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Genome-wide profiling of circular RNAs,alternative splicing,and R-loops in stem-differentiating xylem of Populus trichocarpa

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Journal of Integrative Plant Biology 2021年第7期63卷 1294-1308页

作者： Xuqing Liu Yubang Gao Jiakai Liao Miao Miao Kai Chen feihu xi Wentao Wei Huihui Wang Yongsheng Wang xiXu Anireddy S.N.Reddy Lianfeng Gu Basic Forestry and Proteomics Research Center College of ForestryFujian Provincial Key Laboratory of Haixia Applied Plant Systems BiologyFujian Agriculture and Forestry UniversityFuzhou 350002China Basic Forestry and Proteomics Research Center College of Life ScienceFujian Agriculture and Forestry UniversityFuzhou 350002China Department of Biology and Program in Cell and Molecular Biology Colorado State UniversityFort CollinsColorado 80523USA

Circular RNAs(circRNAs)are a recently dis-covered type of non‐coding RNA derived from pre‐mRNAs.R‐loops consist of a DNA:RNA hybrid andthe associated single‐stranded ***-dopsis thaliana,circRNA:DNA R‐loops regulatealternative splicing(AS)ofSEPALLATA3(SEP3).However,the occurrence and functions ofcircRNAs and R‐loops inPopulus trichocarpaarelargely ***,we performed circRNA‐enriched sequencing in the stem‐differentiatingxylem(SDX)*** identified 2,742distinct circRNAs,including circ‐CESA4,circ‐IRX7,and circ‐GUX1,which are generated from genesinvolved in cellulose,and hemicellulose biosyn-thesis,*** investigate the roles ofcircRNAs in modulating alternative splicing(AS),we detected 7,836 AS events using PacBio Iso‐Seq and identified 634 circRNAs that overlappedwith 699 AS ***,using DNA:RNAhybrid immunoprecipitation followed by se-quencing(DRIP‐seq),we identified 8,932 R‐looppeaks that overlapped with 181 circRNAs and 672AS ***,several SDX‐related circRNAsoverlapped with R‐loop peaks,pointing to theirpossible roles in modulating AS in ***,overexpressing circ‐IRX7increased the levels ofR‐loop structures and decreased the frequency ofintron retention in *** provides a valuable R‐loop atlas resourceand uncovers the interplay between circRNAs andAS in SDX ***.

关键词： alternative splicing circular RNA R‐loop Populustrichocarpa stem‐differentiating xylem

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Profiling of circular RNA N^(6)-methyladenosine in moso bamboo(Phyllostachys edulis)using nanopore-based direct RNA sequencing

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Journal of Integrative Plant Biology 2020年第12期62卷 1823-1838页

作者： Yongsheng Wang Huihui Wang feihu xi Huiyuan Wang ximei Han Wentao Wei Hangxiao Zhang Qianyue Zhang Yushan Zheng Qiang Zhu Markus V.Kohnen Anireddy S.N.Reddy Lianfeng Gu Basic Forestry and Proteomics Research Center College of life scienceFujian Agriculture and Forestry UniversityFuzhou 350002China Basic Forestry and Proteomics Research Center College of ForestryFujian Agriculture and Forestry UniversityFuzhou 350002China College of Forestry Fujian Agriculture and Forestry UniversityFuzhou 350002China Department of Biology and Program in Cell and Molecular Biology Colorado State UniversityFort CollinsColoradoUSA

