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The protoplast two-way fusions and fusant characteristics of Antrodia cinnamomea and Cordyceps militaris

Food Science and Human Wellness 2022年第5期11卷 1240-1251页

作者： Wei-Kuang Lai Yang-Chang Wu Tai-Sheng Yeh Chun-Ren Hsieh Yi-Hong Tsai Chien-Kei Wei Chi-Ying Li Ying-Chen lu fang-Rong Chang Graduate Institute of Natural Products Kaohsiung Medical UniversityKaohsiung 807TaiwanChina Graduate Institute of Integrated Medicine China Medical UniversityTaichung 404TaiwanChina Chinese Medicine Research and Development Center China Medical University HospitalTaichung 404TaiwanChina Department of Food Science and Nutrition Meiho UniversityPingtung 912TaiwanChina Department of Food Science National Chiayi UniversityChiayi City 600TaiwanChina Drug Development and Value Creation Research Center Kaohsiung Medical UniversityKaohsiung 807TaiwanChina Department of Medical Research Kaohsiung Medical University HospitalKaohsiung Medical UniversityKaohsiung 807TaiwanChina Department of Marine Biotechnology and Resources National Sun Yat-sen UniversityKaohsiung 804TaiwanChina

This study generated two fused protoplasts of Antrodia cinnamomea and Cordyceps militaris in two *** protoplasts of *** were inactivated by heat to inactivate biochemical processes and enzymatic activities in the cytoplasm,and the protoplasts of *** were inactivated by UV radiation to invalidate their genome function,then they were fused under optimal conditions to get a fusion rate as(7.42±0.8)×10^(-6) fusants/mL;the new fusants were abbreviated as *** the other hand,when *** and *** were treated with heat and UV oppositely using similar experiments,the fusion rate was(9.70±0.68)×10^(-5) fusants/mL,and the new fusants were abbreviated as *** selected each of two best-growing fused colonies Ac-Cm-1,Ac-Cm-2,Cm-Ac-1,and Cm-Ac-2,together with parental *** and ***,and studied their morphology,growth antagonism tests,and genetic relationships by 18 S rRNA *** comparison with the initial cultures of 4 fusants,the yields of adenosine,biomass,cordycepic acid,cordycepin,total polysaccharide,and total triterpenoids were increased up 1.305-50.1563 times in the optimal medium *** gene stability tests,those of the four fusants and their outputs were stabilized within 10 generations.

关键词： Medicinal fungi Protoplast fusion Antrodia cinnamomea Cordyceps militaris Secondary metabolite

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A critical review of biochar-based nitrogen fertilizers and their effects on crop production and the environment

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Biochar 2022年第1期4卷 577-595页

作者： Yurong Gao Zheng fang lukas Van Zwieten Nanthi Bolan Da Dong Bert F.Quin jun Meng fangbai Li Fengchang Wu Hailong Wang Wenfu Chen Agronomy College Shenyang Agricultural UniversityShenyang 110866China Biochar Engineering Technology Research Center of Guangdong Province School of Environmental and Chemical EngineeringFoshan UniversityFoshan 528000China Key Laboratory of Biochar and Soil Improvement Ministry of Agriculture and Rural AffairsBeijingChina Department of Primary Industries New South Wales1243 Bruxner Highway2477 WollongbarNSWAustralia School of Agriculture and Environment The University of Western AustraliaPerthWA 6001Australia The UWA Institute of Agriculture The University of Western Australia6001 PerthWAAustralia School of Environmental and Resource Sciences Zhejiang A&F UniversityHangzhouZhejiang 311300China Quin Environmentals(NZ)Ltd PO Box 125122St.HeliersAuckland 1740New Zealand Guangdong Key Laboratory of Integrated Agro-Environmental Pollution Control and Management Institute of Eco-environmental and Soil SciencesGuangdong Academy of Sciences510650 GuangzhouChina State Key Laboratory of Environmental Criteria and Risk Assessment Chinese Research Academy of Environmental SciencesBeijing 100012China Guangdong Green Technologies Co. LtdFoshan 528100China

