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Artemisia vulgaris Induces Tumor-Selective Ferroptosis and Necroptosis via Lysosomal Ca^(2+) Signaling

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Chinese Journal of Integrative Medicine 2024年第6期30卷 525-533页

作者： Lucas dos santos Zamarioli Michele Rosana Maia santos Adolfo Garcia Erustes Vanessa Marques Meccatti Thais Cristine Pereira Soraya S Smaili Maria cristina Marcucci Carlos Rocha Oliveira Gustavo JS Pereira Claudia Bincoletto Department of Pharmacology Paulista School of MedicineFederal University of São PauloSão PauloSP04044-020Brazil Research Group on Phytocomplexes and Cell Signaling School of Health SciencesAnhembi Morumbi UniversitySão PauloSP03164-000Brazil Department of Biosciences and Oral Diagnosis Institute of Science and TechnologySão Paulo State University(ICT-UNESP)São PauloSP12231-280Brazil

Objective To evaluate the chemical composition and effects of Artemisia vulgaris(AV)hydroalcoholic extract(HEAV)on breast cancer cells(MCF-7 and SKBR-3),chronic myeloid leukemia(K562)and NIH/3T3 *** Phytochemical analysis of HEAV was done by high-performance liquid chromatography-mass(HPLC)*** and cell death studies were performed using trypan blue and Annexin/FITC-7AAD,***-1(Fer-1)and necrostatin-1(Nec-1)were used to assess the mode of HEAV-induced cell death and acetoxymethylester(BAPTA-AM)was used to verify the involvement of cytosolic calcium in this *** calcium measurements were made using *** HEAV decreased the viability of MCF-7,SKBR-3 and K562 cells(P<0.05).The viability of HEAV-treated K562 cells was reduced compared to HEAV-exposed fibroblasts(P<0.05).Treatment of K562 cells with HEAV induced cell death primarily by late apoptosis and necrosis in assays using annexin V-FITC/7-AAD(P<0.05).The use of Nec-1 and Fer-1 increased the viability of K562 cells treated with HEAV relative to cells exposed to HEAV alone(P<0.01).HEAV-induced Ca^(2+)release mainly from lysosomes in K562 cells(P<0.01).Furthermore,BAPTA-AM,an intracellular Ca^(2+)chelator,decreased the number of non-viable cells treated with HEAV(P<0.05).Conclusions HEAV is cytotoxic and activates several modalities of cell death,which are partially dependent on lysosomal release of Ca^(2+).These effects may be related to artemisinin and caffeoylquinic acids,the main compounds identified in HEAV.

关键词： Artemisia vulgaris necroptosis ferroptosis late apoptosis Ca^(2+)signaling

Antimicrobial and synergistic effects of lemongrass and geranium essential oils against Streptococcus mutans,Staphylococcus aureus,and Candida spp.

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World Journal of Critical Care Medicine 2024年第3期13卷 63-74页

作者： Patrícia Michelle Nagai de Lima Thaís Cristine Pereira Lara Steffany de Carvalho Letícia Ferreira dos santos Carlos Eduardo Rocha Oliveira Lucas de Paula Ramos Maria cristina Marcucci Amjad Abu Hasna Luciane Dias de Oliveira Department of Biosciences and Oral Diagnosis Institute of Science and TechnologySão Paulo State UniversitySão Josédos Campos 12245000SPBrazil Anhembi Morumbi University School of MedicineSão Josédos Campos 12230-002SPBrazil Department of Restorative Dentistry Endodontics DivisionInstitute of Science and TechnologySão Paulo State UniversitySão Josédos Campos 12245000São PauloBrazil

