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CCS Chemistry 2024年第3期6卷 719-732页

作者： Lele Sun Fengyun Shen Zijian Xiong Yu Chao chunhai fan Zhuang Liu Institute of Functional Nano&Soft Materials(FUNSOM) Jiangsu Key Lab Carbon Based Functional Materials and DevicesSoochow UniversitySuzhou 215123Jiangsu School of Life Sciences Shanghai UniversityShanghai 200444 School of Chemistry and Chemical Engineering Shanghai Jiao Tong UniversityShanghai 201240

The spatial arrangement of activating ligands is known to have great influence on T cell ***,independently studying each ligand’s spatial organization parameter that affects T cell activation remains a great ***,with DNA origami,we precisely organized the CD3ɛantibodies simulating T cell receptor(TCR)ligands and CD28 antibodies simulating co-stimulatory ligands to interrogate the independent role of TCR-ligand spacing and local copy numbers as well as the spacing between TCR ligands and co-stimulatory ligands on T cell *** found that T cell activation benefited fromlocally concentrated TCR ligands with a shorter spacing and was maximized by an∼38 nm spacing between TCR ligands and co-stimulatory *** T cell expander constructed based on our findings could efficiently expand CD8+T cells for tumor ***,the DNA nanostructurebased ligands’precise arrangement can be a unique tool in studying immune cell activations and cellbased immunotherapies.

关键词： DNA origami T cell activation T cell receptor immune cancer immunotherapy

Hierarchical Self-assembly of Atomically Precise Au Nanoclusters with Molecular Rotor-based Ligands

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Chemical Research in Chinese Universities 2024年第4期40卷 670-674页

作者： WANG Guanzhong LU Hui LI Jiang WANG Lihua ZHU Ying SONG Shiping GE Zhilei LI Qian CHEN Jing fan chunhai Division of Physical Biology Shanghai Institute of Applied PhysicsChinese Academy of SciencesShanghai201800P.R.China University of Chinese Academy of Sciences Beijing100049P.R.China Zhangjiang Laboratory Shanghai201210P.R.China Institute of Materiobiology Department of ChemistryCollege of SciencesShanghai UniversityShanghai200444P.R.China School of Chemistry and Chemical Engineering New Cornerstone Science LaboratoryFrontiers Science Center for Transformative MoleculesZhangjiang Institute for Advanced Study and National Center for Translational MedicineShanghai Jiao Tong UniversityShanghai200240P.R.China

The hierarchical assemblies of precise nanoparticles(NPs)have created materials with emergent properties and ***,the complex assemblies remain unclear at a precise ***,we show the hierarchical self-assembly of atomically precise gold nanoclusters(Au NCs)with molecular rotor-based ligands(MRL),featuring a double-layer *** to two other types of monolayer-protected(MLP)Au NCs,the significantly reduced surface density for MRL Au NCs profoundly influences their assembly behavior within the ***,the long length of rotor-based ligands and the rotational freedom of the phenyl-rings of rotor-based ligands also facilitate the assembly of *** works elucidate the hierarchical assembly on a precise scale,suggesting that the rotor-based ligand’s strategy offers promising potential for designing well-defined and more complex structures in supercrystals.

关键词： Gold nanocluster Molecular rotor Hierarchical assembly Double-layer surface

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运动转化医学:从分子生物力学管窥主动健康

体育科学 2024年第1期44卷 3-11,20页

作者：樊春海程蜀琳上海交通大学转化医学研究院上海201100 上海交通大学化学化工学院上海201100 上海交通大学体育系上海201100

运动在促进健康和预防疾病中一直发挥着积极的作用,大量研究已从多个维度提供了运动有益健康的科学证据,然而人们对其分子和细胞机制却知之甚少。不断深化运动的分子和细胞机制研究,破译特定类型的运动与人体特定组织之间的联系,并将相... 详细信息

