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基于症状群、心理应激探究肺癌术后患者早期生存质量的影响因素

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沈阳医学院学报 2024年第4期26卷 382-388,408页

作者：祝志邦时静房汝敏齐志锋皮飞飞焦作市第二人民医院麻醉手术部河南焦作454000

目的:观察肺癌术后患者早期生存质量与症状群、心理应激的关系及其影响因素,为临床及时控制症状群,提高心理应激状态,改善患者术后早期生存质量奠定基础。方法:选取2021年10月至2023年10月于我院接受外科手术治疗的肺癌患者248例,采用... 详细信息

目的:观察肺癌术后患者早期生存质量与症状群、心理应激的关系及其影响因素,为临床及时控制症状群,提高心理应激状态,改善患者术后早期生存质量奠定基础。方法:选取2021年10月至2023年10月于我院接受外科手术治疗的肺癌患者248例,采用一般情况调查表及生活质量评估量表(FACT)、安德森症状评估量表、症状自评量表(SCL-90)对患者一般情况、生存质量、症状群及心理应激情况进行评估。采用Pearson法分析FACT评分与症状群、SCL-90评分的相关性,在多因素Logistic回归分析基础上采用累积比数回归模型行肺癌术后早期生存质量分析。结果:共239例纳入研究,肺癌患者的FACT-L得分为(53.66±6.85)分,SCL-90得分为(109.24±11.97)分,症状群得分为(104.03±16.83)分,其中生存质量低分组117例,中分组69例,高分组53例,分别占48.95%、28.87%、22.18%;Pearson分析显示FACT评分与SCL-90评分、症状群均呈负相关(r分别为-0.779、-0.737,P<0.05);多因素累积比数Logistic回归分析显示集束化气道管理、睡眠质量是肺癌术后患者早期生存质量的保护因素(P<0.05),营养不良、疼痛程度、手术持续时间、SCL-90评分、症状群评分是肺癌术后患者早期生存质量的危险因素(P<0.05)。结论:肺癌术后早期生存质量的影响因素来自于多个方面,临床可根据影响因素制定防控策略,以提升肺癌患者术后早期生存质量水平。

关键词：生存质量心理应激症状群肺癌影响因素

自体富血小板血浆联合封闭负压引流治疗胫前慢性难愈性创面的临床疗效观察

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生物骨科材料与临床研究 2024年第2期21卷 57-60页

作者：浦飞飞赵晓龙杨林刘伟冯晶夏平武汉市第一医院骨科湖北武汉430022 武汉市第四医院骨科湖北武汉430030

目的研究应用自体富血小板血浆(platelet-rich plasma, PRP)联合持续封闭负压引流(vacuum sealing drainage, VSD)治疗胫前慢性难愈性创面患者的临床治疗效果。方法选择2018年1月至2022年12月武汉市第一医院收治的胫前慢性难愈性创面... 详细信息

目的研究应用自体富血小板血浆(platelet-rich plasma, PRP)联合持续封闭负压引流(vacuum sealing drainage, VSD)治疗胫前慢性难愈性创面患者的临床治疗效果。方法选择2018年1月至2022年12月武汉市第一医院收治的胫前慢性难愈性创面患者40例,按随机数字表法分为PRP+VSD组和VSD组,每组20例。PRP+VSD组采用PRP联合VSD治疗,VSD组采用VSD治疗。比较两组患者的住院时间、抗菌药物使用时间、创面愈合时间、医疗费用和创面愈合情况。结果两组患者均获随访,PRP+VSD组随访时间2~12个月(平均3.72个月),VSD组随访时间3~14个月(平均4.25个月)。PRP+VSD组的住院时间、抗菌药物使用时间、创面愈合时间均低于VSD组(P0.05)。PRP+VSD组20例患者创面全部愈合,其中16例患者一次性愈合,4例患者经过2次PRP+VSD治疗后愈合。VSD组9例患者一次性愈合,其余11例患者均经多次清创和VSD治疗才使创面完全愈合。结论自体PRP联合VSD治疗胫前慢性难愈性创面可缩短创面愈合时间、患者住院时间和抗菌药物使用时间,降低二次手术率。自体PRP联合VSD是治疗胫前慢性难愈性创面的有效方法,为慢性难愈性创面的修复治疗提供了临床指导。

