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A composite method for mapping quantitative trait loci without interference of female achiasmatic and gender effects in silkworm,Bombyx mori

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A composite method for mapping quantitative trait loci witho...

中国蚕学会第九届青年学术研讨会

作者： Chunlin Li Weidong Zuo Xiaoling Tong Hai Hu Liang Qiao Jiangbo Song Gao Xiong Rui Gao fangyin dai Cheng Lu State Key Laboratory of Silkworm Genome Biology Southwest University Key Laboratory of Sericulture biology and Genetic breeding Agricultural MinistryCollege of BiotechnologySouthwest University Institute of Entomology and Molecular Biology College of Life SciencesChongqing Normal University

The silkworm,Bombyx mori,is an economically important insect that was domesticated more than 5000 years *** major economic traits focused on by breeders are quantitative traits,and an accurate and efficient QTL mapping method is necessary to explore their genetic ***,current widely used QTL mapping models are not well suited for silkworm because they ignore female achiasmate and gender *** this study,we propose a composite method combining rational population selection and special mapping methods to map QTL in *** determining QTL for cocoon shell weight（CSW）,we demonstrated the effectiveness of this *** the CSW mapping process,only 56 markers were used and five loci or chromosomes were detected,more than in previous ***,loci on chromosomes 1 and 11 dominated and accounted for 35.10% and 15.03% of the phenotypic variance *** previous studies,epistasis was detected between loci on chromosomes 11 and *** mapping results demonstrate the power and convenience of this method for QTL mapping in silkworm,and this method may inspire the development of similar approaches for other species with special genetic characteristics.

关键词： Bombyx mori Quantitative trait loci mapping Female achiasmate gender effects Cocoon shell weight

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日本九州大学家蚕基因资源保存的几个特点

蚕学通讯 2001年第2期21卷 60-63页

作者：代方银西南农业大学蚕丝学院农业部蚕桑学重点实验室重庆400716

目前,世界家蚕遗传资源主要集中保存在中国和日本.日本九州大学大学院农学研究院基因资源开发研究中心家蚕基因开发部门和本研究室,是国际上进行突变系统保存研究的两大机构,二者所保存的家蚕突变系统综合计覆盖世界现存突变系统的90%以... 详细信息

目前,世界家蚕遗传资源主要集中保存在中国和日本.日本九州大学大学院农学研究院基因资源开发研究中心家蚕基因开发部门和本研究室,是国际上进行突变系统保存研究的两大机构,二者所保存的家蚕突变系统综合计覆盖世界现存突变系统的90%以上.两方的交流合作历史悠久,本室基因库先期的奠基人蒋同庆教授30年代即在九大留学深造多年.但二战后的近半个世纪,交流不幸被中断.其间,两者通过独自发展,逐步形成了在系统保存、遗传分析、开发利用等方面各有特色的格局.随着中日关系的正常化,两方的交流合作得以继续.尤其是双方1997年实施大学间国际学术合作研究项目,建立了紧密的合作关系,研究者的互访和研究情报的交流等得到更进一步的推动.现在,强化双方有关研究者、技术人员的交流,特别是青年主力人才的培养,对于妥善保存世界现存的贵重的家蚕遗传资源,以及确保其在基础生命科学研究和产业应用研究上的利用,促进家蚕遗传学、蚕丝学和蚕丝业的发展,无疑是当前极其重要的措施和面对未来最负责的态度.

关键词：日本九州大学家蚕基因资源保存特点遗传

维普期刊数据库

Hippo pathway regulates somatic development and cell proliferation of silkworm

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Hippo pathway regulates somatic development and cell prolife...

中国蚕学会第十四届暨国家蚕桑产业技术体系家（柞）蚕遗传育种学术研讨会

作者： Niannian Li Xiaoling Tong Jie Zeng Gang Meng Fuze Sun Hai Hu Jiangbo Song Cheng Lu fangyin dai State Key Laboratory of Silkworm Genome Biology Key Laboratory for Sericulture Functional Genomics and Biotechnology of Agricultural Ministry Southwest University

