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A Smartphone-assisted Paper-based Analytical Device for Fluorescence Assay of Hg2+

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Chemical Research in Chinese Universities 2019年第6期35卷 972-977页

作者： YE Jiavven GENG Yijia CAO fanghao SUN Dan XU Shuping CHANG Jingjing XU Weiqing chen Qidan State Key Laboratory of Supramolecular Structure and Materials Institute of Theoretical ChemistryJilin UniversityChangchun 130012P.R.China School of Chemistry and Environmental Engineering Changchun University of Science and TechnologyChangchun 130022P.R.China School of Chemical Engineering and New Energy Materials Zhuhai CollegeJilin UniversityZhuhai 519041P.R.China

Rapid,efficiency and portable detection systems in low-resource settings with limited laboratory equipment and technical expertise are urgently ***,an integrated platform composed of a paper-based analytical device and a smartphone detection system for Hg^2+ onsite testing was ***-doped carbon dots(N-CDs)were synthesized by a simple hydrothermal method using citric acid as the carbon source and ethanediamine as the nitrogen source,which gave out bright blue fluorescence under the excitation at 350 nm UV light and the absolute fluorescence quantum yield was 17.1%.The fluorescence of the prepared N-CDs can be effectively quenched by Hg^2+.In addition,an external attachment of smartphone for illumination and external light interference was designed to trace the fluorescence signals,and a software application of Android system with simple operation program was developed to perform snapshot and image *** smartphone-assisted detection system was combined with the N-CDs decorated paper chip to achieve the sensitive detection of Hg^2+ in water *** integrated method for reliable sensing of Hg^2+ shows a good linear detection range of 10–800μmol/L(R2=0.9595)with detection limit of 1.07×10^?8 mol/L.

关键词： Paper-based analytical device Nitrogen-doped carbon dot Integrated sensing platform Mercury ion

Oncolytic adenovirus targeting LASP-1 inhibited renal cell cancerprogression

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BIOCELL 2020年第4期44卷 639-647页

作者： fanghao SUN WENCHAO ZHAO LIANSHENG ZHANG HONGGUI MA JIAN ZHOU YOUGAN chen JIANQUAN HOU Department of Urology The First Affiliated Hospital of Soochow UniversitySuzhou215006China Department of Urology Soochow University Affiliated Wuxi Ninth HospitalWuxi214000China

Recent studies suggested that LIM and SH3 protein 1(LASP-1)is a promising therapeutic target for renal cell cancer(RCC).This study aimed to explore the role of LASP-1 in *** this purpose,LASP-1 expression in RCC tissues was analyzed by immunohistochemistry and Western blot *** proliferation,migration,invasion,and gene expression were detected by CCK-8 assay,Transwell assay,and Western blot *** results showed that LASP-1 was highly expressed in RCC,and its expression level,t was positively correlated with lymph node metastasis and tumor,nodes,and metastases(TNM)*** knockdown of LASP-1 expression significantly inhibited the proliferation of RCC cells,increased the apoptosis rate,and inhibited RCC cell invasion and migration by inhibiting epithelial–mesenchymal *** conclude that LASP-1 promotes RCC progression and metastasis and is a promising therapeutic target for RCC.

关键词： LASP-1 Oncolytic adenovirus Renal cell cancer

Differentiation trajectories and biofunctions of symbiotic and un-symbiotic fate cells in root nodules of Medicago truncatula

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Molecular Plant 2022年第12期15卷 1852-1867页

作者： Qinyi Ye Fugui Zhu fanghao Sun Tai-cheng Wang Jiale Wu Peng Liu chen Shen Jiangli Dong Tao Wang State Key Laboratory of Agrobiotechnology College of Biological SciencesChina Agricultural UniversityBeijing 100193China College of Grassland Science and Technology China Agricultural UniversityBeijing 100193China

The root nodule is a complex symbiotic nitrogen fixation factory,in which cells are highly ***,the differentiation trajectories and interconnection of nodule cells remain largely *** this study,we set up a modified protocol for nodule protoplast preparation and performed a single-cell RNA sequencing profiling of the indeterminate Medicago truncatula *** designated 13 cell clusters with specific expression patterns in 14-day post inoculation nodules and constructed a spatial and functional cellular map based on experimental data and bioinformatic *** analysis further revealed that two groups of apical meristematic cells develop into symbiotic and un-symbiotic fate cells along their particular *** analysis of each cell cluster revealed their particularity and interrelation,especially that the un-infected cells in nitrogen fixation zone are also involved in nitrogen assimilation by undertaking the asparagine ***,our data offer an important resource for investigating the mechanism of nodule organogenesis and symbiotic nitrogen fixation.

