检索结果-南通市图书馆

Streptococcus agalactiae:Identification methods,antimicrobial susceptibility, and resistance genes in pregnant women

引用

World Journal of Clinical Cases 2020年第18期8卷 3988-3998页

作者： fabrícia almeida fernandes santana Tais Viana Ledo de Oliveira Marcelo Barreto de Souza Filho Lucas santana Coelho da Silva Breno Bittencourt de Brito fabrício Freire de Melo Cláudio Lima Souza Lucas Miranda Marques Márcio Vasconcelos Oliveira Instituto Multidisciplinar em Saúde Universidade Federal da BahiaVitória da Conquista 45029-094BahiaBrazil Laboratório Oliveira Ltda LABOVitória da Conquista 45020740BahiaBrazil Campus Soane Nazaréde Andrade Universidade Estadual de Santa CruzIlhéus 45662900BahiaBrazil

BACKGROUND Group B Streptococcus(GBS)is a normal component of the gastrointestinal and genital microbiota in humans and can lead to important infections in *** To compare GBS isolation and identification methods as well as to assess the antibiotic susceptibility and to identify resistance genes in GBS strains from pregnant women attended in healthcare services from the city of Vitória da Conquista,in Bahia State,*** From January 2017 to February 2018,vaginorectal swabs were obtained from 186 participants and the samples were seeded onto chromogenic agar for GBS before and after inoculation in selective *** identification using 3 CAMP and latex tests was performed in samples with GBS-suggestive ***,disk diffusion antibiograms were performed in GBS-positive samples,and the detection of the resistance genes ermB,ermTR,mefA,and linB in the clindamycin and/or erythromycin-resistant samples was carried *** Thirty-two samples(17.2%)were *** culture in chromogenic agar after sample incubation in selective broth was the most sensitive method(96.9%)for GBS *** isolates were susceptible to penicillin,ampicillin,cefotaxime,and *** resistance was observed in 6 samples(18.8%),while 8 samples(25%)were *** erythromycin and/or clindamycin-resistant GBS strains had negative *** strains(25%)presented an M phenotype and 6 isolates(75%)presented a cMLSB *** ermB gene was identified in 4 samples(44.4%),the mefA gene was also found in 4 samples(44.4%),the ermTR gene was identified in 1 isolate(11.1%),and the linB gene was not found in any *** This study evidenced that the screening for SGB can be performed by means of various methods,including chromogenic media,and that the chemoprophylaxis for pregnant women who cannot use penicillin must be susceptibility-guided.

关键词： Streptococcus agalactiae Pregnancy Antimicrobial susceptibility

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Streptococcus agalactiae:Sensitivity profile in pregnant women attending health units in northeastern Brazil

引用

World Journal of Obstetrics and Gynecology 2020年第1期9卷 11-17页

作者： Tais Viana Ledo de Oliveira fabrícia almeida fernandes santana Caline Novais Teixeira Oliveira Maria Luísa Cordeiro Santos fabrício Freire de Melo Cláudio Lima Souza Márcio Vasconcelos Oliveira Instituto Multidisciplinar em Saúde Universidade Federal da BahiaVitória da Conquista 45029-094BahiaBrazil

BACKGROUNDG roup B Streptococcus agalactiae (GBS) is the main etiologic agent associated withearly-onset neonatal sepsis, and of all newborns of parturients colonized by GBS,approximately 1%-2% develop invasive, early-onset disease. The risk of infectionincreases to 15.2% in premature neonates, to 10.7% when the parturient haschorioamnionitis or premature rupture of membranes for more than 24 h and to9.7% if the mother has postpartum bacteremia. In addition to causing perinatal,neonatal and postnatal deaths, neonatal hospital infection is associated with highcosts, as hospitalization is three times longer than in uninfected children. Theidentification of pregnant women colonized by GBS, through universal screening,associated with the adoption of appropriate antibiotics at the time of delivery arethe most successful preventive *** evaluate the sensitivity profile of GBS isolated from pregnant womenattending Vitória da *** This is a cross-sectional study with a quantitative approach carried out in themunicipality of Vitória da Conquista-Bahia between February 2017 and March2018. The study population was composed of 210 pregnant women, with agestational age of 32 to 40 wk, who were aged 18 years or older living in the urbanarea of the municipality of Vitória da Conquista. After a brief explanation aboutthe research and obtaining a signed an informed consent form, data andvaginorectal swabs were collected from the women for GBS research. Examinationof the samples in order to identify the presence of GBS was by culture on sheep blood agar and chromogenic agar for GBS and then, seeded on plates containingstreptococcal culture medium and incubated for 18 h to 24 h at 35°C. Theantimicrobial sensitivity profile of positive GBS samples was determined by thedisk diffusion technique, according to the Clinical and Laboratory StandardsInstitute manual (2017). The data obtained were stored in a database usingMicrosoft Office Excel spreadsheets and a des

