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Hearing impairment-associated KARS mutations lead to defects in aminoacylation of both cytoplasmic and mitochondrial tRNA^(Lys)

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Science China(Life Sciences) 2020年第8期63卷 1227-1239页

作者： Yong Wang Jing-Bo Zhou Qi-Yu Zeng Siqi Wu Mei-Qin xue Pengfei fang En-Duo Wang Xiao-Long Zhou State Key Laboratory of Molecular Biology CAS Center for Excellence in Molecular Cell ScienceShanghai Institute of Biochemistry and Cell BiologyChinese Academy of SciencesUniversity of Chinese Academy of SciencesShanghai 200031China School of Life Science and Technology ShanghaiTech UniversityShanghai 201210China State Key Laboratory of Bioorganic and Natural Products Chemistry Center for Excellence in Molecular SynthesisShanghai Institute of Organic ChemistryChinese Academy of SciencesShanghai 200032China

Aminoacyl-tRNA synthetases(aaRSs)are ubiquitously expressed,essential enzymes,synthesizing aminoacyl-tRNAs for protein *** defects of aaRSs frequently cause various human *** KARS encodes both cytosolic and mitochondrial lysyl-tRNA synthetases(LysRSs).Previously,two mutations(c.1129 G>A and c.517 T>C)were identified that led to hearing impairment;however,the underlying biochemical mechanism is *** the present study,we found that the two mutations have no impact on the incorporation of LysRS into the multiple-synthetase complex in the cytosol,but affect the cytosolic LysRS level,its tertiary structure,and cytosolic tRNA aminoacylation in *** for mitochondrial translation,the two mutations have little effect on the steady-state level,mitochondrial targeting,and tRNA binding affinity of mitochondrial ***,they exhibit striking differences in charging mitochondrial tRNALys,with the c.517T>C mutant being completely deficient in vitro and in *** constructed two yeast genetic models,which are powerful tools to test the in vivo aminoacylation activity of KARS mutations at both the cytosolic and mitochondrial ***,our data provided biochemical insights into the potentially molecular pathological mechanism of KARS c.1129G>A and c.517T>C mutations and provided yeast genetic bases to investigate other KARS mutations in the future.

关键词： aminoacyl-tRNA synthetase tRNA aminoacylation protein synthesis

Serum pepsinogen Ⅱ is a better diagnostic marker in gastric cancer

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World Journal of Gastroenterology 2012年第48期18卷 7357-7361页

作者： xue-Yuan Cao Zhi-fang Jia Mei-Shan Jin Dong-Hui Cao Fei Kong Jian Suo Jing Jiang Department of Gastrointestinal Surgery Jilin University First Hospital Division of Clinical Epidemiology Jilin University First Hospital Division of Pathology Jilin University First Hospital

AIM:To investigate screening makers for gastric cancer,we assessed the association between gastric cancer and serum pepsinogens(PGs).METHODS:The subjects comprised 450 patients with gastric cancer,111 individuals with gastric atrophy,and 961 healthy *** anti-Helicobacter pylori(***) immunoglobulin G(IgG),PGⅠand PG Ⅱ were detected by enzyme-linked immunosorbent *** atrophy and gastric cancer were diagnosed by endoscopy and histopathological *** ratios and 95%CIs were calculated using multivariate logistic ***:Rates of *** infection remained high in Northeastern *** of *** IgG positivity were greater in the gastric cancer and gastric atrophy groups compared to the control group(69.1% and 75.7% vs 49.7%,P < 0.001).Higher levels of PG Ⅱ(15.9 μg/L and 13.9 μg/L vs 11.5 μg/L,P < 0.001) and lower PGⅠ/PG Ⅱ ratio(5.4 and 4.6 vs 8.4,P < 0.001) were found in patients with gastric cancer or gastric atrophy compared to healthy controls,whereas no correlation was found between the plasma PGⅠconcentration and risk of gastric cancer(P = 0.537).In addition,multivariate logistic analysis indicated that *** infection and atrophic gastritis were independent risk factors for gastric *** plasma PGⅠ/PG Ⅱ ratio was associated with higher risks of atrophy and gastric ***,plasma PG Ⅱ?level?significantly?correlated?with?*** gastric ***:Serum PG Ⅱ concentration and PG Ⅰ/PG Ⅱ ratio are potential biomarkers for *** gastric *** Ⅱ is independently associated with risk of gastric cancer.

