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Proteomic profiling of cerebrospinal fluid in pediatric myelin oligodendrocyte glycoprotein antibody-associated disease

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World Journal of Pediatrics 2024年第3期20卷 259-271页

作者： Yi-Long Wang Meng-Ying Zhu Zhe-Feng Yuan Xiao-Yan Ren Xiao-Tong Guo Yi Hua Lu Xu Cong-Ying Zhao Li-Hua Jiang Xin Zhang Guo-Xia sheng Pei-fang Jiang Zheng-Yan Zhao Feng Gao Department of Neurology Children's HospitalZhejiang University School of MedicineHangzhou 310052China Children's Hospital Zhejiang University School of MedicineHangzhou 310052China Children's Hospital National Clinical Research Center for Child HealthZhejiang University School of MedicineHangzhou 310052China

Background Myelin oligodendrocyte glycoprotein(MOG)antibody-associated disease(MOGAD)is an autoimmune demy-elinating disorder of the central nervous *** Extracted proteins from 34 cerebrospinal fluid(CSF)samples[patients with MOGAD(MOG group,n=12);healthy controls(HC group,n=12);patients with MOG seronegative and metagenomics next-generation sequencing-negative inflammatory neurological diseases(IND group,n=10)]were processed and subjected to label-free quantitative *** partial least squares-discriminant analysis(PLS-DA)and orthogonal PLS-DA(O-PLS-DA)models were also performed based on proteomics *** analysis of differentially expressed proteins(DEPs)was performed using Gene Ontology,InterPro,and Kyoto Encyclopedia Genes and *** enzyme-linked immunosorbent assay was used to determine the complement levels in serum from patients with *** Four hundred and twenty-nine DEPs(149 upregulated and 280 downregulated proteins)were identified in the MOG group compared to the HC group according to the P value and fold change(FC).Using the O-PLS-DA model,872 differentially abundant proteins were identified with variable importance projection(VIP)scores>*** proteins(gamma-glutamyl hydrolase,cathepsin F,interalpha-trypsin inhibitor heavy chain 5,latent transforming growth factor beta-binding protein 4 and leukocyte-associated immunoglobulin-like receptor 1)overlapping between the top 30 DEPs with top-ranked P value and FC and top 30 proteins in PLS-DA VIP lists were *** analysis revealed that the dysregulated proteins in the MOG group were primarily involved in complement and coagulation cascades,cell adhesion,axon guidance,and glycosphingolipid biosynthesis compared to the HC *** The proteomic alterations in CSF samples from children with MOGAD identified in the current study might provide opportunities for developing novel biomarker candidates.

关键词： Acute disseminated encephalomyelitis Cerebrospinal fluid Complement cascades Myelin oligodendrocyte glycoprotein-associated disease Proteomics

Non-proteolytic ubiquitination of HBx controls HBV replication

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Virologica Sinica 2024年第2期39卷 338-342页

作者： Xiangpeng sheng Yi Yang Min Zhu Linlin Zhou fang Zhu Yuanfei Zhu Siying Dong Hui Kong Honghua Wang Ji Jiang Mingyue Wan Mingyang Feng Qiang Deng Yumin Xu Qing You Ronggui Hu State Key Laboratory for Animal Disease Control and Prevention Harbin Veterinary Research InstituteChinese Academy of Agricultural SciencesHarbin 150069China State Key Laboratory of Molecular Biology Shanghai Institute of Biochemistry and Cell BiologyCenter for Excellence in Molecular Cell ScienceChinese Academy of SciencesShanghai 200031China Key Laboratory of Systems Health Science of Zhejiang Province School of Life ScienceHangzhou Institute for Advanced StudyUniversity of Chinese Academy of SciencesHangzhou 310024China Department of Thoracic Surgery Ruijin HospitalShanghai Jiaotong University School of MedicineShanghai 200025China Shanghai Key Laboratory of Plant Molecular Sciences College of Life SciencesShanghai Normal UniversityShanghai 200234China Department of Pathogenic Biology West China School of Basic Medical Sciences&Forensic MedicineSichuan UniversityChengdu 610041China School of Medicine Guizhou UniversityGuiyang 550025China Key Laboratory of Medical Molecular Virology(MOE&MOH) School of Basic Medical SciencesFudan UniversityShanghai 200032China Department of Hospital Infection Management Department of Infectious DiseasesRuijin HospitalShanghai Jiao Tong University School of MedicineShanghai 200025China School of Biomedical Engineering Shanghai Jiao Tong UniversityShanghai 200240China

