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RAD-seq data reveals robust phylogeny and morphological evolutionary history of Rhododendron

Horticultural Plant Journal 2024年第3期10卷 866-878页

作者： Yuanting Shen Gang yao Yunfei Li Xiaoling Tian Shiming Li Nian Wang Chengjun Zhang fei Wang Yongpeng Ma Yunnan Key Laboratory for Integrative Conservation of Plant Species with Extremely Small Populations Kunming Institute of BotanyChinese Academy of SciencesKunmingYunnan 650201China Key Laboratory for Plant Diversity and Biogeography of East Asia Kunming Institute of BotanyChinese Academy of SciencesKunmingYunnan 650201China University of Chinese Academy of Sciences Beijing 100049China Beijing Genomics of Institute ShenzhenGuangdong 518120China Guiyang Institute of Humanities and Technology GuiyangGuizhou 550025China State Forestry and Grassland Administration Key Laboratory of Silviculture in Downstream Areas of the Yellow River College of ForestryShandong Agricultural UniversityTai'anShandong 271018China The Southwest China of Germplasm Bank of Wild Species Kunming Institute of BotanyChinese Academy of SciencesKunmingYunnan 650201China West China Subalpine Botanical Garden Institute of BotanyChinese Academy of SciencesChengduSichuan 611834China

Rhododendron is famous for its high ornamental ***,the genus is taxonomically difficult and the relationships within Rhododendron remain *** addition,the origin of key morphological characters with high horticulture value need to be *** problems largely hinder utilization of germplasm *** studies attempted to disentangle the phylogeny of Rhododendron,but only used a few genomic markers and lacked large-scale sampling,resulting in low clade support and contradictory phylogenetic ***,we used restriction-site associated DNA sequencing(RAD-seq)data and morphological traits for 144 species of Rhododendron,representing all subgenera and most sections and subsections of this species-rich genus,to decipher its intricate evolutionary history and reconstruct ancestral *** results revealed high resolutions at subgenera and section levels of Rhododendron based on RAD-seq *** optimal phylogenetic tree and split tree recovered five lineages among ***(cladeⅠ)formed the basal *** and Azaleastrum formed cladeⅡand had sister ***Ⅲincluded all scaly rhododendron ***(cladeⅣ)formed a sister group to ***(cladeⅤ).The results of ancestral state reconstruction showed that Rhododendron ancestor was a deciduous woody plant with terminal inflorescence,ten stamens,leaf blade without scales and broadly funnelform corolla with pink or purple *** study shows significant distinguishability to resolve the evolutionary history of Rhododendron based on high clade support of phylogenetic tree constructed by RAD-seq *** also provides an example to resolve discordant signals in phylogenetic trees and demonstrates the application feasibility of RAD-seq with large amounts of missing data in deciphering intricate evolutionary ***,the reconstructed ancestral state of six important characters provides insights into the inn

关键词： Rhododendron RAD-seq Missing data Quartet sampling(QS) Ancestral state reconstruction

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Effect of K2TiO(C2O4)2 Addition in Electrolyte on the Microstructure and Tribological Behavior of Micro-Arc Oxidation Coatings on Aluminum Alloy

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Acta Metallurgica Sinica(English Letters) 2017年第11期30卷 1109-1118页

作者： Jian-Li Wang Wei Yang Da-Peng Xu Xiao-fei yao School of Materials Science and Chemical Engineering Xi'an Technological University Xi'an 710021 China

Micro-arc oxidation （MAO） coatings with different concentrations of K2TiO（C2O4）2 in the sodium silicate base electrolyte were prepared on 6061 aluminum alloy with the aim of promoting a better understanding of the formation mechanisms and tribological behaviors of the coatings. Scanning electron microscopy （SEM） assisted with energy-dis- persive X-ray spectroscopy （EDS）, X-ray photoelectron spectroscopy （XPS） and friction test were employed to charac- terize the MAO processes and microstructure of the resultant coatings. Results showed that the composition and microstructure of the coatings were significantly affected by the addition of KETiO（CaO4）2. A sealing microstructure of MAO coating was obtained with the addition of K2TiO（C2O4）2. Ti element from K2TiO（C2O4）2 was only absorbed into the defects of micropores under surface energy in the early stage, while in the later stage, Ti element was predominant in the micropores and distributed on the coatings under plasma discharge to form TiO2. It was demonstrated that Ti and Si elements from the electrolyte could interact with each other during the MAO process and the interaction mechanism was systematically analyzed. Wear resistance of the MAO coatings with K2TiO（C2O4）2 addition was significantly improved compared with that of the MAO coatings without K2TiO（C2O4）2 addition.

