检索结果-南通市图书馆

Cell division cyclin 25C knockdown inhibits hepatocellular carcinoma development by inducing endoplasmic reticulum stress

引用

World Journal of Gastroenterology 2024年第19期30卷 2564-2574页

作者： Yan-Fei Li fang-yuan zheng Xin-Yu Miao Hai-Long Liu Yao-Yao Zhang Nai-Xia Chao Fa-Rong Mo School of Basic Medical Sciences Guangxi Medical UniversityNanning 530021Guangxi Zhuang Autonomous RegionChina Key Laboratory of Biological Molecular Medicine Research(Guangxi Medical University) Education Department of Guangxi Zhuang Autonomous RegionNanning 530021Guangxi Zhuang Autonomous RegionChina Key Laboratory of Human Development and Disease Research(Guangxi Medical University) Education Department of Guangxi Zhuang Autonomous RegionNanning 530021Guangxi Zhuang Autonomous RegionChina

BACKGROUND Cell division cyclin 25C(CDC25C)is a protein that plays a critical role in the cell cycle,specifically in the transition from the G2 phase to the M *** research has shown that CDC25C could be a potential therapeutic target for cancers,particularly for hepatocellular carcinoma(HCC).However,the specific regulatory mechanisms underlying the role of CDC25C in HCC tumorigenesis and development remain incompletely *** To explore the impact of CDC25C on cell proliferation and apoptosis,as well as its regulatory mechanisms in HCC *** Hepa1-6 and B16 cells were transduced with a lentiviral vector containing shRNA interference sequences(LV-CDC25C shRNA)to knock down ***,a xenograft mouse model was established by subcutaneously injecting transduced Hepa1-6 cells into C57BL/6 mice to assess the effects of CDC25C knockdown on HCC development in *** proliferation and migration were evaluated using a Cell Counting Kit-8 cell proliferation assays and wound healing assays,*** expression of endoplasmic reticulum(ER)stress-related molecules(glucose-regulated protein 78,X-box binding protein-1,and C/EBP homologous protein)was measured in both cells and subcutaneous xenografts using quantitative real-time PCR(qRT-PCR)and western ***,apoptosis was investigated using flow cytometry,qRT-PCR,and western *** CDC25C was stably suppressed in Hepa1-6 and B16 cells through LV-CDC25C shRNA transduction.A xenograft model with CDC25C knockdown was successfully established and that downregulation of CDC25C expression significantly inhibited HCC growth in ***25C knockdown not only inhibited cell proliferation and migration but also significantly increased the ER stress response,ultimately promoting ER stress-induced apoptosis in HCC *** The regulatory mechanism of CDC25C in HCC development may involve the activation of ER stress and the ER stress-induced apoptosis signali

关键词： Cell division cyclin 25C Hepatocellular carcinoma Endoplasmic reticulum stress Proliferation Apoptosis

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Is tumor necrosis factor-α monoclonal therapy with proactive therapeutic drug monitoring optimized for inflammatory bowel disease? Network meta-analysis

引用

World Journal of Gastrointestinal Surgery 2024年第2期16卷 571-584页

作者： fang-yuan zheng Kai-Si Yang Wen-Cheng Min Xin-Zhu Li Yu Xing Shuai Wang Ying-Shi Zhang Qing-Chun Zhao Teaching Hospital of Shenyang Pharmaceutical University General Hospital of Northern Theater CommandShenyang 110016Liaoning ProvinceChina

