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Bioactive Materials 2020年第2期5卷 228-232页

作者： Isadora Martini Garcia Stéfani Becker Rodrigues Maria Eduarda Rodrigues Gama Vicente Castelo Branco Leitune Mary Anne Melo fabrício mezzomo collares Dental Materials Laboratory Postgraduate Program in DentistrySchool of DentistryFederal University of Rio Grande Do SulRua Ramiro Barcelos2492Rio Branco90035-003Porto AlegreRSBrazil Ph.D.Program in Biomedical Sciences University of Maryland School of DentistryBaltimoreMD21201USA Operative Dentistry Division General Dentistry Department University of Maryland School of DentistryBaltimoreMD21201USA

To reduce the burden of denture stomatitis and oral candidiasis,an aqueous solution containing polyhexamethylene guanidine hydrochloride(PHMGH)was investigated as an antifungal disinfectant against the leading cause of these oral conditions,Candida *** solutions formulated with concentrations ranging from 0.125 to 0.50 wt%enabled increasing disinfection at the initial 5min-contact with 72h-mature candida biofilms formed on denture liner *** 10 min-contact,the solution at lower concentration has reached total fungal *** results also indicated that the denture liners preserved their mechanical property after the maximum contact time with the solution at the highest tested *** PHMGH aqueous solutions at 0.125 wt%could be applied to promote interim denture liner disinfection without promoting the loss of materials’mechanical property.

关键词： Dental materials Guanidine Antifungal agents Candidiasis Denture liner

Ionic liquid-loaded microcapsules doped into dental resin infiltrants

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Bioactive Materials 2021年第9期6卷 2667-2675页

作者： Marla Cuppini Isadora Martini Garcia Virgínia Serra de Souza Kelly Cristine Zatta Fernanda Visioli Vicente Castelo Branco Leitune Sílvia Stanisçuazki Guterres Jackson Damiani Scholten fabrício mezzomo collares Department of Dental Materials School of DentistryFederal University of Rio Grande do SulRamiro Barcelos Street2492Rio Branco90035-003Porto AlegreRSBrazil Laboratory of Molecular Catalysis Institute of ChemistryFederal University of Rio Grande do SulBento Gonçalves Avenue9500Agronomia91501-970Porto AlegreRSBrazil Cosmetology and Pharmaceutical Nanotechnology Laboratory School of Pharmaceutical SciencesUniversidade Federal do Rio Grande do SulAv.Ipiranga 275290610-000Porto AlegreRSBrazil Oral Pathology Department School of DentistryFederal University of Rio Grande do SulRua Ramiro Barcelos2492Rio Branco90035-003Porto AlegreRSBrazil Cosmetology Laboratory School of Pharmaceutical SciencesUniversidade Federal do Rio Grande do SulAv.Ipiranga 275290610-000Porto AlegreRSBrazil

Resin infiltrants have been effectively applied in dentistry to manage non-cavitated carious lesions in proximal dental ***,the common formulations are composed of inert methacrylate *** this study,we developed a novel resin infiltrant with microcapsules loaded with an ionic liquid(MC-IL),and analyzed the physical properties and cytotoxicity of the dental ***,the ionic liquid 1-n-butyl-3-methylimidazolium bis(trifluoromethanesulfonyl)imide(***2)was ***2 has previously shown antibacterial activity in a dental ***,MC-IL were synthesized by the deposition of a preformed *** MC-IL were analyzed for particle size and de-agglomeration effect via laser diffraction analysis and shape via scanning electron microscopy(SEM).The infiltrants were formulated,and the MC-IL were incorporated at 2.5%,5%,and 10 wt%.A group without MC-IL was used as a *** infiltrants were evaluated for ultimate tensile strength(UTS),contact angle,surface free energy(SFE),and *** MC-IL showed a mean particle size of 1.64(±0.08)μm,shriveled aspect,and a de-agglomeration profile suggestive of nanoparticles’presence in the synthesized *** were no differences in UTS among groups(p>0.05).The incorporation of 10 wt%of MC-IL increased the contact angle(p0.05).The incorporation of microcapsules as a drug-delivery system for ionic liquids may be a promising strategy to improve dental restorative materials.

