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Journal of Integrative Agriculture 2020年第4期19卷 1033-1043页

作者： GAO Yue-rong SUN Jia-chen SUN Zhi-lin XING Yu ZHANG Qing fang ke-feng CAO Qing-qin QIN Ling Beijing Advanced Innovation Center for Tree Breeding by Molecular Design Beijing University of AgricultureBeijing 102206P.R.China Beijing Bei Nong Enterprise Management Co. Ltd.Beijing 102206P.R.China College of Plant Science and Technology Beijing Key Laboratory for Agricultural Application and New TechniqueBeijing University of AgricultureBeijing 102206P.R.China Beijing Collaborative Innovation Center for Eco-environmental Improvement with Forestry and Fruit Trees Beijing University of AgricultureBeijing 102206P.R.China

Somatic embryogenesis(SE)is an effective approach of in vitro regeneration that depends on plant cell ***,largely unknown of molecular mechanisms of SE in woody plants such as Chinese chestnut(Castanea mollissima Blume),limits the development of the woody plant ***,we report the MADS-box transcription factor Cm AGL11 in Chinese *** AGL11 transcripts specifically accumulated in the globular *** of Cm AGL11 in chestnut callus enhanced its SE capacity,and the development of somatic embryos occurred significantly faster than in the ***-seq results showed that Cm AGL11 affects the expression of several genes related to the gibberellin,auxin,and ethylene ***,the analysis of DNA methylation status indicated that the promoter methylation plays a role in regulation of Cm AGL11 expression during *** results demonstrated that Cm AGL11 plays an important role in the SE process in Chinese chestnut,possibly by regulating gibberellin,auxin,and ethylene *** will help establish an efficient platform to accelerate genetic improvement and germplasm innovation in Chinese chestnut.

关键词： Chinese chestnut CmAGL11 somatic embryogenesis DNA methylation

Fingerprinting 146 Chinese chestnut(Castanea mollissima Blume)accessions and selecting a core collection using SSR markers

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Journal of Integrative Agriculture 2021年第5期20卷 1277-1286页

作者： NIE Xing-hua WANG Ze-hua LIU Ning-wei SONG Li YAN Bo-qian XING Yu ZHANG Qing fang ke-feng ZHAO Yong-lian CHEN Xin WANG Guang-peng QIN Ling CAO Qing-qin Beijing Advanced Innovation Center for Tree Breeding by Molecular Design Beijing University of AgricultureBeijing 102206P.R.China College of Plant Science and Technology Beijing Key Laboratory for Agricultural Application and New TechniqueBeijing University of AgricultureBeijing 102206P.R.China College of Landscape Architecture Beijing University of AgricultureBeijing 102206P.R.China Chestnut Technology Experiment and Extension Station of Huairou District Beijing 102206P.R.China Shandong Institute of Pomology Shandong Academy of Agricultural SciencesTai’an 271000P.R.China Changli Institute of Pomology Hebei Academy of Agriculture and Forestry SciencesChangli 066600P.R.China

Chinese chestnut is an important nut tree around the *** the types of Chinese chestnut resources are abundant,resource utilization and protection of chestnut accessions are still very ***,we fingerprinted and determined the genetic relationships and core collections of Chinese chestnuts using 18 fluorescently labeled SSR markers generated from 146 chestnut *** analyses showed that these markers from the tested accessions are highly polymorphic,with an average allele number(N_(a))and polymorphic information content(PIC)of 8.100 and 0.622 per locus,*** these strongly distinguishing markers,we successfully constructed unique fingerprints for 146 chestnut accessions and selected seven of the SSR markers as core markers to rapidly distinguish different *** exploration of the genetic relationships among the five cultivar groups indicated that Chinese chestnut accessions are divided into three regional type groups:group I(North China(NC)and Northwest China(NWC)cultivar groups),group II(middle and lower reaches of the Yangtze River(MLY)cultivar group)and group III(Southeast China(SEC)and Southwest China(SWC)cultivar groups).Finally,we selected 45 core collection members which represent the most genetic diversity of Chinese chestnut *** study provides valuable information for identifying chestnut accessions and understanding the phylogenetic relationships among cultivar groups,which can serve as the basis for efficient breeding in the future.

