Genetic dissection and validation of a major QTL for grain weight on chromosome 3B in bread wheat(Triticum aestivum L.)

作     者：Simin Liao Zhibin Xu Xiaoli Fan Qiang Zhou Xiaofeng Liu Cheng Jiang Liangen Chen Dian Lin Bo Feng Tao Wang Simin Liao;Zhibin Xu;Xiaoli Fan;Qiang Zhou;Xiaofeng Liu;Cheng Jiang;Liangen Chen;Dian Lin;Bo Feng;Tao Wang

作者机构：Chengdu Institute of BiologyChinese Academy of SciencesChengdu 610041China University of Chinese Academy of SciencesBeijing 100049China The Innovative Academy of Seed DesignChinese Academy of SciencesBeijing 100101China 

出 版 物：《Journal of Integrative Agriculture》 (农业科学学报（英文版）)

年 卷 期：2024年第23卷第1期

页      面：77-92页

核心收录：

学科分类：09[农学] 0901[农学-作物学] 

基　　金：supported by the Major Program of National Agricultural Science and Technology of China(NK20220607) the West Light Foundation of the Chinese Academy of Sciences(2022XBZG_XBQNXZ_A_001) the Sichuan Science and Technology Program,China(2022ZDZX0014) 

主　　题：thousand-grain weight QTL mapping haplotype analysis candidate gene 

摘      要：Grain weight is one of the key components of wheat(Triticum aestivum L.)*** manipulation of grain weight is an efficient approach for improving yield potential in breeding programs.A recombinant inbred line(RIL)population derived from a cross between W7268 and Chuanyu 12(CY12)was employed to detect quantitative trait loci(QTLs)for thousand-grain weight(TGW),grain length(GL),grain width(GW),and the ratio of grain length to width(GLW)in six *** major QTLs,***-2D,***-2D,***-3B,***-4B.1,***-2D.1,***-2D.1 and ***-3B.1,were consistently identified in at least four environments and the best linear unbiased estimation(BLUE)datasets,and they explained 2.61 to 34.85%of the phenotypic *** interactions were detected between the two major TGW QTLs and three major GW *** addition,***-3B.1 and ***-3B were co-located,and the improved TGW at this locus was contributed by *** other loci,***-3B.1/***-3B had no effect on grain number per spike(GNS).They were further validated in advanced lines using Kompetitive Allele Specific PCR(KASP)markers,and a comparison analysis indicated that ***-3B.1/***-3B is likely a novel *** haplotypes were identified in the region of this QTL and their distribution frequencies varied between the landraces and *** to gene annotation,spatial expression patterns,ortholog analysis and sequence variation,the candidate gene of ***-3B.1/***-3B was ***,the major QTLs and KASP markers reported here provide valuable information for elucidating the genetic architecture of grain weight and for molecular marker-assisted breeding in grain yield improvement.