performance,COPC)可达4.20以上、除湿性能系数(dehumidification coefficient,COPD)不低于3.1,与冷凝法低温干燥机相比可节能50%以上,与烘干机相比可节能20%左右,节能效果显著。从环保、储粮品质的提升、系统节能考虑,该设备具有较大优势,市场应用前景广阔。

关键词：粮食低温慢速干燥就仓干燥溶液除湿

23价肺炎球菌多糖疫苗在≥2岁健康人群中安全性和免疫原性Ⅳ期临床试验评价

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中国疫苗和免疫 2024年第2期30卷 184-190页

作者：钱小爱刘凯杨北方王硕柯华柯昌显李锦霞张治国穆飞军张馨月周婷婷童叶青官旭华魏晟郑景山张险峰湖北省疾病预防控制中心湖北武汉430079 北京民海生物科技有限公司北京102600 十堰市郧阳区疾病预防控制中心湖北十堰442500 荆州市监利市疾病预防控制中心湖北荆州433300 华中科技大学同济医学院公共卫生学院湖北武汉430030

目的评价≥2岁健康人群接种23价肺炎球菌多糖疫苗(Pneumococcal polysaccharide vaccine,PPV23)的安全性和免疫原性。方法采用开放性Ⅳ期临床试验,招募2-5岁、6-15岁、16-59岁和≥60岁健康受试者,观察1剂次PPV23接种后30 d内不良事件,... 详细信息

目的评价≥2岁健康人群接种23价肺炎球菌多糖疫苗(Pneumococcal polysaccharide vaccine,PPV23)的安全性和免疫原性。方法采用开放性Ⅳ期临床试验,招募2-5岁、6-15岁、16-59岁和≥60岁健康受试者,观察1剂次PPV23接种后30 d内不良事件,分析发生率;检测接种前和接种后30 d血清标本23种肺炎链球菌(Streptococcus pneumoniae,Spn)血清型IgG抗体,评价接种后抗体阳转率、几何平均浓度(Geometric mean concentration,GMC)和几何平均增长倍数(Geometric mean increase,GMI)。结果在2997例受试者中,PPV23接种后与疫苗有关的不良事件发生率为10.84%(325例),其中1级、2级、3级、4级不良事件分别为9.94%、0.77%、0.13%、0.00%;接种部位疼痛、发热分别为4.50%、1.40%。在273例受试者中,PPV23接种后23种Spn血清型IgG抗体阳转率在92.83%-99.66%之间,GMC在2.00-63.34μg/mL之间,GMI在6.82-171.24之间。结论≥2岁健康人群接种1剂次PPV23具有良好的安全性和免疫原性。

关键词： 23价肺炎球菌多糖疫苗安全性免疫原性 Ⅳ期临床试验

Electrospinning of hydroxypropyl methylcellulose trimellitate solutions

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Science China Chemistry 2010年第1期53卷 190-195页

作者： MA Gang,SHAO ZiQiang,wang WenJun,wang feijun,TAN LingLing & LIAO Bing School of Material,Beijing Institute of Technology,Beijing 100081,China School of Material Beijing Institute of Technology Beijing China

Nonwoven fiber mats of hydroxypropyl methylcellulose trimellitate(HPMCT) with potential applications in controlled delivery of drugs and scaffolds for tissue cultures have been successfully fabricated by electrospinning of HPMCT solutions.The formation and diameters of HPMCT fibers fabricated by electrospinning were strongly influenced by the solvents employed,electrostatic field strength,and solution concentrations.The electrospun products generated from all HPMCT solutions with various weight-average molecular weights in methanol/dichloromethane(1:1) solvent consisted of clustered beads,while methanol/dichloromethane(1:5) solvent helped to generate smooth,uniform,submicron fibers.From the relationship between specific viscosity and molecular weight in different solvents,it could be concluded that methanol/dichloromethane(1:5) solvent enhanced the molecular entanglements,improved the viscosity of the solutions,thus helped to generate smooth fibers.When all the other variables were kept constant,the concentration range suitable for electrospinning was between 10% and 16%(wt),and the average fiber diameter increased as the concentration increased.When all the other variables were kept constant,the average fiber diameter decreased as the strength of the electrostatic field increased.Electrospinning the solutions of two compounds with similar structure,hydroxypropyl methylcellulose phthalate(HPMCP) and HPMCT,we found that the fibers electrospun from HPMCP were thicker than that from HPMCT,which is attributed to the fact that the solution's conductivity of HPMCT(containing two free carboxyl groups) is higher than that of HPMCP(containing only one free carboxyl group).