Dear Editor,Although effective vaccines and antiviral therapies are available,hepatitis B virus(HBV)infection is still a serious global health *** HBV infection remains the principal cause of liver cirrhosis and hepatocellular carcinoma(HCC).HBV is a small DNA virus,owning a~3.2 kb genome that encodes several proteins:viral DNA polymerase,core antigen(HBcAg),E antigen(HBeAg),three surface antigens(PreS1/PreS2/HBsAg),and a regulatory X protein(HBx)(Lamontagne et al.,2016).X protein,named for its lack of homology with any known proteins,is a 154 aa protein that plays an essential role in HBV biology and regulates the development of HCC(Yang et al.,2022).Although previous studies have strongly expanded our understanding of HBx,the regulation of HBx is not completely elucidated.

关键词： infection cirrhosis

Plexin domain-containing 1 may be a biomarker of poor prognosis in hepatocellular carcinoma patients,may mediate immune evasion

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World Journal of Gastrointestinal Oncology 2024年第5期16卷 2091-2112页

作者： Ming-Yue Tang Xue Shen Run-sheng Yuan Hui-Yuan Li Xin-Wei Li Yi-Ming Jing Yue Zhang Hong-Hong Shen Zi-Shu Wang Lei Zhou Yun-Chuan Yang He-Xin Wen fang Su Department of Medical Oncology The First Affiliated Hospital of Bengbu Medical CollegeBengbu 233000Anhui ProvinceChina Otolaryngology and Head and Neck Surgery The First Affiliated Hospital of Bengbu Medical CollegeBengbu 233000Anhui ProvinceChina Department of Neurology The First Affiliated Hospital of Bengbu Medical CollegeBengbu 233000Anhui ProvinceChina Department of Hepatobiliary Surgery The First Affiliated Hospital of Bengbu Medical CollegeBengbu 233000Anhui ProvinceChina Department of Gastrointestinal Surgery The First Affiliated Hospital of Bengbu Medical CollegeBengbu 233000Anhui ProvinceChina

BACKGROUND For the first time,we investigated the oncological role of plexin domain-containing 1(PLXDC1),also known as tumor endothelial marker 7(TEM7),in hepatocellular carcinoma(HCC).AIM To investigate the oncological profile of PLXDC1 in *** Based on The Cancer Genome Atlas database,we analyzed the expression of PLXDC1 in *** immunohistochemistry,quantitative real-time polymerase chain reaction(qRT-PCR),and Western blotting,we validated our *** prognostic value of PLXDC1 in HCC was analyzed by assessing its correlation with clinicopathological features,such as patient survival,methylation level,tumor immune microenvironment features,and immune cell surface checkpoint ***,to assess the immune evasion potential of PLXDC1 in HCC,we used the tumor immune dysfunction and exclusion(TIDE)website and immunohistochemical staining *** Based on immunohistochemistry,qRT-PCR,and Western blot assays,overexpression of PLXDC1 in HCC was associated with poor *** and multivariate Cox analyses indicated that PLXDC1 might be an independent prognostic *** HCC patients with high methylation levels,the prognosis was worse than in patients with low methylation *** enrichment analysis of HCC tissues indicated that genes upregulated in the high-PLXDC1 subgroup were enriched in mesenchymal and immune activation signaling,and TIDE assessment showed that the risk of immune evasion was significantly higher in the high-PLXDC1 subgroup compared to the low-PLXDC1 *** high-risk group had a significantly lower immune evasion rate as well as a poor prognosis,and PLXDC1-related risk scores were also associated with a poor *** As a result of this study analyzing PLXDC1 from multiple biological perspectives,it was revealed that it is a biomarker of poor prognosis for HCC patients,and that it plays a role in determining immune evasion status.

