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Simultaneous determination of 14 nitroimidazoles using thin-layer chromatography combined with surface-enhanced Raman spectroscopy(TLC-SERS)

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Food Bioscience 2022年第4期48卷 310-319页

作者： Siqian Shi Hang Yu fangwei yang Weirong Yao Yunfei Xie State Key Laboratory of Food Science and Technology Jiangnan UniversityChina School of Food Science and Technology Jiangnan UniversityChina Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province Jiangnan UniversityNo.1800 Lihu AvenueWuxi214122Jiangsu ProvinceChina

Nitroimidazoles are a family of veterinary drugs containing a 5-nitroimidazole ring structure,which are commonly used *** view of their potential genotoxicity and possible coexistence in actual samples,it is necessary to establish a rapid and effective method for the simultaneous determination of multiple *** this study,a rapid thin-layer chromatography combined with surface-enhanced Raman spectroscopy(TLC-SER)detection method for 14 nitroimidazole compounds has been *** theoretical Raman spectra of the nitroimidazoles were calculated using the B3LYP/6-311+G(d)basis set in density functional theory(DFT)and the theoretical Raman spectra of TNZ,NBZ,ONZ,SCZ,DMZ,MNI,2-HMZ,4-HMZ,CMNI,IPZ,MNZ-OH and HMDMZ have been obtained for the first *** detection was carried out on a gold nanoparticles(AuNPs)substrate,which exhibited strong SERS signals and a limit of detection limit of 0.1 mg/*** optimizing the conditions used for the developing agent in TLC,12 nitroimidazoles were successfully separated,while MNZ and RNZ still could not be *** combined with the chemometrics analysis method,the TLC-SERS method can realize the simultaneous detection of 14 *** pork samples were spiked with nitroimidazoles with a recovery rate of 80%-110%and RSD of 0.74%-16.70%,which showed that SERS and TLC-SERS methods are also effective for detection in actual *** detection time of this method is<10 min,which is suitable for the rapid detection of nitroimidazoles,especially for the rapid qualitative identification of nitroimidazoles in situ.

关键词： Nitroimidazoles SERS TLC-SERS PCA

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Establishment of the thin-layer chromatography-surface-enhanced Raman spectroscopy and chemometrics method for simultaneous identification of eleven illegal drugs in anti-rheumatic health food

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Food Bioscience 2022年第5期49卷 556-568页

作者： fangwei yang Cheng Wang Hang Yu Yahui Guo Yuliang Cheng Weirong Yao Yunfei Xie State Key Laboratory of Food Science and Technology Jiangnan UniversityNo.1800 Lihu AvenueWuxi214122Jiangsu ProvinceChina School of Food Science and Technology Jiangnan UniversityNo.1800 Lihu AvenueWuxi214122Jiangsu ProvinceChina Joint International Research Laboratory of Food Safety Jiangnan UniversityNo.1800 Lihu AvenueWuxi214122Jiangsu ProvinceChina

The risk of the illegal addition of anti-inflammatory and analgesic chemical drugs in anti-rheumatic health foods should not be *** supervision and rapid on-site detection technology need to be ***-layer chromatography-surface-enhanced Raman spectroscopy(TLC-SERS),which has the advantages of simple operation,fast separation,and qualitative and quantitative detection,was used in this *** these eleven chemical drugs(acetaminophen,acetylsalicylic acid,aminophenazone,dexamethasone,diclofenac sodium,hydrocortisone,indometacin,naproxen,phenylbutazone,piroxicam,prednisone 21-acetate)that may be added to anti-rheumatic health foods have been simultaneously identified by TLC-SERS combined with chemometrics *** characteristic signals of the separated drug spots were collected by SERS,which were optimized by the gold colloidal nanoparticles’volume and integration *** SERS was subjected to principal component analysis(PCA)to reduce dimensionality and combined with the pattern recognition methods in chemometrics,such as PCA-Linear Discriminant Analysis(LDA),PCA-K Nearest Neighbor and PCA-Support Vector Machine,and eleven drug components were judged and ***,the predictive performances of different models were also analyzed and *** results showed that the TLC plate and four organic solvents of petroleum ether,chloroform,ethyl acetate and acetic acid were selected as the developing *** dropping amount of gold colloidal nanoparticles and the integration time were set and *** limit of detection of the simultaneous detection method of SERS was 10-100 mg/***,SERS was preprocessed by Gap-Segment 2nd Derivative,and then the PCA-LDA model was established,and the model’s prediction accuracy can reach 100%.The method is simple,rapid,sensitive and accurate,and has low experimental instruments and equipment *** is suitable for the on-site simultaneous detection of various anti-inflammato