N6-methyladenosine(m^(6)A)is a prevalent modification in messenger RNAs and circular RNAs that play important roles in regulating various aspects of RNA ***,the occurrence of the m^(6)A modification in plant circular RNAs has not been reported.A widely used method to identify m^(6)A modifications relies on m^(6)A-specific antibodies followed by next-generation sequencing of precipitated RNAs(MeRIP-Seq).However,one limitation of MeRIP-Seq is that it does not provide the precise location of m^(6)A at single-nucleotide *** more recent sequencing techniques such as Nanopore-based direct RNA sequencing(DRS)can overcome such limitations,the technology does not allow sequencing of circular RNAs,as these molecules lack a poly(A)***,we developed a novel method to detect the precise location of m^(6)A modifications in circular RNAs using Nanopore *** first enriched our samples for circular RNAs,which we then fragmented and sequenced on the Nanopore platform with a customized *** this method,we identified 470 unique circular RNAs from DRS reads based on the back-spliced junction *** exonic circular RNAs,about 10%contained m^(6)A sites,which mainly occurred around acceptor and donor splice *** study demonstrates the utility of our antibody-independent method in identifying total and methylated circular RNAs using Nanopore *** method has the additional advantage of providing the exact location of m^(6)A sites at single-base resolution in circular RNAs or linear transcripts from non-coding RNA without poly(A)tails.

关键词： circular precise overcome

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不同生态区烤烟K326质量特征差异分析

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福建农林大学学报（自然科学版） 2016年第3期45卷 241-246页

作者：尤垂淮江丽芳席飞虎杜礼渊张涛古力林文雄张重义福建农林大学烟草研究所福建福州350002 川渝中烟工业有限责任公司四川成都610017

对福建泰宁、云南祥云、河南宜阳生态区烤烟K326主要质量特征差异进行了研究.结果表明,泰宁生态区和祥云生态区烤烟为清香型,宜阳生态区烤烟为浓香型.泰宁生态区和祥云生态区烤烟植株相对高大,而宜阳生态区烤烟植株相对较小.泰宁生态区... 详细信息

对福建泰宁、云南祥云、河南宜阳生态区烤烟K326主要质量特征差异进行了研究.结果表明,泰宁生态区和祥云生态区烤烟为清香型,宜阳生态区烤烟为浓香型.泰宁生态区和祥云生态区烤烟植株相对高大,而宜阳生态区烤烟植株相对较小.泰宁生态区和祥云生态区烤烟类胡萝卜素及其降解产物(β-大马酮、β-二氢大马酮、藏花醛、β-环柠檬醛、二氢猕猴桃内酯等)、类胡萝卜素降解产物总量,叶绿素及其降解产物新植二烯,以及烤烟还原糖和总糖等指标含量高于宜阳生态区烤烟.宜阳生态区烤烟类胡萝卜素相关降解产物(茄酮、巨豆三烯酮1、巨豆三烯酮2、巨豆三烯酮4等)、苯丙氨酸降解产物(苯甲醛、苯乙醛)及其总量,以及烤烟总碱和氯等指标含量高于泰宁生态区和祥云生态区烤烟.可见,不同生态区烤烟K326外观形态、常规化学成分及香气物质含量、香型均存在差异.

关键词：烤烟 K326 类胡萝卜素感官质量

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烟草烟碱合成代谢调控研究进展

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亚热带农业研究 2016年第1期12卷 62-67页

作者：王威威席飞虎杨少峰江丽芳王峰吉福建农林大学作物科学学院福建福州350002

烟碱是烟草最主要的生物碱,直接影响着烟叶的质量及烟叶的可用性。对烟碱合成代谢调控的研究现状进行了综述,包括论述了环境条件、栽培措施、外源激素、烘烤调制等因素对烟碱代谢的调控效果以及烟碱代谢关键基因的研究进展,并提出烟碱... 详细信息

烟碱是烟草最主要的生物碱,直接影响着烟叶的质量及烟叶的可用性。对烟碱合成代谢调控的研究现状进行了综述,包括论述了环境条件、栽培措施、外源激素、烘烤调制等因素对烟碱代谢的调控效果以及烟碱代谢关键基因的研究进展,并提出烟碱合成的根特异性遗传机理、烟碱代谢相关调控基因的分离鉴定及其同源基因的发现是后续的研究方向。

关键词：烟草烟碱合成代谢调控基因

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植物CRISPR/Cas技术研究进展及其在林业科学研究中的应用

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福建农林大学学报（自然科学版） 2021年第3期50卷 289-300页