Globally,nitrogen(N)fertilizer demand is expected to reach 112 million tonnes to support food production for about 8 billion ***,more than half of the N fertilizer is lost to the environment with impacts on air,water and soil quality,and ***,N loss to the environment contributes to greenhouse gas emissions and climate ***,where N fertilizer application is limited,severe depletion of soil fertility has become a major constraint to sustainable *** address the issues of low fertilizer N use efficiency(NUE),biochar-based N fertilizers(BBNFs)have been developed to reduce off-site loss and maximize crop N *** products are generally made through physical mixing of biochar and N fertilizer or via coating chemical N fertilizers such as prilled urea with *** review aims to describe the manufacturing processes of BBNFs,and to critically assess the effects of the products on soil properties,crop yield and N loss pathways.

关键词： Nitrogen use efficiency Controlled-release Retention Coating Biochar removing contaminants from

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Identification of a delayed leaf greening gene from a mutation of pummelo

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Science China(Life Sciences) 2021年第7期64卷 1165-1173页

作者： Hui-Wen Yu Zhi-Hao lu Xia Wang Dan Liu Jia-Xian He Xiao-Lin Jiang Ling-jun Ke Wen-Wu Guo Xiu-Xin Deng Qiang Xu Key Laboratory of Horticultural Plant Biology(Ministry of Education) Huazhong Agricultural UniversityWuhan 430070China Key Laboratory of Landscape Plants with Fujian and Taiwan Characteristics ofFujian Colleges and Universities Minnan Normal UniversityZhangzhou 363000China

Delayed greening of young leaves is an unusual phenomenon of plants in *** are mostly evergreen tree ***,a natural mutant of“Guanxi”pummelo(Citrus maxima),which shows yellow leaves at the young stage,was characterized to identify the genes underlying the trait of delayed leaf greening in plants.A segregating population with this mutant as the seed parent and a normal genotype as the pollen parent was *** DNA pools respectively from the leaves of segregating seedlings with extreme phenotypes of normal leaf greening and delayed leaf greening were collected for *** segregant analysis(BSA)and In Del marker analysis demonstrated that the delayed leaf greening trait is governed by a 0.3 Mb candidate region on chromosome *** expression analysis further identified a key candidate gene(Citrus Delayed Greening gene 1,CDG1)in the 0.3 Mb region,which showed significantly differential expression between the genotypes with delayed and normal leaf greening *** was a 67 bp In Del region difference in the CDG1 promoter and the In Del region contains a TATA-box *** laser-scanning microscopy revealed that the CDG1-GFP fusion protein signals were co-localized with the chloroplast signals in the *** of CDG1 in tobacco and Arabidopsis led to the phenotype of delayed leaf *** results suggest that the CDG1 gene is involved in controlling the delayed leaf greening phenotype with important functions in chloroplast development.

关键词： citrus pummelo delayed leaf greening chlorophyll

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自动小车存取系统复合作业三维空间路径优化

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计算机集成制造系统 2015年第3期21卷 702-708页

作者：方彦军唐猛武汉大学自动化系湖北武汉430072

为了提高智能立体仓库的运转效率,提出了一种改进的人工狼群算法,利用该算法有效地解决了轨道小车存取系统复合作业三维空间路径优化问题。针对该系统出入库复合作业的特点,设计了一种包含轨道引导小车任务分配与三维空间路径信息的编... 详细信息

为了提高智能立体仓库的运转效率,提出了一种改进的人工狼群算法,利用该算法有效地解决了轨道小车存取系统复合作业三维空间路径优化问题。针对该系统出入库复合作业的特点,设计了一种包含轨道引导小车任务分配与三维空间路径信息的编码和解码方法;将禁忌表和拥挤度因子引入算法,并采用自适应围攻步长来避免算法陷入局部最优,同时加快算法的后期收敛速度。以某省级电能计量设备自动检定中心的AVS/RS系统为实例,将该算法与另外两种优化算法进行比较,结果表明该改进算法的收敛速度更快、优化率更高、得到的解最优。