关键词： Surface properties Dental caries Stress Mechanical Drug delivery systems Polymers

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如何利用历史数据更好地做好投资决策

中华纸业 2024年第3期45卷 19-20页

作者： ResourceWise Marcello collares ResourceWise

对于各大造纸企业来说,具有战略性的决定何时何地投资的能力对于业务增长和保持竞争优势至关重要,而利用好历史数据可以作为解决这一关键问题的指导力量。历史数据提供了对过去趋势、市场行为和客户偏好的宝贵见解。这些信息使企业能够... 详细信息

对于各大造纸企业来说,具有战略性的决定何时何地投资的能力对于业务增长和保持竞争优势至关重要,而利用好历史数据可以作为解决这一关键问题的指导力量。历史数据提供了对过去趋势、市场行为和客户偏好的宝贵见解。这些信息使企业能够根据具体数据做出明智的决策。

关键词：投资决策历史数据造纸企业客户偏好保持竞争优势关键问题战略性

Optimization of enzymatic hydrolysis of cassava to obtain fermentable sugars

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2012年第7期13卷 579-586页

作者： Renata M.collares Luiza V.S.MIKLASEVICIUS Mariana M.BASSACO Nina P.G.SALAU Marcio A.MAZUTTI Dilson A.BISOGNIN Lisiane M.TERRA Department of Chemical Engineering Federal University of Santa MariaAv.Roraima 1000Santa Maria 97105-900Brazil Department of Horticulture Federal University of Santa MariaAv.Roraima 1000Santa Maria 97105-900Brazil

This work evaluates the enzymatic hydrolysis of starch from cassava using pectinase,α-amylase,and amyloglucosidase.A central composite rotational design(CCRD)was carried out to evaluate the effects of amyloglucosidase,pectinase,reaction time,and solid to liquid *** the experiments were carried out in a bioreactor with working volume of 2 *** 98%efficiency hydrolysis was obtained,resulting in a concentration of total reducing sugar released of 160 g/*** was concluded that pectinase improved the hydrolysis of starch from *** time was found to be significant until 7 h of reaction.A solid to liquid ratio of 1.0 was considered suitable for hydrolysis of starch from *** was a significant variable in the process:after its addition to the reaction media,a 30%-50%increase in the amount of total reducing sugar released was *** optimal conditions the maximum productivity obtained was 22.9 g/(L.h).

关键词： Cassava Enzymatic hydrolysis Enzyme reactor Starch

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拉丁美洲商品浆市场展望

中华纸业 2023年第21期44卷 42-43页

作者： Marcello collares 不详

拉丁美洲已成为全球纸浆市场的重要推动力量,该地区多个国家在制浆领域发挥着举足轻重的作用。制浆行业的蓬勃发展也为拉丁美洲带来了显著的经济增长,为创造就业机会、增加出口收入和大量投资配套基础设施铺平了道路。近期,拉丁美洲投... 详细信息

拉丁美洲已成为全球纸浆市场的重要推动力量,该地区多个国家在制浆领域发挥着举足轻重的作用。制浆行业的蓬勃发展也为拉丁美洲带来了显著的经济增长,为创造就业机会、增加出口收入和大量投资配套基础设施铺平了道路。近期,拉丁美洲投资了不少新项目,在不久的将来,或许会有一波新建制浆项目落地的高潮,将在全球市场产生连锁效应。

关键词：创造就业机会连锁效应配套基础设施拉丁美洲项目落地推动力量制浆商品浆市场

Clinical and environmental isolates of Burkholderia pseudomallei from Brazil: Genotyping and detection of virulence gene

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Asian Pacific Journal of Tropical Medicine 2017年第10期10卷 945-951页

作者： Tereza de Jesus Pinheiro Gomes Bandeira Debora de Souza collares Maia Castelo-Branco Marcos Fabio Gadelha Rocha Rossana de Aguiar Cordeiro Crister Jose Ocadaque Manoel de Araújo Neto Paiva Raimunda Samia Nogueira Brilhante Jose Júlio Costa Sidrim Specialized Medical Mycology Center Postgraduate Program in Medical Microbiology Federal University of Ceará Christus School of Medicine Postgraduate Program in Veterinary Sciences State University of Ceará Federal Institute of Education Science and Technology of Ceará

Objective: To evaluate the genetic diversity of clinical and environmental isolates of Burkholderia pseudomallei(***) recovered in Ceara, Brazil, and screen these isolates for the presence of type three secretion system virulence ***: Nineteen *** isolates(9 from clinical cases and 10 from soils)were *** amplified polymorphic DNA was performed with primers OPQ-2, OPQ-4 and OPQ-16 to evaluate the genetic diversity, and type three secretion system gene was detected through polymerase chain ***: Random amplified polymorphic DNA showed a genetic relatedness of approximately 50% among the tested *** isolates, which were grouped into two clades, of which the biggest ones comprised 18/19 isolates for primer OPQ-2, and 17/19 isolates for primer *** OPQ-4 grouped the isolates into three clades comprising 1/19, 3/19 and 15/19 ***, type three secretion system gene was detected in all tested ***: This is an effort to type *** strains from Ceara, which is important for better understanding this pathogen, contributing for the epidemiological surveillance of melioidosis in this endemic region.

