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Charcot-Marie-Tooth-1A and sciatic nerve crush rat models:insights from proteomics

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Neural Regeneration Research 2023年第6期18卷 1354-1363页

作者： Zeina Msheik Stephanie Durand Emilie Pinault Martial Caillaud Laetitia Vignaud fabrice Billet Mohamed El Massry Alexis Desmoulière UR20218 NeurIT(NEURopathies périphériques et Innovation Thérapeutique) University of LimogesLimogesFrance BISCEm(Biologie Intégrative SantéChimie Environnement)Platform US 42 Inserm/UAR 2015 CNRSUniversity of LimogesLimogesFrance UMR 1308 Inserm/CHU–CAPTuR(Contrôle de l’Activation cellulaire Progression Tumorale et Résistance thérapeutique)University of LimogesLimogesFrance Inserm UMR1235–TENS(The Enteric Nervous System in Gut and Brain Diseases) University of NantesNantesFrance

The sensorimotor and histological aspects of peripheral neuropathies were already studied by our team in two rat models:the sciatic nerve crush and the Charcot-Marie-Tooth-1A *** this study,we sought to highlight and compare the protein signature of these two pathological ***,the identification of protein profiles in diseases can play an important role in the development of pharmacological *** fact,Charcot-Marie-Tooth-1A rats develop motor impairments that are more severe in the hind ***,for the first time,protein expression in sciatic nerve of Charcot-Marie-Tooth-1A rats was ***,distal sciatic nerves were collected from Charcot-Marie-Tooth-1A and uninjured wild-type rats aged 3 *** protein extraction,sequential window acquisition of all theoretical fragment ion spectra liquid chromatography and mass spectrometry was employed.445 proteins mapped to Swiss-Prot or trEMBL Uniprot databases were identified and *** these,153 proteins showed statistically significant differences between Charcot-Marie-Tooth-1A and wild-type *** majority of these proteins were overexpressed in *** clustering and functional enrichment using Gene Ontology were used to group these proteins based on their biological effects concerning Charcot-Marie-Tooth-1A ***,proteomic characterization of wild-type rats subjected to sciatic nerve crush was performed sequential window acquisition of all theoretical fragment ion spectra liquid chromatography and mass *** month after injury,distal sciatic nerves were collected and analyzed as described *** of 459 identified proteins,92 showed significant differences between sciatic nerve crush and the uninjured wild-type rats used in the first *** results suggest that young adult Charcot-Marie-Tooth-1A rats(3 months old)develop compensatory mechanisms at the level of redox balance,protein folding,myelination,and

关键词： Charcot-Marie-Tooth-1A endoplasmic reticulum Gene Ontology neurogenesis oxidative stress proteomics rat repair sciatic nerve crush SWATH-MS

Dissipation of chlorpyrifos, pendimethalin, and thiophanate-methyl pesticides in compost based-versus peat-moss based-biomixture of biobeds

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Waste Disposal and Sustainable Energy 2023年第4期5卷 461-470页

作者： Talaat N.M.El-Sebai Wafaa M.H.Zidan Alaa Marzouk Marion Divers fabrice Martin-Laurent Agricultural Microbiology Dept.Agricultural and Biology Research Institute National Research CentreCairoEgypt Laboratory of Pesticide Analysis Agriculture Research CentreCairoEgypt Laboratory of Soil Microbiology National Institute of Agricultural ResearchDijonFrance

Biobed is a smart bioremediation system used to treat point-source pesticide *** is the main component of biobeds,and pesticide dissipation is affected by its *** study aimed to compare the effectiveness of compost-based(C)versus peat-moss-based(P)biomixtures of biobeds on tested pesticide *** concentrations(25,50,and 75 mg/kg)of chlorpyrifos,pendimethalin,and thiophanate methyl were added separately and as a mix to both biomixtures C and *** data showed the effect of biomixture type on the dissipation of the tested *** chlorpyrifos,its dissipation rate in biomixture P was more rapid than that in biomixture *** result was confirmed by the mineralization kinetic experiment,since 25%of the initial ^(14)C-chlorpyrifos concentration accumulated in the form ^(14)CO_(2) in biomixture P compared to only 14%in biomixture *** addition,the chlorpyrifos dissipation rate was influenced by the initial concentration when applied individually in biomixture *** contrast,biomixture C was more effective at pendimethalin dissipation than biomixture P,since>76%of pendimethalin was dissipated in biomixture C versus 67%in biomixture P at the same incubation *** thiophanate-methyl,the abilities of both biomixtures C and P were similar and less efficient than those of the other tested *** addition of the three tested pesticides to biomixture C only had a positive effect on both chlorpyrifos and thiophanate-methyl dissipation,while pendimethalin dissipation was similar when applied separately or as a *** activity was stimulated by the addition of separately or mixed pesticides to biomixtures C and P as measured by dehydrogenase activity.