关键词： Gastric cancer Pepsinogens Helicobacter pylori Gastric atrophy Screening

KIF18B promotes tumor progression in osteosarcoma by activating β-catenin

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Cancer Biology & Medicine 2020年第2期17卷 371-386页

作者： Tian Gao Ling Yu Zhiwei fang Jiayong Liu Chujie Bai Shu Li Ruifeng xue Lu Zhang Zhichao Tan Zhengfu Fan Key Laboratory of Carcinogenesis and Translational Research(Ministry of Education/Beijing) Department of Bone and Soft Tissue TumorPeking University Cancer Hospital&InstituteBeijing 100142China Department of Orthopedics Renmin Hospital of Wuhan UniversityWuhan 430060China

Objective:Osteosarcoma is a common primary highly malignant bone *** family member 18B(K1F18B)has been identified as a potential oncogene involved in the development and metastasis of several cancer *** KIF18B overexpression in osteosarcoma tissue is clearly detected,its specific function in the disease process remains to be ***:K IF18B expression was assessed in osteosarcoma tissues and *** additionally evaluated the effects of KIF18B on proliferation,migration,and invasion of osteosarcoma cells,both in vitro and in ***:Our results showed overexpression of KIF18B in osteosarcoma tissues and *** of K IF18B induced G1/S phase arrest and significantly inhibited proliferation,migration,and invasion of osteosarcoma cells,both in vitro and in vivo.K IF18B regulated P-catenin expression at the transcriptional level by controlling nuclear aggregation of ATF2 and at the post-transcriptional level by interacting with the adenomatous polyposis coli(APC)tumor suppressor gene in osteosarcoma ***:KIF18B plays a carcinogenic role in osteosarcoma by regulating expression ofβ-catenin transcriptionally via decreasing nuclear aggregation of ATF2 or post-transcriptionally through interactions with *** collective findings support the potential utility of KIF18B as a novel prognostic biomarker for osteosarcoma.

关键词： β-catenin APC ATF2 KIF18B osteosarcoma

Estimation of the potential geographical distribution of a new potato pest(Schrankia costaestrigalis) in China under climate change

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Journal of Integrative Agriculture 2023年第8期22卷 2441-2455页

作者： XIAN Xiao-qing ZHAO Hao-xiang GUO Jian-yang ZHANG Gui-fen LIU Hui LIU Wan-xue WAN fang-hao State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant ProtectionChinese Academy of Agricultural SciencesBeijing100193P.R.China The National Agro-Tech Extension and Service Center Beijing 100193P.R.China

Global food security is threatened by the impacts of the spread of crop pests and changes in the complex interactions between crops and pests under climate *** costaestrigalis is a newly-reported potato pest in southern ***-warning monitoring of this insect pest could protect domestic agriculture as it has already caused regional yield reduction and/or quality decline in potato *** research aimed to confirm the potential geographical distributions(PGDs)of *** in China under different climate scenarios using an optimal MaxEnt model,and to provide baseline data for preventing agricultural damage by *** findings indicated that the accuracy of the optimal MaxEnt model was better than the default-setting model,and the minimum temperature of the coldest month,precipitation of the driest month,precipitation of the coldest quarter,and the human influence index were the variables significantly affecting the PGDs of *** highly-and moderately-suitable habitats of *** were mainly located in eastern and southern *** PGDs of *** in China will decrease under climate *** conversion of the highly-to moderately-suitable habitat will also be significant under climate *** centroid of the suitable habitat area of *** under the current climate showed a general tendency to move northeast and to the middle-high latitudes in the *** agricultural practice of plastic film mulching in potato fields will provide a favorable microclimate for *** in the suitable *** attention should be paid to the early warning and monitoring of *** in order to prevent its further spread in the main areas in China’s winter potato planting regions.

