Natural alleles of a uridine 5'-diphospho-glucosyltransferase gene responsible for differential endosperm development between upland rice and paddy rice

作     者：Zihao Wu Xiao Zhang Guimei Chang Jun Yang Jinpeng Wan Feijun Wang Dayun Tao Jiawu Zhou Lianguang Shang Peng Xu Diqiu Yu Zihao Wu;Xiao Zhang;Guimei Chang;Jun Yang;Jinpeng Wan;Feijun Wang;Dayun Tao;Jiawu Zhou;Lianguang Shang;Peng Xu;Diqiu Yu

作者机构：School of Life SciencesUniversity of Science and Technology of ChinaHefei 230027China CAS Key Laboratory of Tropical Plant Resources and Sustainable UseXishuangbanna Tropical Botanical Gardenthe Chinese Academy of SciencesKunming 650223China Yunnan Key Laboratory for Rice Genetic ImprovementFood Crops Research InstituteYunnan Academy of Agricultural SciencesKunming 650200China The Innovative Academy of Seed DesignChinese Academy of SciencesMengla 666303China Shenzhen BranchGuangdong Laboratory of Lingnan Modern Agricultural and Rural AffairsAgricultural Genomics Institute at ShenzhenChinese Academy of Agricultural SciencesShenzhen 518000China Center of Economic BotanyCore Botanical GardensChinese Academy of SciencesMergla 666303China State Key Laboratory for Conservation and Utilization of Bio-Resources in YunnanYunnan UniversityKunming 650091China 

出 版 物：《Journal of Integrative Plant Biology》 (植物学报（英文版）)

年 卷 期：2022年第64卷第1期

页      面：135-148页

核心收录：

学科分类：09[农学] 0901[农学-作物学] 

基　　金：supported by the National Natural Science Foundation of China(Grant Nos.U1702231,31360330,31902110) The Science and Technology Projects of Yunnan Province,China(Grant No.202003AD150007) Strategic Leading Science and Technology Program of Chinese Academy of Sciences(Grant Nos.XDA24030301 and XDA24040308) Natural Science Foundation of Yunnan,China(Grant No.2018FA 023) 

主　　题：EDR1 endosperm development grain quality UDP-glucosyltransferase upland rice 

摘      要：Traditional upland rice generally exhibits insufficient grains resulting from abnormal endosperm development compared to paddy rice. However, the underlying molecular mechanism of this trait is poorly understood. Here,we cloned the uridine 5’-diphospho(UDP)-glucosyltransferase gene EDR1(Endosperm Development in Rice) responsible for differential endosperm development between upland rice and paddy rice by performing quantitative trait loci analysis and map-based cloning. EDR1 was highly expressed in developing seeds duringgrain filling. Natural variations in EDR1 significantly reduced the UDP-glucosyltransferase activity of EDR1 YZNcompared to EDR1 YD1,resulting in abnormal endosperm development in the near-isogenic line, accompanied by insufficient grains and changes in grain *** analyzing the distribution of the two alleles EDR1 YD1 and EDR1 YZNamong diverse paddy rice and upland rice varieties, we discovered that EDR1 was conserved in upland rice, but segregated in paddy rice. Further analyses of grain chalkiness in the alleles of EDR1 YD1 and EDR1 YZNvarieties indicated that rice varieties harboring EDR1 YZNand EDR1 YD1 preferentially showed high chalkiness, and low chalkiness,respectively. Taken together, these results suggest that the UDP-glucosyltransferase gene EDR1 is an important determinant controlling differential endosperm development between upland rice and paddy rice.