RAD-seq data reveals robust phylogeny and morphological evolutionary history of Rhododendron

作     者：Yuanting Shen Gang Yao Yunfei Li Xiaoling Tian Shiming Li Nian Wang Chengjun Zhang Fei Wang Yongpeng Ma Yuanting Shen;Gang Yao;Yunfei Li;Xiaoling Tian;Shiming Li;Nian Wang;Chengjun Zhang;Fei Wang;Yongpeng Ma

作者机构：Yunnan Key Laboratory for Integrative Conservation of Plant Species with Extremely Small PopulationsKunming Institute of BotanyChinese Academy of SciencesKunmingYunnan 650201China Key Laboratory for Plant Diversity and Biogeography of East AsiaKunming Institute of BotanyChinese Academy of SciencesKunmingYunnan 650201China University of Chinese Academy of SciencesBeijing 100049China Beijing Genomics of InstituteShenzhenGuangdong 518120China Guiyang Institute of Humanities and TechnologyGuiyangGuizhou 550025China State Forestry and Grassland Administration Key Laboratory of Silviculture in Downstream Areas of the Yellow RiverCollege of ForestryShandong Agricultural UniversityTai'anShandong 271018China The Southwest China of Germplasm Bank of Wild SpeciesKunming Institute of BotanyChinese Academy of SciencesKunmingYunnan 650201China West China Subalpine Botanical GardenInstitute of BotanyChinese Academy of SciencesChengduSichuan 611834China 

出 版 物：《Horticultural Plant Journal》 (园艺学报（英文版）)

年 卷 期：2024年第10卷第3期

页      面：866-878页

核心收录：

学科分类：090706[农学-园林植物与观赏园艺] 0907[农学-林学] 09[农学] 

基　　金：supported by Ten Thousand Talent Program of Yunnan Province(Grant No.YNWR-QNBJ-2018-174) the Key Basic Research Program of Yunnan Province,China(Grant No.202101BC070003) National Natural Science Foundation of China(Grant No.31901237) Conservation Program for Plant Species with Extremely Small Populations in Yunnan Province(Grant No.2022SJ07X-03) Key Technologies Research for the Germplasmof Important Woody Flowers in Yunnan Province(Grant No.202302AE090018) Natural Science Foundation of Guizhou Province(Grant No.Qiankehejichu-ZK2021yiban 089&Qiankehejichu-ZK2023yiban 035) 

主　　题：Rhododendron RAD-seq Missing data Quartet sampling(QS) Ancestral state reconstruction 

摘      要：Rhododendron is famous for its high ornamental ***,the genus is taxonomically difficult and the relationships within Rhododendron remain *** addition,the origin of key morphological characters with high horticulture value need to be *** problems largely hinder utilization of germplasm *** studies attempted to disentangle the phylogeny of Rhododendron,but only used a few genomic markers and lacked large-scale sampling,resulting in low clade support and contradictory phylogenetic ***,we used restriction-site associated DNA sequencing(RAD-seq)data and morphological traits for 144 species of Rhododendron,representing all subgenera and most sections and subsections of this species-rich genus,to decipher its intricate evolutionary history and reconstruct ancestral *** results revealed high resolutions at subgenera and section levels of Rhododendron based on RAD-seq *** optimal phylogenetic tree and split tree recovered five lineages among ***(cladeⅠ)formed the basal *** and Azaleastrum formed cladeⅡand had sister ***Ⅲincluded all scaly rhododendron ***(cladeⅣ)formed a sister group to ***(cladeⅤ).The results of ancestral state reconstruction showed that Rhododendron ancestor was a deciduous woody plant with terminal inflorescence,ten stamens,leaf blade without scales and broadly funnelform corolla with pink or purple *** study shows significant distinguishability to resolve the evolutionary history of Rhododendron based on high clade support of phylogenetic tree constructed by RAD-seq *** also provides an example to resolve discordant signals in phylogenetic trees and demonstrates the application feasibility of RAD-seq with large amounts of missing data in deciphering intricate evolutionary ***,the reconstructed ancestral state of six important characters provides insights into the inn