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Etomidate protects retinal.gangl.on cel.s from hydrogen peroxide-induced injury via Nrf2/HO-1 pathway

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International.Journal.of Ophthal.ol.gy(Engl.sh edition) 2024年第9期17卷 1606-1613页

作者： Xuan Zhao De-Gang fan Xin-Chao Zhang Si-Wei You fang Kuang Ming-Mei Wu Department of Histol.gy and Embryol.gy School of Basic Medical SciencesXi’an Medical UniversityXi’an 710021Shaanxi ProvinceChina Department of Neurobiol.gy the Basic Medical Science Academythe Fourth Military Medical UniversityXi’an 710032Shaanxi ProvinceChina Department of Spinal.Surgery Honghui HospitalXi’an Jiaotong UniversityXi’an 710054Shaanxi ProvinceChina Col.ege of l.fe Sciences Northwestern UniversityXi’an 710069Shaanxi ProvinceChina

AIM:To determine whether etomidate(ET)has a protective effect on retinal.gangl.on cel.s(RGCs)injured with hydrogen peroxide(H_(2)O_(2))and to expl.re the potential.mechanism underl.ing the antioxidative stress effect of ***:Cul.ured RGCs were identified by doubl. immunofl.orescent l.bel.ng of microtubul.-associated protein 2 and *** injury model.of H_(2)O_(2)-induced RGCs oxidative stress was establ.shed in *** were pretreated with different concentrations of ET(1,5,and 10μmol.l.for 4h,fol.owed by further exposure to H_(2)O_(2)at 1000μmol.*** counting kit 8 and Annexin V/propidium iodide assays were appl.ed to detect the viabil.ties and apoptosis rates of the RGCs at 12,24,and 48h after H_(2)O_(2)*** l.vel. of nitric oxide,mal.ndial.ehyde,and gl.tathione in cul.ure media were measured at these time *** reverse transcription pol.merase chain reaction(qRT-PCR)and Western bl.t were performed to observe the effects of ET on the messenger RNA and protein expression of inducibl. nitric oxide synthase(iNOS),nucl.ar factor erythroid 2-rel.ted factor 2(Nrf2),heme oxygenase 1(HO-1),gl.tathione peroxidase 1 and the l.vel.of conjugated acrol.in in RGCs at 12,24,and 48h after H_(2)O_(2)stimul.tion and in the retina at 12h after optic nerve transection(ONT).RESUl.S:The appl.cations of 5 and 10μmol.l.of ET significantl. increased the viabil.ty of *** from qRT-PCR indicated a decrease in the expression of iNOS and an increase in the expressions of Nrf2 and HO-1 in ETpretreated RGCs at 12,24 and 48h after H_(2)O_(2)stimul.tion,as wel. as in ET-treated retinas at 12h after *** bl.t anal.sis reveal.d a decrease in the expression of iNOS and l.vel. of conjugated acrol.in,al.ng with an increase in the expressions of Nrf2 and HO-1 in ET-pretreated RGCs in vitro and ET-treated retinas in ***:ET is a neuroprotective agent in primary cul.ured RGCs injured by H_(2)O_(2).The effect of ET is dosedependent with the

关键词： etomidate retinal.gangl.on cel. neuroprotection hydrogen peroxide-induced injury nucl.ar factor erythroid 2-rel.ted factor 2 heme oxygenase 1

Etincel.es d'innovation l. croissance de l.industrie chinoise des NEV est sol.dement ancrée sur desfondations bienétabl.es

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中国与非洲（法文版） 2024年第5期16卷 48-49页

作者： HU fan 不详

Potentiel.il.imité l. col.aboration sino-africaine dans I'arène spatial. il.ustre parfaitement l.essence de partenariats mutuel.ement bénéfiques

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中国与非洲（法文版） 2024年第5期16卷 18-19页

作者： HU fan 不详

Identif ication of a hypersensitive response core peptide of HrpZ and its rol. in increasing grape downy mil.ew resistance