关键词： Castanea mollissima SSR markers fingerprinting phylogeny core collections

Highly mass activity electrocatalysts with ultralow Pt loading on carbon black for hydrogen evolution reaction

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International Journal of Minerals,Metallurgy and Materials 2025年第1期32卷 182-190页

作者： Shaorou ke Yajing Zhao Xin Min Yanghong Li Ruiyu Mi Yangai Liu Xiaowen Wu Minghao fang Zhaohui Huang Engineering Research Center of Ministry of Education for Geological Carbon Storage and Low Carbon Utilization of Resources Beijing Key Laboratory of Materials Utilization of Nonmetallic Minerals and Solid WastersNational Laboratory of Mineral MaterialsSchool of Materials Science and TechnologyChina University of Geosciences(Beijing)Beijing 100083China

Pt-based nanocatalysts offer excellent prospects for various ***,the low loading of Pt with excellent performance for efficient and stable nanocatalysts still presents a considerable *** this study,nanocatalysts with ultralow Pt content,excellent performance,and carbon black as support were prepared through in-situ ***~2-nm particles uniformly and stably dispersed on carbon black because of the strong s-p-d orbital hybridizations between carbon black and Pt,which suppressed the agglomeration of Pt *** unique structure is beneficial for the hydrogen evolution *** catalysts exhibited remarkable catalytic activity for hydrogen evolution reaction,exhibiting a potential of 100 mV at 100 mA·cm^(-2),which is comparable to those of commercial Pt/C *** activity(1.61 A/mg)was four times that of a commercial Pt/C catalyst(0.37 A/mg).The ultralow Pt loading(6.84wt%)paves the way for the development of next-generation electrocatalysts.

关键词： hydrogen evolution reaction ultralow platinum in-situ synthesis ultrasound

EGTA对‘秦冠’苹果花粉管壁主要成分的影响

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电子显微学报 2020年第2期39卷 181-188页

作者：李田张卿曹庆芹秦岭房克凤北京农学院园林学院北京102206 北京农学院植物科学技术学院/农业应用新技术北京市重点实验室北京102206

本文以不同浓度Ca^2+螯合剂EGTA处理‘秦冠’苹果成熟花粉,观察其对花粉管细胞壁主要成分的影响。在低浓度EGTA(400μmol·L^-1EGTA)处理下纤维素、胼胝质、酯化果胶无明显变化,酸性果胶、阿拉伯半乳糖蛋白在花粉管壁两侧的荧光强度增... 详细信息

本文以不同浓度Ca^2+螯合剂EGTA处理‘秦冠’苹果成熟花粉,观察其对花粉管细胞壁主要成分的影响。在低浓度EGTA(400μmol·L^-1EGTA)处理下纤维素、胼胝质、酯化果胶无明显变化,酸性果胶、阿拉伯半乳糖蛋白在花粉管壁两侧的荧光强度增强。在高浓度EGTA(2000μmol·L^-1 EGTA)处理下苹果花粉管上的纤维素无明显变化;胼胝质在花粉管顶端富集;花粉管顶端及两侧壁酸性果胶、酯化果胶浓度增加;花粉管壁两侧及基部的阿拉伯半乳糖蛋白荧光强度增加。进一步用傅立叶红外光谱技术分析验证了花粉管顶端细胞壁化学成分的变化。因此作者推测EGTA通过干扰钙的含量进而调控细胞壁多糖成分,进而造成细胞壁的物理机械强度以及延展性的变化,最终达到调节花粉萌发及花粉管生长的作用。

关键词：苹果 EGTA 花粉管细胞壁

FT-IR光谱法对酒炖熟地黄炮制工艺过程的监控研究

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中成药 2005年第5期27卷 544-549页

作者：白雁孙素琴李军樊克锋王东陈志红河南中医学院河南郑州450008 清华大学北京100084 郑州牧业工程高等专科学校河南郑州450011

目的:跟踪熟地炮制工艺全过程,寻求熟地黄最佳“炮制终点”,规范熟地黄饮片质量标准。方法:利用FT IR红外光谱法,从分子水平,快速、准确,整体宏观监控炮制过程,分析生地黄特征峰和熟地黄特征峰变化趋势和规律。结果:用直观的量化的标准... 详细信息

目的:跟踪熟地炮制工艺全过程,寻求熟地黄最佳“炮制终点”,规范熟地黄饮片质量标准。方法:利用FT IR红外光谱法,从分子水平,快速、准确,整体宏观监控炮制过程,分析生地黄特征峰和熟地黄特征峰变化趋势和规律。结果:用直观的量化的标准红外光谱谱图来说明“黑如漆,甜如饴”这种传统的经验标准。结论:在地黄传统炮制经验基础上,规范其炮制工艺,制定其饮片质量标准,并为中药炮制质量检测、工艺监控等提供强有力分析手段。

关键词： FTIR 熟地黄炮制终点

北方四种卫矛属常绿阔叶植物叶肉细胞超微结构在冬季的适应性变化

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电子显微学报 2010年第2期29卷 167-172页

作者：葛秀秀房克凤郝强于建军关雪莲北京农学院生物技术系北京102206 北京农学院农业应用新技术北京市重点实验室北京102206 北京农学院园林系北京102206

