检索结果-南通市图书馆

番茄抗坏血酸含量相关QTL定位及候选基因鉴定

在线全文

学校读者我要写书评

暂无评论

园艺学报 2024年第2期51卷 219-228页

作者：刘根忠李方曼葛平飞陶金宝张星雨叶志彪张余洋山东农业大学园艺科学与工程学院山东泰安271018 华中农业大学果蔬园艺作物种质创新与利用全国重点实验室武汉430070 湖北洪山实验室武汉430070 华中农业大学深圳营养与健康研究院武汉430070 中国农业科学院深圳农业基因组研究所岭南现代农业科学与技术广东省实验室深圳分中心广东深圳518000

以番茄果实高抗坏血酸含量材料TS-226和低抗坏血酸含量材料TS-228为试验亲本杂交和自交创制F2遗传分离群体,分别构建高抗坏血酸混池和低抗坏血酸混池,集团分离群体测序(bulked segregant analysis sequencing,BSA-seq)分析鉴定到与番茄... 详细信息

以番茄果实高抗坏血酸含量材料TS-226和低抗坏血酸含量材料TS-228为试验亲本杂交和自交创制F2遗传分离群体,分别构建高抗坏血酸混池和低抗坏血酸混池,集团分离群体测序(bulked segregant analysis sequencing,BSA-seq)分析鉴定到与番茄果实抗坏血酸相关的主效QTL,进一步将与抗坏血酸相关的候选基因定位于番茄基因组8号染色体58.00~60.15 Mb区域内。结合?SNP-index数据,推断SlPPO(Polyphenol oxidase)是参与调控番茄抗坏血酸含量的主效基因。以TS-228为背景材料,通过农杆菌介导方法进行遗传转化,获得SlPPO过表达和沉默株系,相比野生型,过表达株系OE-3和OE-18的红熟果实总抗坏血酸含量分别减少了18.89%和26.56%;沉默株系Ri-9和Ri-16红熟果实总抗坏血酸含量分别增加了37.53%和63.93%。综上所述,SlPPO为参与控制番茄红熟果实抗坏血酸含量的关键基因,对果实抗坏血酸含量起负向调控作用。

关键词：番茄集团分离群体测序抗坏血酸 QTL SlPPO

A 21-bp InDel in the promoter of STP1 selected during tomato improvement accounts for soluble solid content in fruits

在线全文

学校读者我要写书评

暂无评论

Horticulture Research 2023年第3期10卷 176-188页

作者： Ying Wang Chunmei Shi Pingfei Ge fangman li lihui Zhu Yaru Wang Jinbao Tao Xingyu Zhang Haiqiang Dong Wenxian Gai Fei Wang Zhibiao Ye Donald Grierson Wei Xu Yuyang Zhang National Key Laboratory for Germplasm Innovation and Utilization of Horticultural Crops College of Horticulture and Forestry ScienceHuazhong Agricultural UniversityWuhan 430070China Plant Sciences Division School of BiosciencesUniversity of NottinghamSutton Bonington CampusLoughborough LE125RDUK Key Laboratory of Special Fruits and Vegetables Cultivation Physiology and Germplasm Resources Utilization(Xinjiang Production and Construction Crops) College of AgricultureShihezi UniversityShihezi 832003XinjiangChina Hubei Hongshan Laboratory Wuhan 430070China

Domestication and improvement are important processes that generate the variation in genome and phonotypes underlying crop ***,during selection for certain attributes,other valuable traits may be inadvertently *** example is the decline in fruit soluble solids content(SSC)during tomato *** genetic loci for SSC have been identified,but few reports on the underlying mechanisms are *** this study we performed a genome-wide association study(GWAS)for SSC of the red-ripe fruits in a population consisting of 481 tomato accessions with large natural variations and found a new quantitative trait locus,STP1,encoding a sugar transporter *** causal variation of STP1,a 21-bp InDel located in the promoter region 1124 bp upstream of the start codon,alters its ***1 Insertion accessions with an 21-bp insertion have higher SSC than STP1Deletion accessions with the 21-bp *** of STP1 in TS-23 with high SSC using CRISPR/Cas9 greatly decreased SSC in *** vivo and in vitro assays demonstrated that ZAT10-liKE,a zinc finger protein transcription factor(ZFP TF),can specifically bind to the promoter of STP1Insertion to enhance STP1 expression,but not to the promoter of STP1Deletion,leading to lower fruit SSC in modern *** analysis revealed that STP1 was selected during tomato *** these results together,we identified a naturally occurring causal variation underlying SSC in tomato,and a new role for ZFP TFs in regulating sugar *** findings enrich our understanding of tomato evolution and domestication,and provide a genetic basis for genome design for improving fruit taste.

