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White spot syndrome virus inactivation study by using gamma irradiation

Chinese Journal of Oceanology and Limnology 2014年第5期32卷 1024-1028页

作者： Marzieh HEIDAREH farahnaz motamedi sedeh Mehdi SOLTANI Saeed RAJABIFAR Mohammad AFSHARNASAB Aghil DASHTIANNASAB Nuclear Science and Technology Research Institute Veterinary Faculty University of Tehran Iran Fisheries Research Institute

The present study was conducted to investigate the effect of gamma irradiation on white spot syndrome virus （WS SV）. White spot syndrome virus is a pathogen of major economic importance in cultured penaeid shrimp industries. White spot disease can cause mortalities reaching 100% within 3-10 days of gross signs appearing. During the period of culture, immunostimulant agents and vaccines may provide potential methods to protect shrimps from opportunistic and pathogenic microrganisms. In this study, firstly, WSSV was isolated from infected shrimp and then multiplied in crayfish. WSSV was purified from the infected crayfish haemolymph by sucrose gradient and confirmed by transmission electron microscopy. In vivo virus titration was performed in shrimp, Penaeus semisulcatus. The LD50 of live virus stock was calculated 1054/mL. Shrimp post-larvae （1-2 g） were treated with gamma-irradiated （different doses） WSSV （10^o to 10^-4 dilutions） for a period of 10 days. The dose/survival curve for irradiated and un-irradiated WSSV was drawn; the optimum dose range for inactivation of WSSV and unaltered antigenicity was obtained 14- 15 kGy. This preliminary information suggests that shrimp appear to benefit from treatment with gamma- irradiated WSSV especially at 14-15 KGy.

关键词： white spot syndrome virus (WSSV) gamma irradiation titration LD50

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Comparison of Immune Responses against FMD by a DNA Vaccine Encoding the FMDV/O/IRN/2007 VP1 Gene and the Conventional Inactivated Vaccine in an Animal Model

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Virologica Sinica 2012年第5期27卷 286-291页

作者： farahnaz motamedi sedeh Hoorieh Soleimanjahi AmirReza Jalilian Homayoon Mahravani Agricultural Medical and Industrial Research School Virology Department Faculty of Medical ScienceTarbiat Modares University RaziVaccine and Serum Research Institute

Foot-and-mouth disease virus （FMDV） is highly contagious and responsible for huge outbreaks among cloven hoofed animals. The aim of the present study is to evaluate a plasmid DNA immunization system that expresses the FMDV/OflRN/2007 VP1 gene and compare it with the conventional inactivated vaccine in an animal model. The VP1 gene was sub-cloned into the unique Kpn I and BamH I cloning sites of the peDNA3.1＋ and pEGFP-N1 vectors to construct the VPI gene cassettes. The transfected BHKT7 cells with sub-cloned pEGFP-N1-VP1 vector expressed GFP-VP1 fusion protein and displayed more green fluorescence spots than the transfected BHKT7 cells with pEGFP-N1 vector, which solely expressed the GFP protein. Six mice groups were respectively immunized by the sub-cloned pcDNA3.1＋-VP1 gene cassette as the DNA vaccine, DNA vaccine and PCMV-SPORT-GMCSF vector （as molecular adjuvant） together, conventional vaccine, PBS （as negative control）, pcDNA3.1＋ vector （as control group） and PCMV-SPORT vector that contained the GMCSF gene （as control group）. Significant neutralizing antibody responses were induced in the mice which were immunized using plasmid vectors expressing the VP1 and GMCSF genes together, the DNA vaccine alone and the conventional inactivated vaccine （P〈0.05）. Co-administration of DNA vaccine and GMCSF gene improved neutralizing antibody response in comparison with administration of the DNA vaccine alone, but this response was the most for the conventional vaccine group. However, induction of humeral immunity response in the conventional vaccine group was more protective than for the DNA vaccine, but T-cell proliferation and IFN-？ concentration were the most in DNA vaccine with the GMCSF gene. Therefore the group that was vaccinated by DNA vaccine with the GMCSF gene, showed protective neutralizing antibody response and the most Thl cellular immunity.

关键词： DNA vaccine Foot-and-mouth disease virus Immune Response VP 1 gene

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Synthesis and <i>in vitro</i>antibacterial evaluation of some thiosemicarbazides and thiosemicarbazones

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Journal of Biomedical Science and Engineering 2012年第2期5卷 39-42页

作者： Morasa Sheikhy Amir R. Jalilian Aytak Novinrooz farahnaz motamedi-sedeh Agricultural Medical and Industrial Research School Nuclear Science and Technology Research Institute (AMIRS-NSTRI) Karaj Iran Faculty of Pharmacy Islamic Azad University of Pharmaceutical Sciences Tehran Iran

In the present study various thiosemicarbazides and thiosemicarbazones were synthesized and screened for their antimicrobial activity. Among the ligands tested, compounds 1, 3, 4 and 5 demonstrated inhibitory effects at 0.4 - 0.5 μM concentrations against E. coli while 1-(4-fluorobenzoyl)-N4-ethyl-thiosemicar-bazide (3) demonstrated the maximum inhibitory effective compound against E. coli, also possessing high water solubility. Instead, compound 3 demonstrated weak antibiotic effects against S. aurous while compounds 4 - 6 demonstrated significant antibacterial effects at 0.4 - 0.42 μM against the latter organism. Chloramphenicol was used as the positive control.

关键词： Synthesis Antibacterial Thiosemicarbazides Thiosemi-carbazones

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