关键词：慢性难愈性创面胫前富血小板血浆封闭负压引流

Oxidized mitochondrial DNA sensing by STING signaling promotes the antitumor effect of an irradiated immunogenic cancer cell vaccine

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Cellular & Molecular Immunology 2021年第9期18卷 2211-2223页

作者： Chunju Fang Fei Mo Li Liu jing Du Min Luo Ke Men feifei Na Wei Wang Hanshuo Yang Xiawei Wei Laboratory of Aging Research and Cancer Drug Target State Key Laboratory of BiotherapyNational Clinical Research Center for GeriatricsWest China HospitalSichuan University610041 ChengduP.R.China Department of Oncology Guizhou Provincial People’s Hospital550002 GuiyangGuizhouP.R.China

Exposure to ionizing radiation,a physical treatment that inactivates live tumor cells,has been extensively applied to enhance the antitumor responses induced by cancer cell vaccines in both animal research and human clinical ***,the mechanisms by which irradiated cells function as immunogenic tumor vaccines and induce effective antitumor responses have not been fully ***,we demonstrate that oxidized mitochondrial DNA(mtDNA)and stimulator of interferon genes(STING)signaling play a key roles in the enhanced antitumor effect achieved with an irradiated tumor cell *** in ROS and oxidized mtDNA 8-OHG content could be induced in irradiated tumor *** mtDNA derived from irradiated tumor cells gained access to the cytosol of dendritic cells(DCs).Oxidized mtDNA,as a DAMP or adjuvant,activated the STING-TBK1-IRF3-IFN-β pathway in DCs,which subsequently cross-presented irradiated tumor cell-derived antigens to CD8^(+)T cells and elicited antitumor *** results of our study provide insight into the mechanism by which an irradiated cell vaccine mediates antitumor immunity,which may have implications for new strategies to improve the efficacy of irradiated vaccines.

关键词： Irradiated tumor cell vaccine Oxidized mitochondrial DNA STING signaling

步态训练与评估系统联合中药热奄包疗法在脑卒中偏瘫病人中的应用

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循证护理 2024年第16期10卷 2991-2995页

作者：吴小妹焦建东冯乐黄飞飞严婧东台市中医院江苏224200

目的:探究步态训练与评估系统联合中药热奄包疗法在脑卒中偏瘫病人中的应用。方法:选取我院2022年7月—2023年6月收治的86例脑卒中偏瘫病人为研究对象,根据其入院时间的先后顺序进行分组,2022年7月—12月收治的43例病人为对照组,2023年... 详细信息

目的:探究步态训练与评估系统联合中药热奄包疗法在脑卒中偏瘫病人中的应用。方法:选取我院2022年7月—2023年6月收治的86例脑卒中偏瘫病人为研究对象,根据其入院时间的先后顺序进行分组,2022年7月—12月收治的43例病人为对照组,2023年1月—6月收治的43例病人为干预组;对照组实施常规康复护理,干预组在其基础上实施步态训练与评估系统联合中药热奄包疗法,干预8周后比较两组病人的平衡功能和步态恢复情况。结果:干预8周后,干预组病人的Berg平衡量表(BBS)评分及步速、跨步长、步频高于对照组,重心移动轨迹总长度及面积、步宽低于对照组,差异均有统计学意义(P<0.05)。结论:步态训练与评估系统联合中药热奄包疗法能有效提升脑卒中偏瘫病人的平衡功能,纠正其步态异常。

关键词：脑卒中偏瘫步态训练与评估系统中药热奄包疗法平衡功能护理

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蒸汽涡街流量计跨介质标定研究

自动化与仪器仪表 2024年第9期 131-133,139页

作者：康勇李靖赵进涛周方段飞飞张弛陕西省计量科学研究院西安710100

为实现蒸汽涡街流量计的跨介质标定;在雷诺数相似准则指导下,分别采用水介质和蒸汽介质做了涡街流量计实流标定实验,得到不同介质下的流量计仪表系数;结合理论分析,总结归纳出介质可压缩性和温度两个重要参数,参考差压式流量计对介质可... 详细信息