运动在促进健康和预防疾病中一直发挥着积极的作用,大量研究已从多个维度提供了运动有益健康的科学证据,然而人们对其分子和细胞机制却知之甚少。不断深化运动的分子和细胞机制研究,破译特定类型的运动与人体特定组织之间的联系,并将相关知识转化到医学和健康实践中来,仍是目前生命科学面临的巨大挑战。在这方面,新兴的系统生物学从信号网络系统层面提供了新的视角,为建立个性化的生活方式和干预方案奠定了基础。而一些全新的分子生物学概念及生物技术(如力敏感蛋白PIEZOs、自噬和微生物组学等)正在全面改变人类对运动(力信号刺激)影响人体系统的认知。研究分析了当前运动研究领域所面临的机遇与挑战,建议从生物力学以及运动科学跨维度交互作用的视角重新认知运动,研究健康敏感指标与运动即刻响应和适应性的相互关系与可信度,促进运动转化医学的发展。

关键词：运动转化医学主动健康生物力学机械刺激自噬多组学中西医结合

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通过单分子荧光显微成像解析活细胞内的生物分子动态

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Science Bulletin 2024年第12期69卷 1823-1828页

作者：高兆帅李茜樊春海侯尚国 School of Chemistry and Chemical Engineering New Cornerstone Science LaboratoryFrontiers Science Center for Transformative MoleculesNational Center for Translational MedicineShanghai Jiao Tong UniversityShanghai 200240China Institute of Systems and Physical Biology Shenzhen Bay LaboratoryShenzhen 518055China

The live-cell biomolecular dynamics refers to the rapid movement,interacting with their surroundings,and the transformation of biomolecules within living *** understanding of biomolecular dynamics in their native state may reveal fundamental molecular mechanisms of cell function and disease formation[1].Also,understanding the disease associated biomolecular dynamics can assist in disease diagnosis and therapy.

关键词：单分子荧光生物分子显微成像

Special topic for “single-molecule, single-particle and single-cell bioimaging”

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Science China Chemistry 2017年第10期60卷 1265-1266页

作者： chunhai fan Xiaohong fang Shanghai Institute of Applied Physics Chinese Academy of Sciences Institute of Chemistry Chinese Academy of Sciences

Revealing the behavior of single molecules in single live cells provides a fundamental approach to understand cellular organization and *** the rapid merging of biotechnology and nanotechnology in recent years,single plasmonic nanoparticle sensors have endowed a new dimension to the imaging scale given their comparable size to biomolecules such as nucleic acids or antibodies.

关键词： nanotechnology endowed biotechnology merging comparable technological nucleic overview contributions advances

A Chemical Approach for Real-time Monitoring Neuronal Activities

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Chemical Research in Chinese Universities 2020年第4期36卷 729-730页

作者： LI Qian fan chunhai School of Chemistry and Chemical Engineering Shanghai Jiao Tong UniversityShanghai 200240P.R.China

In the latest issue of Nature Nanotechnology,Ling and coworkers reported the first potassium nanosensor applicable in a freely moving *** was developed by embedding the mesoporous silica nanoparticle framework with optical potassium indicators and then shielding the framework with an ultrathin layer of a potassium-permeable *** nanosensor achieved highly sensitive monitoring of the dynamic changes of the extracellular potassium in the brain of freely moving mice with epilepsy.

关键词： potassium epilepsy moving

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基于核酸框架的分子机器

中国科学：化学 2024年第5期54卷 802-810页

作者：依沙克江·赛麦提李敏闫庆龙左小磊樊春海中国科学院上海应用物理研究所物理生物学研究室界面物理与技术重点实验室上海201800 中国科学院大学北京100049 上海交通大学医学院分子医学研究院上海市核酸化学与纳米医学重点实验室上海200127 祥符实验室嘉善314102 上海交通大学化学化工学院转化医学研究院上海200240

分子机器是一种由分子构建的微型设备,在受到适当的刺激如光、温度、pH或电磁场时,它能够在分子水平上执行类似宏观机器的机械运动.然而,分子机器的研究仍面临着许多技术挑战,包括如何精确控制分子机器的运动,如何构建大规模的分子机器... 详细信息

分子机器是一种由分子构建的微型设备,在受到适当的刺激如光、温度、pH或电磁场时,它能够在分子水平上执行类似宏观机器的机械运动.然而,分子机器的研究仍面临着许多技术挑战,包括如何精确控制分子机器的运动,如何构建大规模的分子机器系统等.作为有潜力的分子自组装技术,利用DNA纳米技术可以构建复杂的刺激响应纳米机器并精确调控其在分子水平的运动.本文中,我们简单介绍了DNA纳米技术的组装原理,综述了响应DNA链置换、光、热、pH和电场等不同类型刺激的核酸框架分子机器,并探讨了它们在药物递送、构建三维等离子体光学器件以及作为生物分子标尺等方面的应用.