BACKGROUND The efficacy and safety of anti-tumor necrosis factor-α(TNF-α)monoclonal antibody therapy[adalimumab(ADA)and infliximab(IFX)]with therapeutic drug monitoring(TDM),which has been proposed for inflammatory bowel disease(IBD)patients,are still *** To determine the efficacy and safety of anti-TNF-αmonoclonal antibody therapy with proactive TDM in patients with IBD and to determine which subtype of IBD patients is most suitable for proactive TDM *** As of July 2023,we searched for randomized controlled trials(RCTs)and observa-tional studies in PubMed,Embase,and the Cochrane Library to compare anti-TNF-αmonoclonal antibody therapy with proactive TDM with therapy with reactive TDM or empiric *** and network meta-analyses were used to determine the IBD patient subtype that achieved clinical remission and to determine the need for *** This systematic review and meta-analysis yielded 13 studies after exclusion,and the baseline indicators were *** found a significant increase in the number of patients who achieved clinical remission in the ADA[odds ratio(OR)=1.416,95%confidence interval(CI):1.196-1.676]and RCT(OR=1.393,95%CI:1.182-1.641)subgroups and a significant decrease in the number of patients who needed surgery in the proactive vs reactive(OR=0.237,95%CI:0.101-0.558)and IFX+ADA(OR=0.137,95%CI:0.032-0.588)subgroups,and the overall risk of adverse events was reduced(OR=0.579,95%CI:0.391-0.858)according to the pairwise ***,the network meta-analysis results suggested that patients with IBD treated with ADA(OR=1.39,95%CI:1.19-1.63)were more likely to undergo TDM,especially in comparison with patients with reactive TDM(OR=1.38,95%CI:1.07-1.77).CONCLUSION Proactive TDM is more suitable for IBD patients treated with ADA and has obvious advantages over reactive *** recommend proactive TDM in IBD patients who are treated with ADA.

关键词： Inflammatory bowel disease Therapeutic drug monitoring Adalimumab Infliximab Network meta-analysis

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Network pharmacology investigation of the mechanism underlying the therapeutic action of Shikang granules in retinal ischemia-reperfusion injuries

引用

Integrative Medicine Discovery 2024年第17期8卷 1-8页

作者： Xiao-Xuan Wang Cong-Ying Wang Chi Zhang fang-yuan zheng Long-Hui Han Ming-Lian Zhang Hebei Eye Hospital Hebei Provincial Key Laboratory of OphthalmologyHebei Provincial Clinical Research Center for Eye DiseasesXingtai 054000China

Background:Retinal ischemia/reperfusion(I/R)injury often results in vision loss,and effective clinical management options are currently *** granules(SKG)are traditional Chinese medicine-based preparations commonly used in clinical practice for treating optic ***:Despite decades of clinical use,the precise mechanism of action(MoA)of SKG remains ***,we employ a network pharmacological approach to elucidate its MoA by identifying active ingredients and relevant targets using the Traditional Chinese Medicine System Pharmacology Database and Analytical *** associated with retinal I/R injury were sourced from GeneCards,Online Mendelian Inheritance in Man,and *** software facilitated the identification of intersecting targets,which were then subjected to gene ontology functional analysis and Kyoto Encyclopedia of Genes and Genomes pathway *** validate the protective effect and explore the MoA of SKG in retinal I/R injuries,we conducted experiments using rat ***:Our animal experiments demonstrated that SKG mitigated apoptosis following retinal I/R injury by upregulating the expression of the anti-apoptotic protein Bcl-2 and downregulating the expression of BAX,Caspase-9,Caspase-3,PARP,and cytochrome ***,SKG was found to increase the expression of PI3K and ***:SKG may exert its protective effects by inhibiting apoptosis through modulation of pro-apoptotic and anti-apoptotic protein expression,as well as activation of the PI3K/AKT pathway.

关键词： retinal ischemia-reperfusion injury Shikang granules apoptosis PI3K/AKT pathway

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Anti-hepatoma Effect of DC2.4 Cells Transfected with Tumor-Associated Antigen Cdc25C In Vitro

引用

Current Medical Science 2022年第3期42卷 491-497页

作者： Chun-mei LI Yan-fei LI Lin TIAN Qi-hui ZHANG fang-yuan zheng Fa-rong MO School of Basic Medical Science Guangxi Medical UniversityNanning 530021China Laboratory of Hepatobiliary and Pancreatic Surgery Affiliated Hospital of Guilin Medical UniversityGuilin 541001China Guangxi Colleges and Universities Key Laboratory of Human Development and Disease Research Guangxi Medical UniversityNanning 530021China

