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Fourier-domain-compressed optical time-stretch quantitative phase imaging flow cytometry

Photonics Research 2024年第8期12卷 1627-1639页

作者： RUBING LI YUEYUN WENG SHUBIN WEI SIYUAN LIN JIN HUANG CONGKUAN SONG HUI SHEN JINXUAN HOU YU XU LIYE MEI DU WANG YUJIE ZOU TAILANG YIN fuling zhou QING GENG SHENG LIU CHENG LEI Institute of Technological Sciences Wuhan University School of Power and Mechanical Engineering Wuhan University Department of Thoracic Surgery Renmin HospitalWuhan University Department of Hematology Zhongnan HospitalWuhan University Department of Thyroid and Breast Surgery Zhongnan HospitalWuhan University Department of Radiation and Medical Oncology Zhongnan HospitalWuhan University School of Computer Science Hubei University of Technology Reproductive Medical Center Renmin HospitalWuhan University Suzhou Institute of Wuhan University Shenzhen Institute of Wuhan University

Optical time-stretch (OTS) imaging flow cytometry offers a promising solution for high-throughput and highprecision cell analysis due to its capabilities of high-speed, high-quality, and continuous imaging. Compressed sensing (CS) makes it practically applicable by significantly reducing the data volume while maintaining its highspeed and high-quality imaging properties. To enrich the information of the images acquired with CS-equipped OTS imaging flow cytometry, in this work we propose and experimentally demonstrate Fourier-domaincompressed OTS quantitative phase imaging flow cytometry. It is capable of acquiring intensity and quantitative phase images of cells simultaneously from the compressed data. To evaluate the performance of our method, static microparticles and a corn root cross section are experimentally measured under various compression ***, to show how our method can be applied in practice, we utilize it in the drug response analysis of breast cancer cells. Experimental results show that our method can acquire high-quality intensity and quantitative phase images of flowing cells at a flowing speed of 1 m/s and a compression ratio of 30%. Combined with machine-learning-based image analysis, it can distinguish drug-treated and drug-untreated cells with an accuracy of over 95%. We believe our method can facilitate cell analysis in both scientific research and clinical settings where both high-throughput and high-content cell analysis is required.

关键词： Image sensors Imaging systems Imaging techniques Phase imaging Refractive index Spatial resolution

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高仿真模拟教学联合护理整合课程教学模式对护理本科生临床实践能力的影响

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中国医学教育技术 2024年第1期38卷 112-117页

作者：裴先波张旭周芙玲武汉大学护理学院武汉430071

目的探讨高仿真模拟教学联合护理整合课程教学模式对护理本科生临床实践能力的影响。方法选取武汉大学护理学院大二、大三及大四的护理本科生为研究对象,分别对应高仿真模拟教学联合护理整合课程教学前、两学期教学后、全部三学期教学... 详细信息

目的探讨高仿真模拟教学联合护理整合课程教学模式对护理本科生临床实践能力的影响。方法选取武汉大学护理学院大二、大三及大四的护理本科生为研究对象,分别对应高仿真模拟教学联合护理整合课程教学前、两学期教学后、全部三学期教学结束后的护理本科生。临床实践能力采用中文版评判性思维倾向量表和中文版Lasater临床判断评分进行评估。结果108人有效完成问卷调查。大二、大三、大四年级评判性思维得分依次逐步提高,其中分析能力(42.16±5.58,44.94±4.60,45.31±4.64,P=0.023)、系统化能力(35.94±7.49,39.82±6.57,42.69±7.02,P=0.001)、自信心(38.59±7.23,41.23±7.16,43.91±5.48,P=0.008)三个维度和总分(276.66±30.70,291.43±32.47,301.78±27.62,P=0.005)差异有统计学意义。三年级[30(27~32)]和四年级学生[31(29~34)]的临床判断总分高于二年级[21(17~23)]学生,差异具有统计学意义(P<0.05);四年级学生高于三年级,但差异无统计学意义(P=0.103)。根据临床判断总分得分分级,在三年级和四年级学生中达到熟练或专家水平逐渐增多(P<0.001)。结论高仿真模拟教学联合护理整合课程教学模式可以有效提高护理本科生临床实践能力,该联合教学模式教学时长是临床实践能力的影响因素。