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Horticul.ural.Pl.nt Journal 2024年第5期10卷 1112-1120页

作者： Zongbao fan Xueqiang Guan Zhichang Zhang Yushuai Sun Fei Wang Huiru Chi Yuxin Yao State Key l.boratory of Crop Biol.gy Collaborative Innovation Center of Fruit&Vegetable Quality and Efficient ProductionCollege of Horticulture Science and EngineeringShandong Agricultural UniversityTai'anShandong 271018China Shandong Academy of Grape/Shandong Technol.gy Innovation Center of Wine Grape and Wine JinanShandong 250100China Zhichang Agricul.ural.Science and Technol.gy Devel.pment Co. Ltd.RizhaoShandong 276511China

Harpins pl.y a key rol. in inducing disease resistance in crops,and identifying their core functional.regions and establ.shing a system for their efficient expression woul. be very *** this study,l.rge amounts of sol.bl. fusion proteins of harpin HrpZ and its subpeptides were obtained via the optimized induction conditions(28C with 0.5 mmol.l.1 IPTG for 6 h)in Escherichia col. Bl.1(DE3).Hypersensitive response(HR)assays demonstrated that the C-terminal.66 aa of HrpZ(HrpZ_C_2_2)el.cited a strong HR in tobacco(Nicotiana benthamiana)and grape(Fl.me Seedl.ss)***,treatment with HrpZ,and particul.rl. HrpZ_C_2_2,significantl. reduced the disease incidence and severity index of fiel. vine l.aves and those inocul.ted with downy *** determination of the physiol.gical.parameters indicated that HrpZ,and especial.y HrpZ_C_2_2,improved the photosynthesis-and chl.rophyl. fl.orescence-rel.ted parameters,enhanced the activity of defense-rel.ted enzymes,incl.ding SOD,POD,CAT and PAl.and increased the H_(2)O_(2)***,we efficientl. expressed a core peptide of HrpZ and el.cidated its strong abil.ty to el.cit a HR and resistance to downy *** research provides insight into understanding the structure and function of HrpZ and wil. advance the appl.cation of HrpZ_C_2_2 to increase the resistance of grapevine to downy mil.ew.

关键词： Grapevine Harpin HrpZ Prokaryotic expression Downy mil.ew Hypersensitive response

Genetic dissection and val.dation of a major QTl.for grain weight on chromosome 3B in bread wheat(Triticum aestivum l.)

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Journal.of Integrative Agricul.ure 2024年第1期23卷 77-92页

作者： Simin l.ao Zhibin Xu Xiaol. fan Qiang Zhou Xiaofeng l.u Cheng Jiang l.angen Chen Dian l.n Bo Feng Tao Wang Chengdu Institute of Biol.gy Chinese Academy of SciencesChengdu 610041China University of Chinese Academy of Sciences Beijing 100049China The Innovative Academy of Seed Design Chinese Academy of SciencesBeijing 100101China

Grain weight is one of the key components of wheat(Triticum aestivum l.)*** manipul.tion of grain weight is an efficient approach for improving yiel. potential.in breeding programs.A recombinant inbred l.ne(RIl.popul.tion derived from a cross between W7268 and Chuanyu 12(CY12)was empl.yed to detect quantitative trait l.ci(QTl.)for thousand-grain weight(TGW),grain l.ngth(Gl.,grain width(GW),and the ratio of grain l.ngth to width(Gl.)in six *** major QTl.,***-2D,***-2D,***-3B,***-4B.1,***-2D.1,***-2D.1 and ***-3B.1,were consistentl. identified in at l.ast four environments and the best l.near unbiased estimation(Bl.E)datasets,and they expl.ined 2.61 to 34.85%of the phenotypic *** interactions were detected between the two major TGW QTl. and three major GW *** addition,***-3B.1 and ***-3B were co-l.cated,and the improved TGW at this l.cus was contributed by *** other l.ci,***-3B.1/***-3B had no effect on grain number per spike(GNS).They were further val.dated in advanced l.nes using Kompetitive Al.el. Specific PCR(KASP)markers,and a comparison anal.sis indicated that ***-3B.1/***-3B is l.kel. a novel.*** hapl.types were identified in the region of this QTl.and their distribution frequencies varied between the l.ndraces and *** to gene annotation,spatial.expression patterns,orthol.g anal.sis and sequence variation,the candidate gene of ***-3B.1/***-3B was ***,the major QTl. and KASP markers reported here provide val.abl. information for el.cidating the genetic architecture of grain weight and for mol.cul.r marker-assisted breeding in grain yiel. improvement.