关键词： Aluminum alloy Micro-arc oxidation K2TiO(C2O4)2 Coating formation mechanism Tribological behavior

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Effect of navigation endoscopy combined with threedimensional printing technology in the treatment of orbital blowout fractures

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International Journal of Ophthalmology(English edition) 2024年第3期17卷 570-576页

作者： Jin-Hai Yu yao-Hua Wang Qi-Hua Xu Chao Xiong An-An Wang Hong-fei Liao School of Optometry Jiangxi Medical CollegeNanchang UniversityNanchang 330006Jiangxi ProvinceChina The Affiliated Eye Hospital Jiangxi Medical CollegeNanchang UniversityNanchang 330006Jiangxi ProvinceChina Jiangxi Clinical Research Center for Ophthalmic Disease Nanchang 330006Jiangxi ProvinceChina Jiangxi Research Institute of Ophthalmology and Visual Science Nanchang 330006Jiangxi ProvinceChina Jiangxi Provincial Key Laboratory for Ophthalmology Nanchang 330006Jiangxi ProvinceChina

●AIM:To explore the combined application of surgical navigation nasal endoscopy(NNE)and three-dimensional printing technology(3DPT)for the adjunctive treatment of orbital blowout fractures(OBF).●METHODS:Retrospective analysis was conducted on the data of patients with OBF who underwent surgical treatment at the Affiliated Eye Hospital of Nanchang University between July 2012 and November *** control group consisted of patients who received traditional surgical treatment(n=43),while the new surgical group(n=52)consisted of patients who received NNE with *** difference in therapeutic effects between the two groups was evaluated by comparing the duration of the operation,best corrected visual acuity(BCVA),enophthalmos difference,recovery rate of eye movement disorder,recovery rate of diplopia,and incidence of postoperative complications.●RESULTS:The study included 95 cases(95 eyes),with 63 men and 32 *** patients’age ranged from 5 to 67y(35.21±15.75y).The new surgical group and the control group exhibited no statistically significant differences in the duration of the operation,BCVA and enophthalmos *** recovery rates of diplopia in the new surgical group were significantly higher than those in the control group at 1mo[OR=0.03,95%CI(0.01–0.15),P<0.0000]and 3mo[OR=0.11,95%CI(0.03–0.36),P<0.0000]***,the recovery rates of eye movement disorders at 1 and 3mo after surgery were OR=0.08,95%CI(0.03–0.24),P<0.0000;and OR=0.01,95%CI(0.00–0.18),P<*** incidence of postoperative complications was lower in the new surgical group compared to the control group[OR=4.86,95%CI(0.95–24.78),P<0.05].●CONCLUSION:The combination of NNE and 3DPT can shorten the recovery time of diplopia and eye movement disorder in patients with OBF.

关键词： orbital blowout fracture three-dimensional printing endoscopy surgical navigation

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Frequency and size change of ice–snow avalanches in the central Himalaya:A case from the Annapurna II glacier

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Advances in Climate Change Research 2024年第3期15卷 464-475页

作者： yao LI Yi-fei CUI Jian-Sheng HAO Zheng-Tao ZHANG Hao WANG Jian GUO Shuo-Fan WANG State Key Laboratory of Hydroscience and Engineering Department of Hydraulic EngineeringTsinghua UniversityBeijing 100084China Key Laboratory of Hydrosphere Sciences of the Ministry of Water Resources Beijing 100084China Key Laboratory of Land Surface Pattern and Simulation Institute of Geographic Sciences and Natural Resources ResearchChinese Academy of SciencesBeijing 100101China School of National Safety and Emergency Management Beijing Normal UniversityBeijing 100875China Key Laboratory of Mountain Hazards and Earth Surface Process Institute of Mountain Hazards and EnvironmentChinese Academy of SciencesChengdu 610041China