关键词： Streptococcus agalactiae Sensitivity profile Pregnancy Clindamycin Erythromycin Group B streptococcal disease

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Prevalence,genotypes and factors associated with HCV infection among prisoners in Northeastern Brazil

引用

World Journal of Gastroenterology 2011年第25期17卷 3027-3034页

作者： Bruno fernandes de Oliveira Santos Nathalie Oliveira de santana Alex Vianey Callado Franca Department of Medicine Federal University of SergipeAracajuSergipe 49060-100Brazil

AIM： To determine hepatitis C virus （HCV） seropreva- lence and its genotypes, and to identify the factors associated with HCV infection. METHODS： This cross-sectional study, conducted in two prisons （one male and one female） in the State of Ser- gipe, Brazil, comprised 422 subjects. All of the prisoners underwent a rapid test for the detection of HCV antibod- ies. Patient~ with a positive result were tested for anti- HCV by enzyme linked immunosorbent assay and for HCV RNA by qualitative polymerase chain reaction （PCR）. The virus genotype was defined in every serum sample that presented positive for PCR-HCV. In order to determine the factors independently associated with positive serol- ogy for HCV, multivariate logistic regression was used. RESULTS： HCV seroprevalence was 3.1%. Of the 13 subjects with positive anti-HCV, 11 had viremia confirmed by PCR. Of these, 90.9% had genotype 1. A total of 43 （10.2%） were injecting drug users, and HCV seroprevalence in this subgroup was 20.6%. The variable most strongly associated with positive serology for HCV was use of injecting drugs [odds ratio （OR）, 23.3; 95% confidence interval （CI）, 6.0-90.8]. Age over 30 years （OR, 5.5; 95%CI, 1.1-29.2）, history of syphilis （OR, 9.8; 95%CI, 1.7-55.2） and history of household contact with HCV positive individual （OR, 14.1; 95%CI, 2.3-85.4） were also independently associated with HCV infection. CONCLUSION： Most of the HCV transmissions result from parenteral exposure. However, there is evidence to suggest a role for sex and household contact with an infected subject in virus transmission.

关键词： Hepatitis C Prisoners Drug abusers Crosssectional analysis Brazil

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Effects of chemical sterilization of the culture media,porous membranes and luminosity on in vitro culture of Eucalyptus grandis×Eucalyptus urophylla

引用

Journal of Forestry Research 2021年第4期32卷 1587-1598页

作者： Letícia Vaz Molinari Denys Matheus santana Costa Souza Maria Lopes Martins Avelar Sérgio Bruno fernandes Douglas Santos Gonçalves Júlio Cézar Tannure Faria Dulcineia de Carvalho Gilvano Ebling Brondani Department of Forestry Sciences Federal University of LavrasLavrasMinas GeraisBrazil

Hybrid combinations of Eucalyptus have increased due to expansion of plantations into unconventional areas and to the search for higher quality ***,most of these species have difficulties surviving in vitro *** chlorine and sealing systems are often used to reduce contamination and increase gas *** aim of the present study is to evaluate the establishment,multiplication,elongation and adventitious rooting of *** × *** clones(C1 and C2) and four active chlorine concentrations(0.000%,0.001%,0.003%,and 0.005%) were tested in the establishment and multiplication *** sealing forms(W/M,1/M and 3/M) and the same four active chlorine concentrations were applied to the elongation *** luminosities(dark and light)and three sealings(W/M,1/M and 3/M) were tested during adventitious *** chlorine concentration of0.005% led to the lowest fungal contamination rate and to the highest in vitro *** chlorine concentration of 0.003% resulted in the greatest length and highest number of shoots per explant in the multiplication *** were no phytotoxicity problems and the quality of plants grown in an environment with active chlorine was maintained in comparison with those grown in an *** increase in gas exchange in ventilation systems had a positive impact on the in vitro growth and development of plants.