关键词： electrospinning HPMCT ultrafine fibers structure properties

腹腔镜结直肠癌手术采用围术期多模式积极体温保护措施40例效果探讨

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中国基层医药 2023年第7期30卷 1070-1073页

作者：陈海飞郑双王飞筠台州市第一人民医院麻醉手术科台州318020

目的探讨腹腔镜结直肠癌手术患者采用围术期多模式积极体温保护措施效果。方法选取台州市第一人民医院2021年8月至2022年3月收治的腹腔镜结直肠癌根治术患者80例为研究对象,采用随机数字表分法为对照组和观察组,每组40例。对照组围术期... 详细信息

目的探讨腹腔镜结直肠癌手术患者采用围术期多模式积极体温保护措施效果。方法选取台州市第一人民医院2021年8月至2022年3月收治的腹腔镜结直肠癌根治术患者80例为研究对象,采用随机数字表分法为对照组和观察组,每组40例。对照组围术期接受常规体温管理,观察组接受多模式积极体温保护措施。比较两组不同时间点鼻咽温度、手术指标、中性粒细胞绝对值与淋巴细胞绝对值之比(NLR)、血小板计算与淋巴细胞绝对值之比(PLR)以及术后不良反应情况。结果观察组手术前0.5 h温度[(36.32±0.58)℃]、手术开始1 h温度[(36.24±0.25)℃]、手术结束时温度[(36.17±0.33)℃]均显著高于对照组[(35.73±0.64)℃、(35.51±0.27)℃、(35.84±0.57)℃](F=11.48,P < 0.05)。观察组术中出血量[(27.58±1.96)mL]、苏醒时间[(26.28±2.36)min]以及肠道恢复时间[(19.21±2.01)h]均显著低于对照组[(38.21±0.65)mL、(49.23±4.52)min、(31.19±2.38)h](t=32.56、28.47、24.32,均P < 0.05)。手术后,观察组NLR(6.39±0.49)、PLR(225.08±29.21)均显著低于对照组[(11.74±1.32)、(325.42±34.36)](t=24.03、14.07,均P < 0.05)。观察组手术前后NLR差值(4.01±0.52)、PLR差值(76.93±22.18)均显著低于对照组[(9.04±0.98)、(176.42±29.47)](t=28.68、17.06,均P < 0.05)。观察组术后寒战发生率[5.00%(2/40)]显著低于对照组[27.50%(11/40)](χ^(2)=5.88,P=0.015)。结论围术期多模式积极体温保护措施有助于改善腹腔镜结直肠癌手术患者体温,加速术后恢复,减轻患者全身炎症反应,降低术后寒战发生率,值得临床推广。该研究具备显著创新性和科学性。

关键词：结直肠肿瘤腹腔镜检查围手术期体温不良反应炎症反应失血,手术麻醉苏醒治疗结果

高分辨质谱法和核磁共振法定性分析合成阿片类新精神活性物质2-甲基-布桂嗪

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理化检验（化学分册） 2023年第1期59卷 13-20页

作者：何思阳王跨陡邓乾亚汪蓉梁晨龚飞君上海市公安局物证鉴定中心上海市现场物证重点实验室上海200083

利用气相色谱-四极杆飞行时间质谱法(GC-Q-TOF MS)、高效液相色谱-线性离子阱/静电场轨道阱质谱法(HPLC-LTQ/Orbitrap MS)和核磁共振法(NMR)对缴获的未知样品进行定性分析。样品经甲醇溶解,涡旋、离心,取上清液供GC-Q-TOF MS检测,保留... 详细信息

