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New guidelines for fluorophore application in peroxisome targeting analyses in transient plant expression systems

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Journal of Integrative Plant Biology 2019年第7期61卷 884-899页

作者： Christian falter Nguyen Binh Anh Thu Saugat Pokhrel Sigrun Reumann Plant Biochemistry and Infection Biology Institute of Plant Science and Microbiology University of Hamburg

Peroxisome research has been revolutionized by proteome studies combined with in vivo subcellular targeting analyses. Yellow and cyan fluorescent protein(YfP and CfP) are the classical fluorophores of plant peroxisome research. In the new transient expression system of Arabidopsis seedlings co-cultivated with Agrobacterium we detected the YfP fusion of one candidate protein in peroxisomes, but only upon co-transformation with the peroxisome marker, CfP-PTS1. The data suggested that the YfP fusion was directed to peroxisomes due to its weak heterodimerization ability with CfP-PTS1,allowing piggy-back import into peroxisomes. Indeed, if co-expressed with monomeric Cerulean-PTS1(mCer-PTS1),the YfP fusion was no longer matrix localized. We systematically investigated the occurrence and extent of dimerization-based piggy-back import for different fluorophore combinations in five major transient plant expression systems. In Arabidopsis seedlings and tobacco leaves both untagged YfP and monomeric Venus were imported into peroxisomes if co-expressed with CfP-PTS1 but not with mCer-PTS1. By contrast, piggy-back import of cytosolic proteins was not observed in Arabidopsis and tobacco protoplasts or in onion epidermal cells for any fluorophore combination at any time point. Based on these important results we formulate new guidelines for fluorophore usage and experimental design to guarantee reliable identification of novel plant peroxisomal proteins.

关键词： transient plant expression systems fluorophore

How non-rapid eye movement sleep and Alzheimer pathology are linked

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World Journal of Psychiatry 2021年第11期11卷 1027-1038页

作者： Annelies falter Maarten J A Van Den Bossche Department of Geriatric Psychiatry University Psychiatric Center KU LeuvenLeuven 3000Belgium Center for Neuropsychiatry Research Group PsychiatryDepartment of NeurosciencesLeuven Brain InstituteKU LeuvenLeuven 3000Belgium

Alzheimer's disease(AD)is a multifactorial neurodegenerative disorder characterized by the presence of senile plaques and neurofibrillary *** attempts to identify characteristic factors that are associated with the presence of the AD pathology on the one hand and that increase the risk of developing AD on the *** in non-rapid eye movement(NREM)sleep may meet both requirements for various ***,NREM-sleep is important for optimal memory *** addition,studies report that the presence of AD pathology is associated with NREM-sleep ***,more and more results appear to suggest that sleep problems are not only a symptom of AD but can also increase the risk of *** of these studies suggest that it is primarily a lack of NREM-sleep that is responsible for this increased ***,the majority investigated sleep only through subjective reporting,as a result of which NREMsleep could not be analyzed *** aim of this literature study is therefore to present the results of the studies that relate the AD pathology and NREM-sleep(registered by electroencephalography).furthermore,we try to evaluate whether NREM-sleep analysis could be used to support the diagnosis of AD and whether NREM-sleep deficiency could be a causal factor in the development of AD.

关键词： Alzheimer’s disease Mild cognitive impairment Sleep Non-rapid eye movement sleep Amyloid beta-peptides Tau proteins Electroencephalography

Systems Biology of Plant-Microbiome Interactions

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Molecular Plant 2019年第6期12卷 804-821页

作者： Patricia A.Rodriguez Michael Rothballer Soumitra Paul Chowdhury Thomas Nussbaumer Caroline Gutjahr Pascal falter-Braun Institute of Network Biology (INET) Helmholtz Zentrum Munchen German Research Center for Environmental Health Munich Germany Institute of Environmental Medicine (IEM) UNIKA-T Technical University of Munich Augsburg Germany Plant Genetics TUM School of Life Science Weihenstephan Technical University of Munich (TUM) Freising Germany Microbe-Host Interactions Faculty of Biology Ludwig-Maximilians-Universitat (LMU) Mijnchen Munich Germany

In natural environments,plants are exposed to diverse microbiota that they interact with in complex *** plant-pathogen interactions have been intensely studied to understand defense mechanisms in plants,many microbes and microbial communities can have substantial beneficial effects on their plant *** beneficial effects include improved acquisition of nutrients,accelerated growth,resilience against pathogens,and improved resistance against abiotic stress conditions such as heat,drought,and ***,the beneficial effects of bacterial strains or consortia on their host are often cultivar and species specific,posing an obstacle to their general ***,many of the signals that trigger plant immune responses are molecularly highly similar and often identical in pathogenic and beneficial ***,it is unclear what determines the outcome of a particular microbe-host interaction and which factors enable plants to distinguish beneficials from *** unravel the complex network of genetic,microbial,and metabolic interactions,including the signaling events mediating microbe-host interactions,comprehensive quantitative systems biology approaches will be needed.