关键词： Biopurification system Pesticides Contamination Point-source Bioremediation Biomixture

Biscuits: a systematic review and meta-analysis of improving the nutritional quality and health benefits

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Food Production, Processing and Nutrition 2021年第1期3卷 335-352页

作者： MahamadéGoubgou Laurencia TSongré-Ouattara fabrice Bationo Hagrétou Lingani-Sawadogo Yves Traoré Aly Savadogo Doctoral School of Sciences and Technology Laboratory of Applied Biochemistry and Immunology(LABIA)University Joseph KI-ZERBOOuagadougouBurkina Faso Department of Food Technology(DTA) Research Institute of Applied Sciences and Technology(IRSAT)OuagadougouBurkina Faso

Biscuits are ready-to-eat foods that are traditionally prepared mainly with wheat flour,fat,and ***,biscuits’technologies have been rapidly developed to improve their nutritional *** study aimed to determine the strategies of improving the nutritional quality of biscuits and the potential health benefits associated with them.A systematic review and meta-analysis were conducted,including articles on biscuits improved by technological processes and raw materials *** were searched from Google Scholar,PubMed,Scopus,and Web of Science published between 1997 and 2020,in English and *** meta-analysis was performed using RStudio software,version 4.0.4 to classify the *** hundred and seven eligible articles were ***,pea,potato,sorghum,buckwheat,and flaxseed flours were respectively the most found substitutes to wheat *** the meta-analysis shown that the copra and foxtail millet biscuit fortified with amaranth,the wheat biscuits fortified with okra,and rice biscuits fortified with soybeans had a high protein *** biscuits therefore have a potential to be used as complementary *** substitution of sugar and fat by several substitutes lead to a decrease in carbohydrates,fat,and energy *** has also brought about an increase in other nutrients such as dietary fiber,proteins/amino acids,fatty acids,and phenolic *** the sugar and fat substitutes,stevia and inulin were respectively the most *** the use of biscuits in clinical trials,they were mainly used for addressing micronutrient deficiency and for weight loss.

关键词： Biscuit Technology Nutrition Health benefits

Testing for hepatitis B virus alone does not increase vaccine coverage in non-immunized persons

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World Journal of Gastroenterology 2017年第38期23卷 7037-7046页

作者： Anders Boyd Julie Bottero fabrice Carrat Joel Gozlan Hayette Rougier Pierre-Marie Girard Karine Lacombe Institute Pierre Louis d’épidémiologie et de SantéPublique(IPLESP UMRS 1136) INSERMSorbonne Universités Service de maladies infectieuses Hopital Saint-AntoineAP-HP Département de santépublique Hopital Saint-AntoineAP-HP Laboratoire de Virologie Hopital Saint-AntoineAP-HP INSERM UMR_S1135 CIMI

AIM To determine whether hepatitis B virus(HBV)-testing could serve as a gateway to vaccinate non-immunized individuals in a low-prevalent *** Non-immunized subjects participating in a multi-center, HBV-testing campaign in Paris, France were identified and contacted via telephone 3-9 mo after testing in order to determine vaccination status. Vaccination coverage was evaluated in per-protocol(for all respondents) and intent-to-treat analysis(assuming all non-responders did not vaccinate).RESULTS In total, 1215/4924(24.7%) enrolled subjects with complete HBV serology were identified as nonimmunized and eligible for analysis. There were 99/902 successfully contacted subjects who had initiated HBV vaccination after screening: per-protocol, 11.0%(95%CI: 9.0-13.2); intent-to-treat, 8.2%(95%CI: 6.7-9.8). In multivariable analysis, vaccination was more likely to be initiated in individuals originating from moderate or high HBV-endemic countries(P < 0.001), patients with limited healthcare coverage(P = 0.01) and men who have sex with men(P = 0.02). When asked about the reasons for not initiating HBV vaccination, the most frequent response was "will be vaccinated later"(33.4%), followed by "did not want to vaccinate"(29.8%), and "vaccination was not proposed by the physician"(21.5%). Sub-group analysis indicated a stark contrast in vaccination coverage across centers, ranging from 0%-56%.CONCLUSION HBV-vaccination after HBV screening was very low in this study, which appeared largely attributed to physician-patient motivation towards vaccination. Increased vaccination coverage might be achieved by emphasizing its need at the organizational level.