Objective Cell division cyclin 25 homolog C(Cdc25C)is a tumor-associated antigen candidate gene,and this may be used as an effective target in cancer *** present study aims to evaluate the lysis effect of cytotoxic T lymphocytes(CTLs)induced by dendritic cell line DC2.4 overexpressing Cdc25C,and the feasibility of Cdc25C as a component in hepatoma *** The mouse Cdc25C gene was ligated into a lentiviral vector,and transfected into DC2.4 *** DC2.4 cell phenotype and cytokine secretion were determined by flow cytometry and ELISA,***8^(+)T cells were sorted from the spleens of C57BL/6 mice using a magnetic bead sorting kit obtained from Miltenyi Biotech,Germany,and co-cultured with DC2.4 cells for one week as effector ***,IL-2,granzyme B and perforin were detected in the CTL culture medium by ***,time-resolved fluorescence immunoassay was used to detect the immune killing effect of Cdc25C-specific CTLs on target ***,the effect of blocking MHC-I sites on target cells with a monoclonal anti-MHC-I antibody was *** The results revealed that Cdc25C could be stably overexpressed in DC2.4 cells by LV-Cdc25C ***2.4 cells transfected with LV-Cdc25C secreted more IL-6,IL-12,TNF-αand IFN-γ,and had higher expression levels of CD40,CD86,CCR7 and MHC-II than unaltered DC2.4 *** elevated Cdc25C in dendritic cells also further increased the secretion of IL-2,granzyme B and perforin to elicit Cdc25C-specific CTLs,and induced the higher cytotoxicity in Hepa1-6 cell lines(P<0.05),but this had no effect on the target cells when MHC-I monoclonal antibodies were *** DC2.4 cells transfected with LV-Cdc25C can induce specific CTLs,and result in a strong cellular immune *** dendritic cells that overexpress Cdc25C may be useful for hepatoma immunotherapy.

关键词： dendritic cells cell division cyclin 25 homolog C cytotoxic T lymphocytes hepatocellular carcinoma anti-hepatoma

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Temperature-responsive Behavior of Polymer Fluorescent System via Electrostatic Interaction Mediated Aggregation/Deaggregation

引用

Chinese Journal of Polymer Science 2016年第7期34卷 830-837页

作者： si-hua guo fang-yuan zheng fang zeng 吴水珠 College of Materials Science and Engineering State Key Laboratory of Luminescent Materials and Devices South China University of Technology Guangzhou 510640 China

A simple and effective polymer fluorescent thermosensitive system was successfully developed based on the synergistic effect of excimer/monomer interconversion of pyrene derivatives and electrostatic interaction between polyelectrolyte and charged fluorophore. As for the system, the excimer-monomer conversion, thermosensitive behavior and thermo-responsive reversibility were investigated experimentally. Temperature variation and temperature-distribution induced fluorescence changes can be observed directly by naked eyes. Thus, this polymer system holds promise for serving as a fluorescent thermometer.

关键词： Polymer fluorescent thermometer Temperature-responsive Electrostatic interaction Aggregation Deaggregation

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

新烟碱类农药残留检测中基质效应的影响及校正

实验室检测

引用

实验室检测 2024年第1期2卷 117-122页

作者：史海传郜效儒袁方正朱振隗玉高艾英泰安市食品药品检验检测研究院(泰安市纤维检验所) 泰安271000

目的探究基质效应对生姜、香蕉中吡虫啉、噻虫嗪、噻虫胺定量分析结果的影响和影响基质效应的因素,并提出补偿基质效应的方法。方法采用QuEChERS法前处理,用液相色谱-串联质谱法测定并计算基质效应,通过溶剂标、基质标分别校正样品和质... 详细信息

