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Hydrolytic and Antimicrobial Activities of Bacteria Isolated from Fermented Peppers Sold in Markets at Brazzaville, Republic of the Congo

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Journal of Biosciences and Medicines 2024年第9期12卷 215-234页

作者： faly armel soloka mabika Elgie Viennechie Gatsé Ngo Itsouhou Tarcisse Baloki Ngoulou Mérite Berthelie Mouanda Nzoussi Nguimbi Etienne Laboratory of Cell and Molecular Biology Faculty of Sciences and Techniques Marien Ngouabi University Brazzaville Republic of the Congo Unit of Molecular Microbiology and Bioinformatics Faculty of Sciences and Techniques Marien Ngouabi University Brazzaville Republic of the Congo

Microorganisms are omnipresent in all environments and play mainly the role of transformers, thanks to the multiple enzymes they are able to produce. In order to valorize fermented foods in the Republic of the Congo, this work aimed to characterize and study some properties of microorganisms isolated from samples of peppers sold in three markets of Brazzaville. A numeration of the total aerobic mesophilic flora (TAMF) was made in a solid medium, allowing the evaluation of each sample’s microbial concentration. The microbial mass varied from 2.8 × 105 CFU/g for the Ouénzé sample to 1.8 × 104 CFU/g for the Total sample and 2 × 104 CFU/g for the Moungali market sample. The evaluation of the enzymatic properties of the Bacillus isolates showed that 68.42% were capable of producing cellulases and 78.94% were capable of producing amylases and proteases. Antimicrobial activities revealed that 63.15% of the isolates were able to secrete inhibitory substances against E. coli and Staphylococcus aureus. Molecular analysis by PCR amplification, sequencing of the 16S rRNA gene and BLAST bioinformatics analysis provides newly identified bacteria strains with new accession numbers in GenBank: Bacillus thuringiensis MBCBR322 (OP474008), Bacillus megaterium MBCBJ1822 (OP476493), Bacillus thuringiensis MBCBR222 (OP476494), Priestia megaterium MBCBJ2022 (OP476495) and Lactobacillus paraplantarum MBCBR1522 (OP476496). Multiple sequences alignment of identified sequences with their homologs of GenBank has shown high similarities. The phylogenetic inference assay has provided the two groups of strains observed in this study, and the two groups are very coherent with the phylogeny of the reference.

关键词： Antimicrobial Activity Hydrolytic Activity Sequencing Pepper Brazzaville

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16S rRNA Gene-Based Metagenomic Analysis of Soil Bacterial Diversity in Brazzaville, Republic of the Congo

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Advances in Microbiology 2023年第9期13卷 477-498页

作者： Irène Marie Cécile Mboukou Kimbatsa Itsouhou Ngô armel Ibala Zamba faly armel soloka mabika Thantique Moutali Lingouangou Joseph Goma-Tchimbakala Etienne Nguimbi Laboratoire de Biologie Cellulaire et Moléculaire (BCM) Faculté des Sciences et Techniques Université Marien Ngouabi Brazzaville Republic of the Congo Unité de Recherche en Bioinformatique et Microbiologie Moléculaire Faculté des Sciences et Techniques Université Marien Ngouabi Brazzaville Republic of the Congo Institut National de Recherche en Sciences Exactes et Naturelles (IRSEN) Brazzaville Republic of the Congo

Soil contains a great diversity of microorganisms, among which are bacteria. This study aimed to explore bacterial diversity in soil samples in Brazzaville in the Republic of the Congo. Environmental DNA was extracted. The illumina MiSeq sequencing was held and the diversity indices have been computed. Illumina MiSeq sequencing revealed 21 Phyla, four of which were abundant: Proteobacteria, Acidobacteria, Actinobacteria and Bacteroidetes. Soil microbial communities in the studied samples were phylogenetically diverse but with a stable community structure. 17 classes are represented with relative abundances of Rihzobiales, Bacillales, Actinomycetales and Acidobacteriales. 40 families, the Alphaproteobacteria, the Bacilli and the 12 Actinobacteria. 83 orders among which the Rhizobiales are the most abundant followed by Bacillales and the least abundant followed by the Flavobacteriaceae. Of the 28 genera listed, the Bradyrhizobium is the most dominant in Mw3 and Mw4. 25 listed species, Bradyrhizobium, Bacillus, Actinoplanes, and Candidatu coribacter Acidobacterium are the most abundant species. The Shannon indices of Mw3 and Mw4 are equal, the H’max of Mw4 is greater than the H’max of Mw3. The Simpson index of Mw4 is equal to the Simpson index of Mw3, and the Pielou index (J) of Mw4 is less than the R of Mw3, but very close. This study opens interesting perspectives on the knowledge and exploitation of telluric bacteria in several areas of life.

