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Journal of Magnesium and Alloys 2024年第1期12卷 394-404页

作者： Lokesh Choudhary Parama Chakraborty Banerjee R.K.Singh Raman Derrek E.Lobo Christopher D.Easton Mainak Majumder frank Witte Jörg f.Löffler Department of Mechanical and Aerospace Engineering Monash UniversityClayton CampusMelbourneVIC 3800Australia Department of Chemical and Biological Engineering Monash UniversityClayton CampusMelbourneVIC 3800Australia Nanoscale Science and Engineering Laboratory(NSEL) Bldg 37Monash UniversityClayton CampusMelbourneVIC 3800Australia CSIRO Materials Science and Engineering Bayview AvenueClaytonVIC 3168Australia Julius Wolff Institute and Berlin Brandenburg Center for Regenerative Therapies Charité–Universitätsmedizin BerlinAugustenburger Platz 113353 BerlinGermany Laboratory of Metal Physics and Technology Department of MaterialsETH Zurich8093 ZurichSwitzerland

Biodegradable implants are critical for regenerative orthopaedic procedures,but they may suffer from too fast corrosion in human-body *** necessitates the synthesis of a suitable coating that may improve the corrosion resistance of these implants without compromising their mechanical *** this study,an AZ91 magnesium alloy,as a representative for a biodegradable Mg implant material,was modified with a thin reduced graphene oxide(RGO)-calcium carbonate(CaCO_(3))composite *** analytical and in-vitro electrochemical characterization reveals that this coating significantly improves the corrosion resistance and mechanical integrity,and thus has the potential to greatly extend the related application field.

关键词： Graphene coating Biodegradable implant Hydroxyapatite Corrosion Magnesium alloy

Pronuclear formation by ICSI using chemically activated ovine oocytes and zona pellucida bound sperm

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Journal of Animal Science and Biotechnology 2017年第2期8卷 370-377页

作者： J.E.Hernández-Pichardo Y.Ducolomb S.Romo M.E.Kjelland R.fierro f.Casillas M.Betancourt División de Ciencias Biológicas y de la Salud Departamento de Producción Agrícola y AnimalUniversidad Autónoma Metropolitana-XochimilcoCiudad de MexicoMexico Division de Ciencias Biologicas y de la Salud Departamento de Ciencias de la SaludUniversidad Autónoma Metropolitana-IztapalapaSan Rafael Atlixco 186CP 09340 Ciudad de MexicoMexico Departamento de Ciencias Pecuarias Facultad de EstudiosSuperiores CuautitlanUNAMCiudad de MexicoEstado de MexicoMexico Conservation Genetics&BiotechLLCValley CityNDUSA Division de Ciencias Biologicas y de la Salud Departamento de Biologia de la ReproduccionUniversidad Autonoma Metropolitana-lztapalapaCiudad de MexicoMexico Doctorado en Biologia Experimental Universidad Autonoma Metropolitana-lztapalapaCiudad de MexicoMexico

Background： In order to improve ICSI, appropiate sperm selection and oocyte activation is necessary. The objective of the present study was to determine the efficiency of fertilization using ICSI with chemically activated ovine oocytes and sperm selected by swim up（SU） or swim up ＋ zona pellucida（SU ＋ ZP） ***： Experiment 1, 4–20 replicates with total 821 in vitro matured oocytes were chemically activated with ethanol, calcium ionophore or ionomycin, to determine oocyte activation（precense of one PN）. Treatments showed similar results（54, 47, 42 %, respectively） but statistically differents（P 0.05）.Conclusions： Chemical activation induces higher ovine oocyte activation than mechanical activation. Ethanol slightly displays higher oocyte activation than calcium ionophore and ionomicine. Sperm selection with SU ＋ ZP increased AR/A and AR/D rates in comparison with SU in fresh and frozen-thawed sperm. According to this, in terms of fertilization rates, chemical activation after ICSI increased oocyte PN formation compared to mechanical activation. Also, fresh sperm treated with SU and SU ＋ ZP were significantly different than frozen-thawed sperm,but between sperm treatments no significant differences were obtained.