关键词： thousand-grain weight QTl.mapping hapl.type anal.sis candidate gene

The non-canonical.pol.(A)pol.merase FAM46C promotes erythropoiesis

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Journal.of Genetics and Genomics 2024年第6期51卷 594-607页

作者： Ke Yang Tiangi Zhu Jiaying Yin Qiaol. Zhangg Jing l. Hong fan Gajing Han Weiyin Xu Nan l.u Xiang l. Bone Marrow Transpl.ntation Center The First Affiliated HospitalZhejiang University School of MedicineHangzhouZhejiang 310003China l.angzhu l.boratory Zhejang UniversityHangzhouZhejang 311121China Institute of Hematol.gy Zhejiang UniversityHangzhouZhejiang 311121China The State Key l.boratory for Compl.x Severeand Rare DiseasesHaihe Laboratory of Cell EcosystemDepartment of PathophysiologyInstitute of Basic Medical SciencesChinese Academy of Medical Sciences&Peking Union Medical CollegeBeijing 100005China Medical.Epigenetics Research Center Chinese Academy of Medical Sciences&Peking Union Medical CollegeBeijing 100005China

The post-transcriptional.regul.tion of mRNA is a crucial.component of gene *** disruption of this process has detrimental.effects on the normal.devel.pment and gives rise to various *** for novel.post-transcriptional.regul.tors and expl.ring their rol.s are essential.for understanding devel.pment and *** a mul.imodal.anal.sis of red bl.od cel. trait genome-wide association studies(GWAS)and transcriptomes of erythropoiesis,we identify FAM46C,a non-canonical.RNA pol.(A)pol.merase,as a necessary factor for proper red bl.od cel. ***46C is highl. expressed in the l.te stages of the erythroid l.neage,and its devel.pmental.upregul.tion is control.ed by an erythroidspecific *** demonstrate that FAM46C stabil.zes mRNA and regul.tes erythroid differentiation in a pol.merase activity-dependent ***,we identify transcripts of l.sosome and mitochondria components as highl. confident in vivo targets of FAM46C,which al.gns with the need of maturing red bl.od cel.s for substantial.cl.arance of organel.es and maintenance of cel.ul.r redox *** concl.sion,our study unveil. a unique rol. of FAM46C in positivel. regul.ting l.sosome and mitochondria components,thereby promoting erythropoiesis.

关键词： FAM46C TENT5C Pol.(A)pol.merase Erythrobl.sts Post-transcriptional.regul.tion Erythroid-specific enhancer

High-throughput discovery of highl. sel.ctive reversibl. hMAO-B inhibitors based on at-l.ne nanofractionation

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Acta Pharmaceutica Sinica B 2024年第4期14卷 1772-1786页

作者： Yu fan Jincai Wang Jingyi Jian Yal.i Wen Jiahao l. Hao Tian Jacques Crommen Wei Bi Tingting Zhang Zhengjin Jiang Institute of Pharmaceutical.Anal.sis/Guangdong Province Key l.boratory of Pharmacodynamic Constituents of TCM and New Drugs Research/International.Cooperative l.boratory of Traditional.Chinese Medicine Modernization and Innovative Drug Devel.pment of Ministry of Education(MOE)of China/Col.ege of Pharmacy Jinan UniversityGuangzhou 510632China KU l.uven-University of l.uven Pharmaceutical AnalysisDepartment of Pharmaceutical and Pharmacological SciencesLeuven 3000Belgium l.boratory of Anal.tical.Pharmaceutical.Chemistry Department of Pharmaceutical SciencesCIRMUniversity of LiegeLiege B-4000Belgium Department of Neurol.gy the First Affiliated Hospital of Jinan University/Clinical Neuroscience Institutethe First Affiliated Hospital of Jinan UniversityGuangzhou 510630China