关键词： CRISPR/Cas9 content sugar

A CCAAT-binding factor,SlNFYA10,negatively regulates ascorbate accumulation by modulating the D-mannose/L-galactose pathway in tomato

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Horticulture Research 2020年第1期7卷 141-152页

作者： Weifang Chen Tixu Hu Jie Ye Bing Wang Genzhong liu Ying Wang Lei Yuan Jiaming li fangman li Zhibiao Ye Yuyang Zhang Key Laboratory of Horticultural Plant Biology Ministry of EducationHuazhong Agricultural University430070 WuhanChina HZAU Chuwei Institute of Advanced Seeds 430070 WuhanChina

Ascorbic acid(AsA),an important antioxidant and growth regulator,and it is essential for plant development and human ***,humans have to acquire AsA from dietary sources due to their inability to synthesize *** AsA biosynthesis pathway in plants has been elucidated,but its regulatory mechanism remains largely *** this report,we biochemically identified a CCAAT-box transcription factor(SlNFYA10)that can bind to the promoter of SlGME1,which encodes GDP-Man-3’,5’-epimerase,a pivotal enzyme in the D-mannose/L-galactose ***,SlNFYA10 simultaneously binds to the promoter of SlGGP1,a downstream gene of SlGME1 in the Dmannose/L-galactose *** assays in yeast and functional analyses in plants have confirmed that SlNFYA10 exerts a negative effect on the expression of both SlGME1 and *** tomato lines overexpressing SlNFYA10 show decreased levels of SlGME1 and SlGGP1 abundance and AsA concentration in their leaves and fruits,accompanied by enhanced sensitivity to oxidative ***,SlNFYA10 is the first CCAAT-binding factor identified to date to negatively regulate the AsA biosynthetic pathway at multiple sites and modulate plant responses to oxidative stress.

关键词： dietary oxidative expressing

Leaching kinetics of fluorine during the aluminum removal from spent li-ion battery cathode materials

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Environmental Sciences 2024年第4期138卷 312-325页

作者： Shengjie li Jianxin Zhu Research Center for Eco-Environmental Sciences Chinese Academy of SciencesBeijing 100085China University of Chinese Academy of Sciences Beijing 100049China

The high content of aluminum(Al)impurity in the recycled cathode powder seriously affects the extraction efficiency of Nickel,Cobalt,Manganese,and lithium resources and the actual commercial value of recycled materials,so Al removal is crucially important to conform to the industrial standard of spent li-ion battery cathode *** this work,we systematically investigated the leaching process and optimum conditions associated with Al removal from the cathode powder materials collected in a wet cathode-powder peeling and recycling production line of spent li-ion batteries(liBs).Moreover,we specifically studied the leaching of fluorine(F)synergistically happened along with the removal process of Al,which was not concerned about in other studies,but one of the key factors affecting pollution prevention in the recovery *** mechanism of the whole process including the leaching of Al and F from the cathode powder was indicated by using NMR,FTIR,and XPS,and a defluoridation process was preliminarily investigated in this *** leaching kinetics of Al could be successfully described by the shrinking core model,controlled by the diffusion process and the activation energy was 11.14 kJ/***,the leaching of F was attributed to the dissolution of liPF6and decomposition of PVDF,and the kinetics associated was described by Avrami *** interaction of Al and F is advantageous to realize the defluoridation to some *** is expected that our investigation will provide theoretical support for the large-scale recycling of spent liBs.