关键词： Ethanol ICSI Oocyte activation Ovine Pronucleus Zona pellucida

Ultrastructural effect of sildenafil citrate on corpus cavernosum and other genital organs in female rats

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Asian Journal of Andrology 2003年第1期5卷 37-41页

作者： K.Kilinc M.I.Gündüz B.H.Gümüs S.Vatansever f.Kaymaz Department of Urology University of Celal Bayar 45010 Manisa TurkeyDepartment of Urology University of Celal Bayar 45010 Manisa TurkeyDepartment of Urology University of Celal Bayar 45010 Manisa TurkeyDepartment of Histology University of Celal Bayar 45010 Manisa TurkeyDepartment of Histology University of Hacettepe Turkey

Aim: To determine the ultrastructural effects of sildenafil on the female genital organs. Methods: Twenty female cycling Wistar albino rats weighing 250±20 g were randomly divided into two groups of 10 each. Rats of one group were gavaged with 0.5 mg·kg-1·d-1 of sildenafil 3 days in a week for 4 weeks and the other served as the controls. After cessation of treatment animals were sacrificed by cervical dislocation under methoxyflurane anaesthesia. The clitoris, vagina, uterus and bartholin glands were taken at the estrous and were fixed with 10 % formalin solution for light microscopy and 2.5 % glutaraldehyde and osmic acid for electron microscopy. Results: Under the light microscope, the fibrocollageous tissue was found increased, the capillaries enlarged and the connecting tissue elements increased in the corpus cavernosum in the treated group. On electron microscopy, increased connective tissue, fibroblasts with notched nucleus, shorten immature collagen fibers without striation were seen. Abundant foldings and penetration with collagen bundles were observed in the basal membrane. Large connection complexes, especially gap junctions among the wide capillary endothelial cells were observed. Conclusion: There are evident histological changes due to sildenafil citrate in female rat corpus cavernosum. The clitoris and bartholin glands were the most effected organs. While the histopathological changes of clitoral tissue could be expected, an increase in the mass of bartholin gland was surprised.

关键词： sildenafil female clitoris histopathologic effects

NUMERICAL SOLUTION Of DIffERENTIAL EQUATIONS IN IRREGULAR PLANE REGIONS USING QUALITY STRUCTURED CONVEX GRIDS

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International Journal of Modeling, Simulation, and Scientific Computing 2013年第2期4卷 115-129页

作者： f.DOM´INGUEZ-MOTA P.fERNANDEZ-VALDEZ S.MENDOZA-ARMENTA G.TINOCO-GUERRERO J.G.TINOCO-RUIZ facultad de Ciencias f´ısico Matem´aticas Universidad Michoacana de San Nicol´as de Hidalgo Edificio“B” Ciudad Universitaria C.P.58040MoreliaMexico

The variational grid generation method is a powerful tool for generating structured convex grids on irregular simply connected domains whose boundary is a polygonal Jordan *** examples that show the accuracy of a finite difference approximation to the solution of a Poisson equation using this kind of structured grids have been recently *** this paper,we compare the accuracy of the numerical solution calculated using those structured grids and finite differences against the solution obtained with Delaunay-like triangulations on irregular regions.

关键词： Numerical grid generation direct generation method finite difference method finite element method Delaunay triangulation.

Chemical dissolution resistance of anodic oxide layers formed on aluminum

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Transactions of Nonferrous Metals Society of China 2011年第7期21卷 1673-1679页

作者： W.BENSALAH M.fEKI M.WERY H.f.AYEDI Unité de recherche de Chimie Industrielle et Matériaux (URCIM) ENIS.B.P.W.SfaxTunisie IUT Mesures Physiques d'Orsay-Université Paris XI Plateau du Moulon91400 ORSAYFrance

Chemically resistant anodic oxide layers were formed on pure aluminum substrates in oxalic acid-sulphuric acid *** dissolution tests of the obtained anodic layers were achieved in accordance with the ASTM B 680-80 specifications：35mL/L 85% H3PO4＋20g/L CrO3 at 38℃.Influence of oxalic acid concentration,bath temperature and anodic current density on dissolution rate and coating ratio was examined,when the sulphuric acid concentration was maintained at 160g/*** was found that chemically resistant and compact oxide layers were produced under low operational temperature （5℃） and high current densities （3A/dm^2）.A beneficial effect was observed concerning the addition of oxalic acid （18g/L）.The morphology and the composition of the anodic oxide layer were examined by scanning electron microscopy （SEM）,atomic force microscopy （AfM） and glow-discharge optical emission spectroscopy （GDOES）.