关键词： climate change potential geographical distribution crop insect pests Schrankia costaestrigalis MaxEnt model

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响应面法优化低糖鲜杏果酱配方的研究

保鲜与加工 2024年第7期24卷 71-77页

作者：申雪裴龙英布海丽且姆·阿卜杜热合曼房丹丹朱睿田欢周雨亭新疆理工学院食品科学与工程学院新疆阿克苏843000 新疆黑木耳工程技术研究中心新疆阿克苏843000

为研制口味独特、营养均衡的低糖鲜杏果酱,以新疆赛买提鲜杏为原料,利用单因素试验和响应面试验对低糖鲜杏果酱配方进行优化研究,同时测定低糖鲜杏果酱的理化指标,并与市售果酱进行对比分析。结果表明:杏果在杏果浆中的添加量为40%,以... 详细信息

为研制口味独特、营养均衡的低糖鲜杏果酱,以新疆赛买提鲜杏为原料,利用单因素试验和响应面试验对低糖鲜杏果酱配方进行优化研究,同时测定低糖鲜杏果酱的理化指标,并与市售果酱进行对比分析。结果表明:杏果在杏果浆中的添加量为40%,以杏果浆质量计,白砂糖添加量25%,柠檬酸添加量0.15%,果胶添加量1.0%时得到的鲜杏果酱感官评分(91.60分)最高,果酱杏味浓郁、色泽均匀、口味最佳。采用该工艺配方制作的低糖鲜杏果酱可溶性固形物含量为40.05°Brix,pH为3.53,总酸含量为1.31%,总糖含量为32.15%,水分含量为37.08%,VC含量为5.86 mg/100 g,蛋白质含量为0.65%。研究可为杏果精深加工提供理论参考。

关键词：杏果实果酱低糖配方响应面优化

除草安全剂奈安施用时间对烟草生长发育的影响

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江西农业学报 2012年第12期24卷 78-80页

作者：岳伦勇朱列书廖雪芳尹佳郭东海宋正熊王祖富湖南农业大学农学院湖南长沙410128 中国烟草中南农业试验站湖南长沙410128

研究了不同时间施用除草安全剂奈安,对缓解湖南烟稻水旱轮作中水稻除草剂二氯喹啉酸残留的药害,结果表明:在烟草的农艺性状、生理生化指标、化学成分方面,施用奈安越早,烟草的恢复情况越理想,但最后施用奈安处理的烟草表现出较好的生长... 详细信息

研究了不同时间施用除草安全剂奈安,对缓解湖南烟稻水旱轮作中水稻除草剂二氯喹啉酸残留的药害,结果表明:在烟草的农艺性状、生理生化指标、化学成分方面,施用奈安越早,烟草的恢复情况越理想,但最后施用奈安处理的烟草表现出较好的生长势,烟株生长恢复情况比其他处理略好,此时正处于烟草的生根期,因此奈安的最佳施用时间是烟草的生根期。

关键词：烟草二氯喹啉酸奈安生长发育

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我国天然气年产量与年消费量预测模型建立

辽宁化工 2013年第5期42卷 500-502页

作者：高彦鹏曹奇薛方刚高延新解亚鹏张娜娜宫壮壮西安石油大学陕西西安710065 中石油煤层气有限责任公司韩城分公司陕西韩城715400

通过建立简单的指数增长模型和灰色理论模型对我国天然气产量与消费量进行预测,最后通过实际数据进行了验证对比。预测结果有较高的精度,对天然气用户管理和调度部门有参考意义。