关键词：护理整合课程高仿真模拟教学临床实践能力

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获得性免疫缺陷综合征合并急性早幼粒细胞白血病1例

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数理医药学杂志 2024年第6期37卷 470-474页

作者：徐丽华吕小艳周芙玲武汉大学中南医院血液科武汉430071

急性早幼粒细胞白血病(acute promyelocytic leukemia,APL)是急性髓系白血病(acute myeloid leukemia,AML)的一种特殊亚型,具有特定的分子发病机制及预后,在获得性免疫缺陷综合征(acquired immunodeficiency syndrome,AIDS)个体中较少见。由于其罕见性和复杂性,AIDS合并APL患者通常需要采用个体化、精准化的治疗策略。本文报道了1例AIDS合并APL的患者,给予拉米夫定、依非韦伦、替诺福韦抗病毒及三氧化二砷(arsenic trioxide,ATO)10 mg·d^(-1)联合口服全反式维甲酸(all-trans retinoic acid,ATRA)60 mg·d^(-1)的诱导治疗后,骨髓提示完全缓解。

关键词：获得性免疫缺陷综合征人类免疫缺陷病毒急性早幼粒细胞白血病急性髓系白血病治疗

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The application of CRISPR-Cas in disease diagnosis and treatment

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中国科学:化学英文版 2023年第10期66卷 2734-2742页

作者： Zhiying Wang Yin Liu fuling zhou Yafen Wang Xiang zhou School of Public Health Wuhan UniversityWuhan 430071China College of Chemistry and Molecular Sciences Wuhan UniversityWuhan 430072China Taikang Center for Life and Medical Sciences Wuhan UniversityWuhan 430071China Cross Research Institute of Zhongnan Hospital Wuhan UniversityWuhan 430071China Department of Hematology of Zhongnan Hospital Wuhan UniversityWuhan 430071China Department of Chemistry College of SciencesHuazhong Agricultural UniversityWuhan 430070China

Clustered regularly interspaced short palindromic repeat(CRISPR)has been gaining much attention in the modern medical field and has been widely used for the diagnosis and treatment of diseases in recent *** this review,we will introduce the application of CRISPR in disease diagnosis and treatment,including its use in detecting pathogens,gene mutations,and genetic diseases,as well as its application in gene therapy for single-gene diseases,cancer,viral infectious diseases,and cardiovascular ***,we will discuss the potential future directions and challenges of CRISPR in the diagnosis and treatment of diseases,and provide a thorough overview of the ways in which CRISPR is used for diagnosing and treating diseases.

关键词： CRISPR application disease diagnosis disease treatment

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Application of super-resolution fluorescence microscopy in hematologic malignancies

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Journal of Innovative Optical Health Sciences 2022年第2期15卷 18-31页

作者： Yalan Yu Jianing Yu Zhen-Li Huang fuling zhou Department of Hematology Zhongnan Hospital of Wuhan UniversityWuhan 430071China Key Laboratory of Biomedical Engineering of Hainan Province School of Biomedical EngineeringHainan UniversityHaikou 570228China

Hematologic malignancies are one of the most common malignant tumors caused by the clonal proliferation and differentiation of hematopoietic and lymphoid stem *** examination of bone marrow cells combined with immunodeficiency typing is of great significance to the diagnostic type,treatment and prognosis of hematologic ***-resolution fluorescence microscopy(SRM)is a special kind of optical microscopy technology,which breaks the resolution limit and was awarded the Nobel Prize in Chemistry in *** the development of SRM,many related technologies have been applied to the diagnosis and treatment of clinical *** was reported that a major type of SRM technique,single molecule localization microscopy(SMLM),is more sensitive than flow cytometry(FC)in detecting cell membrane antigens'expression,thus enabling better chances in detecting antigens on hematopoietic cells than traditional analytic ***,SRM may be applied to clinical pathology and may guide precision medicine and personalized medicine for clone hematopoietic cell *** this paper,we mainly discuss the application of SRM in clone hematological malignancies.