关键词： aluminum anodic oxide layer oxalic acid-sulphuric anodization dissolution rate coating ratio

Upregulation of the APE1 and H2AX genes and miRNAs involved in DNA damage response and repair in gastric cancer

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Genes & Diseases 2019年第2期6卷 176-184页

作者： fernanda S.Manoel-Caetano Ana flávia T.Rossi Gabriela Calvet de Morais fábio Eduardo Severino Ana Elizabete Silva Department of Biology UNESPSāo Paulo State UniversityCampus of Sāo Josédo Rio PretoRua Cristòvāo Colombo226515.054-000Sāo Josédo Rio PretoSāo PauloBrazil Department of Surgery and Orthopedics Faculty of MedicineUNESPSāo Paulo State UniversityCampus of BotucatuAv.Prof.Mário Rubens Guimarāes Montenegros/n18.618-687BotucatuSāo PauloBrazil

Gastric cancer remains one of the leading causes of cancer-related death worldwide,and most of the cases are associated with Helicobacter pylori *** bacterium promotes the production of reactive oxygen species(ROS),which cause DNA damage in gastric epithelial *** this study,we evaluated the expression of important genes involved in the recognition of DNA damage(ATM,ATR,and H2AX)and ROS-induced damage repair(APE1)and the expression of some miRNAs(miR-15a,miR-21,miR-24,miR-421 and miR-605)that target genes involved in the DNA damage response(DDR)in 31 fresh tissues of gastric *** v3.1.1 was used to construct the postulated miRNA:mRNA interaction *** performed by real-time quantitative PCR exhibited significantly increased levels of the APE1(RQ=2.55,pficantly elevated levels of miR-21,miR-24 and miR-421 were observed in diffuse-type gastric *** analysis reinforced some of the gene:gene(ATM/ATR/H2AX)and miRNA:mRNA relationships obtained also with the interaction ***,our findings show that tumor cells from gastric cancer presents deregulation of genes and miRNAs that participate in the recognition and repair of DNA damage,which could confer an advantage to cell survival and proliferation in the tumor microenvironment.

关键词： DNA damage response DNA repair Gastric cancer Gene expression microRNA

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Biosurfactant-assisted phytoremediation of potentially toxic elements in soil:Green technology for meeting the United Nations Sustainable Development Goals

作者： black.f

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Pedosphere 2022年第1期32卷 198-210页

作者： Songita SONOWAL Amy R.NAVA Sanket J.JOSHI Siddhartha Narayan BORAH Nazim f.ISLAM Soumya PANDIT Ram PRASAD Hemen SARMA Department of Botany School of Life SciencesMahatma Gandhi Central UniversityMotihari 845401India Department of Molecular&Cellular Physiology Stanford UniversityStanfordCA 94305SA Oil&Gas Research Center Central Analytical and Applied Research UnitSultan Qaboos UniversityMuscat 123Oman Centre for the Environment Indian Institute of Technology GuwahatiGuwahati 781039India Institutional Biotech Hub(IBT Hub) Department of BotanyNanda Nath Saikia CollegeTitabar 785630India Department of Life Sciences School of Basic Sciences and ResearchSharda UniversityGreater Noida 201306India

Biosurfactants are biomolecules produced by microorganisms, low in toxicity, biodegradable, and relatively easy to synthesize using renewable waste substrates. Biosurfactants are of great importance with a wide and versatile range of applications, including the bioremediation of contaminated sites. Plants may accumulate soil potentially toxic elements(PTEs), and the accumulation efficacy may be further enhanced by the biosurfactants produced by rhizospheric microorganisms. Occasionally, the growth of bacteria slows down in adverse conditions, such as highly contaminated soils with PTEs. In this context,the plant's phytoextraction capacity could be improved by the addition of metal-tolerant bacteria that produce biosurfactants. Several sources, categories,and bioavailability of PTEs in soil are reported in this article, with the focus on the cost-effective and sustainable soil remediation technologies, where biosurfactants are used as a remediation method. How rhizobacterial biosurfactants can improve PTE recovery capabilities of plants is discussed, and the molecular mechanisms in bacterial genomes that support the production of important biosurfactants are listed. The status and cost of commercial biosurfactant production in the international market are also presented.

关键词： bacterial genome metal-tolerant bacteria phytoextraction technology plant growth-promoting rhizobacteria rhizospheric microorganism

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