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Biodegradation of Phthalate Esters by a Newly Isolated Acinetobacter sp. Strain LMB-5 and Characteristics of Its Esterase

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Pedosphere 2017年第3期27卷 606-615页

作者： fang yue ZHANG Lishuang wang Jing ZHOU Ying YE Bangce Lab of Biosystems and Microanalysis State Key Laboratory of Bioreactor Engineering East China University of Science and Technology School of Chemistry and Chemical Engineering Shihezi University

Phthalate esters （PAEs） are extensively applied in industry, and they migrate to environment during the process of production, employ, and treatment and axe difficult to be degraded in nature. However, some microorganisms could use them as the carbon source to growth. In this study, an Acinetobacter sp. strain LMB-5, capable of utilizing PAEs, was isolated from a vegetable greenhouse soil. The degradation capability of strain LMB-5 was also investigated by incubation in mineral salt medium containing different PAEs, dimethyl phthalate （DMP）, diethyl phthalate （DEP）, di-n-butyl phthalate （DBP）, and di-（2-ethylhexyl） phthalate （DEHP）. The strain could grow well with DMP, DEP, DBP, and DEHP. When the concentration of DBP increased from 100 to 400 mg L-1, the half-life extended from 9.5 to 15.5 h. In the concentration range of DBP, the degradation ability of strain LMB-5 could be described by first-order kinetics. During the biodegradation of DBP, three intermediates, 1,2-benzenedicaxboxylic acid,butyl methyl ester, DMP, and phthalic acid （PA） were detected, and the proposed pathway of DBP was identified. By analysis of bioinformatics, one esterase was cloned from the genome of LMB-5 and expressed in Escherichia coll. It displayed an ability to break the ester bonds of DBP. The enzyme exhibited maximal activity at pH 7.0 and 40 ℃ with DBP as the substrate. It was activated by Cu2＋ and Fe3＋ and had a high activity in the presence of low concentrations of methanol or dimethylsulfoxide （each 10%, volume：volume）. The Acinetobacter sp. strain LMB-5 may make a contribution to the remediation of soils polluted by PAEs in the future.

关键词： biological toxicity degradation bacteria di-n-butyl phthalate enzymatic activity hydrolase soil pollution

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Mean sea surface heights of the South and East China Seas from ocean circulation model and geodetic leveling

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Chinese Science Bulletin 2002年第4期47卷 326-329页

作者： fang Guohong WEI Zexun fang yue wang Kai Choi Byungho Institute of Oceanology Chinese Academy of SciencesQingdao 266071China Department of Oceanography Texas A&M UniversityCollege StationTX 77843-3146USA Department of Civil Engineering Sungkyunkwan UniversitySuwon440-746Korea

The mean sea surface heights (sea surface topography) of the South China, East China, Yellow and Bohai Seas are derived from an ocean general circulation model and surface air pressure. The circulation model covers the global oceans, with fine grid (1/6°)? covering the East Asian marginal seas and coarse grid (3°) covering the rest part of the global oceans. The result shows that the China 1985 Na-tional Altitude Datum is 24.7 cm above the mean sea surface height of the world oceans. The mean sea surface in the coastal ocean adjacent to China is higher in the south than in the north. Intercomparison of the model results with the geodetic leveling measurements at 28 coastal tidal stations shows a standard deviation of 4.8 cm and a fitting coefficient of 95.3%. After correction through linear regression, the standard deviation is reduced to 4.5 cm. This indicates that the accuracy of model results is sufficient for practical application. Based on the model results, the mean sea surface heights for the

关键词： mean sea surface height ocean circulation model land leveling South China Sea East China Sea Yellow Sea Bohai Sea.

