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Biosynthesis of artemisinic acid in engineered Saccharomyces cerevisiae and its attractiveness to the mirid bug Apolygus lucorum

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Journal of Integrative Agriculture 2022年第10期21卷 2984-2994页

作者： TENG Dong LIU dan-feng Khashaveh ADEL SUN Pei-yao GENG Ting ZHANG Da-wei ZHANG Yong-jun State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant ProtectionChinese Academy of Agricultural SciencesBeijing 100193P.R.China Laboratory of Ecology and Evolutionary Biology Yunnan UniversityKunming 650091P.R.China Langfang Scientific Research Trial Station Institute of Plant ProtectionChinese Academy of Agricultural SciencesLangfang 065000P.R.China Institute of Plant Protection Gansu Academy of Agricultural SciencesLanzhou 730070P.R.China

Artemisia annua is an important preferred host of the mirid bug Apolygus lucorum in *** emitted from *** attract *** artemisinic acid of *** is a precursor of artemisinin that has been widely investigated in the Chinese herbal medicine ***,little is known at this point about the biological roles of artemisinic acid in regulating the behavioral trends of *** this study,we collected volatiles from *** at the seedling stage by using headspace solid phase microextraction(HS-SPME).Gas chromatography-mass spectrometry(GC-MS) analysis showed that approximately 11.03±6.00 and 238.25±121.67 ng hartemisinic acid were detected in volatile samples and milled samples,***,a key gene for artemisinic acid synthesis,the cytochrome P450 gene cyp71 av1,was expressed in engineered Saccharomyces cerevisiae to catalyze the production of artemisinic *** the addition of exogenous artemisinic alcohol or artemisinic aldehyde,artemisinic acid was identified as the product of the expressed *** electroantennogram(EAG) recordings,3-day-old adult *** showed significant electrophysiological responses to artemisinic alcohol,artemisinic aldehyde and artemisinic ***,3-day-old female bugs were significantly attracted by artemisinic acid and artemisinic alcohol at a concentration of 10 mmol L,whereas 3-day-old male bugs were attracted significantly by 10 mmol Lartemisinic acid and artemisinic *** propose that artemisinic acid and its precursors could be used as potential attractant components for the design of novel integrated pest management strategies to control ***.

关键词： artemisinic acid CYP71AV1 biosynthesis Apolygus lucorum electrophysiological responses behavioral trends

Long-term survival of a patient with a large adrenal primitive neuroectodermal tumor: A case report

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World Journal of Clinical Cases 2019年第3期7卷 340-346页

作者： Jun Dai Hong-Chao He Xin Huang Fu-Kang Sun Yu Zhu dan-feng Xu Department of Urology Ruijin Hospital Shanghai Jiaotong University School of Medicine

BACKGROUND Adrenal primitive neuroectodermal tumor(PNET) is an extremely rare malignant tumor with poor prognosis and of neural crest origin. Herein, we report a case of adrenal PNET and summarized its clinical and pathological characteristics on the basis of 16 patients reported *** SUMMARY A female patient aged 25 years presented with right lumbago for 12 mo, and preoperative computed tomography showed a huge right adrenal mass. She received tumorectomy, and post-operative pathological examination showed adrenal PNET. After surgery, she underwent adjuvant chemotherapy and was followed up 31 mo after surgery. She received brachytherapy for right paracolic and hepatic metastases. She was alive and followed up for 60 mo. In available studies, only 57.14%(4/7) and 44.44%(4/9) were positive for the expression of neuron-specific enolase and synaptophysin, respectively, although CD99 expression was found in all the patients(100%; 10/10).CONCLUSION It is concluded that adrenal PNET is very rare and highly malignant, and histology is a golden standard in its diagnosis. Surgery and adjuvant therapy is the main treatment.