关键词： Burkholderia pseudomallei Melioidosis RAPD TTSS gene

Biofilm analyses and exoproduct release by clinical and environmental isolates of Burkholderia pseudomallei from Brazil

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Asian Pacific Journal of Tropical Medicine 2023年第7期16卷 321-328页

作者： Glaucia Morgana de Melo Guedes Crister JoséOcadaque Alyne Soares Freitas Rodrigo Machado Pinheiro Giovanna Barbosa Riello Silviane Praciano Bandeira Rossana de Aguiar Cordeiro Marcos Fábio Gadelha Rocha JoséJúlio Costa Sidrim Débora de Souza collares Maia Castelo-Branco Department of Pathology and Legal Medicine Postgraduate Program in Medical MicrobiologyGroup of Applied Medical MicrobiologyFederal University of CearáFortalezaCEBrazil Department of Clinical and Toxicological Analysis School of PharmacyFederal University of CearáFortalezaCEBrazil Department of Pathology and Legal Medicine Postgraduate Program in Medical MicrobiologyLaboratory of Emerging and Reemerging PathogensFederal University of CearáFortalezaCEBrazil School of Veterinary Medicine Postgraduate Program in Veterinary SciencesState University of CearáFortalezaCEBrazil

Objective:To characterize biofilm production by clinical(n=21)and environmental(n=11)isolates of Burkholderia pseudomallei and evaluate the production of proteases,hemolysins and ***:Initially,the 32 strains were evaluated for biofilm production in Müller-Hinton broth-1%glucose(MH-1%glucose)and BHI broth-1%glucose,using the crystal violet staining ***,growing(48 h)and mature(72 h)biofilms were evaluated by confocal ***,the production of proteases,hemolysins and siderophores by planktonic aggregates,growing biofilms and mature biofilms was ***:All isolates produced biofilms,but clinical isolates had significantly higher biomass in both MH-1%glucose(P<0.001)and BHI-glucose 1%(P=0.005).The structural analyses by confocal microscopy showed thick biofilms,composed of multiple layers of cells,homogeneously arranged,with mature biofilms of clinical isolates presenting higher biomass(P=0.019)and thickness of the entire area(P=0.029),and lower roughness coefficient(P=0.007)than those of environmental *** production by growing biofilms was significantly greater than that of planktonic(P<0.001)and mature biofilms(P<0.001).Hemolysin release by planktonic aggregates was higher than that of biofilms(P<0.001).Regarding siderophores,mature biofilms presented higher production than growing biofilms(P<0.001)and planktonic aggregates(P<0.001).Conclusions:Clinical isolates have higher production of biofilms than their environmental counterparts;protease and siderophores seem important for growth and maintenance of Burkholderia pseudomallei biofilms.

关键词： Mangosteen Burkholderia pseudomallei Biofilms Proteases Hemolysins Siderophores

Chlamydoconidium-producing Trichophyton tonsurans: Atypical morphological features of strains causing tinea capitis in Ceará, Brazil

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Asian Pacific Journal of Tropical Medicine 2019年第8期12卷 380-384页

作者： Raimunda Samia Nogueira Brilhante Germana Costa Paixao Jonathas Sales de Oliveira Vandbergue Santos Pereira Marcos Fábio Gadelha Rocha Reginaldo Goncalves de Lima-Neto Débora de Souza collares Maia Castelo-Branco Rossana de Aguiar Cordeiro JoséJúlio Costa Sidrim Specialized Medical Mycology Center Postgraduate Program in Medical MicrobiologyFederal University of CearáRua Nunes de Melo1315FortalezaBrazil Postgraduate Program in Veterinary Sciences State University of CearáAv.Dr.Silas Munguba1700FortalezaBrazil Department of Tropical Medicine Center for Health SciencesFederal University of PernambucoRecife-PEBrazil

Objective: To report atypical morphological features of Trichophyton(T.) tonsurans strains associated with tinea capitis. Methods: Eighty-two T. tonsurans strains isolated in Ceará, Brazil, were analyzed regarding macro and micromorphological features and nutritional patterns. Results: Fifty-two samples presented abundant chlamydoconidia, which were produced in chains. Macroscopically, these strains developed small glabrous colonies that were firmly attached to the surface of the culture medium, with few or no aerial mycelia and intense rusty yellow pigmentation. Seven strains did not grow with stimulus from thiamine. Samples were heterogeneous regarding urease production and none presented in vitro hair perforation. Conclusions: The observation of T. tonsurans strains with distinct phenotypic features indicates the need to revise the taxonomic criteria for routine identification of this dermatophyte.