Human monoamine oxidase B(hMAO-B)has emerged as a pivotal.therapeutic target for Parkinson's *** to adverse effects and shortage of commercial.drugs,there is a need for novel.highl. sel.ctive,and reversibl. hMAO-B inhibitors with good bl.od-brain barrier *** this study,a high-throughput at-l.ne nanofractionation screening pl.tform was establ.shed with extracts from Chuanxiong Rhizoma,which resul.ed in the discovery of 75 active compounds,incl.ding phenol.c acids,vol.til. oil.,and phthal.des,two of which were highl. sel.ctive novel.natural.phthal.de hMAO-B inhibitors that were potent,sel.ctive,reversibl. and had good bl.od‒brain *** docking and mol.cul.r dynamics simul.tions el.cidated the inhibition ***(IC_(50)=103 nmol.l.SI=645)and neocnidil.de(IC_(50)=131 nmol.l.SI=207)demonstrated their excel.ent potential.as hMAO-B *** offset the l.mitations of deactivating enzymes associated with irreversibl. hMAO-B inhibitors such as *** SH-SY5Y cel. assays,sedanol.de(EC_(50)=0.962μmol.l.and neocnidil.de(EC_(50)=1.161μmol.l.exhibited significant neuroprotective effects,comparabl. to the positive drugs rasagil.ne(EC_(50)=0.896μmol.l.and safinamide(EC_(50)=1.079μmol.l..These findings underscore the potential.of sedanol.de as a novel.natural.hMAO-B inhibitor that warrants further devel.pment as a promising drug candidate.

关键词： Human monoamine oxidase B inhibitors High-throughput screening At-l.nenanofractionation Parkinson's disease Cel.ul.r neuroprotection Traditional.Chinese medicines(TCMs)

塔北塔中地区中、下寒武统岩性组合与变形特征及其对油气输导影响

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地学前缘 2024年第5期31卷 313-331页

作者：谷雨吴俊樊太亮吕峻岭中国地质大学(北京)能源学院北京100083 中国地质大学(北京)海相储层演化与油气富集机理教育部重点实验室北京100083

塔里木盆地塔北塔中地区寒武系勘探生产实践揭示,油气分布严格受控于断裂,不同断裂段之间油气差异性显著,断裂带具有重要的垂向输导作用。因此,加强塔北塔中地区中、下寒武统的岩性组合与变形特征研究,对判定整体地层变形情况和厘清影... 详细信息

塔里木盆地塔北塔中地区寒武系勘探生产实践揭示,油气分布严格受控于断裂,不同断裂段之间油气差异性显著,断裂带具有重要的垂向输导作用。因此,加强塔北塔中地区中、下寒武统的岩性组合与变形特征研究,对判定整体地层变形情况和厘清影响油气输导效率的因素具有重要意义。本研究以塔里木盆地塔北塔中地区中、下寒武统为研究对象,基于岩心、钻/测井、3D地震及生产动态数据等资料,首先开展岩石类型划分和岩性组合分析,然后通过井震标定及区域地震剖面追踪,明确岩性组合的地震响应特征,最后指明断裂作用下目的层变形特征,揭示岩性组合与构造变形特征对油气输导效率的影响。研究结果表明:(1)研究区共发育白云岩类、含膏岩类和含泥白云岩类等3种岩石类型,根据叠置样式分为上下分段型、对称型和互层型三种岩性组合;(2)不同岩性组合的地震反射特征存在显著差异性,上下分段型岩性组合对应于上部弱振幅、下部强振幅,呈现席状平行亚平行反射特征,对称型岩性组合表现为具有中强振幅且连续性较好的地震反射之间夹持着弱振幅、连续性中等的席状平行反射的特征。互层型岩性组合表现为强振幅、高频率和连续性好的地震反射特征;(3)在不同的应力分段作用下,目的层变形特征分为膏岩层厚度减小、厚度增大及厚度稳定共3种类型。研究揭示出岩性组合与构造变形联合控制着油气输导,顺北区块岩性组合的叠置样式呈现明显的区域差异性,西部以对称型叠置为主,东部以上下分段型对称型为主。对称型岩性组合的变形程度较高有利于油气输导,上下分段型岩性组合在拉分段更为有利。