关键词： Asepsis Cloning Gas exchange In vitro propagation

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Evolutionary fingerprint, phylogenetic and forest structure of tropical montane Atlantic cloud forests along an elevation gradient

引用

Journal of Mountain Science 2024年第4期21卷 1259-1271页

作者： Ravi fernandes MARIANO Vanessa Leite REZENDE Cléber Rodrigo de SOUZA Patrícia Vieira POMPEU Rubens Manoel dos SANTOS Carolina Njaime MENDES Aloysio Souza de MOURA Felipe santana MACHADO Warley Augusto Caldas CARVALHO Marco Aurélio Leite FONTES Department of Forest Sciences Federal University of LavrasLavras 37200-900Brazil 2Environmental Defence Association of Minas Gerais Belo Horizonte 30360-110Brazil Department of Ecology and Conservation Federal University of LavrasLavras 37200-900Brazil Department of Forest Sciences State University of Mato Grosso do SulAquidauana 79200-000Brazil Agência Zetta Federal University of LavrasLavras 37200-900Brazil. Department of Natural Sciences Federal University of São João del-ReiSão João del-Rei 36307-352Brazil.

Environmental conditions can change markedly over geographical distances along elevation gradients,making them natural laboratories to study the processes that structure *** work aimed to assess the influences of elevation on Tropical Montane Cloud Forest plant communities in the Brazilian Atlantic Forest,a historically neglected *** evaluated the phylogenetic structure,forest structure(tree basal area and tree density)and species richness along an elevation gradient,as well as the evolutionary fingerprints of elevation-success on phylogenetic lineages from the tree *** do so,we assessed nine communities along an elevation gradient from 1210 to 2310 m *** large elevation *** relationships between elevation and phylogenetic structure,forest structure and species richness were investigated through Linear *** occurrence of evolutionary fingerprint on phylogenetic lineages was investigated by quantifying the extent of phylogenetic signal of elevation-success using a genus-level molecular *** results showed decreased species richness at higher elevations and independence between forest structure,phylogenetic structure and *** also verified that there is a phylogenetic signal associated with elevation-success by *** concluded that the elevation is associated with species richness and the occurrence of phylogenetic lineages in the tree communities evaluated in Mantiqueira *** the other hand,elevation is not associated with forest structure or phylogenetic ***,closely related taxa tend to have their higher ecological success in similar ***,we highlight the fragility of the tropical montane cloud forests in the Mantiqueira Range in face of environmental changes(*** warming)due to the occurrence of exclusive phylogenetic lineages evolutionarily adapted to environmental conditions(*** temperature)associated with each elevation range.

关键词： Atlantic Forest Cloud forest Conservation Community ecology Montane forests Phylogenetic diversity Phylogenetic signal

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Correction to:Effects of chemical sterilization of the culture media,porous membranes and luminosity on in vitro culture of Eucalyptus grandis×Eucalyptus urophylla

引用

Journal of Forestry Research 2022年第5期33卷 1705-1707页

Correction to:***://***/10.1007/s11676-020-01240-5 In the original publication of the article,there were errors in the fig 3 and fig *** corrected Figs.3 and 6 are given below:The original article has been corrected.

关键词： corrected porous media

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

FTIR Characterization of Gelatin from Chicken Feet

引用

Journal of Chemistry and Chemical Engineering 2012年第11期6卷 1029-1032页

作者： Poliana fernandes de almeida Suzana Caetano da Silva Lannes Felipe Araujo Calarge Thiago Michel de Brito Farias Jose Carlos Curvelo santana Federal Institute of Education ofMato Grosso Sao Vicente Campus Cuiaba 78106-000 Brazil Faculty of Pharmaceutical Sciences University of Sao Paulo Sao Paulo 05508-000 Brazil Laboratory of Biotechnology and Quality Control Nove de Julho University Sao Paulo 05001-100 Brazil