关键词： Metagenomic Sequencing 16S rRNA Gene Soil Bacteria

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Optimization of Cellulase Production Conditions in Bacteria Isolated from Soils in Brazzaville

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Journal of Biosciences and Medicines 2022年第8期10卷 14-28页

作者： Thantique Moutali Lingouangou Raoul Ampa Etienne Nguimbi Prince Moukouyou Bissombolo Ngo-Itsouhou faly armel soloka mabika Tarcisse Baloki Ngoulou Stech Anomene Eckzechel Nzaou Laboratoire de Biologie Cellulaire et Moléculaire Faculté des Sciences et Techniques Université Marien Ngouabi Brazzaville République du Congo Unité de Microbiologie Moléculaire et Bioinformatique Faculté des Sciences et Techniques Université Marien Ngouabi Brazzaville Republique du Congo Institut National de Recherches en Sciences Exactes et Naturelles (IRSEN) Brazzaville République du Congo

Objective: The objective of the present study is to optimize cellulase production in five strains: (Pantoea dispersa MLTBY6 (MT646430.1);Pseudomonas aeruginosa MLTBM2 (MT646431.1);Pseudomonas monteilii MLTBC10 (MT674682.1);Bacillus subtilis MLTBC5 (MT674681.1) and Lysinibacillus fusiformis MLTBB7 selected cellulase producers isolated from soils in Brazzaville, Republic of the Congo. Materials and Methods: The cellulolytic activity of the selected cellulase-producing strains was determined by transferring the strains to a petri dish containing CMC culture medium with the following composition: cellulose 1%, K2HPO4 0.2%, MgSO4 0.03%, peptone 1%, (NH4)2SO4 0.2% adjusted to a pH value of 7, previously poured and then frozen. The dishes were incubated in an oven at 37°C for 48 hours. The petri dishes were then flooded with 1% lugol for 15 minutes. A positive reading is indicated by the formation of a hydrolysis zone, the diameters of the hydrolysis zone were measured with a ruler. Strains with a broad lysis spectrum were selected. Optimisation of cellulase production by five bacterial strains isolated from the soil was done using the following factors: temperature and pH. Results: The production of cellulase showed that these strains showed a high production of cellulase at pH values between 5.6 and 9 with an optimum of pH = 8 and temperature values between 35°C and 40°C with an optimum at temperature t = 40°C. Of the carbon sources used, two sources, namely glucose and galactose, showed a high production of cellulase compared to the other carbon sources. However, the two nitrogen sources, ammonium sulphate and urea, were favourable for cellulase production by all five strains. Fe2+, CO2+, Zn2+ ions are favourable for cellulase production by these strains, with a referendum for Fe2+. Conclusion: From these results, we conclude that the sources of carbon (gluc

关键词： Optimization Condition Production Cellulase Soil Bacteria

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16S rRNA Gene-Based Metagenomic Analysis of Soil Bacterial Diversity in Brazzaville,Republic of the Congo

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Advances in Microbiology（微生物学） 2023年第9期

作者： Irène Marie Cécile Mboukou Kimbatsa Itsouhou Ngô armel Ibala Zamba faly armel soloka mabika Thantique Moutali Lingouangou Joseph Goma-Tchimbakala Etienne Nguimbi

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Antibiotic Resistance Profile of Pathogenic Bacteria Isolated from “Mabokés” Smothered Fish in Brazzaville, Congo

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Journal of Biosciences and Medicines 2020年第8期8卷 138-148页

作者： Morabandza Cyr Jonas Nguimbi Etienne Baloki Ngoulou Tarcisse Moyen Rachel Moussounda Milongo Lucien Jolivet soloka mabika armel faly Ngo-Itsouhou Christian Ahombo Gabriel Normal Superior School Marien NGOUABI University Brazzaville Republic of Congo Cellular and Molecular Biology Laboratory Faculty of Sciences and Techniques Marien NGOUABI University Brazzaville Republic of Congo

The smothered fish samples were taken from 3 markets. They were grown on different selective and differentiated culture media to target groups of bacteria associated with food poisoning. Isolates were identified on the basis of cellular and colonial morphologies on selective and differentiated culture media, followed by susceptibility testing to certain families of antibiotics, in particular beta-lactams. This study showed that S. aureus and B. cereus had high levels of beta-lactam resistance. However, these strains were sensitive to kanamycin, tobramycin, ciprofloxacin and norfloxacin. The characteristic penicillinase phenotype was dominant in Gram-positive bacteria. Shigella spp, Salmonella spp and E. coli were resistant to beta-lactam antibiotics. Tobramycin and meropenem retained their activity on all strains. Despite the increased rates of resistance observed, vancomycin, kanamycin, tobramycin, gentamicin, ciprofloxacin, and norfloxacin can be used in the treatment of community-acquired infections caused by Gram-positive bacteria while meropenem and tobramycin for Shigella spp, Salmonella spp and E. coli infections.

关键词： Resistance Antibiotics Bacteria Smothered Fish

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