关键词： Al removal F leaching Cathode powder Heating alkali leaching Leaching kinetics

Additives strategies to improving cycling stability of li metal batteries by modifying electrode electrolyte interphases

在线全文

学校读者我要写书评

暂无评论

Progress in Natural Science:Materials International 2024年第2期34卷 338-344页

作者： Yuan li Pengfei Huang Chunyu Cui Xiaoyuan Ma li Chen Zekun li linhai li Yuhong Tian School of Biological and Chemical Engineering Guangxi University of Science and Technology School of College of Chemistry and Chemical Engineering Hunan University Isky Future Energy Institute

li metal batteries（LMBs） have attracted much attention due to the ultra-high theoretical capacity of the li metal anode（LMA）.However,the uncontrollable dendrites growth and low coulombic efficiency hinder their practical ***,we explore 3,5-difluoropyridine（2F-BD） as a novel electrolyte additive to enable highperformance *** 2F-BD additive participates in and modifies the solvation structure of li ions by reducing the coordinated number of PF6-at the electrode surface,leading to the formation of a liF and li3N-rich solid electrolyte interphase（SEI）.The liF component increases the robustness of SEI and suppresses the formation of li dendrites,while the li3N component facilitates the transportation and reaction kinetics of li *** a result,the li‖li symmetric cell presents a stable cycling performance of up to 500 h at a current density of 1 mA *** coupling with liFePO4cathode,the obtained full cell achieves high specific capacities of 106.06 mA h g-1and 84.98 mA h g-1at 2.55 mA cm-2and 5.10 mA cm-2,respectively,and maintains a high capacity retention of84.0% after 1000 cycles at 0.13 mA cm-2,with an average cycling CE of 99.58%.

关键词： Electrolyte additive li dendrites li metal batteries liF and li3N-Rich electrode-electrolyte interphase liFePO4 cathode

Multiple-model GWAS identifies optimal allelic combinations of quantitative trait loci for malic acid in tomato

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Horticulture Research 2023年第4期10卷 23-37页

作者： Wenxian Gai Fan Yang liangdan Yuan Saeed ul Haq Yaru Wang Ying Wang Lele Shang fangman li Pingfei Ge Haiqiang Dong Jinbao Tao Fei Wang Xingyu Zhang Yuyang Zhang National Key Laboratory for Germplasm Innovation and Utilization of Horticultural Crops Huazhong Agricultural UniversityWuhan 430070China Hubei Hongshan Laboratory Wuhan 430070China College of Horticulture Northwest A&F UniversityYangling 712100China Department of Horticulture The University of Agriculture PeshawarPeshawar 25130Pakistan

Malic acid(MA)is an important flavor acid in fruits and acts as a mediator in a series of metabolic *** is important to understand the factors affecting MA metabolism for fruit flavor improvement and to understand MA-mediated biological ***,themetabolic accumulation of MA is controlled by complex heredity and environmental factors,making it difficult to predict and regulate the metabolism of *** this study,we carried out a genome-wide association study(GWAS)on MA using eight milestone models with two-environment repeats.A series of associated SNP variations were identified from the GWAS,and 15 high-confidence annotated geneswere further predicted based on linkage disequilibrium and lead *** transcriptome data of candidate geneswere explored within different tomato organs as well as various fruit tissues,and suggested specific expression patterns in fruit *** on the genetic parameters of population differentiation and SNP distribution,tomato MA content has been more influenced by domestication sweeps and less affected by improvement sweeps in the long-term history of tomato *** addition,genotype×environment interaction might contribute to the difference in domestication phenotypic data under different *** study provides new genetic insights into how tomato changed its MA content during breeding and makes available function-based markers for breeding by marker-assisted selection.

关键词： breeding GWAS metabolism

SEI/dead li-turning capacity loss for high-performance anode-free solid-state lithium batteries

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Energy Chemistry 2024年第9期96卷 145-152页

作者： Qianwen Yin Tianyu li Hongzhang Zhang Guiming Zhong Xiaofei Yang Xianfeng li Division of Energy Storage Dalian National Laboratory for Clean EnergyDalian Institute of Chemical PhysicsChinese Academy of SciencesDalian 116023LiaoningChina University of Chinese Academy of Sciences Beijing 100049China Key Laboratory of Long-Duration and Large-Scale Energy Storage Dalian Institute of Chemical PhysicsChinese Academy of SciencesDalian 116023LiaoningChina Laboratory of Advanced Spectro-Electrochemistry and lithium-Ion Batteries Dalian National Laboratory for Clean EnergyDalian Institute of Chemical PhysicsChinese Academy of SciencesDalian 116023LiaoningChina