The objective of this study was characterizing the gelatin from the chicken feet by FTIR （Fourier transform infrared） spectroscopy. Two gelatin samples were prepared for the experiment which were extracted of 200 g of chicken feet by a thermal bath of 4% acetyl acid solution at 60 ℃. A commercial gelatin was used to compare the results. FTIR spectra of gelatin samples were recorded using a Nicolet iS5 FTIR spectrometer equipped with an ATR/iD3 with argon horizontal cell in which the spectra were varied in the range of 400-4,000 cm1 at 16 ~C. The results showed that FTIR spectra of the chicken feet gelatin had the most with the vibration peak at wave numbers of 1,652.01 cm~ to the amide I, of 1,539.87 cm1 to the amide II, of 1,241.29 cm~ to the amide III, of 2,923.72 cm-~ to the amide B and of 3,399.56 cm~ to the amide A. The collagen composition of the chicken feet gelatins was twotimes larger than the commercial gelatin and the vibration bands to beef gelatin have been different from chicken feet gelatins. This demonstrates that chicken feet gelatins are very good nutritional quality when compared to the commercial gelatin.

关键词： FTIR gelatin chicken feet collagen composition.

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Peroxisome proliferator-activated receptors as targets to treat metabolic diseases:Focus on the adipose tissue,liver,and pancreas

引用

World Journal of Gastroenterology 2023年第26期29卷 4136-4155页

作者： Henrique Souza-Tavares Carolline Santos Miranda Isabela Macedo Lopes Vasques-Monteiro Cristian Sandoval Daiana Araujo santana-Oliveira Flavia Maria Silva-Veiga Aline fernandes-da-Silva Vanessa Souza-Mello Department of Anatomy Rio de Janeiro State UniversityRio de Janeiro 20551030Brazil Escuela de Tecnología Médica Facultad de SaludUniversidad Santo TomásOsorno 5310431Chile Departamento de Ciencias Preclínicas Universidad de la FronteraTemuco 4780000Chile

The world is experiencing reflections of the intersection of two pandemics:Obesity and coronavirus disease *** prevalence of obesity has tripled since 1975 worldwide,representing substantial public health costs due to its *** adipose tissue is the initial site of obesity *** excessive energy intake,it undergoes hyperplasia and hypertrophy until overt inflammation and insulin resistance turn adipocytes into dysfunctional cells that send lipotoxic signals to other *** pancreas is one of the organs most affected by *** lipotoxicity becomes chronic,there is an increase in insulin secretion by pancreatic beta cells,a surrogate for type 2 diabetes mellitus(T2DM).These alterations threaten the survival of the pancreatic islets,which tend to become dysfunctional,reaching exhaustion in the long *** for the liver,lipotoxicity favors lipogenesis and impairs beta-oxidation,resulting in hepatic *** silent disease affects around 30%of the worldwide population and can evolve into end-stage liver *** therapy for hepatic steatosis remains to be defined,peroxisome proliferator-activated receptors(PPARs)activation copes with T2DM *** PPARs are transcription factors found at the intersection of several metabolic pathways,leading to insulin resistance relief,improved thermogenesis,and expressive hepatic steatosis mitigation by increasing mitochondrial *** review aimed to update the potential of PPAR agonists as targets to treat metabolic diseases,focusing on adipose tissue plasticity and hepatic and pancreatic remodeling.

关键词： Obesity Insulin resistance Peroxisome proliferator-activated receptors Pancreas Hepatic steatosis Adipose tissue

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Maternal low protein diet induces persistent expression changes in metabolic genes in male rats

引用

World Journal of Diabetes 2020年第5期11卷 182-192页

作者： Allan de Oliveira Lira JoséLuiz de Brito Alves Mariana Pinheiro fernandes Diogo Vasconcelos David Filipe santana João Henrique da Costa-Silva Béatrice Morio Carol Góis Leandro Luciano Pirola Department of Nutrition Federal University of PernambucoVitoria de Santo AntãoPernambuco 55608680Brazil Department of Nutrition Federal University of ParaíbaJoao Pessoa 74556060Brazil Henrique da Costa-Silva Luciano PirolaDepartment of Physical Education and Sport SciencesFederal University of PernambucoVitoria de Santo AntãoPernambuco 55608680Brazil Carmen(Cardiology Metabolism and Nutrition)LaboratoryINSERM U1060Lyon-1 UniversitySouth Lyon Medical FacultyPierre Benite 69310France

