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Diagnostic value of AFP-L3 and PIVKA-Ⅱin hepatocellular carcinoma according to total-AFP

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World Journal of Gastroenterology 2013年第3期19卷 339-346页

作者： Jong Young Choi Seung Won Jung Hee Yeon Kim Myungshin Kim Yonggoo Kim Dong Goo Kim eun-jee oh Department of Internal Medicine School of MedicineThe Catholic University of KoreaSeoul 137-701South Korea Department of Laboratory Medicine and Laboratory Development Evaluation Center School of MedicineThe Catholic University of KoreaSeoul 137-701South Korea Department of Surgery School of MedicineThe Catholic University of KoreaSeoul 137-701South Korea

AIM:To evaluate diagnostic value ofα-fetoprotein (AFP)-L3 and prothrombin induced by vitamin K absence-Ⅱ(PIVKA-Ⅱ)in hepatocellular carcinoma(HCC). METHODS:One hundred and sixty-eight patients during routine HCC surveillance were included in this *** the 168 patients,90(53.6%)had HCC including newly developed HCC(n=82)or recurrent HCC after treatment(n=8).Sera were obtained during their first evaluation for HCC development and at the time of HCC diagnosis before commencing HCC *** was diagnosed by histological examination,appropriate imaging characteristics-computed tomography or magnetic resonance *** sera were collected from 78 patients with benign liver disease(BLD),which were obtained during routine surveillance with a suspicion of ***,AFP-L3 and PIVKA-Ⅱwere measured in the same serum by microchip capillary electrophoresis and liquid-phase binding assay on a micro-total analysis system Wako i30 auto *** performance characteristics of three tests and combined tests for the diagnosis of HCC were obtained using receiver operating characteristic curves in all populations and subgroups with AFP200 ng/*** the 78 BLD patients,74(94.9%)patients had AFP5%) and PIVKA-Ⅱ(cut-off>40 AU/L),the sensitivity were 94.4%and specificity were 75.6%in all *** 112 patients

关键词： α-fetoprotein Prothrombin induced by vitamin K absence-Ⅱ Hepatocellular carcinoma Diagnosis Tumor marker

SeSaMe PS Function:Functional Analysis of the Whole Metagenome Sequencing Data of the Arbuscular Mycorrhizal Fungi

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Genomics, Proteomics & Bioinformatics 2020年第5期18卷 613-623页

作者： jee eun Kang Antonio Ciampi Mohamed Hijri Institut de Recherche en Biologie Vegetale Departement de Sciences BiologiquesUniversite de MontrealQC H1X 2B2Canada Department of Epidemiology Biostatistics and Occupational HealthMcGill UniversityMontrealQC H3A 1A2Canada Creative Research Initiatives Center for Molecular Biology of Translation School of Life SciencesKorea UniversitySeoul 02841Republic of Korea African Genome Center AgroBioSciencesMohammed Ⅳ Polytechnic University(UM6P)43150 Ben GuerirMorocco

In this study,we introduce a novel bioinformatics program,Spore-associated Symbiotic Microbes Position-specific Function(SeSaMe PS Function),for position-specific functional analysis of short sequences derived from metagenome sequencing data of the arbuscular mycorrhizal *** unique advantage of the program lies in databases created based on genus-specific sequence properties derived from protein secondary structure,namely amino acid usages,codon usages,and codon contexts of 3-codon DNA *** PS Function searches a query sequence against reference sequence database,identifies 3-codon DNA 9-mers with structural roles,and creates a comparative dataset containing the codon usage biases of the 3-codon DNA 9-mers from 54 bacterial and fungal *** program applies correlation principal component analysis in conjunction with K-means clustering method to the comparative dataset.3-codon DNA 9-mers clustered as a sole member or with only a few members are often structurally and functionally distinctive sites that provide useful insights into important molecular *** program provides a versatile means for studying functions of short sequences from metagenome sequencing and has a wide spectrum of *** PS Function is freely accessible at ***.