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Synergistically enhanced ORR and HER performance on Co-N-C coupled in-situ generated PtCo intermetallic

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Journal of Energy Chemistry 2025年第1期100卷 721-729页

作者： Guanyu Luo Min Song Lulu An Xiao Huang Qian Zhang Chenhao Zhang Tao Shen Shuang wang Deli wang Key Laboratory of Material Chemistry for Energy Conversion and Storage (Huazhong University of Science and Technology) Ministry of Education School of Chemistry and Chemical Engineering Huazhong University of Science and Technology

Integrating multi-scale sites in a composite catalyst is vital to realize efficient ***,a synergistic composite catalyst consisting of Co atomic sites and in-situ generated PtCo intermetallic compounds （IMCs）(o-PtCo@CoNC) is proposed through Co pre-anchoring and subsequent impregnation-reduction *** loading of Co atoms provides a chance for in-situ generating PtCo ordered intermetallic *** remaining Co single atoms and PtCo IMCs construct synergistic electrocatalytic *** from the ordered structure,synergistic effect of Pt Co IMCs and Co single atoms,o-PtCo@CoNC exhibits excellent electrocatalytic performance for oxygen reduction reaction （ORR） and hydrogen evolution reaction （HER） with mass activity of 1.21 A mgPt-1（at 0.9 V） and5.70 A mgPt-1（at an overpotential of 100 mV）,***,o-PtCo@CoNC delivers negligible loss of half-wave potential and overpotential during long-term stability test in acid solutions,with 13 mV decay after 50,000 potential cycles for ORR and a 2.7 mV decay after 20,000 potential cycles for *** integration strategy of single-atomic sites coupled IMCs paves the way for enhancing the activity and durability of Pt-based electrocatalysts.

关键词： Electrolytic reduction

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EZH2-dependent myelination following sciatic nerve injury

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Neural Regeneration Research 2025年第8期20卷 2382-2394页

作者： Hui Zhu Li Mu Xi Xu Tianyi Huang Ying wang Siyuan Xu Yiting wang Wencong wang Zhiping wang Hongkui wang Chengbin Xue Research Center of Clinical Medicine Affiliated Hospital of Nantong UniversityKey Laboratory of Neuroregeneration of Jiangsu and Ministry of EducationCo-innovation Center of NeuroregenerationNMPA Key Laboratory for Research and Evaluation of Tissue Engineering Technology ProductsNantong UniversityNantongJiangsu ProvinceChina Medical School of Nantong University NantongJiangsu ProvinceChina Department of Neurology Affiliated Hospital of Nantong UniversityNantongJiangsu ProvinceChina Department of Rehabilitation Medicine Affiliated Hospital of Nantong UniversityNantongJiangsu ProvinceChina Department of Critical Care Medicine Nantong Fourth People’s HospitalNantongJiangsu ProvinceChina

Demyelination and remyelination have been major focal points in the study of peripheral nerve regeneration following peripheral nerve ***,the gene regulatory network of regenerated myelin differs from that of native *** of enhancer of zeste homolog 2(EZH2)hinders the differentiation,maturation,and myelination of Schwann cells in *** further determine the role of EZH2 in myelination and recovery post-peripheral nerve injury,conditional knockout mice lacking Ezh2 in Schwann cells(Ezh2^(fl/fl);Dhh-Cre and Ezh2^(fl/fl);Mpz-Cre)were *** results show that a significant proportion of axons in the sciatic nerve of Ezh2-depleted mice remain *** highlights the crucial role of Ezh2 in initiating Schwann cell ***,we observed that 21 days after inducing a sciatic nerve crush injury in these mice,most axons had remyelinated at the injury site in the control nerve,while Ezh2^(fl/fl);Mpz-Cre mice had significantly fewer remyelinated axons compared with their wild-type *** suggests that the absence of Ezh2 in Schwann cells impairs myelin formation and *** conclusion,EZH2 has emerged as a pivotal regulatory factor in the process of demyelination and myelin regeneration following peripheral nerve *** EZH2 activity during these processes may offer a promising therapeutic target for the treatment of peripheral nerve injuries.

关键词： demyelination EZH2 myelination peripheral nerve injury PRC2 remyelination Schwann cells sciatic nerve crush sciatic nerve transection

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控制方向未知的受限多智能体系统的预设时间模糊控制

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工程科学学报 2025年第1期47卷 121-129页

作者：李菲周超范利蓉王芳燕山大学理学院秦皇岛066004 河北农业大学海洋学院秦皇岛066003

综合考虑受控制方向未知、输入受限和状态时延影响的有领导者多智能体系统的编队控制问题,设计基于模糊逻辑系统的预设时间一致性控制策略.为了保证编队输出误差在预设时间内满足预定的约束范围要求,引入预设时间性能函数,构造Lyapunov-... 详细信息

