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STUDYING THE ROLE OF MACROPHAGES IN CIRCULATING PROSTATE CANCER CELLS BY IN VIVO FLOW CYTOMETRY

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Journal of Innovative Optical Health Sciences 2012年第4期5卷 39-47页

作者： JIN GUO zhichao fan ZHENGQIN GU XUNBIN WEI Department of Chemistry Fudan UniversityShanghaiP.R.China Institutes of Biomedical Sciences Fudan UniversityShanghaiP.R.China Med-X Research Institute Shanghai Jiao Tong University 1954Huashan RoadShanghai 200240P.R.China School of Biomedical Engineering Shanghai Jiao Tong University ShanghaiP.R.China Department of Urology Xinhua Hospital Shanghai Jiao Tong University 1665Kongjiang RoadShanghai 200092P.R.China

Metastasis is a very complicated multi-step process and accounts for the low survival rate of the cancerous *** metastasize,t he malignant cells must detach from the primary tumor and migrate to secondary sites in the body through either blood or lymph *** appear to be directly involved in tumor progression and ***,the role of macrophages in affecting cancer metast asis has not been fully ***,we have utilized an emerging technique,namely in vivo flow cytometry(IVFC)to study the depletion kinetics of circulating prostate cancer cells in mice and determine how depletion of macrophages by the liposome encapsulated clodronate affects the depletion *** results show diferent depletion kinetics of PC-3 cells between the macrophagedeficient group and the control *** number of circulating tumor cells(CTCs)in the macrophage-deficient group decreases in a slower manner compared to the control mice *** differences in depletion kinetics indicate that the absence of macrophages facilitates the stay of prostate cancer cells in *** addition,our imaging data suggest that macrophages might be able to arrest,phagocytose and digest PC-3 ***,phagocy tosis may mainly contribute to the de-pletion kinetic *** developed methods elaborated here would be useful to study the relationship between macr ophages and tumor metastasis in small animal cancer models.

关键词： Prostate cancer macrophages liposome-encapsulated clodronate in vrivo flow cytometer circulating tumor cells

Creep-fatigue lives prediction and sensitivity study of 316H at 550 ℃

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Acta Metallurgica Sinica(English Letters) 2011年第2期24卷 132-140页

作者： zhichao fan David J Smith Xuedong CHEN Mike W Spindler Hefei General Machinery Research Institute Hefei 23-0031 China University of Bristol Bristol BS8 1TR UK British Energy Gloucester GL4 3RS UK

In Volume 2/3 of R5 ＂An assessment procedure for the high temperature response of structures＂, the strain based ductility exhaustion method is suggested to calculate the creep damage of stress concentration region, which involves description of creep dwell initial stress, stress drop and creep ductility. Considering lots of uncertainty existed in these assessment, some sensitivity analysis is required in R5 procedure to ensure the conservatism of assessment results. In this paper, laboratory creep-fatigue test data of 316H at 550 ℃ with different loading conditions are selected as a special case to investigate whether the basic R5 approach is conservative, and the different material data combinations of cyclic stress-strain, creep deformation and creep ductility are used to identify those significant parameters affecting the assessment results. The analytical results indicate that the creep deformation model and creep ductility data are more significant to the results comparing with cyclic stress-strain data. If the upper bound of creep deformation law and lower bound of creep ductility data are used to predict their creep-fatigue lives, the degree of conservatism can be as large as a factor of -300, but if the modified creep deformation model and cast specific ductility data are used, very well prediction results can be gained within a factor of ±2.0 although there is slight non-conservatism.

关键词： Creep Fatigue Damage Life prediction Sensitivity study

Modeling and Experimental Analysis of Roughness Effect on Ultrasonic Nondestructive Evaluation of Micro-crack

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Chinese Journal of Mechanical Engineering 2021年第6期34卷 126-137页

作者： Zhe Wang zhichao fan Xuedong Chen Yihua Kang Jingwei Cheng Wei Chen National Safety Engineering Technology Research Center for Pressure Vessels and Pipeline Hefei General Machinery Research Institute Co.LtdHefei230031China State Key Lab of Digital Manufacturing Equipment&Technology Huazhong University of Science and TechnologyWuhan430074China