关键词： Primitive neuroectodermal tumor Adrenal gland Case report

Rare empty sella syndrome found after postoperative hypotension and respiratory failure:A case report

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World Journal of Clinical Cases 2019年第5期7卷 663-667页

作者： Peng Guo Zeng-Jun Xu Chang-En Hu Yue-Ying Zheng dan-feng Xu Department of Anesthesiology Shaoxing Central Hospital Department of Anesthesiology the First Affiliated Hospital of Zhejiang University

BACKGROUND Empty sella syndrome is a condition in which the pituitary gland shrinks or flattens. Patients with empty sella syndrome often present with headache,hypertension, obesity, visual disturbances, cerebrospinal fluid(CSF) rhinorrhoea,or endocrine dysfunction. Herein, we report a rare case of empty sella syndrome discovered after the patient experienced postoperative hypotension and respiratory *** SUMMARY A 60-year-old man was admitted for further workup of left shoulder pain. He was assessed by the orthopaedics team and booked for internal fixation of the left clavicle. General anaesthesia with a nerve block was administered. His blood pressure continued to decrease post-operation. Endocrine tests were performed,with the results supporting a diagnosis of hypopituitarism with hypocortisolism and hypothyroidism. Brain magnetic resonance imaging demonstrated that the sella was enlarged and filled with CSF, confirming a diagnosis of empty sella syndrome. The patient was started on endocrine replacement therapy. The patient regained consciousness and spontaneous breath *** This case highlights the importance of considering pituitary hormone insufficiency in the context of respiratory and hemodynamic failure during the perioperative period.

关键词： Empty sella Hypotension Respiratory failure Case report

Enhancing security of NVM-based main memory with dynamic Feistel network mapping

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Frontiers of Information Technology & Electronic Engineering 2018年第7期19卷 847-863页

作者： Fang-ting HUANG dan feng Wen XIA Wen ZHOU Yu-cheng ZHANG Min FU Chun-tao JIANG Yu-kun ZHOU Wuhan National Laboratory for Optoelectronics Wuhan 430074 China MOE Key Laboratory of Information Storage System Wuhan 430074 China School of Computer Science and Technology Huazhong University of Science and Technology Wuhan 430074 China

As promising alternatives in building future main memory systems, emerging non-volatile memory(NVM) technologies can increase memory capacity in a cost-effective and power-efficient way. However, NVM is facing security threats due to its limited write endurance: a malicious adversary can wear out the cells and cause the NVM system to fail quickly. To address this issue, several wear-leveling schemes have been proposed to evenly distribute write traffic in a security-aware manner. In this study, we present a new type of timing attack, remapping timing attack(RTA), based on information leakage from the remapping latency difference in NVM. Our analysis and experimental results show that RTA can cause three of the latest wear-leveling schemes(i.e., region-based start-gap,security refresh, and multi-way wear leveling) to lose their effectiveness in several days(even minutes), causing failure of NVM. To defend against such an attack, we further propose a novel wear-leveling scheme called the ‘security region-based start-gap(security RBSG)', which is a two-stage strategy using a dynamic Feistel network to enhance the simple start-gap wear leveling with level-adjustable security assurance. The theoretical analysis and evaluation results show that the proposed security RBSG not only performs well when facing traditional malicious attacks, but also better defends against RTA.

关键词： Non-volatile memory (NVM) Endurance Wear leveling Timing attack

Spectrophotometric Determination of Arginine in Grape Juice Using 8-Hydroquinoline

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Agricultural Sciences in China 2008年第10期7卷 1210-1215页

作者： WANG Hua LIANG Xin-hong ZHAO Rui-xiang feng Li-dan LI Hua College of Enology Northwest A ＆ F University Yangling 712100 P.R.China School of Food Science Henan Institute of Science and Technology Xinxiang 453003 P.R.China Shaanxi Engineering Research Center for Viti-Viniculture Yangling 712100 P.R.China

Arginine in grape juice can be metabolized by wine yeasts and malolactic bacteria to precursors of ethyl carbamate, known as carcinogen. The aim of this study was to develop a simple, fast, and accurate method for determining arginine in grape juice with Sakaguchi reaction by separating arginine with strong cation-exchange resins. Parameters were optimized including the concentrations of 8-hydroquinoline and sodium hydrobromite. The color stability lasted for 4 min, which is sufficient to finish the measurement. The method is simple, reproducible and accurate, and can be applied for quick measurement of arginine in grape juice to take necessary measures for controlling the level of ethyl carbamate.