关键词： Trichophyton tonsurans Chlamydoconidia Dermatophyte Phenotyping

Research advances on the multiple uses of Moringa oleifera: a sustainable alternative for socially neglected population

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Asian Pacific Journal of Tropical Medicine 2017年第7期10卷 689-700页

作者： Raimunda Samia Nogueira Brilhante Jamille Alencar Sales Vandbergue Santos Pereira Débora de Souza collares Maia Castelo-Branco Rossana de Aguiar Cordeiro Célia Maria de Souza Sampaio Manoel de Araújo Neto Paiva Joao Bosco Feitosa dos Santos JoséJúlio CostaSidrim Marcos Fábio Gadelha Rocha Department of Pathology and Legal Medicine Postgraduate Program in Medical MicrobiologySpecialized Medical Mycology CenterFederal University of Ceará School of Veterinary Medicine Postgraduate Program in Veterinary SciencesState University of Ceará Department of Social Sciences Postgraduate Program of SociologyState University of Ceará

Moringa oleifera Lam(Moringaceae) is a plant with high nutritional and medicinal value. Native to India, it is now widely distributed throughout tropical and subtropical regions of the world. Its different parts are sources of proteins, vitamins and minerals and present different pharmacological and biotechnological potential. Moreover, M. oleifera seeds are widely used in water and effluent treatment, for their coagulation, flocculation and sedimentation properties, their ability of improving water quality, by reducing organic matter and microbial load, with special applicability in intensive animal production systems, such as aquaculture. In addition, due to its high nutritional value and several medicinal properties, this tree may act as a nutritional and medical alternative for socially neglected populations. In this context, this review gathers information on M. oleifera, emphasizing its chemical constituents, nutritional, pharmacological and antimicrobial properties, applications in the treatment of water effluents, and ecological and social aspects.

关键词： Moringa oleifera Biotechnology Nutrition Health Aquaculture

Vibrio *** Macrobrachium amazonicum prawn farming are inhibited by Moringa oleifera extracts

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Asian Pacific Journal of Tropical Medicine 2015年第11期8卷 899-902页

作者： Raimunda Samia Nogueira Brilhante Jamille Alencar Sales Celia Maria de Souza Sampaio Francisco Geraldo Barbosa Manoel de Araujo Neto Paiva Glaucia Morgana de Melo Guedes Lucas Pereira de Alencar Yago Brito de Ponte Tereza de Jesus Pinheiro Gomes Bandeira Jose Luciano Bezerra Moreira Debora de Souza collares Maia Castelo-Branco Waldemiro de Aquino Pereira-Neto Rossana de Aguiar Cordeiro Jose Julio Costa Sidrim Marcos Fabio Gadelha Rocha Department of Pathology and Legal Medicine Postgraduate Program in Medical MicrobiologySpecialized Medical Mycology CenterFederal University of Ceara School of Veterinary Medicine Postgraduate Program in Veterinary SciencesState University of Ceara Department of Organic and Inorganic Chemistry Federal University of Ceara

Objective:To investigate the in vitro antimicrobial potential of extracts of stem,leaves,flowers,pods and seeds of Moringu okifera(***) against Vibrio *** hatchery water and the prawn Mmrobrachium ***:The ethanol extracts of stem,leaves,pods and seeds and chloroform extract of flowers of *** were tested against Vibrio cholerue(***) serogroups non-O1/non-O139(n=4).Vibrio vulnificus(n=l) and Vibrio mimicus(n=1).Escherichia coli(***)(ATCC(?) 25922) was used as quality *** species were obtained from Macmbrachium amazonicum prawns and from hatchery water from prawn *** Minimum Inhibitory Concentration(MIC) was determined by broth microdilution ***:The best result was obtained with the ethanol extract of pods,which inhibited three strains of the ***,Vibrio vulnificus,Vibrio minimis and ***(MIC range 0.312-5.000 mg/mL).The chloroform extract of flowers was effective against all *** strains and ***(MIC range 0.625-1.250 mg/mL).However,the ethanol extracts of stem and seeds showed low effectiveness in inhibiting the bacterial ***:The extracts of pods,flowers and leaves of *** have potential for the control of Vibrio *** studies are necessary to isolate the bioaetive compounds responsible for this antimicrobial activity.

关键词： Prawn Macrobrachium amazonicum Moringa oleifera Vi