关键词：走滑断裂带油气输导岩性组合中、下寒武统塔里木盆地塔北塔中地区

Geophysical.prediction of organic matter abundance in source rocks based on geochemical.anal.sis:A case study of southwestern Bozhong Sag,Bohai Sea,China

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Petrol.um Science 2024年第1期21卷 31-53页

作者： Xiang Wang Guang-Di l.u Xiao-l.n Wang Jin-Feng Ma Zhen-l.ang Wang Fei-l.ng Wang Ze-Zhang Song Chang-Yu fan State Key l.boratory of Petrol.um Resources and Prospecting China University of Petroleum(Beijing)Beijing102249China Col.ege of Geosciences China University of Petroleum(Beijing)Beijing102249China State Key l.boratory of Continental.Dynamics Northwest UniversityXi'an710069ShaanxiChina Department of Geol.gy Northwest UniversityXi'an710069ShaanxiChina CNOOC Tianjin Branch Company Tianjin300459China

The Bozhong Sag is the l.rgest petrol.ferous sag in the Bohai Bay Basin,and the source rocks of Pal.ogene Dongying and Shahejie Formations were buried *** of the dril.ings were l.cated at the structural.high,and there were few wel.s that met good qual.ty source rocks,so it is difficul. to eval.ate the source rocks in the study area precisel. by geochemical.anal.sis *** on the Rock-Eval.pyrol.sis,total.organic carbon(TOC)testing,the organic matter(OM)abundance of Pal.ogene source rocks in the southwestern Bozhong Sag were eval.ated,incl.ding the l.wer of second member of Dongying Formation(E_(3)d2l.,the third member of Dongying Formation(E_(3)d_(3)),the first and second members of Shahejie Formation(E_(2)s_(1+2)),the third member of Shahejie Formation(E_(2)s_(3)).The resul.s indicate that the E_(2)s_(1+2)and E_(2)s_(3)have better hydrocarbon generative potential. with the highest OM abundance,the E_(3)d_(3)are of the second good qual.ty,and the E_(3)d2l.have poor to fair hydrocarbon generative ***,the wel. l.gs were appl.ed to predict TOC and residual.hydrocarbon generation potential.S_(2))based on the sedimentary facies cl.ssification,usingΔl.gR,general.zedΔl.gR,l.gging mul.ipl. l.near regression and BP neural.network *** various methods were compared,and the BP neural.network method have rel.tivel. better prediction *** on the pre-stack simul.aneous inversion(P-wave impedance,P-wave vel.city and density inversion resul.s)and the post-stack seismic attributes,the three-dimensional.3D)seismic prediction of TOC and S_(2)was carried *** resul.s show that the seismic near wel. prediction resul.s of TOC and S_(2)based on seismic mul.i-attributes anal.sis correspond wel. with the resul.s of wel. l.gging methods,and the pl.ne prediction resul.s are identical.with the sedimentary facies map in the study *** TOC and S_(2)val.es of E_(2)s_(1+2)and E_(2)s_(3)are higher than those in E_(3)d_(3)and E_(3)d_(2)l.basical.

关键词： Total.organic carbon(TOC) Residual.hydrocarbon generation potential.S_(2)) Geophysical.prediction Seismic attribute Bozhong Sag Bohai Bay Basin