综合考虑受控制方向未知、输入受限和状态时延影响的有领导者多智能体系统的编队控制问题,设计基于模糊逻辑系统的预设时间一致性控制策略.为了保证编队输出误差在预设时间内满足预定的约束范围要求,引入预设时间性能函数,构造Lyapunov-Krasovskii(L-K)泛函解决状态时延问题,将外界干扰和L-K泛函的导数中的部分项定义为未知非线性函数,并利用模糊逻辑系统对其进行估计,利用Nussbaum函数和均值定理分别处理控制方向未知和输入受限问题,基于以上设计,提出预设时间模糊控制策略,并通过Lyapunov稳定性理论,分析闭环系统的有界稳定性,数值对比仿真和两级化学反应器应用仿真说明控制方法的有效性.

关键词：多智能体系统状态时延输出误差约束输入受限控制方向未知

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Microbial community changes in the digestive tract of the clam Meretrix petechialis in response to Vibrio parahaemolyticus challenge

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Journal of Oceanology and Limnology 2021年第1期39卷 329-339页

作者： Hongxia wang Xin yue Jiajia YU Rui wang Shuangshuang TENG Jun fang Baozhong LIU CAS Key Laboratory of Experimental Marine Biology Institute of OceanologyCenter for Ocean Mega-ScienceChinese Academy of SciencesQingdao 266071China Laboratory for Marine Biology and Biotechnology Qingdao National Laboratory for Marine Science and TechnologyQingdao 266000China Zhejiang Mariculture Research Institute Wenzhou 325005China Zhejiang Key Laboratory of Exploitation and Preservation of Coastal Bio-resource Wenzhou 325005China

Disease in clams frequently occurred over the last decade and has become a serious threat to the clam aquaculture industry and natural *** clam mortality events were reported to be associated with the presence of opportunistic pathogen ***,the complexity of infection that occurs in the natural environment remains poorly *** this study,we smulated a natural disease outbreak by vibrio immersion infection to study the diversity and dynamics of microbiota in the digestive tract of clam Meretrix petechialis during the infection *** changes in operational taxonomic unit richness and phylum composition of the bacterial communities were observed during pathogen *** addition,we investigated the potential relationship between microbiota dynamics and host status during disease *** reveal that,at the end stage of vibrio infection,interindividual variation in the digestive tract microbiota increased,as did the diff erence in individual health *** moribund clams displayed signs of microbial community shifts to low diversity,and the microbial community was characterized by mass proliferation of a few operational taxonomic units.

关键词： Meretrix petechialis vibrio challenge 16S ribosomal RNA bacterial community digestive tract

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Molecular Dynamics Simulations of the Orientation Effect on the Initial Plastic Deformation of Magnesium Single Crystals

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Acta Metallurgica Sinica(English Letters) 2016年第3期29卷 301-312页

作者： Qun Zu Ya-fang Guo Shuang Xu Xiao-Zhi Tang yue-Sheng wang Institute of Engineering Mechanics Beijing Jiaotong University School of Science Wuhan University of Technology

Molecular dynamics simulation is employed to study the tension and compression deformation behaviors of magnesium single crystals with different *** angle between the loading axis and the basal direction ranges from 0° to 90°.The simulation results show that the initial defects usually nucleate at free surfaces,but the initial plastic deformation and the subsequent microstructural evolutions are various due to different loading *** tension simulations exhibit the deformation mechanisms of twinning,slip,crystallographic reorientation and basal/prismatic *** twinning,crystallographic reorientation and basal/prismatic transformation can only appear in the crystal model loaded along or near the a-axis or *** the compression simulations,the basal,prismatic and pyramidal slips are responsible for the initial plasticity,and no twinning is ***,the plastic deformation models affect the yield strengths for the samples with different *** maximum yield stresses for the samples loaded along the c-axis or a-axis are much higher than those loaded in other directions.