关键词： grape juice L-arginine ethyl carbamate Sakaguchi reaction

Facile Synthesis of Porous Carbon Nitride Spheres with Hierarchical Three-Dimensional Mesostructures for CO_(2) Capture

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Nano Research 2010年第9期3卷 632-642页

作者： Qiang Li Jianping Yang dan feng Zhangxiong Wu Qingling Wu Sung Soo Park Chang-Sik Ha Dongyuan Zhao Department of Chemistry and Shanghai Key Laboratory of MolecularCatalysis and Innovative Materials Laboratory of Advanced MaterialsFudan UniversityShanghai 200433China Department of Polymer Science and Engineering Pusan National UniversityGeumjeong-guBusan 609-735Korea

Porous carbon nitride(CN)spheres with partially crystalline frameworks have been successfully synthesized via a nanocasting approach by using spherical mesoporous cellular silica foams(MCFs)as a hard template,and ethylenediamine and carbon tetrachloride as *** resulting spherical CN materials have uniform diameters of ca.4μm,hierarchical three-dimensional(3-D)mesostructures with small and large mesopores with pore diameters centered at ca.4.0 and 43 nm,respectively,a relatively high BET surface area of~550 m^(2)/g,and a pore volume of 0.90 cm^(3)/***-resolution transmission electron microscope(HRTEM)images,wide-angle X-ray diffraction(XRD)patterns,and Raman spectra demonstrate that the porous CN material has a partly graphitized *** addition,elemental analyses,X-ray photoelectron spectra(XPS),Fourier transform infrared spectra(FT-IR),and CO_(2) temperature-programmed desorption(CO_(2)-TPD)show that the material has a high nitrogen content(17.8 wt%)with nitrogen-containing groups and abundant basic *** hierarchical porous CN spheres have excellent CO_(2) capture properties with a capacity of 2.90 mmol/g at 25℃and 0.97 mmol/g at 75℃,superior to those of the pure carbon materials with analogous *** can be mainly attributed to the abundant nitrogen-containing basic groups,hierarchical mesostructure,relatively high BET surface area and stable ***,the presence of a large number of micropores and small mesopores also enhance the CO_(2) capture performance,owing to the capillary condensation effect.

关键词： Mesoporous materials carbon nitride nanocasting sphere hard template CO_(2)capture

Comparative Analysis of Gene Expression in Two Muskmelon Cultivars(Cucumis melo L.) Under Salt Stress

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Journal of Integrative Agriculture 2014年第10期13卷 2132-2140页

作者： WEI Shi-wei ZHANG Fu-rong ZHANG Yi-dong WANG Li-min CHEN Jia-bei HUANG dan-feng Department of Plant Science School of Agriculture and BiologyShanghai Jiao Tong University/Key Laboratory of Urban Agriculture (South)Ministry of Agriculture

Salinity is one of the most important abiotic stresses that adversely affects crop growth and productivity. A subtractive suppression hybridization （SSH） library were constructed from the roots of salt-sensitive Yulu cultivar melon seedlings under salt stress; 557 high-quality expressed sequence tags （ESTs） were randomly sequenced, with an average size of 428 bp, which assembled into 68 contigs and 315 singletons. Compared with our previous SSH library generated from the salt-tolerant Bingxuecui cultivar, the proportion of transcripts involved in metabolism, protein fate, cellular communication/signal transduction mechanisms, and cell rescue/defense were 4, 1.46, 0.94, and 0.4% higher, respectively, in the salt-tolerant cultivar than the in salt-sensitive cultivar. Quantitative real-time PCR analysis of eleven transcripts revealed temporal variations in their expression in the two cultivars under salt stress. One NAC gene （JZ477011） was heterologously expressed in yeast for functional characterization, and enhanced the sensitivity of yeast cells to high-salinity to salt stress and inhibited their growth. Information regards to their functions would aid in the understanding of response mechanisms to saline stress and in the development of molecular markers for selecting salt-tolerant melon cultivars.