BACKGROUND The oral cavity harbors more than 700 species of bacteria,which play crucial roles in the development of various oral diseases including caries,endodontic infection,periodontal infection,and diverse oral *** To investigate the antimicrobial action of Cymbopogon Schoenanthus and Pelargonium graveolens essential oils against Streptococcus mutans,Staphylococcus aureus,Candida albicans,***,and *** Minimum microbicidal concentration was determined following Clinical and Laboratory Standards Institute *** synergistic antimicrobial activity was evaluated using the Broth microdilution checkerboard method,and the antibiofilm activity was evaluated with the 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide *** were analyzed by one-way analysis of variance followed by the Tukey post-hoc test(P≤0.05).RESULTS *** and *** essential oils were as effective as 0.12%chlorhexidine against *** and *** monotypic biofilms after 24 *** 24 h *** essential oil at 0.25%was more effective than the nystatin group,and *** essential oil at 0.25%was as effective as the nystatin *** *** and *** essential oils are effective against ***,***,***,***,and *** at different concentrations after 5 min and 24 h.

关键词： Antifungal effect Lemongrass Geranium Candida albicans Candida dubliniensis Candida krusei Staphylococcus aureus Streptococcus mutans

Boll weevils in dry bolls:is there a performance disadvantage?

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Journal of Cotton Research 2024年第3期7卷 243-252页

作者： COELHO Beatriz S. LEITE Suzany A. santos Mateus P.dos MATES Eloito C.de MORAES Beatriz M.L.de BASTOS cristina S. GUEDES Raul N.C. MOREIRA Aldenise A. CASTELLANI Maria A. Departamento de Fitotecnia e Zootecnia Universidade Estadual do Sudoeste da BahiaVitória da ConquistaBA 45031‑900Brazil Faculdade de Agronomia e Veterinária Universidade de BrasíliaBrasíliaDF 70910‑900Brazil Departamento de Entomologia Universidade Federal de ViçosaViçosaMG 36570‑900Brazil Pós‑GraduaCão em Agronomia(Fitotecnia) Universidade Estadual do Sudoeste da BahiaVitória da ConquistaBA 45031‑900Brazil

Background Anthonomus grandis grandis is the main cotton pest in the Americas,with the potential to become a pest in other *** insect uses the plant's reproductive organs for feeding,oviposition,and survival strategies in the off-season,sheltering in dry *** study aimed to know the reproductive potential,feeding,longevity,and morphometric characteristics of boll weevils from dry bolls,squares,and *** were obtained from infested squares,dry bolls,and traps installed in cotton fields and the Caatinga *** weevil pairs were maintained in the laboratory to assess the oviposition,feeding holes,longevity,and morphometric measurements of the parental and F_(1) *** In the parental generation,boll weevils from traps(the Caatinga area and cotton cultivation)showed greater reproductive *** weevils from squares and the Caatinga area showed greater feeding activity compared with those from cotton *** rostrum length,thorax+abdomen length,and abdomen width were observed in boll weevils from cotton cultivation and dry *** origin of boll weevils parental generation did not affect progeny emergence *** F_(1) generation of boll weevils showed significant differences in average longevity depending on the origin of different ***,differences in rostrum lengths were observed,with the boll weevils coming from traps installed in cotton cultivation,the Caatinga area,and dry bolls being the *** thorax+abdomen length and abdomen width,the highest values were observed in those from the Caatinga area and cotton *** It was proven that dry bolls did not affect the reproductive,feeding,and longevity performance of boll weevils,enabling better insect fitness(reproduction and size)in relation to those coming from *** use of dry bolls as off-season shelter is an important survival strategy for the boll weevil.