Glaciers have retreated and shrunk in High Mountain Asia since the mid-20th century because of global warming,leading to glacier instability and hazardous iceesnow ***,the complex relationship between iceesnow avalanches and factors such as climate and potential triggers are difficult to understand because of the lack of observational ***,we addressed iceesnow avalanches on the Annapurna II glacier in Nepal,Central *** constructed an iceesnow avalanche history using long-term multi-source remote sensing images(1988-2021)and mapped the velocity fields of glaciers using cross-correlation analysis on SAR and optical ***,we investigated the impact of climate change and earthquakes on the frequency and size of iceesnow *** results demonstrate that the frequency of iceesnow avalanches has increased from 10 in 1988 to 27 in 2020,but the average area of iceesnow avalanche deposits has decreased by approximately 70%,from 3.4×10^(5) m^(2) in 1988 to 1.2×10^(5) m^(2) in *** evolutionary characteristic of ice avalanches is linked to the impact of glacier retreat(reduction in ice material supply)and increased activity under climate *** glacier movement velocity controls the size of iceesnow avalanches and can be set as an indicator for iceesnow avalanche *** the Annapurna Ⅱ glacier,an iceesnow avalanche occurred when the glacier velocities were greater than 1.5 m d^(-1).These results offer insights into iceesnow avalanche risk assessment and prediction in high-mountain areas,particularly in regions characterised by dense glacier distribution.

关键词： Iceesnow avalanche Glacier instability Climate change Central Himalaya Remote sensing

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类风湿性关节炎相关肺间质病变的HRCT表现及危险因素研究

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川北医学院学报 2024年第4期39卷 452-456页

作者：郭丹丹李林贾霜冯钰玲王菲瑶杨帆唐鸿杰李睿南充市中医医院放射科四川南充637000 川北医学院附属医院放射科四川南充637000

目的:探讨类风湿关节炎(RA)相关间质性肺疾病(ILD)的胸部高分辨率CT(HRCT)表现,并探讨发生RA-ILD的临床及实验室危险因素。方法:根据是否合并ILD将接受胸部HRCT检查的471例连续性RA患者分为RA-ILD(n=273)组和非RA-ILD组(n=198)。分析RA-... 详细信息

目的:探讨类风湿关节炎(RA)相关间质性肺疾病(ILD)的胸部高分辨率CT(HRCT)表现,并探讨发生RA-ILD的临床及实验室危险因素。方法:根据是否合并ILD将接受胸部HRCT检查的471例连续性RA患者分为RA-ILD(n=273)组和非RA-ILD组(n=198)。分析RA-ILD患者的HRCT表现及两组患者临床资料及实验室指标的差异,并探讨RA患者发生ILD及RA-ILD患者进展为普通间质性肺炎(UIP)的危险因素。结果:RA-ILD组患者年龄更大,男性比例更高(P<0.05),且高血压、糖尿病的发生率更高(P<0.05)。RA-ILD组患者血沉、C反应蛋白、类风湿因子IgM、IgA水平均高于非ILD组(P<0.05)。RA-ILD患者的HRCT以小叶间隔增厚最为常见,其次为胸膜下线、网格影、磨玻璃影及蜂窝影,且蜂窝影多见于男性患者。多因素二分类逻辑回归结果发现年龄增大(OR=1.12,95%CI:1.09~1.15,P<0.001)、男性患者(OR=1.74,95%CI:1.04~2.93,P=0.036)及C反应蛋白增高(OR=1.01,95%CI:1.00~1.01,P=0.022)是发生RA-ILD的独立危险因素。RA-ILD患者中,各临床因素及实验室指标与发生UIP型间质性肺疾病均未见明显相关性。结论:年龄增大、男性及C反应蛋白增高可能是RA患者发生ILD的危险因素,且男性患者发生UIP概率更高。

关键词：类风湿关节炎间质性肺疾病高分辨率CT 危险因素

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寒温并用代表方乌梅丸治疗恶性肿瘤的研究进展

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医学综述 2024年第4期30卷 433-438页

作者：邢烙涛陈思杨甘秦汉甘雪军董芷辛许梅姚菲方芳石玮广西中医药大学研究生院南宁530200 广西中医药大学第一附属医院肿瘤科二病区南宁530023

乌梅丸是《伤寒论》厥阴病寒热错杂证主方,随着中医药理论和临床实践的不断发展,应用范围逐渐扩大,在肺癌、胃癌、肠癌、胰腺癌、乳腺癌等诸多癌种中均有报道。临床研究中运用乌梅丸协同放化疗、靶向免疫治疗等可有效缓解治疗相关不良反... 详细信息