为实现蒸汽涡街流量计的跨介质标定;在雷诺数相似准则指导下,分别采用水介质和蒸汽介质做了涡街流量计实流标定实验,得到不同介质下的流量计仪表系数;结合理论分析,总结归纳出介质可压缩性和温度两个重要参数,参考差压式流量计对介质可压缩性的修正,首次提出基于介质可压缩性和温度修正的蒸汽涡街流量计仪表系数模型;采用最小二乘回归法对涡街流量计标定实验数据进行回归分析,得到由水介质到蒸汽介质的跨介质标定仪表系数修正公式,与实验数对比验证,修正误差为0.03%~0.04%;结果表明,可以通过仪表系数修正的方法完成蒸汽涡街流量计跨介质标定。

关键词：蒸汽涡街流量计仪表系数跨介质标定可压缩性

C5orf34基因过表达慢病毒载体的构建及鉴定

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新疆医学 2024年第6期54卷 655-659页

作者：阳梦郭菲菲赵静李芳吕爱霞何丹新疆医科大学附属肿瘤医院胸外科乌鲁木齐830011

目的构建C5orf34基因的过表达慢病毒载体。方法通过聚合酶链反应(PCR)选择性扩增C5orf34基因目的片段,将目的片段连接至载体,利用基因重组技术构建带有C5orf34基因目的片段的重组载体,将重组载体转化到感受态细胞扩增并对阳性克隆的C5or... 详细信息

目的构建C5orf34基因的过表达慢病毒载体。方法通过聚合酶链反应(PCR)选择性扩增C5orf34基因目的片段,将目的片段连接至载体,利用基因重组技术构建带有C5orf34基因目的片段的重组载体,将重组载体转化到感受态细胞扩增并对阳性克隆的C5orf34过表达慢病毒载体进行测序,将正确重组的过表达慢病毒载体包装并转染色至293T细胞进行后续的慢病毒转染和滴度测定,采用实时定量PCR法检测C5orf34基因的过表达水平。结果经过测序比对显示,慢病毒载体与设计参考序列一致。重组慢病毒经包装并转染293T细胞后可观察到荧光,显示C5orf34过表达慢病毒载体滴度为1.0×10^(8)TU/mL。实时定量PCR的结果显示,在稳定转染C5orf34基因的细胞中C5orf34基因表达水平明显增高。结论成功构建C5orf34基因过表达慢病毒载体,为进一步研究C5orf34基因在肺癌发生发展过程中的机制奠定了生物学基础。

关键词： C5orf34 慢病毒表达载体非小细胞肺癌

Bacteria-mediated tumor-targeted delivery of tumstatin(54-132)significantly suppresses tumor growth in mouse model by inhibiting angiogenesis and promoting apoptosis

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Frontiers of Medicine 2022年第6期16卷 873-882页

作者： feifei Bao Mengjie Liu Wenhua Gai Yuwei Hua jing Li Chao Han Ziyu Zai Jiahuang Li Zichun Hua The State Key Laboratory of Pharmaceutical Biotechnology School of Life SciencesNanjing UniversityNanjing210023China Changzhou High-Tech Research Institute of Nanjing University and Jiangsu Target Pharma Laboratories Inc. Changzhou213164China

Tumor growth is an angiogenesis-dependent process and accompanied by the formation of hypoxic *** is a tumor-specific angiogenesis inhibitor that suppresses the proliferation and induces the apoptosis of tumorous vascular endothelial ***20009,an attenuated Salmonella typhimurium strain,preferentially accumulates in the hypoxic areas of solid *** this study,a novel Salmonella-mediated targeted expression system of tumstatin(VNP-Tum5)was developed under the control of the hypoxia-induced J23100 promoter to obtain anti-tumor efficacy in *** with VNP-Tum5 effectively suppressed tumor growth and prolonged survival in the mouse model of B16F10 ***-Tum5 exhibited a higher efficacy in inhibiting the proliferation and inducing the necrosis and apoptosis of B16F10 cells in vitro and in vivo compared with VNP(control).VNP-Tum5 significantly inhibited the proliferation and migration of mouse umbilical vascular endothelial cells to impede ***-Tum5 downregulated the expression of anti-vascular endothelial growth factor A,platelet endothelial cell adhesion molecule-1,phosphorylated phosphoinositide 3 kinase,and phosphorylated protein kinase B and upregulated the expression of cleaved-caspase 3 in tumor *** study is the first to use tumstatin-transformed VNP20009 as a tumor-targeted system for treatment of melanoma by combining anti-tumor and anti-angiogenic effects.

