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温室条件下中棉113对不同地区棉花黄萎病菌的抗性评价

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中国棉花 2024年第1期51卷 11-14页

作者：赵丽红张亚林冯自力魏锋冯鸿杰周京龙朱荷琴中国农业科学院棉花研究所河南安阳455000

为明确中棉113对不同黄萎病菌株的抗性,采用源于新疆、河北和河南的11个不同致病型黄萎病菌株,在温室条件下采用蛭石沙土无底纸钵定量蘸菌液法对中棉113抗病性进行鉴定,分析中棉113对不同致病型菌株的抗性,以及发病情况与生物量指标的... 详细信息

为明确中棉113对不同黄萎病菌株的抗性,采用源于新疆、河北和河南的11个不同致病型黄萎病菌株,在温室条件下采用蛭石沙土无底纸钵定量蘸菌液法对中棉113抗病性进行鉴定,分析中棉113对不同致病型菌株的抗性,以及发病情况与生物量指标的相关性。结果表明:播种后50 d,中棉113的病情指数为19.15~34.82,说明中棉113对这11个菌株均有较好的抗性,达到抗或耐水平。此外,不同致病型黄萎病菌株侵染中棉113对其生物量指标的影响不同,对棉花株高、地上部鲜物质质量和根冠比的影响不显著,而对根长、地下部鲜物质质量存在显著影响。相关分析表明,中棉113病情指数与地下部鲜物质质量、根冠比呈显著正相关关系,与地上部鲜物质质量呈显著负相关关系。将中棉113的黄萎病发生情况与生物量指标相结合可较好地评价其黄萎病抗性,这些结果可为中棉113适宜种植区域的划分提供参考。

关键词：棉花中棉113 黄萎病病害防治抗病性品种区域划分

A review of the pathogenicity mechanism of Verticillium dahliae in cotton

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Journal of Cotton Research 2022年第1期5卷 59-71页

作者： ZHANG Yalin ZHOU Jinglong ZHAO Lihong feng Zili WEI feng BAI Hongyan feng hongjie ZHU Heqin State Key Laboratory of Cotton Biology Institute of Cotton Research of Chi-nese Academy of Agricultural SciencesAnyang 455000HenanChina West-ern Agricultural Research Center of Chinese Academy of Agricultural Sciences Changji 831100XinjiangChina Engineering Research Centre of Cotton Ministry of Education/College of AgricultureXinjiang Agricultural UniversityUrumqi 830052XinjiangChina

Verticillium wilt,caused by the notorious fungal pathogen Verticillium dahliae,is one of the main limiting factors for cotton *** to the stable dormant structure microsclerotia,long-term variability and co-evolution with host plant,its pathogenicity mechanism is very complicated,and the interaction mechanism between pathogen and host plant is also *** identification and functional analysis of the genes involved in the pathogenicity or virulence of this fungus will benefit to uncover the molecular pathogenic mechanism of *** this review,many multifunction genes covering microsclerotia development,pathogen infection,effector proteins,transcription factors,horizontal gene transfer and trans-kingdom RNA silencing have been summarized to provide a theoretical basis to deep understand the molecular pathogenicity mechanism of *** and promote to effectively control Verticillium ***,these pathogenicity-related genes may be considered as targets for effective control of Verticillium wilt in cotton.

关键词： Verticillium dahliae Cotton Pathogenicity-related genes Molecular pathogenic mechanism

Efficacy evaluation and mechanism of Bacillus subtilis EBS03 against cotton Verticillium wilt

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Journal of Cotton Research 2022年第4期5卷 1-11页

作者： BAI Hongyan feng Zili ZHAO Lihong feng hongjie WEI feng ZHOU Jinglong GU Aixing ZHU Heqin PENG Jun ZHANG Yalin State Key Laboratory of Cotton Biology/Institute of Cotton Research of Chinese Academy of Agricultural Sciences Anyang 455000HenanChina Engineering Research Centre of Cotton Ministry of Education/College of AgricultureXinjiang Agricultural UniversityUrumqi 830052XinjiangChina