关键词：自动小车存取系统改进的人工狼群算法三维空间路径优化

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Trichostatin A Extends the Lifespan of Drosophila melanogaster by Elevating hsp22 Expression

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Acta Biochimica et Biophysica Sinica 2004年第9期36卷 618-622页

作者： Dan TAO#, jun lu#, Hui SUN, Yan-Mei ZHAO, Zhi-Gen YUAN, Xiao-Xue LI, and Bai-Qu HUANG* Institute of Genetics and Cytology, Northeast Normal University, Changchun 130024, China Institute of Genetics and Cytology Northeast Normal UniversityChangchun 130024 China

The level of acetylation of histones in nucleosomes is related to the longevity of yeast and animals. However, the mechanisms by which acetylation and deacetylation affect longevity remain unclear. In present study, we investigated the influence of histone acetylation modification on the expression of hsp22 gene and the lifespan in Drosophila melanogaster using histone deacetylase (HDAC) inhibitor Trichostatin A (TSA). The results showed that TSA could extend the lifespan of Drosophila melanogaster. Furthermore, TSA significantly promoted the hsp22 gene transcription, and affected the chromatin morphology at the locus of hsp22 gene along the polytene chromosome. Present data implicate that TSA may affect the lifespan of Drosophila through changing the level of histone acetylation and influencing the expression of hsp22 gene that is related to aging.

关键词： histone acetylation histone deacetylase inhibitor Drosophila melanogaster hsp22 lifespan

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Integrated transcriptome sequencing and RNA interference reveals molecular changes in Diaphorina citri after exposure to validamycin

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Insect Science 2021年第6期28卷 1690-1707页

作者： Hai-Zhong Yu Yan-Xin Xie Jie Wang Ying Wang Yi-Min Du He-Gui Wang Ba-Lian Zhong Bo Zhu Xiu-Dao Yu Zhan-jun lu College of Life Sciences Gannan Normal UniversityGanzhouJiangxi ProvinceChina National Navel Orange Engineering Research Center GanzhouJiangxi ProvinceChina College of Life Science Anhui Agricultural UniversityHefeiChina

Validamycin has been widely used as a specific competitive inhibitor of *** our previous research,validamycin significantly inhibited trehalase activity and chitin synthesis in Diaphorina citri,resulting in abnormal ***,the mechanism of validamycin's action on *** remains ***,using a comparative transcriptome analysis,464 differentially expressed genes(DEGs)in *** were identified after validamycin treatment.A Gene Ontology enrichment analysis revealed that these DEGs were mainly involved in“small molecule process”,“structural molecule ac-tivity”and“transition metal ion binding”.DEGs involved in chitin metabolism,cuticle synthesis and insecticide detoxification were validated by reverse transcription quantitative polymerase chain *** RNA interference of *** chitinase-like protein EN03 and *** cuticle protein 7 genes significantly affected *** ***,the recombinant chitinase-like protein EN03 exhibited a chitin-binding property,and an antimicrobial activity against Bacillus *** study provides a first insight into the molecular changes in *** after exposure to validamycin and identifies two effective RNA interference targets for *** control.