关键词： Anthonomus grandis grandis Gossypium hirsutum Off-season Survival

Microbial lipases:Propitious biocatalysts for the food industry

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Food Bioscience 2022年第1期45卷 34-44页

作者： Cleonice Aparecida Salgado Clarissa Isabela Aparecida dos santos Maria cristina Dantas Vanetti Department of Microbiology Universidade Federal de Viçosa(UFV)ViçosaMGBrazil

Lipases(triacylglycerol acylhydrolases,EC 3.***.1.3)are one of the largest groups of enzymes and are used in various industrial *** of microbial origin are currently receiving increased attention for industrial application as microorganisms grow quickly and are easily genetically ***,they offer several advantages,such as catalysis of diverse reactions,high specificity,high yields,low energy consumption and reduced processing time and production *** is a relentless ongoing effort to optimise the production of microbial lipases for potential application in the food *** this context,this review highlights the most promising techniques for producing microbial lipases and the recent applications of these lipases in dairy,oils and fats,bakery and confectionery,meat,flavours and aromas and other food *** lipases are normally obtained by fermentation,but the high costs of carbon and nitrogen sources limit the *** overcome this problem,low-cost agro-industrial residues in the lipase production process are *** obtain lipases with high yields and improved characteristics,the technique of protein engineering is described as promising,and the immobilization method that allows the recycling of lipases to improve their catalytic performance is *** to their catalytic properties and versatility,lipases of microbial origin are considered extremely important catalysts in the food industry,meeting the demand for tastier foods with pleasant aromas and ***,microbial lipases are considered safe and sustainable biocatalysts.

关键词： Microbial lipases Food industry Agro-industrial residue

Inherited Fanconi syndrome

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World Journal of Pediatrics 2023年第7期19卷 619-634页

作者： Anna Luiza Braga Albuquerque Rafael dos santos Borges Ana Flavia Conegundes Erika Emmylaine dos santos Frederico Moreira Man Fu Clara Tavares Araujo Pedro Alves Soares Vaz de Castrol Ana cristina Simoes e Silva Interdisciplinary Laboratory of Medical Investigation Unit of Pediatric NephrologyFaculty of MedicineFederal University of Minas Gerais(UFMG)Belo HorizonteBrazil Department of Pediatrics Faculty of MedicineUFMGAlfredo Balena Avenue1902Nd FloorRoom#281Belo HorizonteMG 30130-100Brazil

Background Fanconi-Debré-de Toni syndrome(also known as Fanconi renotubular syndrome,or FRST)profoundly increased the understanding of the functions of the proximal convoluted tubule(PCT)and provided important insights into the pathophysiology of several kidney diseases and drug *** sources We searched Pubmed and Scopus databases to find relevant articles about *** review article focuses on the physiology of the PCT,as well as on the physiopathology of FRST in children,its diagnosis,and *** FRST encompasses a wide variety of inherited and acquired PCT alterations that lead to impairment of PCT *** children,FRST often presents as a secondary feature of systemic disorders that impair energy supply,such as Lowe's syndrome,Dent's disease,cystinosis,hereditary fructose intolerance,galactosemia,tyrosinemia,Alport syndrome,and Wilson's *** rare,congenital causes of FRST greatly impact the morbidity and mortality of patients and impose diagnostic ***,its treatment is diverse and considers the ability of the clinician to identify the correct etiology of the *** The early diagnosis and treatment of pediatric patients with FRST improve the prognosis and the quality of life.

关键词： Cystinosis Fanconi syndrome Fanconi renotubular syndrome Fanconi-Debre-de Toni syndrome Proximal tubule Renal tubular transport-Rickets

Renin-angiotensin system in the pathogenesis of liver fibrosis

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World Journal of Gastroenterology 2009年第21期15卷 2579-2586页

作者： Regina Maria Pereira Robson Augusto Souza dos santos Filipi Leles da Costa Dias Mauro Martins Teixeira Ana cristina Simoes e Silva Department of Pediatrics Faculty of MedicineFederal University of Minas Gerais Laboratory of Hypertension Department of Physiology and BiophysicsInstitute of Biological SciencesFederal University of Minas Gerais Laboratory of Immunopharmacology Department of Biochemistry and ImmunologyInstitute of Biological SciencesFederal University of Minas Gerais