以北京地区生长的4种常绿阔叶植物北海道黄杨(Euonymus japonicus cv.'CuZhi')、大叶黄杨(*** Thunb.)、金心黄杨(*** ***-pictus)和扶芳藤[***(Turcz.)hand.-Mazz]为材料,研究其叶肉细胞超微结构在冬季应答低温胁迫的适应性变化。结果... 详细信息

以北京地区生长的4种常绿阔叶植物北海道黄杨(Euonymus japonicus cv.'CuZhi')、大叶黄杨(*** Thunb.)、金心黄杨(*** ***-pictus)和扶芳藤[***(Turcz.)hand.-Mazz]为材料,研究其叶肉细胞超微结构在冬季应答低温胁迫的适应性变化。结果表明:在冬季北海道黄杨、大叶黄杨和金心黄杨叶肉细胞的叶绿体结构相对完整,其中的嗜锇颗粒增多,淀粉粒消失;叶绿体周围的线粒体数量显著增多,且结构完整、内嵴明显;细胞质中有同心圆状排列的多层膜结构出现。扶芳藤在初冬时叶肉细胞中的叶绿体有些肿胀,内膜系统稀疏,淀粉粒消失;随着气温进一步的降低,叶绿体出现解体,这些特征表明扶芳藤的抗寒性弱于其它3种常绿阔叶植物。

关键词：卫矛属常绿阔叶植物叶肉细胞超微结构

外源硼和钙对“索邦”百合花粉萌发和花粉管生长的影响

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电子显微学报 2014年第4期33卷 368-372页

作者：杨瑞高赛王金金秦岭房克凤农业应用新技术北京市重点实验室北京农学院植物科学技术学院北京102206

本文采用花粉液体培养法研究硼、钙离子对"索邦"百合花粉萌发和花粉管生长的影响。结果表明:百合花粉萌发和花粉管生长需要适宜的外源硼酸,适宜浓度为1mmol/L,低浓度促进花粉萌发和花粉管生长,而浓度过高则起抑制作用。适量的外源钙离... 详细信息

本文采用花粉液体培养法研究硼、钙离子对"索邦"百合花粉萌发和花粉管生长的影响。结果表明:百合花粉萌发和花粉管生长需要适宜的外源硼酸,适宜浓度为1mmol/L,低浓度促进花粉萌发和花粉管生长,而浓度过高则起抑制作用。适量的外源钙离子有利于花粉萌发和花粉管生长,适宜浓度为1mmol/L,高离子浓度抑制花粉萌发和花粉管生长。

关键词：百合硼钙花粉萌发花粉管生长

Cohort study on the treatment of BRAF V600E mutant metastatic colorectal cancer with integrated Chinese and western medicine

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World Journal of Clinical Oncology 2025年第1期16卷 25-33页

作者： Jiang-Yu Bian Yu-fang feng Wen-Ting He Tong Zhang Department of Oncology Xiyuan Hospital of China Academy of Chinese Medical SciencesBeijing 100091China Department of Oncology The Fourth Clinical Medical College of Xinjiang Medical UniversityUrumqi 830001China Department of Oncology Traditional Chinese Medicine Hospital Affiliated to Xinjiang Medical UniversityUrumqi 830001China

BACKGROUND Patients with BRAF V600E mutant metastatic colorectal cancer(mCRC)have a low incidence rate,poor biological activity,suboptimal response to conventional treatments,and a poor *** the previous cohort study on mCRC conducted by our team,it was observed that integrated Chinese and Western medicine treatment could significantly prolong the overall survival(OS)of patients with colorectal ***,we further explored the survival benefits in the population with BRAF V600E mutant *** To evaluate the efficacy of integrated Chinese and Western medicine in the treatment of BRAF V600E mutant metastatic colorectal *** A cohort study was conducted on patients with BRAF V600E mutant metastatic colorectal cancer admitted to Xiyuan Hospital of China Academy of Chinese Medical Sciences and Traditional Chinese Medicine Hospital of Xinjiang Uygur Autonomous Region from January 2016 to December *** patients were divided into two *** A total of 34 cases were included,with 23 in Chinese-Western medicine cohort(cohort A)and 11 in Western medicine cohort(cohort B).The median overall survival was 19.9 months in cohort A and 14.2 months in cohort B,with a statistically significant difference(P=0.038,hazard ratio=0.46).The 1-3-year survival rates were 95.65%(22/23),39.13%(9/23),and 26.09%(6/23)in cohort A,and 63.64%(7/11),18.18%(2/11),and 9.09%(1/11)in cohort B,*** analysis showed statistically significant differences in median OS between the two cohorts in the right colon,liver metastasis,chemotherapy,and first-line treatment subgroups(P<0.05).CONCLUSION Integrated Chinese and Western medicine can prolong the survival and reduce the risk of death in patients with BRAF V600E mutant metastatic colorectal cancer,with more pronounced benefits observed in patients with right colon involvement,liver metastasis,combined chemotherapy,and first-line treatment.