关键词： Health service organization Hepatitis B virus vaccination Public health Testing intervention Vaccine coverage

A de novo germline MLH1 mutation in a Lynch syndrome patient with discordant immunohistochemical and molecular biology test results

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World Journal of Gastroenterology 2012年第39期18卷 5635-5639页

作者： fabrice Airaud Sébastien Küry Isabelle Valo Ingrid Maury Dominique Bonneau Olivier Ingster Stéphane Bezieau CHU Nantes Service de Génétique Médicale9 quai Moncousu44093 Nantes Cedex 1France Institut de Cancérologie de l'Ouest Service d'Anatomie et Cytologie Pathologique2 rue Moll49933 Angers Cedex 9France CHU Angers Service de Génétique Médicale4 rue Larrey49933 AngersFrance

We describe a patient with a Homo sapiens mutL homolog 1 (MLH1)-associated Lynch syndrome with previous diagnoses of two distinct primary cancers:a sigmoid colon cancer at the age of 39 years, and a right colon cancer at the age of 50 years. The mutation identified in his blood and buccal cells, c.1771delG, ***591Ilefs*25, appears to be a de novo event, as it was not transmitted by either of his parents. This type of de novo event is rare in MLH1 as only three cases have been reported in the literature so far. Further-more, the discordant results observed between repli-cation error phenotyping and immunohistochemistry highlight the importance of the systematic use of both pre-screening tests in the molecular diagnosis of Lynch syndrome.

关键词： Lynch syndrome Homo sapiens mutL ho-molog 1 De novo mutation Replication error pheno- type Immunohistochemistry

Cerebellar pathology in motor neuron disease:neuroplasticity and neurodegeneration

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Neural Regeneration Research 2022年第11期17卷 2335-2341页

作者： Rangariroyashe H.Chipika Grainne Mulkerrin Pierre-François Pradat Aizuri Murad fabrice Ango Cédric Raoul Peter Bede Computational Neuroimaging Group Biomedical Sciences InstituteTrinity College DublinDublinIreland Pitié-Salpêtrière University Hospital Sorbonne UniversityParisFrance The Neuroscience Institute of Montpellier(INM) INSERMCNRSMontpellierFrance

Amyotrophic lateral sclerosis is a relentlessly progressive multi-system *** clinical picture is dominated by upper and lower motor neuron degeneration,but extra-motor pathology is increasingly recognized,including cerebellar ***-mortem and neuroimaging studies primarily focus on the chara cterization of supratentorial disease,des pite emerging evidence of cerebellar degeneration in amyotrophic lateral *** clinical features of amyotrophic lateral sclerosis,such as dysarthria,dysphagia,cognitive and behavioral deficits,saccade abnormalities,gait impairment,respiratory weakness and pseudobulbar affect are likely to be exacerbated by co-existing cerebellar *** review summarizes in vivo and post mortem evidence for cerebellar degeneration in amyotrophic lateral scle *** imaging studies consistently capture cerebellar grey matter volume reductions,diffusivity studies readily detect both intra-cerebellar and cerebellar peduncle white matter alte rations and functional imaging studies commonly report increased functional connectivity with supratentorial *** functional connectivity is commonly interpreted as evidence of neuro plasticity representing compensatory processes despite the lack of post-mortem *** is a scarcity of post-mortem studies focusing on cerebellar alte rations,but these detect pTDP-43 in cerebellar *** rebellar pathology is an overloo ked facet of neurodegeneration in amyotrophic lateral sclerosis despite its contribution to a multitude of clinical symptoms,wides p read connectivity to spinal and supratentorial regions and putative role in compensating for the degeneration of primary motor regions.

关键词： amyotrophic lateral sclerosis ataxia cerebellum magnetic resonance imaging motor neuron disease neuroimaging neuroplasticity pathology primary lateral sclerosis pseudobulbar affect

Systematics and Aquaculture： What Could They Bring to Each Other？

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Journal of Life Sciences 2016年第5期10卷 240-244页

作者： fabrice Teletchea University of Lorraine Vandoeuvre-les-Nancy F-54505 France

Systematics is traditionally based on morphological characters to both define species and establish classifications. In the past decades, partly due to the advent of molecular biology, traditional systematics has declined while molecular systematics has tremendously increased. This results in that fewer funding are generally provided to traditional systematics, particularly for searching new morphological characters. Aquaculture, the farming of aquatic animals, has increased exponentially in the past decades, providing today more than half of fish consumed worldwide, and is expected to continue to rise. Aquaculture requires controlling the life cycle of the farmed fish in captivity, including the rearing of early life stages. Therefore, by coupling systematics and aquaculture, it could be possible to bring new funds and facilities to the former to study the early life stages of numerous fish species and to the latter it would offer a conceptual framework to perform comparative ontogeny. Together, this could help improving our knowledge on the early life stages that could be useful for both taxonomists and zootechnicians.