关键词： TLC-SERS Health food Illegal drugs Chemometrics Simultaneous identification Rapid detection

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Trans-cinnamaldehyde inhibits Penicillium italicum by damaging mitochondria and inducing apoptosis mechanisms

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Food Science and Human Wellness 2022年第4期11卷 975-981页

作者： fangwei yang Jiaqi Mi Fei Huang Prompong Pienpinijtham Yahui Guo Yuliang Cheng Weirong Yao Yunfei Xie School of Food Science and Technology Jiangnan UniversityWuxi 214122China State Key Laboratory of Food Science and Technology Jiangnan UniversityWuxi 214122China Qingdao Special Food Research Institute Qingdao 266109ShandongChina

Plant-derived essential oils have excellent antifungal effects and can be used for the preservation of fresh foods such as fruits and vegetables, but the detailed mechanism has not been fully elucidated. In this study, we investigated the inhibitory effects of trans-cinnamaldehyde on Penicillium italicum, a common pollution fungus in citrus, and explored the antifungal mechanism of trans-cinnamaldehyde by detecting fungal oxidative damage, mitochondrial metabolism, and cell apoptosis. These results showed that transcinnamaldehyde made the carboxylic acid cycle deregulated by altering the related enzyme activities(succinate dehydrogenase, malate dehydrogenase) and mid product. Moreover, the level of reactive oxygen species rose sharply while the redox level was out of regulation. The mitochondrial membrane potential collapsed, leading to the leakage of cytochrome c, and then triggering the activation of apoptotic protease, which was further confirmed by the significant increase in caspase-3 activity from(3.6 ± 0.6) U to(8.8 ± 1.1) U(P < 0.05). The cytochrome c in mitochondria was detected by confocal Raman microspectroscopy, the characteristic intensity index(I750/I2944) was decreased, indicating that the cytochrome c in mitochondria was reduced and leakage. Besides, the strong negative correlation between Raman intensity and the amount of cytochrome c leakage was established with the correlation coefficient of-0.981 7. This study revealed that destroying the integrity of the mitochondrial membrane, activating the mitochondrial-mediated apoptosis pathway was the in-depth antifungal mechanism of trans-cinnamaldehyde;and Raman spectroscopy technology provided new ideas to study this process with high sensitivity determination of cytochrome c.

关键词： trans-cinnamaldehyde Penicillium italicum Raman microspectroscopy Inhibition mechanism Cytochrome c Apoptosis

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A novel malachite green staining approach for evaluating the internal freshness of egg by detecting the quality of egg cuticle

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Food Bioscience 2022年第6期50卷 814-822页

作者： Zixuan Xu Yuliang Liu fangwei yang Lijun Huang Hang Yu Weirong Yao Yunfei Xie State Key Laboratory of Food Science and Technology Jiangnan University1800 Lihu AvenueWuxiJiangsu214122China School of Food Science and Technology Jiangnan UniversityNo.1800 Lihu AvenueWuxi214122Jiangsu ProvinceChina Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province Jiangnan UniversityNo.1800 Lihu AvenueWuxi214122Jiangsu ProvinceChina Wuxi Food Safety Inspection and Test Center 35-210 Changjiang South RoadWuxi214142Jiangsu ProvinceChina

The cuticle is the outermost protective film of eggs, whose quality will affect internal freshness. At present, the undamaged detection of eggs freshness relies on hyperspectral imaging technology, which is relatively complicated. In this study, a novel simple approach for evaluating internal freshness of eggs was further explored by detecting the quality of egg cuticle. Malachite green selected as the alternative dye for quality evaluation of adhesive film, the correlation analysis was carried out by collecting the physical and chemical indexes of eggs with different degrees of freshness. It is found that there was a strong positive correlation between the quality of all parts of the cuticle and internal freshness (p < 0.01), and the Pearson correlation coefficient was over 0.85. The Haugh unit and pH of albumen were most strongly correlated with cuticle quality at the top, with the correlation coefficient of 0.919 and −0.906, respectively. The cuticle quality of eggs with contents got worse than that of eggs without contents, and the holes increased when they were stored for 28 days. In this way, malachite green staining method can be used to identify the freshness of egg cuticle, and the quality of egg cuticle can be used to distinguish eggs with different freshness. This study provided a new direction for the study of egg freshness and assisted to distinguish the quality and safety of eggs nondestructively.