关键词： single-cell RNA sequencing scRNA-seq nodule organogenesis symbiotic nitrogen fixation indeterminate nodule legume plant

Polythiophene Derivatives Carbonized Polymer Dots:Aggregation Induced Solid-State Fluorescence Emission

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Chinese Journal of Chemistry 2023年第16期41卷 1950-1956页

作者： Jian Li fanghao Zhao Fuchun Nan Jian Wang Yunxiu Zhang Ke Liang Xiaokuang Xue Tiejin chen Lin Kong Jiechao Ge Pengfei Wang Key Laboratory of Photochemical Conversion and Optoelectronic Materials and CityU-CAS Joint Laboratory of Functional Materials and Devices Technical Institute of Physics and ChemistryChinese Academy of SciencesBeijing 100049China The University of Manchester G.020 Chemistry Building M139PLManchesterUK School of Future Technology University of Chinese Academy of SciencesBeijing 100049China Binzhou Institute of Technology Weiqiao-UCAS Science and Technology ParkBinzhouShandong 256606China

Comprehensive Summary Currently,solid-state fluorescent carbonized polymer dots(CPDs)have attracted attention increasingly due to their applications for optoelectronic ***,designing CPDs possessing solid-state fluorescence and clarifying the fluorescence mechanism remain ***,we initially synthesized a novel type of polythiophene derivatives CPDs,poly-4,4’-(thiophene-3,4-diyl)dibenzoic acid carbonized polymer dots(PDBA-CPDs)with solid-state ***,the structural and optical characterization revealed that the solid-state fluorescence originated from the aggregation induced emission of the *** brief,in aggregation state,the remaining polymer structure groups on the surface of the CPDs overlapped and weakened the non-radiative transition,enhancing solid-state fluorescence ***,three polythiophene-derived CPDs were designed to further demonstrate the aggregation induced solid-state fluorescence ***,owing to their unique properties of solid-state fluorescence,the white LEDs(light emitting diodes)were fabricated with high color rendering index(CRI)of 82.7 and CIE coordinates of(0.37,0.39)using commercial 460 nm chip.

关键词： Carbonized polymer dots Aggregation induced emission Solid-state fluorescence Light-emitting devicesl Carbon Nanomaterials

Cancer-associated fibroblasts derived fibronectin extra domain A promotes sorafenib resistance in hepatocellular carcinoma cells by activating SHMT1

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Genes & Diseases 2024年第6期11卷 510-524页

作者： Yan Dong Yanrong chen Yijie Wang Xiang Zhao Ruiyang Zi Jie Hao Qiong Ding Haoran Jiang Xuesong Wang fanghao Lu Houjie Liang Zhihao Wei Jianjun Li Department of Oncology Southwest HospitalThird Military Medical University(Army Medical University)Chongqing 400038China Brain Research Center and State Key Laboratory of Trauma Burnsand Combined InjuryThird Military Medical University(Army Medical University)Chongqing 400038China

Resistance to sorafenib,an effective first-line treatment for advanced hepatocellular carcinoma(HCC),greatly compromised the prognosis of *** extracellular matrix is one of the most abundant components of the tumor *** acting as a physical barrier,it remains unclear whether cell interactions and signal transduction mediated by the extracellular matrix contribute to sorafenib *** the analysis of primary HCC organoid RNA-seq data combined with in vivo and in vitro experiments validation,we discovered that fibronectin extra domain A(FN-EDA)derived from cancer-associated fibroblasts played a critical role in sorafenib ***,FN-EDA stimulates the up-regulation of the key one-carbon metabolism enzyme SHMT1 in HCC cells via the TLR4/NF-κB signaling pathway,thereby countering the oxidative stress induced by ***,we reinforced the clinical significance of our discoveries by conducting in vivo assays with an immunodeficiency subcutaneous xenograft tumor model,which was established using primary cancer-associated fibroblasts derived from clinical HCC tissues,and through the analysis of HCC samples obtained from The Cancer Genome Atlas(TCGA)*** findings suggest that targeting the FN-EDA/SHMT1 pathway could be a potential strategy to improve sorafenib responsiveness in HCC patients.

关键词： Cancer-associated fibroblasts Fibronectin extra domain A Hepatocellular carcinoma Serine hydroxymethyl transferase1 Sorafenib resistance

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卫星推进系统自动TIG焊技术

焊接技术 2024年第12期53卷 74-78页

作者：陈芳浩付松松李志伟涂振北京控制工程研究所

卫星推进系统是卫星的重要组成部分，为卫星轨道机动、姿态调整及位置保持提供动力和转矩，该系统是通过管路及管路连接件将贮箱、自锁阀、过滤器、压力传感器、推力器等部件连接起来，形成一个密封的系统。因TIG焊具有热量集中、电弧... 详细信息

卫星推进系统是卫星的重要组成部分，为卫星轨道机动、姿态调整及位置保持提供动力和转矩，该系统是通过管路及管路连接件将贮箱、自锁阀、过滤器、压力传感器、推力器等部件连接起来，形成一个密封的系统。因TIG焊具有热量集中、电弧稳定、保护良好等优点，常被用于推进系统管路焊接。文中针对卫星推进系统钛合金管路TIG焊技术进行研究，介绍了手工氩弧焊和自动氩弧焊原理，分析了电弧电压、焊接电流、焊接速度等参数对焊接的影响，提出了卫星管路自动焊接工艺流程，并对焊接温度、热流、应力等进行了仿真计算，通过仿真固化了自动焊接参数。通过试验件的焊接、外观检测、焊缝X射线探伤及拉伸冲击试验，验证了提出的焊接工艺流程及仿真的工艺参数满足卫星推进系统管路TIG焊要求，能够用于卫星推进系统焊接。