Background:In our previous study,a strain EBS03 with good biocontrol potential was screened out of 48 strains of cotton endophyte Bacillus subtilis by evaluating the controlling effect against cotton Verticillium ***,its mechanism for controlling Verticillium wilt remains *** objective of this study was to further clarify its con-trolling effect and mechanism against cotton Verticillium ***:The results of confrontation culture test and double buckle culture test showed that the inhibitory effects of EBS03 volatile and nonvolatile metabolite on mycelium growth of Verticillium dahliae were 70.03%and 59.00%,respectively;the inhibitory effects of sporulation and microsclerotia germination were 47.16%and 70.06%,*** the greenhouse test,the EBS03 fermentation broth root irrigation had the highest controlling effect at 87.11%on cotton Verticillium wilt,and significantly promoted the growth of cotton *** the field experi-ment,the controlling effect of EBS03 fermentation broth to cotton Verticillium wilt was 42.54%at 60 days after cotton sowing,and the boll number per plant and boll weight in EBS03 fermentation broth seed soaking,root irrigation,and spraying treatments significantly increased by 19.48%and 7.42%,30.90%and 2.62%,15.99%and 9.20%,***,EBS03 improved the resistance of cotton leaves against the infection of ***,and induced the outbreak of reactive oxygen species and accumulation of *** addition,the results of real time fluorescent quantitative polymerase chain reaction(RT-qPCR)detection showed that EBS03 significantly induced upregulation expression level of defense-related genes PAL,POD,PPO,and PR10 in cotton leaves,enhanced cotton plant resistance to ***,and inhibited colonization level of this fungal pathogen in ***:Bacillus subtilis EBS03 has a good biological defense capability,which can inhibit the growth and coloni-zation level of ***,and activate the

关键词： Endophytic bacteria Bacillus subtilis Cotton Verticillium wilt Control mechanism Induced resistance

防治棉花黄萎病的枯草芽孢杆菌EBS03发酵条件优化

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中国棉花 2024年第4期51卷 35-40页

作者：郭世杰白红燕张东风杨金霞冯自力冯鸿杰魏锋周京龙赵丽红朱荷琴毛鹏志张亚林中国农业科学院棉花研究所/棉花生物育种与综合利用全国重点实验室河南安阳455000 奎屯华棉农业科技有限公司新疆奎屯833200 新疆生产建设兵团第七师农业科学研究所新疆奎屯833200 新疆昌吉市农业技术推广中心新疆昌吉831100

本团队开展了48株枯草芽孢杆菌对棉花黄萎病防治效果评测,筛选出对棉花黄萎病防治效果良好、具有促进棉花生长作用的Bacillus subtilis EBS03。本研究以EBS03菌株为研究对象,利用单因素试验及正交试验对培养基成分及发酵条件进行优化,... 详细信息

本团队开展了48株枯草芽孢杆菌对棉花黄萎病防治效果评测,筛选出对棉花黄萎病防治效果良好、具有促进棉花生长作用的Bacillus subtilis EBS03。本研究以EBS03菌株为研究对象,利用单因素试验及正交试验对培养基成分及发酵条件进行优化,充分发挥EBS03菌株生长潜力。通过对不同种类的碳源、氮源及无机盐的筛选,确定最优发酵培养基配方;进一步对EBS03菌株的培养温度、装液量、初始接种量、转速和初始pH进行优化,以确定最优培养条件。结果表明,*** EBS03菌株最佳培养条件为培养温度37℃、装液量50 mL、初始接菌量4%(体积分数)、转速200 r·min^(-1)、初始pH 7;优化后的培养基成分为甘露醇1.8 g、蛋白胨2.1 g和硫酸镁1.9 g,在此最优条件下,EBS03菌株发酵液的最高菌体含量为有效活菌数9.60×10^(7)mL^(-1)。优化后的培养基配方和发酵条件可为EBS03菌株的高效发酵奠定基础。

关键词：棉花黄萎病枯草芽孢杆菌生物防治培养基成分发酵条件优化

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Utilizing dual-pathway energy transfer in upconversion nanoconjugates for reinforced photodynamic therapy

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Nano Research 2024年第4期17卷 2941-2948页

作者： Ruohao Zhang Yu Lu Yifei Zhou Kehong Lv Xinyu Fu Jitong Gong Shuang Yao Xiaozhen Wang Jing feng hongjie Zhang State Key Laboratory of Rare Earth Resource Utilization Changchun Institute of Applied ChemistryChinese Academy of SciencesChangchun 130022China School of Applied Chemistry and Engineering University of Science and Technology of ChinaHefei 230026China College of Chemistry Jilin UniversityChangchun 130012China Department of Breast Surgery General Surgery Centerthe First Hospital of Jilin UniversityChangchun 130021China Department of Chemistry Tsinghua UniversityBeijing 100084China

