Biosynthesis of artemisinic acid in engineered Saccharomyces cerevisiae and its attractiveness to the mirid bug Apolygus lucorum

作     者：TENG Dong LIU Dan-feng Khashaveh ADEL SUN Pei-yao GENG Ting ZHANG Da-wei ZHANG Yong-jun TENG Dong;LIU Dan-feng;Khashaveh ADEL;SUN Pei-yao;GENG Ting;ZHANG Da-wei;ZHANG Yong-jun

作者机构：State Key Laboratory for Biology of Plant Diseases and Insect PestsInstitute of Plant ProtectionChinese Academy of Agricultural SciencesBeijing 100193P.R.China Laboratory of Ecology and Evolutionary BiologyYunnan UniversityKunming 650091P.R.China Langfang Scientific Research Trial StationInstitute of Plant ProtectionChinese Academy of Agricultural SciencesLangfang 065000P.R.China Institute of Plant ProtectionGansu Academy of Agricultural SciencesLanzhou 730070P.R.China 

出 版 物：《Journal of Integrative Agriculture》 (农业科学学报（英文版）)

年 卷 期：2022年第21卷第10期

页      面：2984-2994页

核心收录：

学科分类：090403[农学-农药学(可授农学、理学学位）] 09[农学] 0904[农学-植物保护] 

基　　金：supported by the National Natural Science Foundation of China (31772176 and 31972338) the National Key Research and Development Program of China (2019YFD0300100) 

主　　题：artemisinic acid CYP71AV1 biosynthesis Apolygus lucorum electrophysiological responses behavioral trends 

摘      要：Artemisia annua is an important preferred host of the mirid bug Apolygus lucorum in *** emitted from *** attract *** artemisinic acid of *** is a precursor of artemisinin that has been widely investigated in the Chinese herbal medicine ***,little is known at this point about the biological roles of artemisinic acid in regulating the behavioral trends of *** this study,we collected volatiles from *** at the seedling stage by using headspace solid phase microextraction(HS-SPME).Gas chromatography-mass spectrometry(GC-MS) analysis showed that approximately 11.03±6.00 and 238.25±121.67 ng hartemisinic acid were detected in volatile samples and milled samples,***,a key gene for artemisinic acid synthesis,the cytochrome P450 gene cyp71 av1,was expressed in engineered Saccharomyces cerevisiae to catalyze the production of artemisinic *** the addition of exogenous artemisinic alcohol or artemisinic aldehyde,artemisinic acid was identified as the product of the expressed *** electroantennogram(EAG) recordings,3-day-old adult *** showed significant electrophysiological responses to artemisinic alcohol,artemisinic aldehyde and artemisinic ***,3-day-old female bugs were significantly attracted by artemisinic acid and artemisinic alcohol at a concentration of 10 mmol L,whereas 3-day-old male bugs were attracted significantly by 10 mmol Lartemisinic acid and artemisinic *** propose that artemisinic acid and its precursors could be used as potential attractant components for the design of novel integrated pest management strategies to control ***.