作者：胡凯强廖家凯席飞虎高鹏飞陈凯魏文桃丁家治苗苗顾连峰福建农林大学林学院福建福州350002 福建农林大学生命科学学院福建福州350002

近年来,CRISPR/Cas系统在基因编辑领域展现出强大的定点编辑能力,在植物基础研究和分子育种等方面发挥了不可替代的作用.本文综述了CRISPR/Cas系统的发展历程,及其在植物研究应用中的遗传转化方法和鉴定方法等;同时还介绍了这种基因编... 详细信息

近年来,CRISPR/Cas系统在基因编辑领域展现出强大的定点编辑能力,在植物基础研究和分子育种等方面发挥了不可替代的作用.本文综述了CRISPR/Cas系统的发展历程,及其在植物研究应用中的遗传转化方法和鉴定方法等;同时还介绍了这种基因编辑技术在植物领域的多种研究新方向;并详细概述了CRISPR/Cas系统在林木基因功能分析鉴定等基础科研方面的应用情况;最后展望了该技术在林业科学研究中的应用前景,以期为后续相关的林木遗传改良等研究提供参考.

关键词：基因编辑林木分子育种遗传转化

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Preparation and Photocatalytic Activity of Yellow Titania

Preparation and Photocatalytic Activity of Yellow Titania

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中国材料大会2012

作者： Lin Li Shengqi xi Yangnan xing feihu Shang State Key Laboratory for Mechanical Behavior of Materials School of Materials Science and Engineering Xi’an Jiaotong University

ISBN: (纸本)9783037856062;3037856068

Titania powder was prepared by a sol-gel method and heat-treatment （HT）. The morphology and structure of the titania powder were characterized by X-ray diffraction （XRD）, differential thermal analysis, transmission electron microscopy （TEM） and X-ray photoelectron spectroscopy （XPS）. The photocatalytic activity of powder was investigated using methyl orange as target compound. Colour changing of powder was due to colour centers form. Under visible light, yellow powder showed the better photocatalytic activity than P25 powder and its visible light response was expanded. Preparation of yellow powder lowered conventional calcinations temperature obviously.

关键词： Titania Oxygen vacancy Photocatalysis Methyl orange

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Suppression Strategy of Micro-waviness error in Ultra-precision Parallel Grinding

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Nanomanufacturing and Metrology 2022年第4期5卷 423-429页

作者： Shanshan Chen Shuming Yang Chi Fai Cheung Lai Ting Ho feihu Zhang xi’an Jiaotong University 28 Xianning West RoadXi’an710049China Research Institute of xi’an Jiaotong University ZhejiangHangzhou311215China The Hong Kong Polytechnic University Hung HomKowloon999077Hong KongChina Harbin Institute of Technology Harbin150001China

Ultra-precision parallel grinding is widely used in the machining of complex optic components with high tolerance and excellent surface ***,the micro-waviness raised from the relative motion error of the grinding tool is frequently involved in the grinding process despite the fine dressing and dynamic balance work carried out,which posed a remarkable impact on the surface quality and form ***,it is essential to investigate the evolution mechanism of the micro-waviness error and determine a relevant strategy to suppress this kind of *** this paper,a model of the distribution of grinding points corresponding to the relative motion error of the grinding wheel is developed by considering the phase effect.A close relationship is found between the micro-waviness geometry and the distribution of grinding *** indicates that the phase shift is a significant parameter to determine the waviness pattern,and the uniform distribution of grinding points is beneficial to suppress the micro-waviness in parallel ***,an error-suppression strategy is proposed by adjusting the wheel speed to maintain an appropriate phase shift to suppress the micro-waviness *** work provides a new method to control the micro-waviness and reduce the effect of the waviness error on the surface generation.