关键词： Hematologic malignancies super-resolution°uorescence microscopy structured illumination microscopy stimulated emission depletion microscopy single molecule localization microscopy

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双打击多发性骨髓瘤的Nomogram预测模型的构建与验证

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临床内科杂志 2024年第8期41卷 535-538页

作者：黎鑫琦商豫凤陈国鹏周芙玲武汉大学中南医院血液内科

目的评估现有分期系统对多发性骨髓瘤(MM)的应用价值，构建双打击MM的Nomogram预测模型并验证。方法回顾性纳入MM患者446例，将其以2∶1的比例随机分为训练组(295例)和验证组(151例)。收集所有患者的基线资料及预后情况并分组进行比... 详细信息

目的评估现有分期系统对多发性骨髓瘤(MM)的应用价值，构建双打击MM的Nomogram预测模型并验证。方法回顾性纳入MM患者446例，将其以2∶1的比例随机分为训练组(295例)和验证组(151例)。收集所有患者的基线资料及预后情况并分组进行比较。采用受试者工作特征(ROC)曲线评估MM患者总生存期(OS)和无进展生存期(PFS)的分层预测价值。采用log-rank检验进行Kaplan-Meier生存曲线差异比较。采用单因素和多因素Cox回归分析评估OS的影响因素。构建Nomogram预后模型，采用C指数评估该模型的预测能力。结果 ROC曲线分析结果显示，R-ISS和D-S对MM患者的OS和PFS分层预测效能不高(P0.05)。多因素Cox回归分析结果显示，年龄、Double-hit、LDH、ISS评分均是影响OS的独立预后因素(P<0.05)。构建包含上述4个独立预后因素的Nomogram预后模型，对其能力进行评价，结果显示在训练组与验证组中预测OS与实际OS之间均具有良好的一致性。结论现有分期系统对MM的生存预测仍然具有一定的局限，建立以Double-hit细胞遗传学异常为基础的Nomogram预后模型具有对OS良好的预测价值，或可用于指导临床个体化治疗。

关键词：双打击多发性骨髓瘤总生存期预后分期系统 Nomogram

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RNA m^(6) A modification: Mapping methods, roles, and mechanisms in acute myeloid leukemia

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Blood Science 2022年第3期4卷 116-124页

作者： Rong Yin Yashu Li Wen Tian fuling zhou Haojian Zhang Department of Hematology Zhongnan HospitalWuhan UniversityWuhanPeople’s Republic of China Frontier Science Center for Immunology and Metabolism Medical Research InstituteWuhan UniversityWuhanPeople’s Republic of China

N^(6)-Methyladenosine(m^(6)A)is the most abundant modification in eukaryotic mRNA,and plays important biological functions via regulating RNA fate *** studies have shown that m^(6)A modification plays a key role in hematologic malignancies,including acute myeloid *** current growth of epitranscriptomic research mainly benefits from technological progress in detecting RNA m^(6)A modification in a transcriptome-wide *** this review,we first briefly summarize the latest advances in RNA m^(6)A biology by focusing on writers,readers,and erasers of m^(6)A modification,and describe the development of high-throughput methods for RNA m^(6)A *** further discuss the important roles of m^(6)A modifiers in acute myeloid leukemia,and highlight the identification of potential inhibitors for AML treatment by targeting of m^(6)A ***,this review provides a comprehensive summary of RNA m^(6)A biology in acute myeloid leukemia.

关键词： Acute myeloid leukemia Mapping RNA m^(6)A modification

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复方苁蓉益智胶囊治疗血管性痴呆的研究进展

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现代养生 2024年第8期24卷 564-567页

作者：邸帅张佳朋刘乙璇李亚楠张江周福玲华北理工大学附属医院神内二、四病区

复方苁蓉益智胶囊具有益智养肝,化浊活血和增智健脑的功效，适用于肝肾亏虚兼痰瘀阻络证的轻中度血管性痴呆。近年来，在血管性痴呆的长期和协同治疗中得到广泛应用，疗效显著。研究总结归纳了复方苁蓉益智胶囊的临床及实验情况，发现... 详细信息