关键词： Cuticle Egg freshness Bidirectional effect Quality evaluation Correlation analysis

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脱落酸的生物合成及对水果成熟的调控研究进展

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食品科学 2016年第3期37卷 266-272页

作者：杨方威段懿菲冯叙桥渤海大学食品科学与工程学院辽宁省食品安全重点实验室生鲜农产品贮藏加工及安全控制技术国家地方联合工程研究中心辽宁锦州121013

作为一种重要的植物激素,脱落酸(abscisic acid,ABA)除具有引起植物芽体休眠、叶子脱落、气孔关闭、抑制植株生长和增强植物抗逆性等生理作用外,在果实生长发育和成熟衰老的过程中也具有重要的调控作用。高等植物组织中ABA生物合成主要... 详细信息

作为一种重要的植物激素,脱落酸(abscisic acid,ABA)除具有引起植物芽体休眠、叶子脱落、气孔关闭、抑制植株生长和增强植物抗逆性等生理作用外,在果实生长发育和成熟衰老的过程中也具有重要的调控作用。高等植物组织中ABA生物合成主要是通过C40类胡萝卜素的间接途径来实现的。植物内源ABA的含量水平处于动态平衡状态,受其生物合成和分解代谢等因素影响,在此过程中有关基因转录表达水平和酶活性起着关键作用。内源ABA含量的升高会导致水果成熟和果肉软化,ABA可能同时调控了呼吸跃变型和呼吸非跃变型水果的成熟衰老。探究ABA的生物合成及其调控作用,对揭示水果的生长发育、品质形成、着色软化、成熟衰老等过程具有重要的现实意义。本文综述了ABA及其生成代谢相关基因调控水果成熟的研究进展,并展望了ABA在提高水果品质、延长水果货架期等方面的商业应用。

关键词：脱落酸生物合成水果成熟调控

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适宜冻结温度保持牛肉蛋白稳定性抑制水分态变

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农业工程学报 2015年第23期31卷 238-245页

作者：李侠孙圳杨方威张春晖谢小雷中国农业科学院农产品加工研究所/农业部农产品加工综合性重点实验室北京100193

为进一步明确肉品工业中不同冷冻温度对肉品品质的影响,探究了-18、-23及-38℃冻结后解冻(4℃)对牛肉蛋白稳定性及水分态变的影响。采用差示扫描量热(differential scanning calorimeter,DSC)、傅里叶变换红外光谱(Fourier transform in... 详细信息

为进一步明确肉品工业中不同冷冻温度对肉品品质的影响,探究了-18、-23及-38℃冻结后解冻(4℃)对牛肉蛋白稳定性及水分态变的影响。采用差示扫描量热(differential scanning calorimeter,DSC)、傅里叶变换红外光谱(Fourier transform infrared,FTIR)、低场核磁共振波谱(low filed nuclear magnetic resonance,LF-NMR)及H质子成像(magnetic resonance imaging,MRI)等技术,对比分析了-18、-23及-38℃冻结后解冻(4℃)过程中牛肉冻结—解冻曲线、蛋白质热稳定性、蛋白质二级结构、蛋白质表面疏水性、水分态变、解冻汁液流失等指标,研究了3种温度条件下冻结—解冻过程中蛋白质稳定性与水分态变规律。结果表明,牛肉的冻结点温度为-1.1℃,冻结温度越低,通过最大冰晶生成带所用的时间就越短,而肉样解冻至中心温度为4℃时所需时间也越长;-38℃冻结过程中肌原纤维蛋白的抗变性能力与热稳定性较好;冻结后牛肉肌原纤维蛋白发生部分程度的变性,而解冻后牛肉蛋白又会发生复性;冻结导致牛肉蛋白质表面疏水性显著升高(P<0.05),而解冻后牛肉蛋白质表面疏水性显著降低(P<0.05)。FTIR、LF-NMR及MRI等结果相互佐证,表明-38℃冻结牛肉蛋白质二级结构的稳定性最好,解冻后蛋白质复性、水分回吸,不易流动水态变成自由水的趋势显著低于-23和-18℃冻结(P<0.05),解冻汁液流失也最少(P<0.05)。试验结果揭示了冻结温度对牛肉蛋白质稳定性及水分态变的影响。