关键词： Metastatic colorectal cancer BRAF V600E mutation Integrated Chinese and Western medicine Cohort study

森林草莓独脚金内酯合成关键基因D27的克隆与表达分析

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园艺学报 2016年第5期43卷 975-982页

作者：赵晨晨范雅丽秦岭邢宇房克凤张卿曹庆芹北京农学院植物科学与技术学院农业应用新技术北京市重点实验室北京102206 北京农学院生物科学与工程学院北京102206 北京林果业生态环境功能提升协同创新中心北京102206

根据森林草莓中独角金内酯合成过程中的关键基因D27的序列信息设计引物,获得了D27基因的完整开放阅读框架。采用MEGA5.0软件,对森林草莓与其他物种D27基因编码的氨基酸序列进行聚类分析,发现森林草莓D27基因与其他物种D27基因具有较高... 详细信息

根据森林草莓中独角金内酯合成过程中的关键基因D27的序列信息设计引物,获得了D27基因的完整开放阅读框架。采用MEGA5.0软件,对森林草莓与其他物种D27基因编码的氨基酸序列进行聚类分析,发现森林草莓D27基因与其他物种D27基因具有较高的同源性。设置正常供磷、缺磷和在缺磷条件下接种丛枝状菌根真菌处理,利用电感耦合等离子发射光谱仪测定草莓植株的磷含量,结果表明缺磷条件下接种丛枝状菌根真菌,草莓植株中磷含量极显著提高,而缺磷处理的磷含量极显著降低。实时荧光定量PCR结果表明,与正常供磷处理相比,缺磷和接种丛枝状菌根真菌处理条件下,D27基因的表达量分别上调7倍和11倍,这说明D27基因的表达既受到磷水平调控,又受到菌根形成的调控。

关键词：森林草莓 D27基因聚类树磷含量表达分析

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钙离子对青杄花粉萌发和花粉管生长的影响

电子显微学报 2018年第6期37卷 627-636页

作者：杜兵帅胡海文曹庆芹秦岭房克凤北京农学院园林学院北京102206 北京市昌平区流村镇人民政府北京102204 北京农学院植物科学技术学院/农业应用新技术北京市重点实验室北京102206 北京林果业生态环境功能提升协同创新中心北京102206

本文以成熟的青杄花粉为试验材料,用不同浓度钙拮抗剂EGTA和抑制剂AlCl_3进行处理,观察花粉的萌发和花粉管的生长情况,同时用选择性电极测定了不同发育时间内花粉管顶端钙离子流,以及EGTA和AlCl_3对钙离子流的影响,以探讨钙对青杄花粉... 详细信息

本文以成熟的青杄花粉为试验材料,用不同浓度钙拮抗剂EGTA和抑制剂AlCl_3进行处理,观察花粉的萌发和花粉管的生长情况,同时用选择性电极测定了不同发育时间内花粉管顶端钙离子流,以及EGTA和AlCl_3对钙离子流的影响,以探讨钙对青杄花粉生长发育的调控机制。结果发现:(1) 0.5 mmol·L^(-1) EGTA处理花粉后,会促进花粉萌发,萌发率升高;但花粉管长度低于对照组;1 mmol·L^(-1) EGTA处理后,花粉萌发率和花粉管长度都低于对照组;0.1 mmol·L^(-1)和0.5 mmol·L^(-1) AlCl_3处理后,花粉萌发率和花粉管长度都会显著降低;(2)通过非损伤微测技术发现,对照花粉在水合时期(培养3,6,9 h)钙离子外流,在萌发及快速伸长期钙离子内流(培养12,15,18 h);EGTA和AlCl_3培养花粉后,花粉的水合期、萌发期和花粉管的伸长期的钙流状况会出现异常,其中包括钙流的内流和外流的状况以及流速,从而导致各个时期花粉的萌发率及和花粉管长度的改变,导致花粉管的生长异常。(3)对照花粉管顶端出现Ca^(2+)浓度梯度,而0.5 mmol·L^(-1) EGTA处理的花粉会相比对照组提前在花粉管顶端出现Ca^(2+)浓度梯度,以促进花粉萌发;1 mmol·L^(-1 )EGTA,0.1 mmol·L^(-1) AlCl_3和0.5 mmol·L^(-1) AlCl_3处理后Ca^(2+)浓度梯度消失。由此表明花粉的不同生长时期对钙离子的需求不同,且钙离子对花粉管的萌发及生长起着至关重要的作用。

关键词：青杄花粉萌发花粉管生长花粉管 Ca^2+