关键词：卫星推进系统 TIG焊仿真

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First Imported Case of SARS-CoV-2 Omicron Subvariant BA.5—Shanghai Municipality,China,May 13,2022

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China CDC weekly 2022年第30期4卷 665-666页

作者： Hui Jiang Changcheng Wu Wenbo Xu Hongyou chen fanghao Fang Min chen Chaonan Wang Lingyue Yuan Huanyu Wu Zheng Teng Jinyuan Guo Yaxu Zheng Xihong Lyu Xuefei Qiao Guizhen Wu Wenjie Tan Xi Zhang Shanghai Municipal Center for Disease Control and Prevention ShanghaiChina National Institute for Viral Disease Control and Prevention China CDCBeijingChina Songjiang District Center for Disease Control and Prevention ShanghaiChina.

On April 27,2022,an international flight KL857 from Amsterdam,the Netherlands arrived at Pudong International Airport,Shanghai *** were transferred to the quarantine hotel for a routine 14-day medical observation in Songjiang District and regularly tested for severe acute respiratory syndrome coronavirus 2(SARS-CoV-2).One of the passengers(a 37-year-old Chinese male)was reported positive and diagnosed as a mild case on April *** case set out from Uganda(flight KL535)on April 25 and transferred at Amsterdam,the Netherlands on April 26 and Seoul,the Republic of Korea(KL857)on April *** patient has been vaccinated in four doses against coronavirus disease 2019(COVID-19)(Beijing Institute of Biological Products Co.,Ltd)in China and *** diagnosis,he was transferred to Shanghai Public Health Clinical Center for *** recovered after treatment and was discharged on May 12.

关键词： transferred routine doses

Virology features of a family cluster of SARS-CoV-2 infections in Shanghai,China

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Biosafety and Health 2021年第4期3卷 187-189页

作者： Yanqiu Zhou Zheng Teng Hongyou chen Xiaoxian Cui fanghao Fang Jiabin Mou Hui Jiang Xi Zhang Shanghai Municipal Center for Disease Control and Prevention Shanghai 200336China

The global spread of SARS-CoV-2 is currently continuing,and the World Health Organization has announced the risk assessment of the viruses as *** this study,we analyzed virology features of SARS-CoV-2 causing a family cluster *** the six family members,five have been laboratory-confirmed infection of SARS-CoV-2 viruses.A total of five SARS-CoV-2 viruses have been isolated from the nasopharyngeal *** complete genome of the viruses exhibited 100%nucleotide identity with each *** two nucleotide differences have been observed between genomes of the isolated viruses and the HCoV/Wuhan/IVDC-HB-01/2019 ***,SARS-CoV-2 has been confirmed as the causation of the family cluster infections.

关键词： SARS-CoV-2 Family cluster Nasopharyngeal swab Full genome sequencing

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四川盆地三叠纪蒸发岩盆地形成环境及白云岩储层

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石油实验地质 2010年第4期32卷 334-340,346页

作者：陈莉琼沈昭国侯方浩方少仙中国石油化工股份有限公司江苏油田分公司地质研究院江苏扬州225009 西南石油大学成都610500 中国石油天然气股份有限公司杭州石油地质研究院杭州310023

四川盆地中、下三叠统嘉陵江组二段—中三叠统雷口坡组厚达1 500 m以上,主要为白云岩、硬石膏岩和石盐岩,夹少量石灰岩地层组合,其中白云岩是盆地内天然气主产层之一。嘉陵江初期,四川盆地周边山系不断隆升,基底下陷东倾,周期性干热气... 详细信息

四川盆地中、下三叠统嘉陵江组二段—中三叠统雷口坡组厚达1 500 m以上,主要为白云岩、硬石膏岩和石盐岩,夹少量石灰岩地层组合,其中白云岩是盆地内天然气主产层之一。嘉陵江初期,四川盆地周边山系不断隆升,基底下陷东倾,周期性干热气候促使盆地由开放陆棚向局限盆地转变。嘉陵江早中期,干热气候使盆内海水浓缩,在建南及万县等地形较低处沉积白云岩—硬石膏岩地层。嘉陵江中期—雷口坡期,江南古陆急剧隆升、向西北挤压,七矅山、泸州—开江水下隆起相继形成,迫使盆地不断向西迁移,形成深坳陷高含盐盆地,并与外海隔绝,广泛沉积以白云岩—硬石膏岩—石盐岩为主的地层组合,局部见钙芒硝岩和杂卤石岩。白云岩储层除发育晶间孔外,亦有溶蚀孔隙,其上覆膏盐层是良好的区域盖层。

关键词：储层白云岩嘉陵江组雷口坡组三叠系蒸发岩盆地四川盆地

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