A high-precision evaluation of ultrasonic detection sensitivity for a micro-crack can be restricted by a corroded rough surface when the surface microtopography is of the same order of magnitude as the crack *** this study,a back-surface micro-crack is considered as a research target.A roughness-modified ultrasonic testing model for micro-cracks is established based on a multi-Gaussian beam model and the principle of phase-screen *** echo signals of micro-cracks and noises corresponding to different rough front surfaces and rough back surfaces are obtained based on a reference reflector signal acquired from a two-dimensional simulation *** compari-son between the analytical and numerical models shows that the responses of micro-cracks under the effects of dif-ferent corroded rough surfaces can be accurately *** numerical and analytical results show that the echo signal amplitude of the micro-crack decreases significantly with an increase in roughness,whereas the noise ampli-tude slightly ***,the effect of the rough front surface on the echo signal of the micro-crack is greater than that of the rough back *** the root-mean-square(RMS)height of the surface microtopography is less than 15μm,the two rough surfaces have less influence on the echo signals detected by a focused transducer with a frequency of 5 MHz and diameter of 6 mm.A method for predicting and evaluating the detection accuracy of micro-cracks under different rough surfaces is proposed by combining the theoretical model and a finite element ***,a series of rough surface samples containing different micro-cracks are fabricated to experimentally validate the evaluation method.

关键词： Surface roughness Micro-crack Ultrasonic testing model Detection accuracy Evaluation method

Visualization of integrin molecules by fluorescence imaging and techniques

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BIOCELL 2021年第2期45卷 229-257页

作者： CHEN CAI HAO SUN LIANG HU zhichao fan Department of Immunology School of MedicineUConn HealthFarmington06030USA Department of Medicine University of CaliforniaSan DiegoLa Jolla92093USA Cardiovascular Institute of Zhengzhou University Department of CardiologyThe First Affiliated Hospital of Zhengzhou UniversityZhengzhou450051China

Integrin molecules are transmembraneαβheterodimers involved in cell adhesion,trafficking,and *** activation,integrins undergo dynamic conformational changes that regulate their affinity to *** physiological functions and activation mechanisms of integrins have been heavily discussed in previous studies and reviews,but the fluorescence imaging techniques-which are powerful tools for biological studies-have *** we review the fluorescence labeling methods,imaging techniques,as well as Förster resonance energy transfer assays used to study integrin expression,localization,activation,and functions.

关键词： Integrins Fluorescence imaging Fluorescence labeling Live-cell imaging Super-resolution imaging Intravital imaging FRET

The pitch-catch nonlinear ultrasonic imaging techniques for structural health monitoring

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Science China(Technological Sciences) 2021年第12期64卷 2608-2617页

作者： CHENG JingWei DRINKWATER Bruce W. CHEN XueDong fan zhichao CHEN Wei WANG Zhe National Safety Engineering Technology Research Centre for Pressure Vessels and Pipelines Hefei General Machinery Research Institute Co.Ltd.Hefei 230031China Department of Mechanical Engineering University of BristolBristol BS81TRUnited Kingdom

Nonlinear ultrasonic imaging techniques in pulse-echo configuration have recently shown their potential to allow the effective separation of nonlinear and linear features in a nonlinear *** this study,two ultrasonic phased arrays are implemented to produce an image of elastic nonlinearity through the parallel-sequential subtraction of the coherently scattered components in the through-transmission acoustic field at the transmission or subharmonic *** parallel mode,a physical focus at each pixel is achieved by firing the transmitters with a predefined delay *** sequential mode,each transmitter is fired in sequence and all the receivers are employed to capture the data *** full matrix captured data can be post-processed and focused synthetically at the target *** images of parallel focusing and sequential focusing are expected to be linearly identical and hence any differences remained on the subtracted image can be related to the nonlinearities arising from the ***,the imaging metric here is defined as the difference between parallel and sequentially focused amplitudes obtained from forward coherently scattered fields at each target ***,the negative influences due to the instrumentation nonlinearities are investigated by studying the remaining relative phase and amplitude at undamaged pixels.A compensation method is implemented to suppress these noises,significantly enhancing the selectivity of nonlinear scattering *** proposed techniques are then implemented to monitor fatigue crack growth in order to explore the capability of these methods as measures of elastic nonlinearity induced by different sizes of small closed *** promising results suggest that nonlinear imaging can be used to monitor crack growth and improve the detectability at early stages.