利用气相色谱-四极杆飞行时间质谱法(GC-Q-TOF MS)、高效液相色谱-线性离子阱/静电场轨道阱质谱法(HPLC-LTQ/Orbitrap MS)和核磁共振法(NMR)对缴获的未知样品进行定性分析。样品经甲醇溶解,涡旋、离心,取上清液供GC-Q-TOF MS检测,保留时间为27.92 min组分的质谱碎片主要特征离子质荷比(m/z)为286.203 7,215.154 0,195.149 0,172.111 8,117.069 6(基峰)。经HPLC-LTQ/Orbitrap MS分析,质子化分子离子m/z为287.212 1。对比国家管制麻醉药品布桂嗪的相应质谱信息[主要特征离子m/z为272.188 1,201.138 4,181.133 3,172.111 8,117.069 6(基峰),质子化分子离子m/z为273.196 5],推测未知样品为布桂嗪的甲基衍生物,分子式为C_(18)H_(26)N_(2)O。经NMR分析,确认该未知样品为合成阿片类新精神活性物质2-甲基-布桂嗪。

关键词：高分辨质谱法核磁共振法布桂嗪 2-甲基-布桂嗪

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岩溶塌陷时管土相互作用准静态部分模拟研究

人民长江 2022年第12期53卷 96-103页

作者：刘国栋沈飞军王晓欣肖术长江三峡勘测研究院有限公司(武汉) 湖北武汉430074 国家管网西气东输武汉管理处湖北武汉430074

为预估岩溶塌陷时输油、气、水管道的附加力,进而指导防护结构设计,基于牛顿第三运动定律,在不考虑动力冲击条件下采用颗粒离散元法,模拟了土洞塌陷时管土相互作用的准静态过程,获取了管道的附加力。探讨了仿真结果的离散性和尺寸效应问... 详细信息

为预估岩溶塌陷时输油、气、水管道的附加力,进而指导防护结构设计,基于牛顿第三运动定律,在不考虑动力冲击条件下采用颗粒离散元法,模拟了土洞塌陷时管土相互作用的准静态过程,获取了管道的附加力。探讨了仿真结果的离散性和尺寸效应问题,揭示了由于土洞塌陷导致的管道最大附加力与其主要影响因素(管径、管道埋深)的关系。结果表明:仿真结果具有一定的离散性,随着模型尺寸和颗粒数量的增加,离散性会减小;管道埋深和管径均与最大附加力亦呈线性关系,当管道埋深和管径同时增加时,最大附加力呈幂函数形式增加。在此基础上,提出了基于管径和管道埋深预估最大附加力的经验公式。提出的将颗粒离散元法应用于土洞塌陷时管土相互作用的准静态过程模拟具有潜在的应用前景,其结果可为岩溶塌陷区管道支撑防护结构的设计提供理论依据。

关键词：岩溶塌陷管道工程管土相互作用颗粒离散元准静态

苦丁茶结合酚的提取优化及其对α-葡萄糖苷酶的抑制作用

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食品研究与开发 2023年第16期44卷 1-8页

作者：厉莉史硕硕王鑫耿可赞张飞俊胡婷武汉工程大学环境生态与生物工程学院湖北武汉430205

以苦丁茶为原料,根据单因素试验结果,通过响应面法优化苦丁茶结合酚的最佳提取条件,采用不同型号大孔树脂对苦丁茶结合酚进行吸附,以除去粗提物中的盐,并且研究其对淀粉消化酶α-葡萄糖苷酶的抑制作用。试验表明,苦丁茶结合酚最佳提取... 详细信息

以苦丁茶为原料,根据单因素试验结果,通过响应面法优化苦丁茶结合酚的最佳提取条件,采用不同型号大孔树脂对苦丁茶结合酚进行吸附,以除去粗提物中的盐,并且研究其对淀粉消化酶α-葡萄糖苷酶的抑制作用。试验表明,苦丁茶结合酚最佳提取条件为氢氧化钠浓度8.5 mol/L、液料比43∶1(mL/g)、提取时间4.2 h,此优化条件下,测得结合酚含量为(29.8±2.0)mg/g;7种不同类型的大孔树脂中,AB-8大孔树脂对苦丁茶结合酚的吸附率和解吸率最高;苦丁茶结合酚对α-葡萄糖苷酶的抑制作用明显,其半抑制浓度为1.90 mg/mL,抑制类型为可逆性抑制,并且是非竞争性抑制类型,抑制常数K_(i)为0.60 mg/mL。

关键词：苦丁茶结合酚提取优化 α-葡萄糖苷酶抑制作用