关键词： Plexin domain-containing 1 Biomarker Immune evasion Prognosis Immunotherapy Hepatocellular carcinoma

Integrated gut microbiota,plasma metabolomics and network pharmacology to reveal the anti-ischemic stroke effect of cooked rhubarb

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Traditional Medicine Research 2024年第9期9卷 1-13页

作者： Ting Li Lan-fang Tan Lin Shen Jie-Hui Kuang Si-Kai Zhan Bang Liu Jia-Le Cai sheng-Wang Liang Ming-Hua Xian Shu-Mei Wang School of Chinese Materia Medica Guangdong Pharmaceutical UniversityGuangzhou 510006China Key Laboratory of Digital Quality Evaluation of Chinese Materia Medica of State Administration of TCM School of Chinese Materia MedicaGuangdong Pharmaceutical UniversityGuangzhou 510006China Engineering&Technology Research Center for Chinese Materia Medica Quality of the Universities of Guangdong Province School of Chinese Materia MedicaGuangdong Pharmaceutical UniversityGuangzhou 510006China Guangxi Key Laboratory of Functional Phytochemicals Research and Utilization Guangxi Institute of BotanyChinese Academy of SciencesGuilin 541006China Traditional Chinese Medicine Resource Germplasm Bank Management Center Yunfu 527300China

Background:Cooked rhubarb(CR)is obtained by steaming raw rhubarb(Rheum palmatum L.,Rheum officinale Baill.,or Rheum tanguticum *** Balf.)with millet *** is used as a traditional Chinese medicine for treating cerebral stroke,where patients often face severe *** study explored the pharmacological effects and mechanisms of CR against ischemic stroke(IS)in *** used integrated analysis of gut microbiota,metabolomics,and network ***:The compounds in CR were identified using LC-MS/*** impact of CR on rat middle cerebral artery occlusion/reperfusion(MCAO/R)-induced cerebral infarct size and brain tissue pathology was assessed through 2,3,5-triphenyl tetrazolium chloride and HE *** in hemorheology were measured by evaluating blood viscosity,and serum levels of pro-inflammatory cytokine were also *** microbiota composition was analyzed through 16S rRNA gene *** metabolites were examined using untargeted metabolomics and Spearman correlation *** pseudo-germ-free test was used to determine whether tumour necrosis factor-α(TNF-α)and interleukin-1β(IL-1β)improvement in IS are dependent on gut ***:In vivo studies showed CR enhances neurobehavioral function in MCAO/R rats and reduces cerebral infarction *** also ameliorates brain and intestinal barrier damage caused by *** also decreased inflammation and oxidative stress in IS ***,CR promotes the growth of Akkermansia and Verrucomicrobia,aiding in intestinal barrier ***,CR primarily influences the primary bile acid biosynthesis *** pseudo-germ-free experiment and network pharmacology confirmed TNF-αand IL-1βas potential *** relies on gut microbiota for its anti-inflammatory effects to improve ***:Cooked rhubarb offers neuroprotective benefits by enhancing beneficial bacteria abundance and regulating bile acid *** emerges as a potential therapeutic agent for

关键词： cooked rhubarb ischemic stroke gut microbiota metabolomics

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落石撞击下双柱式RC梁桥的倒塌破坏分析

中国公路学报 2024年第5期37卷 94-107页

作者：吴昊肖方胜李瑞文同济大学土木工程学院上海200092 西安理工大学土木建筑工程学院陕西西安710048

针对山区梁桥面临落石撞击的威胁,利用LS-DYNA软件开展了落石撞击下典型双柱式RC梁桥倒塌破坏的精细化数值仿真分析。首先建立了落石撞击下考虑桩-土相互作用和钢筋-混凝土黏结滑移关系的原型梁桥有限元模型;其次基于双柱式RC桥墩水平... 详细信息