关键词： Suppression strategy Micro-waviness Motion error Parallel grinding

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Lensless imaging through thin scattering layers under broadband illumination

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Photonics Research 2022年第11期10卷 2471-2487页

作者： WEI LI BINGJIAN WANG TENGFEI WU feihu XU xiAOPENG SHAO School of Optoelectronic Engineering Xidian UniversityXi’an 710071China School of Physics Xidian UniversityXi’an 710071China Laboratoire Kastler Brossel ENS–UniversitéPSLCNRSSorbonne UniversitéCollege de FranceF-75005 ParisFrance Hefei National Laboratory for Physical Sciences at Microscale and School of Physical Science University of Science and Technology of ChinaHefei 230026China

Lensless scattering imaging is a prospective approach to microscopy in which a high-resolution image of an object is reconstructed from one or more measured speckle patterns, thus providing a solution in situations where the use of imaging optics is not possible. However, current lensless scattering imaging methods are typically limited by the need for a light source with a narrowband spectrum. Here, we propose two general approaches that enable single-shot lensless scattering imaging under broadband illumination in both noninvasive [without point spread function(PSF) calibration] and invasive(with PSF calibration) modes. The first noninvasive approach is based on a numerical refinement of the broadband pattern in the cepstrum incorporated with a modified phase retrieval strategy. The latter invasive approach is correlation inspired and generalized within a computational optimization framework. Both approaches are experimentally verified using visible radiation with a full-width-at-half-maximum bandwidth as wide as 280 nm(Δλ∕λ = 44.8%) and a speckle contrast ratio as low as 0.0823. Because of its generality and ease of implementation, we expect this method to find widespread applications in ultrafast science,passive sensing, and biomedical applications.

关键词： spectrum illumination speckle

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Study on multi-objective resources matching scheduling algorithm based on network structure

Study on multi-objective resources matching scheduling algor...

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2011 International Conference on Computers, Communications, Control and Automation

作者： feihu Hu Ling Ma Le Liu Jie Gao School of Electrical Engineering Xi’an Jiaotong University School of Management Xi’an Jiaotong University

For multi-objective resources matching problem based on network structure, this paper proposes an improved micro population genetic algorithm, which can solve the emergency resources scheduling problem under the condition of multi-depot, multi-disaster places, multi-resource, multipath types, and multiple vehicle types with the goal of the shortest time and the smallest cost.

关键词： resources matching emergency management vehicle rounting improved micro population genetic algorithm

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Association of ABCB1 Gene Polymorphisms with Efficacy and Adverse Reaction to Risperidone or Paliperidone in Han Chinese Schizophrenic Patients

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Neuroscience Bulletin 2016年第6期32卷 547-549页

作者： Weifeng Mi feihu Liu Yongqiao Liu Bo Du Weidong xiao Lingzhi Li Lan Huang Tianlan Lu Jia He Le Shi Weihua Yue Hongyan Zhang Peking University Sixth Hospital Peking University Instituteof Mental Health Key Laboratory of Mental Health Ministry of Health (Peking University) National Clinical Research Center for Mental Disorders (Peking University Sixth Hospital) Beijing 100191 China The Mental Health Center of xi'an Xi'an 710061 China The Sixth People's Hospital of Hebei Province Baoding 07100 China The People's Hospital of Hubei Province Wuhan 430060China National Institute on Drug Dependence and Beijing KeyLaboratory of Drug Dependence Peking UniversityBeijing 100191 China Department of Pharmacology School of Basic Medical Sciences Peking University Health Science CenterBeijing 100191 China

Dear editor, P-glycoprotein （P-gp, also known as ATP-binding cassette transport sub-family B member 1, ABCB1） is a potent ATP-dependent efflux pump for a wide variety of drugs. Although studies of its substrates are abundant [ 1, 2], and ABCB1 is a well-conserved gene, there is increasing evi- dence that its polymorphisms affect substrate specificity [3]. A previous study reported that the synonymous single nucleotide polymorphism （SNP） C3435T （rs1045642） affects the timing of co-translational folding and insertionof P-gp into the membrane,

关键词： PANSS Association of ABCB1 Gene Polymorphisms with Efficacy and Adverse Reaction to Risperidone or Paliperidone in Han Chinese Schizophrenic Patients

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