关键词： chitin-binding Diaphorina citric RNA interference transcriptome sequencing validamycin

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The regulatory mechanism of rapid lignification for timely anther dehiscence

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Journal of Integrative Plant Biology 2024年第8期66卷 1788-1800页

作者： Jing-Shi Xue Yi-Feng Feng Ming-Qi Zhang Qin-Lin Xu Ya-Min Xu jun-Qin Shi Li-fang Liu Xiao-Feng Wu Shui Wang Zhong-Nan Yang Shanghai Key Laboratory of Plant Molecular Sciences College of Life SciencesShanghai Normal UniversityShanghai 200234China National Key Laboratory of Plant Molecular Genetics CAS Center for Excellence in Molecular Plant SciencesInstitute of Plant Physiology and EcologyChinese Academy of SciencesShanghai 200032China University of the Chinese Academy of Sciences Beijing 100049China Development Center of Plant Germplasm Resources College of Life SciencesShanghai Normal UniversityShanghai 200234China

Anther dehiscence is a crucial event in plant reproduction,tightly regulated and dependent on the lignification of the anther *** this study,we investigated the rapid lignification process that ensures timely anther dehiscence in *** findings reveal that endothecium lignification can be divided into two distinct *** Phase Ⅰ,lignin precursors are synthesized without polymerization,while Phase Ⅱ involves simultaneous synthesis of lignin precursors and *** transcription factors MYB26,NST1/2,and ARF17 specifically regulate the pathway responsible for the synthesis and polymerization of lignin monomers in Phase Ⅱ.MYB26-NST1/2 is the key regulatory pathway responsible for endothecium lignification,while ARF17 facilitates this process by interacting with ***,our results demonstrate that the lignification of the endothecium,which occurs within approximately 26 h,is much faster than that of the vascular *** findings provide valuable insights into the regulation mechanism of rapid lignification in the endothecium,which enables timely anther dehiscence and successful pollen release during plant reproduction.

关键词： anther dehiscence ARF17 endothecium lignin MYB26 NST

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Detection of 100% oxygen induced changes in retina using magnetic resonance imaging： a human study

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Chinese Medical Journal 2010年第22期123卷 3277-3281页

作者： XU Qing-gang CHEN Qing-hua XIAN jun-fang WANG Zhen-chang Department of Radiology Beijing Tongren Hospital Capital Medical University Beijing 100730 China

Background Inner retinal oxygenation response （△PO2） is a worldwide study focus. However, the relevant reports on its radiological measurments are limited. In this study, magnetic resonance imaging （MRI）, employing T1 weighted image （T1WI）, was used to detect changes in APO2 following 100% oxygen inhalation in human subjects. Methods MRI was performed on a 1.5-T GE scanner system. After obtaining ophthalmologic data, eleven healthy individuals were given room air and 100% oxygen inhalation in order with different intervals. The MRI TlWl data were collected for 50 minutes. Data were analyzed with NIH IMAGE software. Results APO2 was not panretinally uniform, and changes in oxygenation response were spatially inhomogeneous. During the initial phase （before 5 minutes） of 100% oxygen inhalation, preretinal vitreous water signals in the region of papilla optica increased rapidly. On the contrary, in other regions signals declined. In a later period （35 minutes）, APO2 was panretinally fluctuated and increased slowly and attained homeostasis. After hyperoxia （45 minutes）, delayed-enhancement of preretinal vitreous water signals in regions other than the papilla optica occurred, and then dropped down. There was no significant difference （P 〉0.05） at any consecutive time point during and after hyperoixa. Conclusions These results reveal that hyperoxia can induce region-specific signal changes in preretinal vitreous water. Regulatory activity of the retinal vessel network may be the mechanism during 100% oxygen inhalation. Moreover, MRI is a valuable tool for investigating APO2 and exploring the mechanism of retinal oxygenation response physiologically or pathologically in vivo.