针对山区梁桥面临落石撞击的威胁,利用LS-DYNA软件开展了落石撞击下典型双柱式RC梁桥倒塌破坏的精细化数值仿真分析。首先建立了落石撞击下考虑桩-土相互作用和钢筋-混凝土黏结滑移关系的原型梁桥有限元模型;其次基于双柱式RC桥墩水平撞击试验,验证了选取的材料本构、网格尺寸、接触算法及其参数的可靠性;进一步模拟重现了汶川彻底关断桥事故,并分析了落石撞击下梁桥的倒塌过程;最后讨论了落石质量、撞击速度和高度对梁桥动态响应和损伤破坏的影响。结果表明:梁桥的倒塌破坏过程可依次分为被撞墩柱破坏、相邻墩柱破坏和上部结构坠落,其中被撞墩柱破坏是造成梁桥倒塌的主要因素;忽略桩-土相互作用和钢筋-混凝土黏结滑移关系造成墩柱截面响应预测结果偏小,高估梁桥的抗落石撞击性能;落石质量和撞击速度对被撞墩柱动态响应和梁桥整体损伤程度的影响显著,而撞击高度的影响相对较小。

关键词：桥梁工程落石撞击数值仿真双柱式RC梁桥动态响应

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BMFLC幅相补偿的舰船升沉测量方法

中国惯性技术学报 2024年第6期32卷 531-536,546页

作者：奔粤阳方时铮龚胜李倩哈尔滨工程大学智能科学与工程学院哈尔滨150001

在利用捷联式惯性导航系统进行升沉测量的过程中,引入数字高通滤波器可以很好地解决高度积分发散的问题,但随之会带来由高通滤波器固有特性导致的幅值衰减和相位超前。基于此,定量地分析了高通滤波器引起的幅相误差,提出一种基于带限傅... 详细信息

在利用捷联式惯性导航系统进行升沉测量的过程中,引入数字高通滤波器可以很好地解决高度积分发散的问题,但随之会带来由高通滤波器固有特性导致的幅值衰减和相位超前。基于此,定量地分析了高通滤波器引起的幅相误差,提出一种基于带限傅里叶线性组合(BMFLC)幅相补偿的升沉测量方法,并在BMFLC中引入遗忘卡尔曼滤波权值更新策略。在提高BMFLC拟合精度的基础上,进一步提高对数字高通滤波器输出信息的幅相误差补偿精度。最后利用六自由度运动平台和舰船实验验证所提方法,实验结果表明:所提方法测得的升沉位移均方根误差约为8.1 mm,具有较高的适用性和稳定性。

关键词：升沉测量带限傅里叶线性组合数字高通滤波器

存在未知时滞非线性系统的迭代变区间预测迭代学习控制

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控制理论与应用 2024年第4期41卷 701-715页

作者：余琼霞田丰臣孙俊杰侯忠生河南理工大学电气工程与自动化学院河南焦作454000 焦作煤业(集团)有限责任公司铁路运输处河南焦作454000 青岛大学自动化学院山东青岛266071

本文针对机理模型未知的非线性非仿射多入多出(MIMO)离散时间系统,研究了系统同时存在未知时滞和迭代变化运行时间区间的预测迭代学习控制(PILC)问题.首先利用未知时滞的上下界信息建立了一种新型的动态线性化(DL)模型,理论分析表明该... 详细信息

本文针对机理模型未知的非线性非仿射多入多出(MIMO)离散时间系统,研究了系统同时存在未知时滞和迭代变化运行时间区间的预测迭代学习控制(PILC)问题.首先利用未知时滞的上下界信息建立了一种新型的动态线性化(DL)模型,理论分析表明该模型能够等价描述本文所考虑的存在未知时滞的未知非线性系统.同时,设计一种新的数据补偿机制用以处理由于系统运行时间区间迭代变化而引起的数据丢失问题.基于所建立的DL模型和数据补偿机制,设计了能够同时处理未知时滞和迭代变化运行时间区间的预测迭代学习控制方法.通过严格的理论分析同时给出了建模误差和跟踪控制误差的收敛性质.最后,通过仿真进一步验证了所提方法的有效性.