Hepatic fibrosis is considered a common response to many chronic hepatic injuries. It is a multifunctional process that involves several cell types, cytokines, chemokines and growth factors leading to a disruption of homeostatic mechanisms that maintain the liver ecosystem. In spite of many studies regarding the development of fibrosis, the understanding of the pathogenesis remains obscure. The hepatic tissue remodeling process is highly complex, resulting from the balance between collagen degradation and synthesis. Among the many mediators that take part in this process, the components of the Renin angiotensin system （RAS） have progressively assumed an important role. Angiotensin （Ang） II acts as a profibrotic mediator and Ang-（1-7）, the newly recognized RAS component, appears to exert a counter-regulatory role in liver tissue. We briefly review the liver fibrosis process and current aspects of the RAS. This review also aims to discuss some experimental evidence regarding the participation of RAS mediators in the pathogenesis of liver fibrosis, focusing on the putative role of the ACE2-Ang-（1-7）- Mas receptor axis.

关键词： Hepatic fibrosis Renin angiotensin system Angiotensin II Angiotensin-(1-7) Receptor Mas Angiotensin converting enzyme 2

Fluid-dynamics evaluation in a conical spouted bed and characterization of foxtail millet seeds

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Particuology 2015年第6期13卷 75-81页

作者： Lidiane Diniz do.Nascimento Lorena Gomes Corumba Sandra cristina dos santos Rocha Osvaldir Pereira Taranto Cristiane Maria Leal Costa Lenio Jose Guerreiro de Faria Federal University of Pard Faculty of Chemical Engineering Laboratory of Drying and Particle Coating Augusto Correa Street w/n 66075-110 BelemPA Brazil University of Campinas School of Chemical Engineering. Albert Einstein Avenue 500 13083-852 Campinas $8o Paulo Brazil

Foxtail millet （Setaria italica） is one of the most valuable species in economic terms in the genus Setaria and plays an important role in human nutrition, animal feed, and agriculture. The present study described chemical, physical, and quality aspects of seeds of foxtail millet. Furthermore, the fluid-dynamic behavior of the seeds was evaluated in a conical spouted bed, which has advantages in terms of promoting the cyclic and regular movement of the seed particles. Dynamic parameters of spouting （minimum spouting veloc- ity, stable and peak pressure drop） were determined and compared with those obtained from empirical correlations available in the literature. The results obtained from physical characterization showed that the seeds can be classified as belonging to Group D of Geldart, having a non-rough surface, mean diameter of 1.75 mm, and sphericity of 0.74. Fluid-dynamics analysis showed that the seeds are suitable for processing in a spouted bed, which is in agreement with the results of particle physical characterization.

关键词： Foxtail millet Parameters of spouting Characterization Conical spouted bed

Chemical and microbiological characterization of tinctures and microcapsules loaded with Brazilian red propolis extract

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Journal of Pharmaceutical Analysis 2017年第5期7卷 280-287页

作者： Erika Tayse da Cruz Almeida Maria cristina Delgado da Silva José Marcos dos santos Oliveira Regianne Umeko Kamiya Rodolfo Elleson dos santos Arruda Danilo Abreu Vieira Valdemir da Costa Silva Pierre Barnabé Escodro Irinaldo Diniz Basílio-Júnior Ticiano Gomes do Nascimento Laboratório de Controle de Qualidade e Análise de Fármacos Medicamentos e AlimentosCurso de FarmáciaEscola de Enfermagem e FarmáciaUniversidade Federal de AlagoasCampus A.C.SimõesBR 104 Norte-Km 97MaceióAL.CEP:57072-970Brazil Laboratório de Controle de Qualidade Microbiológico de Alimentos Faculdade de NutriçãoUniversidade Federal de AlagoasCampus A.C.SimõesBR 104 Norte/Km 97MaceióAL.CEP:57072-970Brazil