关键词： hyperoxia retina retinal blood flow oxygen measurement magnetic resonance imaging

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Identification of molecular biomarkers for the diagnosis of gastric cancer and lymph-node metastasis

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Gastroenterology Report 2019年第1期7卷 57-66,I0002页

作者： Sharvesh Raj Seeruttun Wing Yan Cheung Wei Wang Cheng fang Zhi-Min Liu Jin-Qing Li Ting Wu jun Wang Chun Liang Zhi-Wei Zhou Department of Gastric Surgery Sun Yat-sen University Cancer CenterGuangzhouGuangdong 510060P.R.China State Key Laboratory of Oncology in South China GuangzhouGuangdong 510060P.R.China Collaborative Innovation Center for Cancer Medicine GuangzhouGuangdong 510060P.R.China Division of Life Science Center for Cancer Research and State Key Lab for Molecular NeuroscienceHong Kong University of Science and TechnologyHong KongP.R.China Fok Ying Tung Research Institute Hong Kong University of Science and TechnologyHong KongP.R.China Biomedical Research Institute Shenzhen-Peking University-Hong Kong University of Science and Technology Medical CenterShenzhenGuangdong 518036P.R.China

Background and objective:Biomarkers are important tools for prompt diagnosis of *** study aimed to identify reliable biomarkers for clinical applications in the diagnosis of gastric cancer and lymph-node(LN)***:Between 1 December 2014 and 31 December 2015,we prospectively collected samples of gastric-cancer tissues,corresponding matched-pair normal gastric mucosa,and their peri-gastric metastatic and non-metastatic LNs to identify quantitatively reliable genes using quantitative real-time polymerase chain *** quantity(RQ)was used to calculate the mRNA expression levels of our target *** were calculated using one-way analysis of variance(ANOVA)and Tukey’s multiple comparison *** graphs were plotted using GraphPad ***:Of nine assessed genes,the mRNA levels of inhibin beta A(INHBA)and secreted phosphoprotein 1(SPP1)were most consistently highly expressed in tumor tissues by 15.4-and 15.6-fold,respectively,as compared with normal tissues(Plusions:Our results showed that INHBA was the most optimally reliable biomarker for diagnosing gastric cancer and LN metastasis.

关键词： Diagnosis gastric cancer lymph node metastasis molecular biomarker

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The effects of co-utilizing green manure and rice straw on soil aggregates and soil carbon stability in a paddy soil in southern China

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Journal of Integrative Agriculture 2023年第5期22卷 1529-1545页

作者： ZHANG Zi-han NIE jun LIANG Hai WEI Cui-lan WANG Yun LIAO Yu-lin lu Yan-hong ZHOU Guo-peng GAO Song-juan CAO Wei-dong College of Resources and Environmental Sciences Nanjing Agricultural UniversityNanjing 210095P.R.China Soil and Fertilizer Institute of Hunan Province Hunan Academy of Agricultural SciencesChangsha 410125P.R.China College of Environment and Ecology Jiangsu Open UniversityNanjing 210036P.R.China Key Laboratory of Plant Nutrition and Fertilizer Ministry of Agriculture and Rural Affairs/Institute of Agricultural Resources and Regional PlanningChinese Academy of Agricultural SciencesBeijing 100081P.R.China

The co-utilization of green manure (GM) and rice straw (RS) in paddy fields has been widely applied as an effective practice in southern ***,its effects on soil aggregate and soil organic carbon (SOC) stability remain *** the present study,the effect of GM,RS,and co-utilization of GM and RS on particle size distribution of soil aggregates and SOC density fractions were measured in a field *** experiment included six treatments,i.e.,winter fallow (WF) without RS return (Ctrl),WF with 50%RS return (1/2RS),WF with 100%RS return (RS),GM without RS return (GM),GM with 50%RS return (GM1/2RS) and GM with 100%RS return (GMRS).The results showed that the proportion of small macro-aggregates (0.25–2 mm) and the mean weight diameter (MWD) of aggregates in the GMRS treatment was greater (by 18.9 and 3.41%,respectively) than in the RS treatment,while the proportion of silt+clay particles (lusion,co-utilizing green manure and rice straw cultivated the SOC pool by increasing the concentration of fLOC and improved soil carbon stability by promoting the sequestration of organic carbon in iPOC as a form of physical protection.

关键词： soil aggregation milk vetch rice straw SoC density fractions C stability paddy soil

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