Hippo signaling pathway(signaling pathway Hippo, hereinafter referred to as the Hippo pathway) was the earliest found in Drosophila, which can regulate the development of tissues and organs, even some components of the pathway were identified as tumor suppressor. The pathway was more concerned in fruit flies and mice, but little attention has been given in silkworm, an important economic and lepidopteran model insect. In this manuscript, we identified major Hippo pathway related genes(Hippo, Salvador, Warts, Mats, Yorkie) in silkworm and named BmHpo, BmSav, BmWts, BmMats, BmYki. The domain organization of these genes was highly conserved in silkworm and other organisms suggesting that they could use similar protein-protein interactions to construct the Hippo kinase cascades. The expression profiles of these genes in silkworm during embryonic, larval, wandering, pupal and adult stages were analyzed, 14 tissues/organs of the day 3, 5 th instar larvae(L5D3) as well. Experimental results showed that the expression of Hippo pathway had some influence on the development of silkworm. In order to find out the mechanism of Hippo pathway affecting silkworm development, BmHpo and BmYki were up-regulated and de-regulated in the cell line of Bombyx mori-BmN-SWU1(NS), and the changes of cell proliferation activity and cell cycle were detected. The distribution of BmYki was detected by immunofluorescence technique. This study provides insights into the genes of Hippo pathway which have a certain effect on somatic development and cell proliferation in silkworm.

关键词： Hippo pathway development cell proliferation cell cycle silkworm

RNAi of AbdB Gene in the Silkworm Embryo

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RNAi of AbdB Gene in the Silkworm Embryo

中国蚕学会第六届青年学术研讨会

作者： Chun-li Chai Xian-yan Wang Tian Tian fangyin dai Qingyou Xia Cheng Lu College of Biotechnology Southwest University Chongqing 400716 China

The products of the homeotic gene Abdominal-B play an essential role in determining the differentiation of abdominal posterior and terminal segments in insects. We have cloned a homologue of Abdominal-B from the silkworm Bombyx mori and studied its expression profile during embryonic development. Results showed that the silkworm Abdominal-B gene consists of at least two exons with a long 3’ UTR of 1907 bp,encoding for 319 amino acids. Its homeodomain sequence exhibits 100% identity with Drosophila melanogaster Abdominal-B gene. RT-PCR results revealed that its expression started around 30 h after egg laying,then increased gradually,reached the maximum at 7 d after egg laying. After that,its expression decreased once more and did not detected when the silkworm egg hatch. When the expression of Abdominal-B gene during silkworm embryonic development was silenced,the identities of segments from the tenth to the thirtieth（A7 to A10） were dramatically influenced. It causes excessive appendage sprout,caudal horn and valve mutate. The mutant profile is dose-dependent.

关键词： silkworm RNAi,embryonic development Abd-B

Near-field wideband beam training for ELAA with uniform circular array

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Science China(Information Sciences) 2024年第6期67卷 370-383页

作者： Yuhao CHEN Linglong dai Department of Electronic Engineering Tsinghua UniversityBeijing National Research Center for Information Science and Technology (BNRist)

Extremely large-scale antenna array(ELAA) at millimeter wave(mmWave) and Terahertz(THz)band has been considered a key technology for combating high attenuation in high-frequency bands in future6G communications. Uniform circular arrays(UCAs) have attracted much attention because of their ability to provide flat beamforming gain at all angles. To realize efficient beamforming, beam training is widely used to acquire channel state information. However, with a large antenna number, the beam training overhead in ELAA systems becomes overwhelming. Moreover, with a large bandwidth, the beam defocus effect severely degrades beam training accuracy. To address these issues, this paper proposes a frequency-dependent focusing(FDF)-based beam training scheme to realize effective beam training in near-field wideband ELAA systems with UCA. Specifically, we first analyze the FDF property of UCA, where signals at different subcarriers can simultaneously focus on different distances. Then, by exploiting the FDF property to search different distances using different subcarriers simultaneously, we design a hierarchical codebook and propose an FDFbased beam training scheme. To reveal the effectiveness of the proposed scheme, we compare its necessary beam training overhead with that of existing schemes. Finally, the simulation results demonstrate that the proposed scheme can achieve accurate beam training in near-field wideband UCA systems with a low beam training overhead.

关键词： extremely large-scale antenna array (ELAA) uniform circular array (UCA) near-field wideband beam training

Seismic performance evaluation of water supply pipes installed in a full-scale RC frame structure based on a shaking table test

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Earthquake Engineering and Engineering Vibration 2024年第1期23卷 163-178页

作者： Wu Houli Guo Endong Wang Jingyi dai Xin dai Chenxi Key Laboratory of Earthquake Engineering and Engineering Vibration Institute of Engineering MechanicsChina Earthquake AdministrationHarbin 150080China Key Laboratory of Earthquake Disaster Mitigation Ministry of Emergency ManagementHarbin 150080China

As an important part of nonstructural components,the seismic response of indoor water supply pipes deserves much *** paper presents shaking table test research on water supply pipes installed in a full-scale reinforced concrete(RC)frame *** material pipes and different methods for penetrating the reinforced concrete floors are combined to evaluate the difference in seismic *** response spectra and pipe acceleration amplification factors based on test data are discussed and compared with code provisions.A seismic fragility study of displacement demand is conducted based on numerical *** acceleration response and displacement response of different combinations are *** results show that the combination of different pipe materials and different passing-through methods can cause obvious differences in the seismic response of indoor riser pipes.