目的探究基质效应对生姜、香蕉中吡虫啉、噻虫嗪、噻虫胺定量分析结果的影响和影响基质效应的因素,并提出补偿基质效应的方法。方法采用QuEChERS法前处理,用液相色谱-串联质谱法测定并计算基质效应,通过溶剂标、基质标分别校正样品和质控验证基质标校正可行性。结果基质标校正的标准曲线的线性均在0.999以上;测得样品结果的精密度优于溶剂标,相对标准偏差在2.1%~8.3%之间;测得质控样品的回收率在86.7%~103.0%之间。基质效应会影响定量分析的标准曲线线性、精密度和准确度,同时基质效应会受到基质浓度、基质种类、基质个体差异和农药结构的影响。基质标校正的标准曲线线性、精密度、准确度明显优于溶剂标,采用基质标校正可有效补偿定量分析中基质效应对准确度和精密度的影响。结论本研究结果为新烟碱类化合物实际分析检测中基质效应的评估提供了依据,为得到更为精准的定量结果提供了方向。

关键词：新烟碱类杀虫剂农药残留检测基质效应液相色谱-串联质谱法

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Dynamic recrystallization behavior and strengthening mechanism of a novel Mo-Ti3AlC2 alloy at ultrahigh temperature

引用

Rare Metals 2024年第8期43卷 3946-3960页

作者： Lu Yang Xin-yuan zheng Yang Zhao Xi-Ran Wang fang-Nao Xiao Shi-Zhong Wei School of Materials Science and Engineering Henan University of Science and Technology National Joint Engineering Research Center for Abrasion Control and Molding of Metal Materials Henan University of Science and Technology Department of Applied Mechanics Univ.Bourgogne FrancheComteFEMTO-ST InstituteCNRS/UF/ENSMM/UTBM

Increasing the recrystallization temperature to achieve better high-temperature performance is critical in the development of molybdenum alloys for ultrahightemperature applications,such as the newest generation of multitype high-temperature nuclear *** this study,an innovative strategy was proposed to improve the performance of molybdenum alloys at high temperature by using the two-dimensional MAX（where M is an early transition metal,A is an A-group element and X is C or N）ceramic material *** relationships between flow stress,strain rate and temperature were *** microstructure,distribution of misorientation and evolution of dislocations in the Mo-Ti3AlC2alloy were *** microscopic mechanism of the Ti3AlC2phase in the molybdenum alloy at high temperatures was *** experimental results showed that the peak flow stress of Mo-Ti3AlC2at 1600℃ reached 155 MPa,which was161.8% greater than that of pure *** activation energy of thermal deformation of Mo-Ti3AlC2was as large as537 kJ·mol-1,which was 17.6% more than that of pure *** recrystallization temperature reached 1600℃ or even *** topological reaction of the Ti3AlC2phase consumed a large amount of energy at high temperatures,resulting in increases in the deformation activation *** structures of AlTi3and Ti-O Magneli-phase oxides(Ti_nO2n-1) were formed in-situ,which relied on kink bands and interlayer slip,resulting in many dislocations during ***,the special two-dimensional of the structure Ti3AlC2ceramic inhibited the recrystallization behavior of the Mo *** results of this study can provide theoretical guidance for the development of a new generation of molybdenum alloys for use in ultrahigh-temperature environments.

关键词： Mo alloy Two-dimensional reinforcement Dynamic recrystallization behavior Hot deformation Mechanical properties

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

A Trust Evaluation Mechanism Based on Autoencoder Clustering Algorithm for Edge Device Access of IoT

引用

Computers, Materials & Continua 2024年第2期78卷 1881-1895页

作者： Xiao Feng zheng yuan Cyber Security Academy Beijing University of Posts and TelecommunicationsBeijing100876China Department of Cyberspace Security Beijing Electronic Science and Technology InstituteBeijing100070China State Grid Info-Telecom Great Power Science and Technology Co. Ltd.Beijing102211China