关键词：天然气消费量天然气年产量指数增长模型灰色理论预测

紧凑型鲜食糯玉米新品种‘沪玉糯5号'的选育

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上海农业学报 2016年第3期32卷 160-163页

作者：韩晴潘春丹许梅玲沈新芬沈渊王义发沈雪芳上海市农业科学院作物育种栽培研究所上海201403 浦东新区农业技术推广中心上海201201 嘉定区农业技术推广服务中心上海201800 金山区农业技术推广中心上海201599

‘沪玉糯5号'是以W109为母本、W124为父本杂交选育出的鲜食糯玉米新品种。该品种在上海地区春播出苗至采收85 d,生育期与对照‘苏玉糯5号'相近;产量(去苞叶鲜穗净重)14 665.7 kg/hm^2,比‘苏玉糯5号'增产32.4%。该品种株型紧凑,具有较... 详细信息

‘沪玉糯5号'是以W109为母本、W124为父本杂交选育出的鲜食糯玉米新品种。该品种在上海地区春播出苗至采收85 d,生育期与对照‘苏玉糯5号'相近;产量(去苞叶鲜穗净重)14 665.7 kg/hm^2,比‘苏玉糯5号'增产32.4%。该品种株型紧凑,具有较强的抗性与适应性。

关键词：糯玉米新品种 '沪玉糯5号

蛋白-O-岩藻糖基转移酶1在非小细胞肺癌中的表达及临床意义

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现代预防医学 2024年第12期51卷 2248-2252+2304页

作者：王晓慧李明泰高绿叶郝艳龙冯雪飞翟元芳郭艳琳山西医科大学基础医学院病理教研室山西医科大学基础医学院解剖教研室

目的探讨非小细胞肺癌中蛋白-O-岩藻糖基转移酶1的表达及其临床意义，为非小细胞肺癌的诊断和治疗提供参考依据。方法通过TCGA、UALCAN数据库和免疫组化技术探讨POFUT1 mRNA和POFUT1蛋白在非小细胞肺癌的表达情况。提取TCGA数据库的... 详细信息

目的探讨非小细胞肺癌中蛋白-O-岩藻糖基转移酶1的表达及其临床意义，为非小细胞肺癌的诊断和治疗提供参考依据。方法通过TCGA、UALCAN数据库和免疫组化技术探讨POFUT1 mRNA和POFUT1蛋白在非小细胞肺癌的表达情况。提取TCGA数据库的临床数据，采用Kaplan-Meier法进行生存分析，采用COX比例风险回归模型分析POFUT1 mRNA表达水平对非小细胞肺癌患者预后的影响。结果 POFUT1 mRNA和POFUT1蛋白在肺腺癌和肺鳞癌组织中均高表达(均P<0.05)。肺腺癌中POFUT1 mRNA的表达水平与临床分期相关(P<0.001),肺鳞癌中POFUT1 mRNA的表达与肿瘤直径相关(P<0.001)。COX回归分析结果显示，POFUT1 mRNA高表达(P=0.012)、肿瘤直径、临床分期、淋巴结转移与肺腺癌患者预后相关(均P<0.001),临床Ⅲ-Ⅳ期是肺腺癌患者独立危险因素。肿瘤直径(P=0.001)、临床Ⅲ-Ⅳ期(P=0.006)和POFUT1 mRNA高表达(P=0.049)与肺鳞癌患者预后相关，肿瘤直径大于5cm和POFUT1高表达是肺鳞癌患者独立危险因素。生存分析结果显示POFUT1高表达的非小细胞肺癌患者生存率显著低于低表达患者。结论 POFUT1在非小细胞肺癌组织中高表达，且其高表达与患者不良预后相关，可作为非小细胞肺癌预后评估的生物标志物。

关键词：非小细胞肺癌 POFUT1 生物信息学免疫组织化学预后