关键词： water supply pipe different materials shaking table test amplification factor seismic fragility

维普期刊数据库

Variation of lifespan in multiple strains,and effects of dietary restriction and BmFoxO on lifespan in silkworm,Bombyx mori

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Variation of lifespan in multiple strains,and effects of die...

中国蚕学会第九届青年学术研讨会

作者： Jiangbo Song Dongmei Tang Zhiquan Li Xiaoling Tong Jianfei Zhang Minjin Han Hai Hu Cheng Lu fangyin dai State Key Laboratory of Silkworm Genome Biology College of BiotechnologySouthwest University Key Laboratory for Sericulture Functional Genomics and Biotechnology of Agricultural Ministry College of BiotechnologySouthwest University

Established animal models have accelerated our understanding of the mechanisms involved in lifespan ***,more experimental animals are required to clarify the complex mechanisms behind the phenomena of aging and *** this study,we reported the variation of lifespan in nine distinct silkworm *** correlated significantly with Bm FoxO gene expression in the representative silkworm strains tested（Xiafang,Dazao-N,and N4）.In general,the female silkworm was longer lived than the male of the same *** restriction extended the silkworm lifespan compared with that of silkworms fed ad *** expression of Bm FoxO was significantly elevated in the dietary restriction group on day 3 of the 4 th instar and day 3 of the 5 th instar,suggesting that Bm FoxO contributes to dietary restriction-mediated lifespan *** RNA interference and overexpression of the Bm FoxO gene significantly shortened and extended the silkworm adulthood,*** conclusion,our findings suggest that the silkworm might serve as a promising experimental animal to explore the complex biological mechanisms of lifespan determination.

关键词： silkworm lifespan dietary restriction BmFoxO experimental animal Gerotarget

A serine protease homologue Bombyx mori scarface induces a short and fat body shape in silkworm

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A serine protease homologue Bombyx mori scarface induces a s...

中国蚕学会第十届青年学术研讨会

作者： Rixin Wang Xiaoling Tong Tingting Gai Chunlin Li Liang Qiao Hai Hu Minjin Han Zhonghuai Xiang Cheng Lu fangyin dai State Key Laboratory of Silkworm Genome Biology Key Laboratory of Sericultural Biology and Genetic BreedingMinistry of AgricultureSouthwest University Institute of Entomology and Molecular Biology College of Life SciencesChongqing Normal University

Body shape is one of the most prominent and basic characteristics of any *** insects,abundant variations in body shape can be observed both within and amongst ***,the molecular mechanism underlying body shape fine-tuning is very complex and has been largely unknown until *** the silkworm Bombyx mori,the tubby（tub） mutant has an abnormal short fat body shape and the abdomen of tub larvae expands to form a fusiform body *** investigation revealed that the body length was shorter and the body width was wider than that of the Dazao ***,this mutant is a good model for studying the molecular mechanisms of body shape *** positional cloning,we identified a gene encoding the serine protease homologue,*** scarface（Bmscarface）,which is associated with the tub *** analysis revealed a specific 312-bp deletion from an exon of Bmscarface in the tub *** addition,recombination was not observed between the tub and Bmscarface ***,RNA interference of Bmscarface resulted in the tub-like *** results indicate that Bmscarface is responsible for the tub mutant *** is the first study to report that mutation of a serine protease homologue can induce an abnormal body shape in insects.

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Defects in a serine protease homologue induce a short and fat body shape in the silkworm,Bombyx mori

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Defects in a serine protease homologue induce a short and fa...

中国蚕学会第九届青年学术研讨会

Body shape is one of the most prominent and basic characteristics of organisms and the underlying mechanisms are very complex and have largely unknown until *** insects,abundant variations in body shape between intra-species and inter-species can be *** far,the molecular mechanism of body shape fine-tuning is not well *** the silkworm,the tubby（tub） mutant has an abnormal short fat body shape and the abdomen of tub larvae expands to form a fusiform body ***,this mutant is a good model for studying the molecular mechanisms of body shape *** positional cloning,we identified a gene encoding the serine protease-like protein,Bmscarface,which is associated with the tub *** analysis revealed a specific 312-base-pair deletion from an exon of Bmscarface in the tub *** addition,recombination was not observed between the tub and Bmscarface ***,RNA interference of Bmscarface resulted in the tub-like *** results indicate that Bmscarface is responsible for the tub mutant *** is the first study to report that mutation of a serine protease homologue can induce an abnormal body shape in insects.

关键词： Bmscarface serine protease homologue body shape Silkworm