BACKGROUND Perinatal exposure to a poor nutritional environment predisposes the progeny to the development of metabolic disease at the adult age,both in experimental models and *** adaptive responses to maternal protein restriction have been reported in metabolic ***,the expression of glucose/fatty acid metabolism-related genes in adipose tissue and liver needs to be *** To evaluate the metabolic impact of perinatal malnutrition,we determined malnutrition-associated gene expression alterations in liver and adipose *** In the present study,we evaluated the alterations in gene expression of glycolytic/Krebs cycle genes(Pyruvate dehydrogenase kinase 4 and citrate synthase),adipogenic and lipolytic genes and leptin in the adipose tissue of offspring rats at 30 d and 90 d of age exposed to maternal isocaloric low protein(LP)diet throughout gestation and *** also evaluated,in the livers of the same animals,the same set of genes as well as the gene expression of the transcription factors peroxisome proliferator-activated receptor gamma coactivator 1,forkhead box protein O1 and hepatocyte nuclear factor 4 and of gluconeogenic *** In the adipose tissue,we observed a transitory(i.e.,at 30 d)downregulation of pyruvate dehydrogenase kinase 4,citrate synthase and carnitine palmitoyl transferase 1b gene *** transcriptional changes did not persist in adult LP rats(90 d),but we observed a tendency towards a decreased gene expression of leptin(P=0.052).The liver featured some gene expression alterations comparable to the adipose tissue,such as pyruvate dehydrogenase kinase 4 downregulation at 30 d and displayed other tissue-specific changes,including citrate synthase and fatty acid synthase upregulation,but pyruvate kinase downregulation at 30 d in the LP group and carnitine palmitoyl transferase 1b downregulation at 90 *** gene alterations,together with previously described changes in gene expression in

关键词： Metabolic adaptation Phenotype plasticity Liver Adipose tissue Metabolism Maternal protein undernutrition Rats

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

<i>In Vitro</i>Effects of Photodynamic Therapy with Three Photosensitizers on <i>Candida</i>spp from Complicated Vulvovaginitis and Asymptomatic Women

引用

Open Journal of Medical Microbiology 2021年第4期11卷 283-297页

作者： Humberto Fabio Boatto Maria Sayonara de Moraes Alexandre Paulo Machado Manoel Joã o Batista Castelo Girã o Gilglécia Novaes Pereira santana Fernanda Aparecida Vieira fernandes Orion Sant’anna Motter Borba Olga Fischman Gompertz Faculty of Medicine University of Mogi das Cruzes Mogi das Cruzes S&#227 o Paulo Brazil Dermatologist of Sao Paulo State Secretary of Health Sao Paulo Brazil Department of Basic Health Sciences Faculty of Medicine Federal University of Mato Grosso Cuiabá Brazil Departament of Ginecology Faculty of Medicine Federal University of S&#227 o Paulo S&#227 o Paulo Brazil Departament of Microbiology Imunology e Parasitology Federal University of S&#227 o Paulo S&#227 o Paulo Brazil

Aim: To evaluate photodynamic therapy (PDT) in vitro to reduce the growth of Candida spp, and its synergy with the antifungals fluconazole and ketoconazole for inhibition of resistant, DDS and susceptible isolates from asymptomatic carriers and with complicated vulvovaginitis. Methods: Between 2017 and 2020, we evaluated 230 women with vulvovaginal candidiasis and 400 asymptomatic. We obtained 130 positive cultures for Candida spp from vulvovaginitis and 94 asymptomatic. Yeasts were characterized by classical and molecular tests. Sensitivity to fluconazole and ketoconazole was evaluated by E-test. We used photodynamic light through blue LED, wavelengths between 450 to 470 nm, power of 260 mW, energy fluence of 270 J/cm2, for 15 minutes over all colonies of Candida spp. Methylene blue (MB) at 450 mg/mL, 2% gentian violet (VG) and 50

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：