Anode-free solid-state lithium metal batteries(AF-SSLBs)have the potential to deliver higher energy density and improved safety beyond lithium-metal ***,the unclear mechanism for the fast capacity decay in AF-SSLBs,either determined by dead li or solid electrolyte interface(SEI),limits the proposal of effective strategies to prolong cycling *** clarify the underlying mechanism,herein,the evolution of SEI and dead li is quantitatively analyzed by a solid-state nuclear magnetic resonance(ss-NMR)technology in a typical liPF6-based polymer *** results show that the initial capacity loss is attributed to the formation of SEI,while the dead li dominates the following capacity loss and the growth rate is 0.141 mA h cm^(−2)cycle−*** reduce the active li loss,the combination of inorganic-rich SEI and self-healing electrostatic shield effect is proposed to improve the reversibility of li deposition/dissolution behavior,which reduces the capacity loss rate for the initial SEI and following dead li generation by 2.3 and 20.1 folds,*** a result,the initial Coulombic efficiency(ICE)and stable CE increase by 15.1%and 15.3%in li-Cu cells,which guides the rational design of high-performance AF-SSLBs.

关键词： Solid-state lithium batteries Solid-state NMR Anode-free SEI Dead li

Synergy effect of regulated li-plating and functional solid electrolyte interphase on graphite anodes

在线全文

学校读者我要写书评

暂无评论

Nano Research 2024年第9期17卷 8077-8085页

作者： Panpan Wang Baojia Xia Jianling li State Key Laboratory of Advanced Metallurgy University of Science and Technology BeijingBeijing 100083China Shanghai Institute of Microsystem and Information Technology Chinese Academy of SciencesShanghai 201800China

The growth of li dendrites poses potential safety hazard to lithium-ion batteries(liBs),and eliminating li dendrites thoroughly stills face tough difficulties ***,regulating li-plating is a critical optimization-direction to address the ***,a“graphite-li hybrid”anode with high reversibility is realized under the constant-capacity lithiation(CCL).Within CCL,the uniform distribution of li-plating on the graphite surface is successfully *** evolution in different states of solid electrolyte interphase(SEI)is investigated in detail to study the interaction between the potentials and impedance during the process of liintercalation and *** the potential below 0 V and the state of charge(SOC)of 110%relative to the theoretical capacity,the F-rich SEI with high stability is constructed to hinder the emergency of li dendrites and maintain the intact structure of graphite anode under long *** cell presents more than 100%Coulombic efficiency(CE)with the 900 cycles,demonstrating the reversible li-plating and the utilization of *** the CCL half-cell provides a good cycling performance and specific capacity of 900 cycles at 0.5 C,it is attributed to the synergy effect of stable inorganic-rich SEI and regulated active li-plating.

关键词： "graphite-li hybrid"anode li-plating reversibility solid electrolyte interphase(SEI)

Highly-chlorinated inert and robust interphase without mineralization of oxide enhancing high-rate li metal batteries

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Chinese Chemical Letters 2024年第6期35卷 239-243页

作者： Long li Kang Yang Chenpeng Xi Mengchao li Borong li Gui Xu Yuanbin Xiao Xiancai Cui Zhiliang liu lingyun li Yan Yu Chengkai Yang Key Laboratory of Advanced Materials Technologies International(HongKong Macao and Taiwan)Joint Laboratory on Advanced Materials TechnologiesSchool of Materials Science and EngineeringFuzhou UniversityFuzhou 350108China College of Material Sciences and Chemical Engineering Harbin Engineering UniversityHarbin 150001China

Side reactions and dendrite growth triggered by the unstable interface and inhomogeneous deposition have become the biggest obstacle to the commercialization for lithium metal *** this study,a highly-chlorinated organic-inorganic hybrid interfacial protective layer is developed by rationally tuning the interfacial passivation and robustness to achieve the convenient and efficient li metal *** polyvinyl chloride(PVC)can effectively resist water and oxygen,which is confirmed by density functional *** organic-dominant solid electrolyte interphases(SEI)with lithium chloride are investigated by the X-ray photoelectron spectroscopy(XPS)with little mineralization of oxide,such as li_(2)O and li_(2)CO_(3).With such artificial SEI,a uniform and dense lithium deposition morphology are formed and an ultra-long stable cycle of over 500 h are achieved even at an ultra-high current density of 10 m A/cm^(2).Moreover,the simple and convenient protected anode also exhibits excellent battery stability when paired with the liNi_(0.8)Co_(0.1)Mn_(0.1)O_(2)(NCM811)and liFePO_(4)(LFP)cathode,showing great potential for the commercial application of lithium metal batteries.

关键词： Highly-chlorinated Water/oxygen resistance Stable interphase Dendrite-free lithium metal batteries High-rate