Enhancing the therapeutic effect of existing treatments or developing new non-invasive treatments are important measures to achieve high-efficiency treatment of malignant *** therapy(PDT)is an emerging treatment modality,and the key for achieving high-efficiency PDT is to select light with strong tissue penetration depth and enhance the generation of reactive oxygen species(ROS).Although the upconversion nanoparticles(UCNPs)modified with the photosensitizers could achieve PDT with strong penetration depth under near-infrared light irradiation,the ROS generated by traditional single-pathway PDT is still ***,we developed a novel nanoconjugate(UCNP-Ce6/AIEgen)for dual-pathway reinforced PDT,in which the UCNPs were co-modified with chlorin e6(Ce6)and luminogen with aggregation-induced emission(AIEgen).Due to the presence of AIEgen,UCNP-Ce6/AIEgen could avoid aggregation-caused luminescence quenching in biological water environments and convert upconversion luminescence(UCL)of UCNPs to Ce6-activatable ***,under the irradiation of 808 nm laser,UCNP-Ce6/AIEgen can not only undergo direct lanthanide-triplet energy transfer to activate Ce6,but also convert the UCL of UCNPs to the light that can activate Ce6 through Fӧrster resonance energy transfer to generate more ROS,thus promoting tumor cell *** work broadens the applications of nanoconjugates of lanthanide-based inorganic materials and organic dyes,and provides a conception for reinforced PDT of tumors.

关键词： upconversion nanoparticle aggregation-induced emission direct lanthanide-triplet energy transfer Förster resonance energy transfer photodynamic therapy

Calcium-chelating peptides from rabbit bone collagen:characterization,identification and mechanism elucidation

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Food Science and Human Wellness 2024年第3期13卷 1485-1493页

作者： Fuhuan Yuan Yu Fu Liang Ma Hankun Zhu Yong Yu Xin feng Yi Sun hongjie Dai Xin Liu Zhengfang Liu Yuhao Zhang College of Food Science Southwest UniversityChongqing 400715China Chongqing Key Laboratory of Speciality Food Co-Built by Sichuan and Chongqing Chongqing 400715China Key Laboratory of Luminescence Analysis and Molecular Sensing(Southwest University) Ministry of EducationChongqing 400715China Angel Yeast Co. Ltd.Yichang 443003China

This study aimed to characterize and identify calcium-chelating peptides from rabbit bone collagen and explore the underlying chelating *** peptides and calcium were extracted from rabbit bone by instant ejection steam explosion(ICSE)combined with enzymatic hydrolysis,followed by chelation reaction to prepare rabbit bone peptide-calcium chelate(RBCP-Ca).The chelating sites were further analyzed by liquid chromatography-tandem mass(LC-MS/MS)spectrometry while the chelating mechanism and binding modes were *** structural characterization revealed that RBCP successfully chelated with calcium ***,LC-MS/MS analysis indicated that the binding sites included both acidic amino acids(Asp and Glu)and basic amino acids(Lys and Arg),Interestingly,three binding modes,namely Inter-Linking,Loop-Linking and Mono-Linking were for the first time found,while Inter-Linking mode accounted for the highest proportion(75.1%),suggesting that chelation of calcium ions frequently occurred between two ***,this study provides a theoretical basis for the elucidation of chelation mechanism of calcium-chelating peptides.

关键词： Rabbit bone Collagen peptide Peptide-calcium chelate Chelation mechanism Liquid chromatography-tandem mass(LC-MS/MS)

Succinate dehydrogenase SDH1–1 positively regulates cotton resistance to Verticillium dahliae through a salicylic acid pathway

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Journal of Cotton Research 2020年第2期3卷 103-114页

作者： ZHANG Xiangyue feng Zili ZHAO Lihong LIU Shichao WEI feng SHI Yongqiang feng hongjie ZHU Heqin State Key Laboratory of Cotton Biology Institute of Cotton Research of Chinese Academy of Agricultural SciencesAnyang 455000HenanChina Zhengzhou Research Base State Key Laboratory of Cotton BiologySchool of Agricultural SciencesZhengzhou UniversityZhengzhou 450001China.