关键词：肉冷冻温度蛋白质稳定性水分态变

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小麦胚的稳定化处理方法

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粮食与食品工业 2005年第5期12卷 13-14页

作者：杨芳伟安徽省东方面粉厂宿州234000

介绍了小麦胚不宜储存的原因及6种稳定化处理方法,并介绍了安徽省东方面粉厂的成功经验。

关键词：小麦胚稳定化

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浅谈小麦胚的储藏

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面粉通讯 2006年第2期 43-45页

作者：杨芳伟安徽省东方面粉厂 234000

1小麦胚的储藏问题应该引起重视小麦胚芽占小麦的2%～3%,含有蛋白质、脂肪和不饱和脂肪酸,多种维生素、矿物质和各种氨基酸,特别是含有维生素E和赖氨酸等,具有较高的营养价值.

关键词：小麦胚芽储藏不饱和脂肪酸维生素E 营养价值蛋白质氨基酸矿物质赖氨酸

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与己醛具有群体感应协同抑制效应的精油成分组合筛选研究

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食品科学技术学报 2022年第2期40卷 72-81页

作者：刘宇于航谢云飞杨方威姚卫蓉江南大学食品学院江苏无锡214122

为了减少精油作为抗菌剂的实际使用量,研究了对胡萝卜软腐欧氏杆菌(蔬菜腐败菌)的群体感应具有协同抑制作用的精油成分组合。基于胡萝卜软腐欧氏杆菌的最小抑菌浓度和分级抑菌浓度,从9种精油成分中筛选出与己醛具有协同抑菌作用的4个精... 详细信息

为了减少精油作为抗菌剂的实际使用量,研究了对胡萝卜软腐欧氏杆菌(蔬菜腐败菌)的群体感应具有协同抑制作用的精油成分组合。基于胡萝卜软腐欧氏杆菌的最小抑菌浓度和分级抑菌浓度,从9种精油成分中筛选出与己醛具有协同抑菌作用的4个精油成分组合,利用紫色杆菌CV026和胡萝卜软腐欧氏杆菌筛选其中对二者群体感应具有协同抑制效应的精油成分组合,进一步利用能够判别两种物质是否具有协同作用的金氏公式验证,发现己醛只有与香叶醇组合才具有较好的群体感应协同抑制效果,而且有效浓度仅为己醛和香叶醇单体最小抑菌浓度的1/8和1/16。该组合对紫色杆菌CV026和胡萝卜软腐欧氏杆菌的群体感应相关指标均具有较好的抑制效果,其中群体感应指标中的产紫色菌素、游泳运动、群集运动、产胞外多糖和生物被膜形成的抑制率分别为52.91%、58.19%、51.49%、65.03%和69.84%。通过扫描电子显微镜和激光共聚焦显微镜进一步观察到己醛联合香叶醇对胡萝卜软腐欧氏杆菌生物被膜的形成具有明显的破坏作用。研究结果表明,具有协同抑制腐败菌群体感应作用的己醛与香叶醇组合,可以在降低精油成分实际使用量的同时,有效抑制腐败菌的腐败特性。

关键词：群体感应协同抑制精油成分己醛香叶醇胡萝卜软腐欧氏杆菌

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浅谈小麦高免疫生物活性肽的提取工艺

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现代面粉工业 2011年第1期25卷 40-43页

作者：杨芳伟安徽省东方面粉厂安徽宿州234000

研究了以小麦蛋白为原料,通过酶解技术制备具有生物活性的小肽,再用离子交换与分子筛技术进行提纯,形成小麦高免疫生物活性肽提取工艺与技术。

关键词：小麦蛋白酶解离子交换分子筛技术活性肽

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