关键词： SeSaMe Spore-associated symbiotic microbes Position-specific function Outlier Metagenome

SeSaMe:Metagenome Sequence Classification of Arbuscular Mycorrhizal Fungi-associated Microorganisms

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Genomics, Proteomics & Bioinformatics 2020年第5期18卷 601-612页

作者： jee eun Kang Antonio Ciampi Mohamed Hijri Institut de Recherche en Biologie Vegetale Departement de Sciences BiologiquesUniversite de MontrealMontrealQC H1X 2B2Canada Department of Epidemiology Biostatistics and Occupational HealthMcGill UniversityMontrealQC H3A 1A2Canada Creative Research Initiatives Center for Molecular Biology of Translation School of Life SciencesKorea UniversitySeoul 02841Republic of Korea African Genome Center Agro BioSciencesMohammed Ⅵ Polytechnic University(UM6P)43150 Ben GuerirMorocco

Arbuscular mycorrhizal fungi(AMF)are plant root symbionts that play key roles in plant growth and soil *** are obligate biotrophic fungi that form coenocytic multinucleated hyphae and *** studies have shown that diverse microorganisms live on the surface of and inside their mycelia,resulting in a metagenome when whole-genome sequencing(WGS)data are obtained from sequencing AMF cultivated in *** metagenome contains not only the AMF sequences,but also those from associated *** this study,we introduce a novel bioinformatics program,Spore-associated Symbiotic Microbes(SeSaMe),designed for taxonomic classification of short sequences obtained by next-generation DNA sequencing.A genus-specific usage bias database was created based on amino acid usage and codon usage of a three consecutive codon DNA 9-mer encoding an amino acid trimer in a protein secondary *** program distinguishes between coding sequence(CDS)and non-CDS,and classifies a query sequence into a genus group out of 54 genera used as *** mean percentages of correct predictions of the CDS and the non-CDS test sets at the genus level were 71% and 50% for bacteria,68% and 73% for fungi(excluding AMF),and 49% and 72% for AMF(Rhizophagus irregularis),*** provides not only a means for estimating taxonomic diversity and abundance but also the gene reservoir of the reference taxonomic groups associated with ***,it enables users to study the symbiotic roles of associated *** can also be applicable to other microorganisms as well as soil *** is freely available at ***.

关键词： SeSaMe Spore-associated symbiotic microbes Arbuscular mycorrhizal fungi Taxonomic classification 3-codon DNA 9-mer

Development of 3D bicontinuous metal-intermetallic composites through subsequent alloying process after liquid metal dealloying

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Journal of Magnesium and Alloys 2023年第11期11卷 4274-4281页

作者： jee eun Jang Jihye Seong Soo-Hyun Joo Sung Hyuk Park School of Materials Science and Engineering Kyungpook National UniversityDaegu 41566Republic of Korea Department of Materials Science and Engineering Dankook UniversityCheonan 31116Republic of Korea

This study presents a novel process for the fabrication of metal-intermetallic composites with a 3D bicontinuous structure, achieved through a combination of liquid metal dealloying(LMD) and subsequent alloying. Initially, porous Ti structures are produced using the LMD process, followed by immersion in a molten Mg-3Al(wt%) metal. Due to the higher thermodynamic miscibility of Al with Ti compared to Mg, the concentration of Al in the Ti matrix increases as the immersion time increases. This results in a sequential phase transition within the Ti matrix: α-Ti → Ti_(3)Al → Ti Al. The phase transition considerably affects the hardness and strength of the composite material,with the Mg-Ti_(3)Al-Ti Al composite exhibiting a maximum hardness nearly twice as high as that of the conventional Mg-Ti composite. This innovative process holds potential for the development of various bicontinuous metal-intermetallic composites.

关键词： Liquid metal dealloying Subsequent alloying Metal–intermetallic composite 3D bicontinuous structure Hardness

Dynamic genetic features of eukaryotic plankton diversity in the Nakdong River estuary of Korea

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Chinese Journal of Oceanology and Limnology 2017年第4期35卷 844-857页

作者： LEE jee eun CHUNG Ik Kyo LEE Sang-Rae Division of Earth Environmental System Oceanography Major Pusan National University Marine Research Institute Pusan National University

Estuaries are environments where freshwater and seawater mix and they display various salinity *** construction of river barrages and dams has rapidly changed these environments and has had a wide range of impacts on plankton *** understand the dynamics of such communities,researchers need accurate and rapid techniques for detecting plankton *** evaluated the diversity of eukaryotic plankton over a salinity gradient by applying a metagenomics tool at the Nakdong River estuary in *** samples were collected on three dates during summer and autumn of 2011 at the Eulsukdo Bridge at the mouth of that *** the 18 S rDNA allowed us to analyze 456 clones and 122 *** sequences revealed various taxonomic groups and cryptic genetic variations at the intra-and inter-specific *** analyzing the same station at each sampling date,we observed that the phylotypes presented a salinity-related pattern of diversity in *** variety of species within freshwater samples reflected the rapid environmental changes caused by freshwater *** phylotypes accounted for the highest proportion of overall diversity in the seawater *** diatoms and dinoflagellates were observed in the freshwater,brackish and seawater *** biological data for species composition demonstrate the transitional state between freshwater and ***,this metagenomics information can serve as a biological indicator for tracking changes in aquatic environments.

