Network pharmacology investigation of the mechanism underlying the therapeutic action of Shikang granules in retinal ischemia-reperfusion injuries
作者机构：Hebei Eye HospitalHebei Provincial Key Laboratory of OphthalmologyHebei Provincial Clinical Research Center for Eye DiseasesXingtai 054000China
出 版 物：《Integrative Medicine Discovery》
年 卷 期：2024年第8卷第17期
页 面：1-8页
学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学]
基 金：supported by the S&T Program of Xingtai(2023ZC178)
主 题：retinal ischemia-reperfusion injury Shikang granules apoptosis PI3K/AKT pathway
摘 要：Background:Retinal ischemia/reperfusion(I/R)injury often results in vision loss,and effective clinical management options are currently *** granules(SKG)are traditional Chinese medicine-based preparations commonly used in clinical practice for treating optic ***:Despite decades of clinical use,the precise mechanism of action(MoA)of SKG remains ***,we employ a network pharmacological approach to elucidate its MoA by identifying active ingredients and relevant targets using the Traditional Chinese Medicine System Pharmacology Database and Analytical *** associated with retinal I/R injury were sourced from GeneCards,Online Mendelian Inheritance in Man,and *** software facilitated the identification of intersecting targets,which were then subjected to gene ontology functional analysis and Kyoto Encyclopedia of Genes and Genomes pathway *** validate the protective effect and explore the MoA of SKG in retinal I/R injuries,we conducted experiments using rat ***:Our animal experiments demonstrated that SKG mitigated apoptosis following retinal I/R injury by upregulating the expression of the anti-apoptotic protein Bcl-2 and downregulating the expression of BAX,Caspase-9,Caspase-3,PARP,and cytochrome ***,SKG was found to increase the expression of PI3K and ***:SKG may exert its protective effects by inhibiting apoptosis through modulation of pro-apoptotic and anti-apoptotic protein expression,as well as activation of the PI3K/AKT pathway.
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