关键词： Systematics taxonomy aquaculture early life stages fish.

Formulation, stability testing, and analytical characterization of melatonin-based preparation for clinical trial

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Journal of Pharmaceutical Analysis 2017年第4期7卷 237-243页

作者： Samira Filali Charlotte Bergamelli Mamadou Lamine Tall Damien Salmon Diane Laleye Carole Dhelens Elhadji Diouf Christine Pivot fabrice Pirot Service pharmaceutique Plateforme FRIPHARMGroupement Hospitalier Edouard Herriot5 Place d′ArsonvalF-69437 Lyon Cedex 03France Laboratoire de Recherche et Développement de Pharmacie Galénique Industrielle Plateforme FRIPHARMFaculté de PharmacieLaboratoire de Biologie Tissulaire et Ingénierie Thérapeutique – UMR 5305Université Claude Bernard Lyon 18 Avenue RockefellerF-69373 Lyon Cedex 08France

A new institutional clinical trial assessed the improvement of sleep disorders in 40 children with autism treated by immediate-release melatonin formulation in different regimens(0.5 mg, 2 mg, and 6 mg daily) for one month. The objectives of present study were to(i) prepare low-dose melatonin hard capsules for pediatric use controlled by two complementary methods and(ii) carry out a stability study in order to determine a use-bydate. Validation of preparation process was claimed as ascertained by mass uniformity of hard *** analysis by attenuated total reflectance Fourier transformed infrared(ATR-FTIR) of melatonin/microcrystalline cellulose mixture allowed to identify and quantify relative content of active pharmaceutical ingredients and excipients. Absolute melatonin content analysis by high performance liquid chromatography in 0.5 mg and 6 mg melatonin capsules was 93.6% ± 4.1% and 98.7% ± 6.9% of theoretical value, respectively. Forced degradation study showed a good separation of melatonin and its degradation products. The capability of the method was 15, confirming a risk of false negative < 0.01%. Stability test and dissolution test were compliant over 18 months of storage with European Pharmacopoeia. Preparation of melatonin hard capsules was completed manually and melatonin in hard capsules was stable for 18 months, in spite of low doses of active ingredient. ATR-FTIR offers a real alternative to HPLC for quality control of highdose melatonin hard capsules before the release of clinical batches.

关键词： Melatonin Stability-indicating method High-performance liquid chromatography Multicomponent infrared analysis Clinical trial Autism

Identification and expression analysis of the Pbr MLO gene family in pear, and functional verification of PbrMLO23

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Journal of Integrative Agriculture 2021年第9期20卷 2410-2423页

作者： GUO Bing-bing LI Jia-ming LIU Xing QIAO Xin Musana Rwalinda fabrice WANG Peng ZHANG Shao-ling WU Ju-you Center of Pear Engineering Technology Research/State Key Laboratory of Crop Genetics and Germplasm Enhancement College of HorticultureNanjing Agricultural UniversityNanjing 210095P.R.China School of Life Sciences Nanjing UniversityNanjing 210023P.R.China

Mildew resistance locus O(MLO) is a plant-specific gene family that plays an important role in the growth and development of plants and their interactions with the environment. However, the available information on this gene family in pear is limited. Here, 24 PbrMLO genes were identified and divided into five subfamilies(I, II, III, IV and V). Wholegenome duplication(WGD) and dispersed duplication contributed to the expansion of the PbrMLO family. In addition, gene expression analysis revealed that PbrMLO genes were distributed in various pear tissues, suggesting their diverse functions. We selected PbrMLO23 for further functional analysis. Expression profile analysis by qRT-PCR showed that PbrMLO23 was highly expressed in pollen. Subcellular localization analysis showed that PbrMLO23 was located on the plasma membrane. When the expression level of PbrMLO23 was knocked down by using antisense oligonucleotides, pollen tube lengths increased, indicating that PbrMLO23 plays a functional role in inhibiting pollen tube growth. In summary, these results provide evolutionary insight into PbrMLO and its functional characteristics and lay a foundation for further analysis of the functions of PbrMLO members in pear.

关键词： MLO pear gene expression pollen tube