关键词： 18S rDNA clone library metagenome estuary salinity gradient

Evaluation of the safety of hair essence containing 0.05% purified bee venom on the skin and eyes of rabbits

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Journal of Traditional Chinese Medicine 2020年第1期40卷 67-72页

作者： Mi Kyung Lim eun Hye Han Sang Ho Lee jee Hee Kim R&D Center KoreaeundanGyeonggi-do 13207Republic of Korea

OBJECTIVE:To investigate the safety of hair essence containing 0.05%purified bee venom(HE-PBV)on the skin and eyes of New Zealand White ***:HE-PBV which contained 0.05%PBV,purified water,and glycerin,was used as the test *** skin-irritation test(SIT)and eye-irritation test(EIT)were conducted according to the Draize *** the SIT,HE-PBV(0.5 m L)dropped gauze was attached both intact and abraded skin for 24 *** other side of the skin was used as *** 24 and 72 h,the treatment site was observed and scored according to evaluation criteria for skin *** the EIT,the rabbits were divided into two groups:eye-washed(three rabbits)and non-eye-washed(six rabbits).HE-PBV(0.1 m L)was squirted into the right eye of *** left eye was untreated and used as a ***,20-30-s later,the eyes of rabbits in the eye-washed group were washed with^50 m L of physiologic(0.9%)salt ***,1,2,3,4 and 7 d after the start of the EIT,the eyes and behavior of the rabbits were *** degree of eye irritation elicited by HE-PBV was determined in three steps and then the criteria of the classification of eye-irritation ***:The SIT revealed erythema and edema at the site of HE-PBV *** 72 h,the body weight of rabbits was reduced slightly,but other symptoms(except erythema and edema)were not *** Primary Irritation Index score was0.6,and HE-PBV was deemed to be a slight *** EIT did not show mortality or body-weight fluctuation,but hyperemic conjunctiva and eyelid closure were noted after HE-PBV *** for these results,the score for the ophthalmic response on days 1,2,3,4 and 7 was 0,and HE-PBV was deemed to be a ***:These data suggest that HE-PBV did not elicit eye irritation,but was a slight irritant to the skin of rabbits;the latter slight would have been due to the excipients used in manufacture of the hair essence because PBV has been shown to be safe.

关键词： Alopecia Bee Venoms Hair essence Skin irritancy tests Eye irritancy tests Complementary therapies

Effects of ocean acidifi cation driven by elevated CO_2 on larval shell growth and abnormal rates of the venerid clam, Mactra veneriformis

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Chinese Journal of Oceanology and Limnology 2016年第6期34卷 1191-1198页

作者： jee-Hoon KIM Ok Hwan YU eun Jin YANG Sung-Ho KANG Won KIM eun Jung CHOY Division of Polar Ocean Environment Research Korea Polar Research Institute Korea Institute of Ocean Science and Technology School of Biological Sciences Seoul National University Marine Ecosystem Research Division Korea Institute of Ocean Science and Technology

A bstract The venerid clam(M actra veneriformis Reeve 1854) is one of the main cultured bivalve species in intertidal and shallow subtidal ecosystems along the west coast of Korea. To understand the effects of ocean acidifi cation on the early life stages of Korean clams, we investigated shell growth and abnormality rates and types in the D-shaped, umbonate veliger, and pediveliger stages of the venerid clam M. veneriformis during exposure to elevated seawater pCO_2. In particular, we examined abnormal types of larval shell morphology categorized as shell deformations, shell distortions, and shell fi ssures. Specimens were incubated in seawater equilibrated with bubbled CO_2-enriched air at(400±25)×10^(-6)(ambient control),(800±25)×10^(-6)(high pCO_2), or(1 200±28)×10^(-6)(extremely high pCO_2), the atmospheric CO_2 concentrations predicted for the years 2014, 2084, and 2154(70-year intervals; two human generations), respectively, in the Representative Concentration Pathway(RCP) 8.5 scenario. The mean shell lengths of larvae were signifi cantly decreased in the high and extremely high pCO_2 groups compared with the ambient control groups. Furthermore, under high and extremely high pCO_2 conditions, the cultures exhibited signifi cantly increased abundances of abnormal larvae and increased severity of abnormalities compared with the ambient control. In the umbonate veliger stage of the experimental larvae, the most common abnormalities were shell deformations, distortions, and fi ssures; on the other hand, convex hinges and mantle protuberances were absent. These results suggest that elevated CO_2 exerts an additional burden on the health of M. veneriformis larvae by impairing early development.