First,we propose a cross-domain authentication architecture based on trust evaluation mechanism,including registration,certificate issuance,and cross-domain authentication processes.A direct trust evaluation mechanism based on the time decay factor is proposed,taking into account the influence of historical interaction *** weight the time attenuation factor to each historical interaction record for updating and got the new historical record *** refer to the beta distribution to enhance the flexibility and adaptability of the direct trust assessment model to better capture time trends in the historical *** we propose an autoencoder-based trust clustering *** perform feature extraction based on *** leibler(KL)divergence is used to calculate the reconstruction *** constructing a convolutional autoencoder,we introduce convolutional neural networks to improve training efficiency and introduce sparse constraints into the hidden layer of the *** sparse penalty term in the loss function measures the difference through the KL *** clustering is performed based on the density based spatial clustering of applications with noise(DBSCAN)clustering *** the clustering process,edge nodes have a variety of trustworthy attribute *** assign different attribute weights according to the relative importance of each attribute in the clustering process,and a larger weight means that the attribute occupies a greater weight in the calculation of ***,we introduced adaptive weights to calculate comprehensive trust *** experiments prove that our trust evaluation mechanism has excellent reliability and accuracy.

关键词： Cross-domain authentication trust evaluation autoencoder

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Advance in reversible Zn anodes promoted by 2D materials

引用

Rare Metals 2024年第4期43卷 1350-1369页

作者： Shu-yuan Lei Jin-Xiu Feng Yu-Chao Chen Dong zheng Wen-Xian Liu Wen-Hui Shi fang-fang Wu Xie-Hong Cao School of Materials Science and Engineering Zhejiang University of TechnologyHangzhou310014China Center for Membrane Separation and Water Science and Technology College of Chemical EngineeringZhejiang University of TechnologyHangzhou310014China

With the growing energy demand associated with high safety and low-cost requirement,aqueous zinc-ion batteries(AZIBs)have been considered as one of the most promising next-generation ***,some key issues,such as uncontrollable dendrites growth,severe corrosion,hydrogen evolution and side reactions of Zn anodes during charge/discharge process,have hindered its pragmatic ***-dimensional(2D)materials hold advantages of unique physical and chemical properties,large surface areas and abundant active sites,which have been successfully used to overcome the above shortcomings of Zn anodes in recent *** this review,the issues and challenges of Zn anodes are ***,the state-of-the-art progress on Zn anodes modification based on 2D materials such as graphene,2D metal carbides and nitrides(MXenes),2D metal-organic frameworks(MOFs),2D covalent organic frameworks(COFs),2D transition metal compounds and other 2D materials is discussed in ***,the perspectives of employing 2D materials in highly reversible Zn anodes are summarized and discussed.

关键词： 2D materials Aqueous zinc-ion battery Zn anodes Dendrites growth

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Data-driven discovery of linear dynamical systems from noisy data

引用

Science China(Technological Sciences) 2024年第1期67卷 121-129页

作者： WANG YaSen yuan Ye fang HuaZhen DING Han School of Mechanical Science and Engineering Huazhong University of Science and TechnologyWuhan 430074China State Key Lab of Digital Manufacturing Equipment and Technology Huazhong University of Science and TechnologyWuhan 430074China School of Artificial Intelligence and Automation Huazhong University of Science and TechnologyWuhan 430074China Department of Mechanical Engineering University of KansasLawrence 66045USA

In modern science and engineering disciplines,data-driven discovery methods play a fundamental role in system modeling,as data serve as the external representations of the intrinsic mechanisms within ***,empirical data contaminated by process and measurement noise remain a significant obstacle for this type of *** this study,we have developed a data-driven method capable of directly uncovering linear dynamical systems from noisy *** method combines the Kalman smoothing and sparse Bayesian learning to decouple process and measurement noise under the expectation-maximization framework,presenting an analytical method for alternate state estimation and system ***,the discovered model explicitly characterizes the probability distribution of process and measurement noise,as they are essential for filtering,smoothing,and stochastic *** have successfully applied the proposed algorithm to several simulation *** results demonstrate its potential to enable linear dynamical system discovery in practical applications where noise-free data are intractable to capture.

关键词： system identification sparse Bayesian learning Kalman smoothing process and measurement noise

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：