关键词： pitch-catch coherent measurement nonlinear imaging fatigue crack monitoring ultrasonic phased array nonlinearities decoupling

Design,Manufacture and Maintenance of Pressure Equipment Based on Accidents Survey

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Chinese Journal of Mechanical Engineering 2011年第2期24卷 167-178页

作者： CHEN Xuedong AI Zhibin fan zhichao WANG Bing DOU Wanbo YANG Tiecheng GUAN Weihe National Technical Research Center on Safety Engineering of Pressure Vessels and Pipelines Hefei General Machinery Research Institute Hefei 230031 China

Most of the important units of pressure equipment have been manufactured successfully in China related to the national key construction projects,such as 10 million tons/year oil refinery,million tons/year ethylene,large coal chemical,***,some of them failed to operate shortly after their putting into *** suffered severe damage even during the previous period of manufacture and *** this paper,cases of accident survey and failure analysis are given for some typical pressure *** is found that many accidents are related to insufficient consideration of the design and manufacture of the *** accidents occur fundamentally because of the Chinese design standards codes for pressure equipment without risk or life concepts and the support from a database for potential risk existing in their dynamic *** designers and manufacturers are unable to make correct design,materials selection and manufacturing process all due to a lack of engineering *** order to avoid the repetition of the accidents and improve the safety,reliability and economy of pressure equipment,a platform is suggested for design,manufacture and maintenance of pressure equipment in China based on accidents *** other words,some effective precautionary measures are taken at the design and manufacture stage,and the design methodology has to be based on service life requirement and desirable risk *** the service stage some reasonable inspection/monitoring approaches should be utilized to control risks and ensure the equipment operating safely until its desired ***,the basic scheme and some key technologies are briefly given for the platform *** concept of risk and life based design,manufacture and maintenance proposed herein has important significance for improving and perfecting the codes and standards for design,manufacture and maintenance of Chinese pressure-bearing equipment,enhancing the life and reliability of

关键词： pressure vessel pressure piping damage risk design manufacture maintenance

Toluidine blue O and porphyrin-mediated photodynamic therapy on three main pathogenic bacteria of periodontitis using portable LED phototherapy device

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Journal of Innovative Optical Health Sciences 2015年第4期8卷 32-38页

作者： Xuewei Jiang zhichao fan Yili Yu Chenying Shao Yuanzhen Suo Xunbin Wei Yi Zhou Department of Stomatology Huashan HospitalFudan University ShanghaiP.R.China Institutes of Biomedical Sciences Fudan UniversityShanghaiP.R.China Department of Chemistry Fudan UniversityShanghaiP.R.China Med-X Research Institute Shanghai Jiao Tong University ShanghaiP.R.China School of Biomedical Engineering Shanghai Jiao Tong University ShanghaiP.R.China Shanghai Stomatological Disease Center ShanghaiP.R.China Division of Inflammation Biology La Jolla Institute for Allergy&ImmunologyLa JollaCaliforniaUSA

Photodynamic therapy(PDT)has been commonly used in treating many diseases,such as cancer and infectious *** investigated the different effects of PDT on three main pathogenic bacteria of periodontitis-Prevotella melaninogenica(P.m.),Porphyromonas gingitvalis(P.g.)and Aggregatibacter actinomycetercomitans(A.a-).The portable red light-ermitting diode(LED)phototherapy device was used to assess the exogenous PDT effects with different light doses and photosensitizer concentrations(Toluidine blue O,TBO).The portable blue LED phototherapy device was used to assess the endogenous PDT effects with the use of endogenous photosensit izers(porphyrin)under dfferent light *** found out that both exogenous and endogenous PDT were able to restrict the growth of all the three bacteria ***,the optimal PDT conditions for these bacteria were obtained through this in vitro screening and could guide the clinical PDT on periodontitis.

关键词： Photodynamic therapy periodontitis toluidine blue O endogenous photosensitizer survival rate

ICAM-1 depletion in the center of immunological synapses is important for calcium releasing in T-cells

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Journal of Innovative Optical Health Sciences 2018年第2期11卷 17-30页

作者： Yuanzhen Suo Wei Lin Yuting Deng zhichao fan Lizeng Qin Guosheng Jiang Yiwei Chu Xunbin Wei Med-X Research Institute and School of Biomedical Engineering Shanghai Jiao Tong University Shanghai 200030P.R.China Department of Immunology and Key Laboratory of Medical Molecular Virology of MOE/MOH School of Basic Medical Sciences and Biotherapy Research Centre Fudan UniversityShanghai 200032P.R.China Shandong Academy of Medical Sciences Jinan 250030P.R.China