关键词： comparative analysis functional characterization gene expression salt stress SSH melon cultivars

A novel Li-ion supercapattery by K-ion vacant ternary perovskite fluoride anode with pseudocapacitive conversion/insertion dual mechanisms

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Rare Metals 2022年第7期41卷 2491-2504页

作者： Yong-Fa Huang Rui Ding dan-feng Ying Yu-Xi Huang Tong Yan Cai-Ni Tan Xiu-Juan Sun En-Hui Liu Key Laboratory of Environmentally Friendly Chemistry and Applications of Ministry of Education College of ChemistryXiangtan UniversityXiangtan 411105China

An innovative K+vacant ternary perovskite fluoride(K_(0.89)Ni_(0.02)Co_(0.03)Mn_(0.95)F_(3.0),KNCMF-3#)anode was designed for advanced Li-ion supercapattery(i.e.,Li-ion capacitors/batteries,LIC/Bs).Owing to the conversion/insertion dual mechanisms and fast pseudocapacitive con-trol dynamics,the KNCMF-3#electrode exhibits superior electrochemical performance,especially the excellent cycle performance(467%(229 mAh·g^(-1))/1000 cycles/2 A·g^(-1)).Moreover,the hybrid KNCMF-3#/reduced gra-phene oxide(rGO)electrode can further increase the electrochemical performance(217-97 mAh·g^(-1)/0.1-3.2 A·g^(-1),150%(197 mAh·g^(-1))/1000 cycles/2 A·g^(-1)).Also,a novel capacitor/battery cathode,activated carbon(AC)+LiFePO_(4)+graphene(AC+LFP+G),exhibits impres-sive performance(128-82 mAh·g^(-1)/0.1-3.2 A·g^(-1),84%/1000 cycles/2 A·g^(-1)).By the synergistic optimization of anode and cathode,the Li-ion supercapattery KNCMF-3#@rGO//AC+LFP+G demonstrates remarkable per-formance,for example,111.9-23.8 Wh·kg^(-1)/0.4-8.0 kW·kg^(-1)/82%/2000 cycles/5 A·g^(-1)/0-4 V,which is superior to KNCMF-3#//AC LICs,KNCMF-3#@rGO//AC LICs,KNCMF-3#//AC+LFP+G LIC/*** all,the novel Li-ion supercapattery idea adds a promising per-spective to develop advanced energy storage devices.

关键词： Perovskite fluoride Defect Anode Li-ion supercapattery Pseudocapacitive

Biofilm Disruption Utilizing α/β Chimeric Polypeptide Molecular Brushes

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Chinese Journal of Polymer Science 2019年第11期37卷 1105-1112页

作者： Si Zhang Xi-Mian Xiao Fan Qi Peng-Cheng Ma Wei-Wei Zhang Cheng-Zhi Dai dan-feng Zhang Run-Hui Liu State Key Laboratory of Bioreactor Engineering Key Laboratory for Ultrafine Materials of Ministry of Education Research Center for Biomedical Materials of Ministry of Education School of Materials Science and Engineering East China University of Science and Technology

Gram-negative bacteria can cause serious infections and are well known problems in biomedical practices. Biofilms of gramnegative bacteria are notorious for their frequently encountered resistance toward antibiotics. We demonstrate that α/β chimeric polypeptide molecular brush (α/β CPMB) exerts potent activities against antibiotic-resistant gram-negative bacteria. MTT viability assay, bacterial colony counting, and live/dead staining all indicate that α/β CPMB not only inhibits biofilm formation of gram-negative Pseudomonas aeruginosa and Acinetobacter baumannii, but also effectively disrupts mature biofllms that are highly resistant to one of the most active antibiotics-colistin. The superior antibacterial performance of the α/β CPMB implies its potential topical applications in treating biofilms.

关键词： Molecular brush Chimeric polypeptide Biofilm Antimicrobial resistance Host defense peptide