关键词：分子机器核酸框架 DNA链置换药物递送等离子体光学器件

Biomacromolecular nanostructures-based interfacial engineering: from precise assembly to precision biosensing

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National Science Review 2018年第5期5卷 740-755页

作者： fan Yang Xiaolei Zuo chunhai fan Xian-En Zhang Division of Physical Biology & Bioimaging Center Shanghai Synchrotron Radiation FacilityShanghai Institute of Applied PhysicsChinese Academy of Sciences National Key Laboratory of Biomacromolecules CAS Excellence Center for BiomacromoleculesInstitute of BiophysicsChinese Academy of Sciences School of Laboratory Medicine Hubei University of Chinese Medicine

Biosensors are a type of important biodevice that integrate biological recognition elements, such as enzyme,antibody and DNA, and physical or chemical transducers, which have revolutionized clinical diagnosis especially under the context of point-of-care tests. Since the performance of a biosensor depends largely on the bio–solid interface, design and engineering of the interface play a pivotal role in developing quality biosensors. Along this line, a number of strategies have been developed to improve the homogeneity of the interface or the precision in regulating the interactions between biomolecules and the interface. Especially,intense efforts have been devoted to controlling the surface chemistry, orientation of immobilization,molecular conformation and packing density of surface-confined biomolecular probes(proteins and nucleic acids). By finely tuning these surface properties, through either gene manipulation or self-assembly, one may reduce the heterogeneity of self-assembled monolayers, increase the accessibility of target molecules and decrease the binding energy barrier to realize high sensitivity and specificity. In this review, we summarize recent progress in interfacial engineering of biosensors with particular focus on the use of protein and DNA nanostructures. These biomacromolecular nanostructures with atomistic precision lead to highly regulated interfacial assemblies at the nanoscale. We further describe the potential use of the high-performance biosensors for precision diagnostics.

关键词： biosensor interface engineering homogeneity orientation control fused proteins DNA nanostructures

Framework Nucleic Acids for Cell Imaging and Therapy

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Chemical Research in Chinese Universities 2020年第1期36卷 1-9页

作者： GE Zhilei LI Qian fan chunhai School of Chemistry and Chemical Engineering and Institute of Molecular MedicineRenji HospitalSchool of MedicineShanghai Jiao Tong UniversityShanghai 200240P.R.China

Over the past decade,structural DNA nanotechnology has been well developed to be a promising and powerful technique to generate various nanostructures with programmability,spatial organization and *** the advent of computer-aided tools,framework nucleic acids have been employed in a series of biomedical applications,ranging from biosensing,bioimaging,diagnosis,to *** this review,we summarized recent advances in the construction of precisely assembled DNA nanostructures,and DNA-engineered *** also outlined the challenges and opportunities for the translational applications of framework nucleic acids.

关键词： DNA nanotechnology Framework nucleic acid DNA origami Therapy Cellular imaging

DNA framework-engineered electrochemical biosensors

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Science China(Life Sciences) 2020年第8期63卷 1130-1141页

作者： fan Li Qian Li Xiaolei Zuo chunhai fan Institute of Molecular Medicine Renji HospitalSchools of MedicineShanghai Jiao Tong UniversityShanghai 200127China School of Chemistry and Chemical Engineering Shanghai Jiao Tong UniversityShanghai 200240China

Self-assembled DNA nanostructures have shown remarkable potential in the engineering of biosensing interfaces,which can improve the performance of various *** particular,by exploiting the structural rigidity and programmability of the framework nucleic acids with high precision,molecular recognition on the electrochemical biosensing interface has been significantly enhanced,leading to the development of highly sensitive and specific biosensors for nucleic acids,small molecules,proteins,and *** this review,we summarize recent advances in DNA framework-engineered biosensing interfaces and the application of corresponding electrochemical biosensors.

关键词： framework nucleic acids biosensing interface electrochemical biosensors biomarkers DNA