复方苁蓉益智胶囊具有益智养肝,化浊活血和增智健脑的功效，适用于肝肾亏虚兼痰瘀阻络证的轻中度血管性痴呆。近年来，在血管性痴呆的长期和协同治疗中得到广泛应用，疗效显著。研究总结归纳了复方苁蓉益智胶囊的临床及实验情况，发现复方苁蓉益智胶囊可通过降低海马MARKS mRNA的表达、抑制脑组织内氧化应激，保护线粒体，降低脑梗死范围，保护脑缺血损伤和抗血栓形成等机制，治疗血管性痴呆。复方苁蓉益智胶囊与其他抗痴呆常规药物联用或单独使用，可以对血管性痴呆患者的临床症状明显改善，提高生活自理能力和质量。以期为后续复方苁蓉益智胶囊研究提供一定的参考。

关键词：血管性痴呆复方苁蓉益智胶囊痴呆临床应用

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Waking Up Brain with Electrical Stimulation to Boost Memory in Sleep:A Neuroscience Exploration

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Neuroscience Bulletin 2024年第6期40卷 852-854页

作者： Zhe Zhong fuling Yan Chunming Xie Department of Neurology School of MedicineAffiliated Zhongda HospitalSoutheast UniversityNanjing210009China Institute of Neuropsychiatry Affiliated Zhongda HospitalSoutheast UniversityNanjing210009China The Key Laboratory of Developmental Genes and Human Disease Southeast UniversityNanjing210009China

Imagine that,in the future,you could enhance your memory by applying a mild electric current to your brain during *** might sound like science fiction,but it is based on cutting-edge neuroscience *** is widely recognized as a crucial factor for long-term memory consolidation,but the exact mechanisms by which the brain coordinates neural activity across different regions during sleep to reorganize and integrate memories are unknown[1,2].

关键词： Sleep integrate applying

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Balancing electron transfer and intermediate adsorption ability of metallic Ni-Fe-RE-P bifunctional catalysts via 4f-2p-3d electron interaction for enhanced water splitting

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Journal of Energy Chemistry 2024年第7期94卷 458-465页

作者： Hong-Rui Zhao Cheng-Zong Yuan Chenliang zhou Wenkai Zhao Lunliang Zhang Cong-Hui Li Lei Xin fuling Wu Shufeng Ye Xiaomeng Zhang Yunfa Chen School of Rare Earths University of Science and Technology of ChinaHefei 230026AnhuiChina Jiangxi Province Research Institute of Rare Earths Ganjiang Innovation AcademyChinese Academy of SciencesGanzhou 341119JiangxiChina State Key Laboratory of Multiphase Complex Systems Institute of Process EngineeringChinese Academy of SciencesBeijing 100190China

Balancing electron transfer and intermediate adsorption ability of bifunctional catalysts via tailoring electronic structures is crucial for green hydrogen production,while it still remains challenging due to lacking efficient ***,one efficient and universal strategy is developed to greatly regulate electronic structures of the metallic Ni-Fe-P catalysts via in-situ introducing the rare earth(RE)atoms(Ni-Fe-RE-P,RE=La,Ce,Pr,and Nd).Accordingly,the as-prepared optimal Ni-Fe-Ce-P/CC self-supported bifunctional electrodes exhibited superior electrocatalytic activity and excellent stability with the low overpotentials of 247 and 331 mV at 100 mA cm^(-2) for HER and OER,*** the assembled electrolyzer,the Ni-Fe-Ce-P/CC as bifunctional electrodes displayed low operation potential of 1.49 V to achieve a current density of 10 mA cm^(-2),and the catalytic performance can be maintained for 100 *** results combined with density functional theory(DFT)calculation reveal that Ce doping leads to electron decentralization and crystal structure distortion,which can tailor the band structures and d-band center of Ni-Fe-P,further increasing conductivity and optimizing intermediate adsorption *** work not only proposes a valuable strategy to regulate the electron transfer and intermediate adsorption of electrocatalysts via RE atoms doping,but also provides a deep under-standing of regulation mechanism of metallic electrocatalysts for enhanced water splitting.

关键词： RE atoms Electron transfer Adsorption energy Oxygen evolution Hydrogen evolution

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