关键词： Salmonella VNP20009 tumstatin B16F10 melanoma apoptosis angiogenesis

Hypothalamic-Pituitary-Gonadal(HPG)Axis and Transcriptional Regulatory Elements Regulate piwil2 Gene Expression During Gametogenesis and Gonadal Development in Japanese Flounder(Paralichthys olivaceus)

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Journal of Ocean University of China 2020年第6期19卷 1378-1388页

作者： NI feifei YU Haiyang QU Jiangbo MENG Lihui LIU Xiumei YAN Weijie CHANG jing ZHANG Quanqi WANG Xubo YU Haiyang Key Laboratory of Marine Genetics and Breeding Ministry of EducationOcean University of ChinaQingdao 266003China College of Life Sciences Yantai UniversityYantai 264005China

The P-element induced wimpy testis(Piwi)proteins,which are associated with PIWI-interacting RNAs(piRNAs),play important roles in meiosis,germ cell division,and germline *** this study,we identified and characterized the Paralichthys olivaceus piwil2 gene,a constituent factor of the piRNA pathways involved in the biogenesis of reproductive *** biological analysis indicated that piwil2,which contains PAZ and PIWI domains,was highly conserved between teleosts and *** piwil2 distribution profile in different tissues confirmed a sexually dimorphic expression pattern,with a higher expression level in *** situ hybridization demonstrated that piwil2 was expressed in the oogonia and oocytes of the ovaries as well as in the Sertoli cells and spermatocytes of the *** piwil2 showed a maternally inherited expression pattern during embryonic development,and was highly expressed during the early embryonic *** luciferase reporters were constructed to determine the transcriptional regulatory mechanisms of *** piwil2 core promoter region was located at−360 bp to−60 ***,some representative sex hormones,including human chorionic gonadotropin,17α-methyltestosterone,and estradiol-17βhad distinct regulatory effects on *** a summery,these results indicate that piwil2,regulated by sex hormones and transcriptional elements,has vital functions in the reproductive cycle and gonadal development.

关键词： piwil2 gametogenesis and gonadal development HPG axis transcriptional regulatory elements Paralichthys olivaceus

Tumor necrosis factor a accelerates Hep-2 cells proliferation by suppressing TRPP2 expression

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Science China(Life Sciences) 2017年第11期60卷 1251-1259页

作者： jing Wu Jizheng Guo Yunyun Yang feifei Jiang Shuo Chen Kaile Wu Bing Shen Yehai Liu Juan Du Department of Otorhinolaryngology Head and Neck Surgerythe First Affiliated Hospital of Anhui Medical UniversityHefei 230022China School of Basic Medical Sciences Anhui Medical UniversityHefei 230022China Department of Pediatrics The People's Hospital of BozhouHefei 236800China

TRPP2, a Ca2＋-permeable non-selective cation channel, has been shown to negatively regulate cell cycle, but the mechanism underlying this regulation is unknown. Tumor necrosis factor a （TNF-a） is a proinflammatory cytokine extensively involved in immune system regulation, cell proliferation and cell survival. However, the effects and mechanisms for the role of TNF-a in laryngeal cancer remain unclear. Here, we demonstrated using western blot analyses and intracellular Ca〉 concentration measurements that TNF-a treatment suppressed both TRPP2 expression and ATP-induced Ca2＋ release in a laryngeal cancer cell line （Hep-2）. Knockdown of TRPP2 by a specific siRNA significantly decreased ATP-induced Ca2＋ release and abolished the effect of TNF-a on the ATP-induced Ca2＋ release. TNF-a treatment also enhanced Hep-2 cell proliferation and growth, as determined using cell counting and flow cytometry cell cycle assays. Moreover, TNF-a treatment down-regulated phosphorylated protein kinase R-like endoplasmic reticulum kinase （p-PERK） and phosphorylated eukaryotic translation initiation factor （p-elF2c0 expression levels, without affecting PERK and elF2ct expression levels in Hep-2 cells. We concluded that suppressing TRPP2 expression and TRPP2-mediated Ca2＋ signaling may be one mechanism underlying TNF〈t-enhanced Hep-2 cell proliferation. These results offer new insights into the mechanisms of TNF-a-mediated laryngeal cancer cell proliferation, and provide evidences showing a potential role of TNF-a in the development of laryngeal cancer.

关键词： tumor necrosis factor a TRPP2 human laryngeal squamous cell carcinoma proliferation protein kinase R-likeendoplasmic reticulum kinase eukaryotic translation initiation factor