乌梅丸是《伤寒论》厥阴病寒热错杂证主方,随着中医药理论和临床实践的不断发展,应用范围逐渐扩大,在肺癌、胃癌、肠癌、胰腺癌、乳腺癌等诸多癌种中均有报道。临床研究中运用乌梅丸协同放化疗、靶向免疫治疗等可有效缓解治疗相关不良反应,提高患者生存质量。现代实验研究发现,乌梅丸具有调控相关基因、蛋白以抑制肿瘤细胞增殖、迁移和氧化应激等多种通路及机制的作用。探索乌梅丸调节恶性肿瘤寒热错杂微环境的作用,有望为中医药防治恶性肿瘤的研究提供新方向。

关键词：恶性肿瘤乌梅丸寒温并用法

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Proposing a machine learning approach to analyze and predict basic high-temperature properties of iron ore fines and its factors

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Journal of Iron and Steel Research(International) 2024年第5期31卷 1082-1094页

作者： Qing-ke Sun yao-zu Wang Jian-liang Zhang Zheng-jian Liu Le-le Niu Chang-dong Shan Yun-fei Ma Institute of Artificial Intelligence University of Science and Technology BeijingBeijing100083China School of Intelligence Science and Technology University of Science and Technology BeijingBeijing100083China School of Metallurgical and Ecological Engineering University of Science and Technology BeijingBeijing100083China

The basic high-temperature properties of iron ore play a crucial role in optimizing sintering and ore blending,but the testing process for these properties is complex and has significant lag time,which cannot meet the actual needs of ore blending.A prediction model for the basic high-temperature properties of iron ore fines was thus proposed based on a combination of machine learning algorithms and genetic ***,the prediction accuracy of different machine learning models for the basic high-temperature properties of iron ore fines was ***,a random forest model optimized by genetic algorithms was built,further improving the prediction accuracy of the *** test results show that the random forest model optimized by genetic algorithms has the highest prediction accuracy for the lowest assimilation temperature and liquid phase fluidity of iron ore,with a determination coefficient of 0.903 for the lowest assimilation temperature and 0.927 for the liquid phase fluidity after *** trained model meets the fluctuation requirements of on-site testing and has been successfully applied to actual production on site.

关键词： Iron ore Basic high-temperature property Machine learning Random forest Genetic algorithm

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A review of reservoir damage during hydraulic fracturing of deep and ultra-deep reservoirs

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Petroleum Science 2024年第1期21卷 384-409页

作者： Kun Zhang Xiong-fei Liu Dao-Bing Wang Bo Zheng Tun-Hao Chen Qing Wang Hao Bai Er-Dong yao Fu-Jian Zhou National Key Laboratory of Petroleum Resources and Engineering China University of Petroleum-BeijingBeijing102249China School of Mechanical Engineering Beijing Institute of Petrochemical TechnologyBeijing102617China Tuha Downhole Operation Company CNPC Xibu Drilling Engineering Company LimitedShanshan838200XinjiangChina

Deep and ultra-deep reservoirs have gradually become the primary focus of hydrocarbon exploration as a result of a series of significant discoveries in deep hydrocarbon exploration *** reservoirs present unique challenges due to their deep burial depth(4500-8882 m),low matrix permeability,complex crustal stress conditions,high temperature and pressure(HTHP,150-200℃,105-155 MPa),coupled with high salinity of formation ***,the costs associated with their exploitation and development are exceptionally *** deep and ultra-deep reservoirs,hydraulic fracturing is commonly used to achieve high and stable *** hydraulic fracturing,a substantial volume of fluid is injected into the ***,statistical analysis reveals that the flowback rate is typically less than 30%,leaving the majority of the fluid trapped within the ***,hydraulic fracturing in deep reservoirs not only enhances the reservoir permeability by creating artificial fractures but also damages reservoirs due to the fracturing fluids *** challenging“three-high”environment of a deep reservoir,characterized by high temperature,high pressure,and high salinity,exacerbates conventional forms of damage,including water sensitivity,retention of fracturing fluids,rock creep,and proppant *** addition,specific damage mechanisms come into play,such as fracturing fluid decomposition at elevated temperatures and proppant diagenetic reactions at HTHP ***,the foremost concern in deep oil and gas development lies in effectively assessing the damage inflicted on these reservoirs by hydraulic fracturing,comprehending the underlying mechanisms,and selecting appropriate ***'s noteworthy that the majority of existing studies on reservoir damage primarily focus on conventional reservoirs,with limited attention given to deep reservoirs and a lack of systematic *** light of this,our approach entails initially summarizin