关键词： plant systems biology plant microbiome microbial communities SynComs microbe-host interactions

iPlant Systems Biology (iPSB): An International Network Hub in the Plant Community

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Molecular Plant 2019年第6期12卷 727-730页

作者： Pascal falter-Braun Siobhan Brady Rodrigo A.Gutierrez Gloria M.Coruzzi Gabriel Krouk Institute of Network Biology (INET) Helmholtz Zentrum Munchen (HMGU) 85764 Munchen-Neuherberg Germany Microbe-Host Interactions Faculty of Biology. Ludwig-Maximilians-University (LMU) Munich 82152 Planegg-Martinsried Germany Department of Plant Biology and the Genome Center University of California Davis CA 95616 USA Departamento de Genetica Molecular y Microbiologia FONDAP Center for Genome Regulation Millennium Institute for Integrative Biology Pontificia Universidad Catolica de Chile Santiago 8331150 Chile Center for Genomics and Systems Biology Department of Biology New York University New York NY 10003 USA B&PMP Univ Montpellier CNRS INRA SupAgro Montpellier France

sequenced genome of a multicellular organism (Arabidopsis Genome, 2000) completed just after those of the nematode Caenorhabditis elegans (C. elegans Sequencing Consortium, 1998) and the fruit fly Drosophila melanogaster (Adams et al., 2000). Availability of a full whole-genome sequence for the reference plant opened to plant biologists what is commonly called the post-genomic era.

关键词： Systems Biology International Network Hub Plant Community

新型冠状病毒肺炎大流行对英国心血管病服务的间接影响

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中华高血压杂志 2021年第1期29卷 61-61页

作者：刘青练桂丽 Ball S Banerjee A Berry C Boyle JR Bray B Bradlow W Chaudhry A Crawley R Danesh J Denniston A falter f figueroa JD Hall C Hemingway H Jefferson E Johnson T King G Lee KK McKean P Mason S Mills NL Pearson E Pirmohamed M Poon MTC Priedon R Shah A Sofat R Sterne JAC Strachan fE Sudlow CLM Szarka Z Whiteley W Wyatt M CVD-COVID-UK Consortium

监测新型冠状病毒肺炎(coronavirus disease,COVID)-19大流行期间医院心血管病的表现、诊断和治,以了解COVID-19的间接影响。方法:采用2019年10月28日(COVID-19之前)至2020年5月10日(解除禁闭前)以及2018-2019年同一时间的医院活动数据,... 详细信息

监测新型冠状病毒肺炎(coronavirus disease,COVID)-19大流行期间医院心血管病的表现、诊断和治,以了解COVID-19的间接影响。方法:采用2019年10月28日(COVID-19之前)至2020年5月10日(解除禁闭前)以及2018-2019年同一时间的医院活动数据,对9家英国医院进行回顾性系列横断面研究。研究者分析了流行前和流行期间选定的心血管病的总体数据,并开发了一个在线可视化工具,可以近乎实时地监测变化趋势。结果:在9家医院中,禁闭后(2020年3月23日)的总住院人数和急诊科就诊人数较上年分别下降57.9%(57.1%~58.6%)和52.9%(52.2%~53.5%)。

关键词：就诊人数住院人数心血管病新型冠状病毒急诊科横断面研究肺炎流行期间

Unusual ovarian hyperstimulation syndrome presentation:Pleural effusion without ascites.A case report

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World Journal of Clinical Cases 2025年第8期13卷 26-34页

作者： Íngrid Solsona Sara Peralta Yasmina Barral francisco fàbregues Pepita Giménez-Bonafé Department of Physiological Sciences Physiology UnitFaculty of Medicine and Health SciencesBellvitge CampusUniversitat de BarcelonaIDIBELLBarcelona 08907CataloniaSpain Institut Clinic of Gynecology Obstetrics and NeonatologyHospital ClínicInstitut d'Investigacions Biomèdiques August Pi i SunyerFaculty of MedicineUniversity of BarcelonaBarcelona 08036CataloniaSpain Department of Physiological Sciences Faculty of Medicine and Health SciencesBellvitge CampusUniversitat de BarcelonaIDIBELLL'Hospitalet de Llobregat 08907BarcelonaSpain

BACKGROUND Ovarian hyperstimulation syndrome(OHSS)is a life-threatening complication that can occur in the luteal phase or early pregnancy after controlled ovarian *** case report highlights a unique manifestation of OHSS involving pleural effusion(PE)in a patient without identifiable risk *** SUMMARY A 39-year-old woman who underwent controlled ovarian hyperstimulation for an in vitro fertilization(IVf)cycle experienced dyspnea on the eleventh day of post oocyte *** diagnosis was severe OHSS with a unique manifestation of PE without *** management involved fluid balance and treatment with albumin,furosemide,thromboembolic prophylaxis,and thoracentesis.A continued drainage of the pleural cavity was *** patient had a favo-rable outcome,and a dichorionic diamniotic gestation passed without *** OHSS and its potential complications can include respiratory distress and PE,as well as thromboembolic disorders.

关键词： Dyspnea Infertility In vitro fertilization Ovarian hyperstimulation syndrome Pleural effusion Thoracentesis Case report

Ripple and Uniformity Measurement of a Phased-Array Testing-Machine for round-Bar Testing

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Ripple and Uniformity Measurement of a Phased-Array Testing-...