关键词：迭代学习控制预测迭代学习控制未知时滞迭代变区间

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青藏高原不同植被亚型的光谱特征分析

光谱学与光谱分析 2024年第9期44卷 2638-2646页

作者：牛洁琼任鸿瑞周广胜太原理工大学测绘科学与技术系山西太原030024 中国气象科学研究院北京100081

光谱曲线是植被生理性状的重要表征,研究植被光谱对监测植被的生长状况和健康状态具有重要意义。当前植被光谱研究多对同一植被型内不同植被进行分析,鲜有系统地整合不同植被亚型光谱特征的报道。近年来青藏高原的生态格局发生了重大变... 详细信息

光谱曲线是植被生理性状的重要表征,研究植被光谱对监测植被的生长状况和健康状态具有重要意义。当前植被光谱研究多对同一植被型内不同植被进行分析,鲜有系统地整合不同植被亚型光谱特征的报道。近年来青藏高原的生态格局发生了重大变化,为实现基于青藏高原大范围尺度的植被光谱特征研究工作,探讨不同植被亚型的光谱可分离性,基于高分辨率Sentinel-2A/B遥感影像,通过一阶微分光谱和植被指数对青藏高原19种植被亚型的光谱曲线进行分析。结果表明:(1)植被亚型的光谱曲线在近红外波段表现出明显差异,其反射率值可用于区分不同植被亚型;(2)一阶微分处理能够明确植被三边特征,植被的一阶微分光谱具有规律性,在红边处差异显著;(3)不同植被亚型的植被指数与红边参数变化相一致,植被指数虽然数值偏小但具有区分性;(4)综合运用光谱反射率值、三边参数和植被指数,可区分不同植被亚型。在植被亚型的尺度上分析了不同植被的光谱曲线特征,实验结果可为植被亚型光谱数据库的完善提供数据支撑,对青藏高原植被亚型精细识别工作具有实际意义。

关键词：青藏高原植被亚型光谱特征一阶微分三边特征参数

Investigating the Immunogenic Cell Death‑Dependent Subtypes and Prognostic Signature of Triple‑Negative Breast Cancer

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Phenomics 2024年第1期4卷 34-45页

作者： Youyang Shi Yuanyuan Wu Feifei Li Kexin Jiang Xiaofang fang Yu Wang Xiaoyun Song Rui Wang Lixin Chen Jinzhou Zheng Chunyu Wu Yuenong Qin Xiaofei Liu sheng Liu Breast Surgery Longhua HospitalShanghai University of Traditional Chinese MedicineWanping South Road725Shanghai 200033China First Clinical College Shandong University of Traditional Chinese MedicineNo.16369Jingshi RoadLixia DistrictJinan 250011ShandongChina

Recently,immunotherapy has emerged as a promising and efective method for treating triple-negative breast cancer(TNBC).However,challenges still *** cell death(ICD)is considered a prospective treatment and potential combinational treatment strategy as it induces an anti-tumor immune response by presenting the antigenic epitopes of dead ***,the ICD process in TNBC and its impact on disease progression and the response to immunotherapy are not well *** this study,we observed dysregulation of the ICD process and verifed the altered expression of prognostic ICD genes in TNBC through quantitative real-time polymerase chain reaction(qRT-PCR)*** investigate the potential role of the ICD process in TNBC progression,we determined the ICD-dependent subtypes,and two were *** of their distinct tumor immune microenvironment(TIME)and cancer hallmark features revealed that Cluster 1 and 2 corresponded to the immune“cold”and“hot”phenotypes,*** addition,we constructed the prognostic signature ICD score of TNBC patients and demonstrated its clinical independence and *** ICD score could also serve as a potential biomarker for immune checkpoint blockade and may aid in the identifcation of targeted efective agents for individualized clinical strategies.

关键词： Immunogenic cell death Triple-negative breast cancer Tumor immune microenvironment Prognostic signature