关键词： Artificial fracture Deep and ultra-deep reservoir Fracture conductivity Fracturing fluid Hydraulic fracturing Reservoir damage

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Machine learning and bioinformatics to identify biomarkers in response to Burkholderia pseudomallei infection in mice

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BIOCELL 2024年第4期48卷 613-621页

作者： yao FANG fei XIA feifei TIAN L EI QU FANG YANG JUAN FANG ZHENHONG HU HAICHAO LIU Department of Respiratory and Critical Care Medicine General Hospital of Center Theater of PLAWuhan430070China School of Medicine Wuhan University of Science and TechnologyWuhan430065China School of Life Science and Engineering Southwest Jiaotong UniversityChengdu611756China

Objective:In the realm of Class I pathogens,Burkholderia pseudomallei(BP)stands out for its propensity to induce severe *** the intricate interactions between BP and host cells is imperative for comprehending the dynamics of BP infection and discerning biomarkers indicative of the host cell response ***:mRNA extraction from BP-infected mouse macrophages constituted the initial step of our *** gene expression arrays,the extracted RNA underwent conversion into digital *** percentile shift method facilitated data processing,with the identiﬁcation of genes manifesting signiﬁcant differences accomplished through the application of the ***,a comprehensive analysis involving Gene Ontology enrichment and Kyoto Encyclopedia of Genes and Genomes pathway was conducted on the differentially expressed genes(DEGs).Leveraging the ESTIMATE algorithm,gene signatures were utilized to compute risk scores for gene expression *** vector machine analysis and gene enrichment scores were instrumental in establishing correlations between biomarkers and macrophages,followed by an evaluation of the predictive power of the identiﬁed ***:The functional and pathway associations of the DEGs predominantly centered around G protein-coupled receptors.A noteworthy positive correlation emerged between the blue module,consisting of 416 genes,and the ***4,identiﬁed through support vector machine analysis among intersecting genes,indicated a robust interaction with macrophages,suggesting its potential as a robust ***4 exhibited commendable predictive efﬁcacy,with BP infection inducing its expression in both macrophages and mouse lung *** blotting in macrophages conﬁrmed a signiﬁcant upregulation of FZD4 expression from 0.5 to 24 h *** mouse lung tissue,FZD4 manifested higher expression in the cytoplasm of pulmonary epithelial cells in BP-infected lungs than in the

关键词： Burkholderia pseudomallei Microarray assay Machine learning Bioinformatics FZD4

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基于RoBERTa-Effg-Adv的实体关系联合抽取方法

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计算机技术与发展 2024年第3期34卷 147-154页

作者：姚飞杨刘晓静青海大学计算机技术与应用系青海西宁810016

实体关系抽取是构建知识图谱的关键步骤,其目的是抽取文本中的关系三元组。针对现有中文实体关系联合抽取模型无法有效抽取重叠关系三元组及提取性能不足的问题,该文提出了RoBERTa-Effg-Adv的实体关系联合抽取模型,其编码端采用RoBERTa-... 详细信息

实体关系抽取是构建知识图谱的关键步骤,其目的是抽取文本中的关系三元组。针对现有中文实体关系联合抽取模型无法有效抽取重叠关系三元组及提取性能不足的问题,该文提出了RoBERTa-Effg-Adv的实体关系联合抽取模型,其编码端采用RoBERTa-wwm-ext预训练模型对输入数据进行编码,并采用Efficient GlobalPointer模型来处理嵌套和非嵌套命名实体识别,将实体关系三元组拆分成五元组进行实体关系联合抽取。再结合对抗训练,提升模型的鲁棒性。为了获得机器可读的语料库,对相关文本书籍进行扫描,并进行光学字符识别,再通过人工标注数据的方式,形成该研究所需要的关系抽取数据集REDQTTM,该数据集包含18种实体类型和11种关系类型。实验结果验证了该方法在瞿昙寺壁画领域的中文实体关系联合抽取任务的有效性,在REDQTTM测试集上的精确率达到了94.0%,召回率达到了90.7%,F1值达到了92.3%,相比GPLinker模型,在精确率、召回率和F1值上分别提高了2.4百分点、0.9百分点、1.6百分点。

关键词： RoBERTa-wwm-ext 对抗训练关系抽取 Efficient GlobalPointer 中文实体

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