Gut virome:New key players in the pathogenesis of inflammatory bowel disease

17th World Conference on Non-Destructive Testing

作者： Stephan falter Josef MAIER Ugur TEZCAN GE Inspection Technologies GmbH

＜正＞Industrial testing of round bar material is commonly carried out directly in the testing line applying automated *** the simple handling possibilities and the excellent resolution,phased-array systems,preferably of type ROWA are applied for these *** to now,many special materials with associated non-standard specifications are still tested offline in immersion tanks or even *** addition to the small reference defects applied,the testing of those materials requires am in depth understanding of the variation of the defect signal along the complete circumference of the test body,generally referred to as Ripple and *** the process point of view,it is of great advantage to transfer the offline test of special machines to the inline testing *** order to do this,a detailed measurement of the testing homogeneity under industrial conditions is one of the key *** this purpose,a method and a measurement system,which allows measuring Ripple and Uniformity,was developed and set into operation.

关键词： Phased-array ROWA Bar testing Ripple & uniformity

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World Journal of Methodology 2025年第2期15卷 27-43页

作者： Helal f Hetta Rehab Ahmed Yasmin N Ramadan Hayam fathy Mohammed Khorshid Mohamed M Mabrouk Mai Hashem Department of Medical Microbiology and Immunology Faculty of MedicineAssiut UniversityAssiut 71515Egypt Division of Microbiology Immunology and BiotechnologyFaculty of pharmacyUniversity of TabukTabuk 71491Saudi Arabia Division of Microbiology Immunology and BiotechnologyDepartment of Natural Products and Alternative MedicineFaculty of PharmacyUniversity of TabukTabuk 71491Saudi Arabia Department of Internal Medicine Division HepatogastroenterologyAssiut UniversityAssiut 71515Egypt Department of Clinical Research Egyptian Developers of Gastroenterology and Endoscopy FoundationCairo 11936Egypt Department of Internal Medicine Faculty of Medicine.Tanta UniversityTanta 31527Egypt Department of Tropical Medicine Gastroenterology and HepatologyAssiut University HospitalAssiut 71515Egypt

Inflammatory bowel disease(IBD)is a chronic inflammatory illness of the *** the mechanism underlying the pathogenesis of IBD is not fully under-stood,it is believed that a complex combination of host immunological response,environmental exposure,particularly the gut microbiota,and genetic suscept-ibility represents the major *** gut virome is a group of viruses found in great frequency in the gastrointestinal tract of *** gut virome varies greatly among individuals and is influenced by factors including lifestyle,diet,health and disease conditions,geography,and *** majority of research has focused on the significance of gut bacteria in the progression of IBD,although viral populations represent an important component of the *** conducted this review to highlight the viral communities in the gut and their expected roles in the etiopathogenesis of IBD regarding published research to date.

关键词： Inflammatory bowel disease Pathogenesis Gut virome Bacteriophage Eukaryotic viruses

Complement-dependent neuroinflammation in spinal cord injury:from pathology to therapeutic implications

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Neural Regeneration Research 2025年第5期20卷 1324-1335页

作者： Hassan Saad Bachar El Baba Ali Tfaily firas Kobeissy Juanmarco Gutierrez Gonzalez Daniel Refai Gerald R.Rodts Christian Mustroph David Gimbel Jonathan Grossberg Daniel L.Barrow Matthew f.Gary Ali M.Alawieh Department of Neurosurgery Emory University School of MedicineAtlantaGAUSA Department of Neurology Yale UniversityNew HavenCTUSA Center for Neurotrauma Multiomics&Biomarkers(CNMB)Morehouse School of MedicineAtlantaGAUSA

Spinal cord injury remains a major cause of disability in young adults,and beyond acute decompression and rehabilitation,there are no pharmacological treatments to limit the progression of injury and optimize recovery in this *** the thorough investigation of the complement system in triggering and propagating cerebral neuroinflammation,a similar role for complement in spinal neuroinflammation is a focus of ongoing *** this work,we survey the current literature investigating the role of complement in spinal cord injury including the sources of complement proteins,triggers of complement activation,and role of effector functions in the *** study relevant data demonstrating the different triggers of complement activation after spinal cord injury including direct binding to cellular debris,and or activation via antibody binding to damage-associated molecular *** effector functions of complement have been implicated in spinal cord injury,and we critically evaluate recent studies on the dual role of complement anaphylatoxins in spinal cord injury while emphasizing the lack of pathophysiological understanding of the role of opsonins in spinal cord *** this pathophysiological review,we systematically review the different translational approaches used in preclinical models of spinal cord injury and discuss the challenges for future translation into human *** review emphasizes the need for future studies to dissect the roles of different complement pathways in the pathology of spinal cord injury,to evaluate the phases of involvement of opsonins and anaphylatoxins,and to study the role of complement in white matter degeneration and regeneration using translational strategies to supplement genetic models.

关键词： complement neuroinflammation neuroplasticity regeneration spinal cord injury targeted therapy