关键词： Molecular dynamics simulations Magnesium Plastic deformation Orientation

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Satellite remote sensing reveals overwhelming recovery of forest from disturbances in Asia

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Atmospheric and Oceanic Science Letters 2025年第1期18卷 46-51页

作者： Yiying Zhu Hesong wang Anzhi Zhang School of Ecology and Nature Conservation Beijing Forestry UniversityBeijingChina Key Laboratory of Regional Climate and Environment for Temperate East Asia Institute of Atmospheric PhysicsChinese Academy of SciencesBeijingChina College of Forestry and Landscape Architecture Xinjiang Agricultural UniversityUrumqiChina

Forest ecosystems play key roles in mitigating human-induced climate change through enhanced carbon uptake;however,frequently occurring climate extremes and human activities have considerably threatened the stability of *** the same time,detailed accounts of disturbances and forest responses are not yet well quantified in *** study employed the Breaks For Additive Seasonal and Trend method-an abrupt-change detection method-to analyze the Enhanced Vegetation Index time series in East Asia,South Asia,and Southeast *** approach allowed us to detect forest disturbance and quantify the resilience after *** showed that 20%of forests experienced disturbance with an increasing trend from 2000 to 2022,and Southeast Asian countries were more severely affected by ***,95%of forests had robust resilience and could recover from disturbance within a few *** resilience of forests suffering from greater magnitude of disturbance tended to be stronger than forests with lower disturbance *** summary,this study investigated the resilience of forests across the low and middle latitudes of Asia over the past two *** authors found that most forests exhibited good resilience after disturbance and about two-thirds had recovered to a better state in *** findings of this study underscore the complex relationship between disturbance and resilience,contributing to comprehension of forest resilience through satellite remote sensing.

关键词： Forest ecosystem Enhanced vegetation index Breaks for additive seasonal and trend method Disturbance Resilience

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套式PCR检测体系扩增效率的分析及对假阳性的控制

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微生物学免疫学进展 2001年第1期29卷 43-48页

作者：方悦群王春晖谢贵林王秉瑞兰州生物制品研究所兰州730046

用扩增效率等量化的指标来控制PCR检测过程中出现的假阳性 ,调查了受假阳性污染的试剂在特定的PCR检测系统和环境中产生假阳性的PCR循环数界限 ,采用模板量与扩增倍数的线性回归关系分析系统的扩增效率并以此测定计算了两个不同循环数系... 详细信息

用扩增效率等量化的指标来控制PCR检测过程中出现的假阳性 ,调查了受假阳性污染的试剂在特定的PCR检测系统和环境中产生假阳性的PCR循环数界限 ,采用模板量与扩增倍数的线性回归关系分析系统的扩增效率并以此测定计算了两个不同循环数系统 (C2 7+15) 和C2 7+2 0 )的检测界限分别为 0 78fg和 0 0 6 5fg ,提出以此检测界限的 1/2量作为假阳性耐受量 ,两个不同循环数系统的假阳性耐受量分别为 5 80个和 48个拷贝。

关键词：多聚酶链反应假阳性质量控制 PCR检测

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Microstructure analysis of AISI 304 stainless steel produced by twin-roll thin strip casting process

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Journal of University of Science and Technology Beijing 2007年第5期14卷 420-424页

作者： Zhenmin wang Yuan fang Junjie Qi yue Zhang Yan Yu Jianchun Wu State Key Laboratory for Advanced Metals and Matefiai University of Science and Technology Beijing Beijing 100083 China R＆D Center Baosteel Advanced Technology Institute Shanghai 201900 China School of Material Science and Engineering University of Science and Technology Liaoning Anshan 114044 China

The microstructure of AISI 304 austenlte stainless steel fabricated by the thin strip casting process were investigated using optical microscope, scanning electron microscope （SEM）, transmission electron microscope （TEM）, and X-ray diffraction （XRD）. The microstructures of the casting strips show a duplex structure consisting of delta ferrite and austenite. The volume fraction of the delta ferrite is about 9.74vo1% at the center and 6.77vo1% at the surface of the casting thin strip, in vermicular and hand shapes. On account of rapid cooling and solidification in the continuous casting process, many kinds of inclusions and precipitates have been found. Most of the inclusions and precipitates are spherical complex compounds consisting of oxides, such as, SiO2, MnO, Al2O3, Cr2O3, and FeO or their multiplicity oxides of ***2O3, ***2, and ***2. Many defects including dislocations and stacking faults have also formed during the rapid cooling and solidification process, which is helpful to improve the mechanical properties of the casting strips.

关键词： austenite stainless steel casting thin strip morphology microstructure

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