Background:Verticillium wilt,caused by the soil-borne fungus of Verticillium dahliae Kleb.,is one of the most devastating diseases of *** complex mechanism underlying cotton resistance to Verticillium wilt remains *** an endogenous resistance gene may be helpful to control this *** studies revealed that succinate dehydrogenase(SDH)is involved in reactive oxygen species(ROS)-induced stress signaling pathway that is likely to be triggered by salicylic acid(SA).Here,through the metabolomics and differential expression analyses in wilt-inoculated cotton(Gossypium hirsutum),we noticed that Gh SDH1–1 gene in cotton may play an important role in the resistance to *** we reported Gh SDH1–1 gene and its functional analysis in relation to the resistance of cotton to ***:The Gh SDH1–1 gene in cotton root was significantly up-regulated after *** inoculation,and its expression level peaked at 12 and 24 h *** can also induce the up-regulation of Gh SDH1–***,the functional analysis showed that Gh SDH1–1-silenced cotton was more susceptible to *** than the control because of the significant decrease in abundance of immune-related molecules and severe damage to the SA-signaling *** Arabidopsis thaliana,high expression of Gh SDH1–1 conferred high resistance to *** that overexpressed Gh SDH1–1 had higher resistance to *** infection compared with the ***:Our findings provide new insights into the role of Gh SDH1–1;it positively regulates cotton resistance to Verticillium *** regulatory mechanism of Gh SDH1–1 is closely related to SA-related signaling pathway.

关键词： Cotton Verticillium wilt Resistance gene GhSDH1–1 Salicylic acid

Fine Tuning of the Electronic Properties of Novel BTPE Using Oligosilanyl Linkages and Their Application in Rapid High-Resolution Visualization of Latent Fingerprints

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CCS Chemistry 2020年第3期2卷 329-336页

作者： Xiaoshuang Xiang Zhikuan Zhou hongjie feng Siyang feng Lizhi Gai Hua Lu Zijian Guo Key Laboratory of Organosilicon Chemistry and Material Technology of Ministry of Education and Key Laboratory of Organosilicon Material of Zhejiang ProvinceHangzhou Normal UniversityHangzhou 311121 State Key Laboratory of Coordination Chemistry School of Chemistry and Chemical EngineeringNanjing UniversityNanjing 210023.

Structural modifications throughπ-interactions usually result in redshifts in luminescence and,as a consequence,the loss of the natural color of the ***,employing Si-Siσ-bridging to manipulate the electronic properties of organic materials has remained largely ***,we report a series of novel bis-tetraphenylethenes(BTPEs)with oligosilanyl linkages,termed BTPE-Sin molecules,used to manipulate the photophysical properties of luminogens subtly throughσ–π*** oligosilanyl-bridged molecules were thermally,highly stable,and exhibited enhanced aggregation-induced emissions,as well as luminescence efficiencies while retaining most of their original *** current BTPEs fabrications have easy-to-operate,fast,and high-resolution identification properties toward ***,they are highly specific to individuals,and hence,vital in forensic *** achieved these features through the introduction of oligosilanyl chains that increased the lipophilicity of the *** work offers a universal and straightforward approach for the generation of highly emissive organic materials and enables fine-tuning of their electronic properties for multifunctional applications.

关键词： AIE σ–πconjugation dyes/pigments LFP visualization luminescence

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有轨制导车堆垛的动态稳定性研究

计算机集成制造系统 2024年第1期30卷 316-328页

作者：尹昊董益民冯卓和红杰陈帆西南交通大学信息科学与技术学院四川成都611756 西南交通大学计算机与人工智能学院四川成都611756

有轨制导车(RGV)堆垛是智能仓储的关键一环,运动时倒塌不仅会降低工作效率,还可能带来货物损坏、轨道变形等安全隐患。为提高智能仓储系统的工作效率,从箱子位置和刚体受力的角度,提出一种RGV堆垛的可承载加速度(即不使任何箱子倒塌的... 详细信息

有轨制导车(RGV)堆垛是智能仓储的关键一环,运动时倒塌不仅会降低工作效率,还可能带来货物损坏、轨道变形等安全隐患。为提高智能仓储系统的工作效率,从箱子位置和刚体受力的角度,提出一种RGV堆垛的可承载加速度(即不使任何箱子倒塌的最大加速度值)计算方法。首先,根据加速度方向和箱子的位置,将堆垛中箱子划分为多个刚体,每个刚体包含会一起倒塌的多个箱子;然后,根据堆垛中刚体的位置构建刚体树,分析刚体之间的通过树传递的阻倒力;最后根据阻倒力计算RGV堆垛的可承载加速度。提出的可承载加速度不仅可用于限制RGV运动加速度,还可用于定量评估堆垛的动态稳定性。实验结果表明,可承载加速度计算结果与Unity仿真堆垛的动态稳定性之间,呈现皮尔逊相关系数>0.8的极强线性相关性,优于现有的堆垛动态稳定性评估方法。

关键词：智能仓储有轨制导车堆垛动态稳定性可承载加速度阻倒力