T-cell activation requires the formation of the immunological sy napse(IS)bet ween a T-cll and anantigen-presenting cell(AP C)to control the development of the adaptive immune ***-ever,calcium release,an initial signal of T-cell activation,has been found to occur before IS *** mechanism for triggering the calcium signaling and relationship bet ween calciumrelease and IS format ion remains ***,using live-cell imaging,we found that int ercellularadhesion molecule 1(ICAM-1),an essential mdlecule for IS formation,accumulated and then wasdepleted at the center of the synapse before complete IS *** the proces of ICAM1depletion,calcium was *** ICAM-1 failed to be depleted from the center of the synapse,thesustained calcium signaling could not be ***,depletion of ICAM-1 in ISs preferen-tially ccurred with the contact of antigen-specific T-cels and dendritic clls(DCs).Blocking thebinding ofICA M-1 and lymphocy te finction-associated antigen 1(LFA-1),ICAM-1 failed to depleteat the center of the synapse,and calcium release in T-clls *** studying the mechanism ofhow the depletion ofiCA M1 could influence calcium release in T-clls,we found that the movementof ICAM-1 was associat ed with the localization of LFA-1 in the IS,which afected the localization ofcalcium microdomains,ORAIl and mitochondria in ***,the depletion of ICAM-1 in the center of the synapse is an important factor for an initial sust ained calcium release in T-cells.

关键词： T-cell activation immunological synapse ICAM-1 calcium signaling

2-APQC,a small-molecule activator of Sirtuin-3(SIRT3),alleviates myocardial hypertrophy and fibrosis by regulating mitochondrial homeostasis

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Signal Transduction and Targeted Therapy 2024年第6期9卷 2748-2764页

作者： Fu Peng Minru Liao Wenke Jin Wei Liu Zixiang Li zhichao fan Ling Zou Siwei Chen Lingjuan Zhu Qian Zhao Gu Zhan Liang Ouyang Cheng Peng Bo Han Jin Zhang Leilei Fu West China School of Pharmacy and Department of Biotherapy Cancer Center and State Key Laboratory of BiotherapyWest China HospitalSichuan UniversityChengdu 610041China Sichuan Engineering Research Center for Biomimetic Synthesis of Natural Drugs School of Life Science and EngineeringSouthwest Jiaotong UniversityChengdu 610031China State Key Laboratory of Southwestern Chinese Medicine Resources Hospital of Chengdu University of Traditional Chinese MedicineSchool of PharmacyChengdu University of Traditional Chinese MedicineChengdu 611137China School of Pharmaceutical Sciences Health Science CenterShenzhen UniversityShenzhen 518060China School of Traditional Chinese Materia Medica Key Laboratory of Structure-Based Drug Design&Discovery of Ministry of EducationShenyang Pharmaceutical UniversityShenyang 110016China

Sirtuin 3(SIRT3)is well known as a conserved nicotinamide adenine dinucleotide^(+)(NAD^(+))-dependent deacetylase located in the mitochondria that may regulate oxidative stress,catabolism and ATP *** evidence has recently revealed that SIRT3 plays its critical roles in cardiac fibrosis,myocardial fibrosis and even heart failure(HF),through its deacetylation ***,discovery of SIRT3 activators and elucidating their underlying mechanisms of HF should be urgently ***,we identified a new small-molecule activator of SIRT3(named 2-APQC)by the structure-based drug designing strategy.2-APQC was shown to alleviate isoproterenol(ISO)-induced cardiac hypertrophy and myocardial fibrosis in vitro and in vivo rat ***,in SIRT3 knockout mice,2-APQC could not relieve HF,suggesting that 2-APQC is dependent on SIRT3 for its protective ***,2-APQC was found to inhibit the mammalian target of rapamycin(mTOR)-p70 ribosomal protein S6 kinase(p70S6K),c-jun N-terminal kinase(JNK)and transforming growth factor-β(TGF-β)/small mother against decapentaplegic 3(Smad3)pathways to improve ISO-induced cardiac hypertrophy and myocardial *** upon RNA-seq analyses,we demonstrated that SIRT3-pyrroline-5-carboxylate reductase 1(PYCR1)axis was closely assoiated with *** activating PYCR1,2-APQC was shown to enhance mitochondrial proline metabolism,inhibited reactive oxygen species(ROS)-p38 mitogen activated protein kinase(p38MAPK)pathway and thereby protecting against ISO-induced mitochondrialoxidative ***,activation of SIRT3 by 2-APQC could facilitate AMP-activated protein kinase(AMPK)-Parkin axis to inhibit ISO-induced ***,our results demonstrate that 2-APQC is a targeted SIRT3 activator that alleviates myocardial hypertrophy and fibrosis by regulating mitochondrial homeostasis,which may provide a new clue on exploiting a promising drug candidate for the future HF therapeutics.

关键词： SIRT3 homeostasis metabolism