The aim of this study was to characterize tinctures and microcapsules loaded with an ethanol extract of red propolis through chemical, physicochemical and microbiological assays in order to establish quality control tools for nutraceutical preparations of red propolis. The markers(isoflavonoids, chalcones, pterocarpans,flavones, phenolic acids, terpenes and guttiferones) present in the tinctures A and B were identified and confirmed using LC/ESI/FTMS/Orbitrap. Four compositions(A, B, C and D) were prepared to contain B tincture of the red propolis with some pharmaceutical excipients and submitted to two drying processes, i. ***-drying and freeze-drying to obtain microcapsules loaded with the red propolis extract. The tinctures and microcapsules of the red propolis were submitted to the total flavonoid content and antioxidant activity *** antibacterial activity and minimum inhibitory concentration(MIC) were tested using Staphylococcus aureus ATCC 25293 and Pseudomonas aeruginosa ATCC 27853 strains. The tinctures and microcapsules presented high flavonoid quantities from 20.50 to 40.79 mg/100 mg of the microcapsules. The antioxidant activity and IC50 were determined for the tinctures A and B(IC50: 6.95 μg/m L and 7.48 μg/m L), the spray-dried microcapsules(IC50: 8.89–15.63 μg/m L) and the freeze-dried microcapsules(IC50: 11.83–23.36 μg/m L). The tinctures and microcapsules were proved to be bioactive against gram-positive and gram-negative bacteria with inhibition halos superior to 10 mm at concentration of 200 μg/m L and MIC values of 135.87–271.74 μg/m L using gram-positive strain and 271.74–543.48 μg/m L using gram-negative strain. The tinctures and microcapsules of the red propolis have a potential application for nutraceutical products.

关键词： Red propolis Tinctures Microcapsules Isoflavonoids LC/ESI/FTMS/Orbitrap Antioxidant activity Antibacterial activity

Interleukin-1 and TNF-α polymorphisms and Helicobacter pylori in a Brazilian Amazon population

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World Journal of Gastroenterology 2009年第12期15卷 1465-1471页

作者： Hivana Patricia Melo Barbosa Luisa Caricio Martins Sidney Emanuel Batista dos santos Samia Demachki Mnica Baraúna Assumpo Charliana Damasceno Arago Tereza cristina de Oliveira Corvelo Laboratory of Immunogenetics Federal University of Pará66075-110BelémBrazil Nucleus of Tropical Medicine Federal University of Pará66055-240BelémBrazil Laboratory of Human and Medical Genetics Federal University of Pará66075-110BelémBrazil Department of Pathology Joo de Barros Barreto Universitary HospitalFederal University of Pará66073-000BelémBrazil Department of Endoscopy Joo de Barros Barreto Universitary HospitalFederal University of Pará66073-000BelémBrazil

AIM： To study the association between Interleukin-1 （IL-1） and tumor necrosis factor （TNF）-α polymorphisms, infection by Helicobacter pylori （H pylori） and the development of gastrointestinal diseases.METHODS： Genomic DNA was extracted from the peripheral blood of 177 patients with various gastrointestinal diseases and from 100 healthy volunteers. The polymorphisms in IL-1β and TNF-α genes were analyzed using the polymerase chain reactionrestriction fragment length polymorphism method （PCRRFLP） and those from IL-1RN with PCR. The presence of infection due to H pylori and the presence of the CagA toxin were detected by serology. The histopathological parameters in the gastric biopsies of the patients were according to the Sydney classification.RESULTS： A comparison of the frequencies of the different polymorphisms studied among the patients and the control group demonstrated that the allele IL- 1RN＊2 was more frequent among patients with gastric ulcers and adenocarcinoma. Carriers of the allele IL- RN＊2 and those with reactive serology for anti-CagA IgG had a greater risk of developing peptic ulcer and gastric adenocarcinoma, as well as a higher degree of inflammation and neutrophilic activity in the gastricCONCLUSION： Our results indicate a positive association between IL-1RN gene polymorphism and infection by positive H pylori CagA strains and the development of gastric ulcers and adenocarcinoma.

关键词： Helicobacter pylori Interleukin 1β gene Interleukin-1 receptor antagonist gene TNF-α gene Cag pathogenicity island