关键词： abnormality larval shell growth Mactra veneriformis ocean acidifi cation pCO2

Structure-activity relationship of lysophosphatidylcholines in HL-60 human leukemia cells

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Acta Pharmacologica Sinica 2004年第11期 137-140页

作者： eun-hee LEE,jee H JUNG,Dong-soon IM Laboratories of Pharmacology and Marine Natural Product Laboratories of Pharmacology and Marine Natural Product Laboratories of Pharmacology and Marine Natural Product College of Pharmacy Pusan National University Busan 609-735 Korea College of Pharmacy Pusan National University Busan 609-735 Korea College of Pharmacy Pusan National University Busan 609-735 Korea

AIM: To explore the structure-activity relationship of lysophosphatidylcholine (LPC) and lysolipid molecules from a marine sponge and ladybirds. METHODS: We tested three synthetic LPCs and four natural lysolipids on Ca2+ mobilization in HL-60 human leukemia cells. RESULTS: We observed lysolipid-mediated Ca2+ mobilization. The activity was the same in both ester- and ether-linked lysolipids, and introduction of a double bond or methoxy group on the alkyl chain did not significantly modulate the activity. However, replacement of trimethylammonium moiety in the choline structure with ammonium moiety reduced the activity. Furthermore, change of the alkyl chain length influenced the Ca2+ response. CONCLUSION: LPC-induced Ca2+ mobilization might be dependent on the length of alkyl chain and the presence of choline moiety in HL-60 leukemia cells.

关键词： lysophosphatidylcholines leukemia calcium signaling structure-activity relationship platelet activating factor

Gene editing particle system as a therapeutic approach for drug-resistant colorectal cancer

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Nano Research 2020年第6期13卷 1576-1585页

作者： jee-Yeon Ryu You Jung Choi eun-Jeong Won Emmanuel Hui Ho-Shik Kim Young-Seok Cho Tae-Jong Yoon Laboratory of Nanopharmacy College of PharmacyResearch Institute of Pharmaceutical Science and Technology(RIPST)Ajou UniversitySuwon16499Republic of Korea Division of Gastroenterology Department of Internal MedicineSeoul St.Mary’s HospitalCollege of MedicineThe Catholic University of KoreaSeoul06591Republic of Korea Moogene Medi Co. Ltd.Seongnam13488Republic of Korea

The epidermal growth factor receptor(EGFR)pathway plays an important role in the progression of colorectal cancer(CRC).Anti-EGFR drugs based on antibodies have been widely used for treating CRC through inducing the cell death ***,the majority of CRC patients will inevitably develop drug-resistance during anti-EGFR drug treatment,which is mainly caused by a point mutation in the KRAS *** developed a nanoliposomal(NL)particle containing the Cas9 protein and a single-guide RNA(sgRNA)complex(Cas9-RNP),for genomic editing of the KRAS *** NL particle is composed of bio-compatible lipid compounds that can effectively encapsulate *** modifying the NL particle to include the appropriate antibody,it can specifically recognize EGFR expressing CRC and effectively deliver the gene-editing *** conditions of NL treatment were optimized using a KRAS mutated CRC in vivo mouse *** with KRAS-mutated CRC showed drug resistance against cetuximab,a therapeutic antibody *** treating the mice with the KRAS gene-editing NL particles,the implanted tumors showed a dramatic decrease in *** results demonstrated that this genomic editing complex has great potential as a therapeutic carrier system for the treatment of drug-resistant cancer caused by a point mutation.

关键词： nanoliposome clustered regularly interspaced short palindromic repeat and associated Cas9 nuclease(CRISPR/Cas9) KRAS mutation drug-resistance colorectal cancer