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Impact of Feedlot on Soil Phosphorus Concentration

Journal of Environmental Protection 2011年第3期2卷 280-286页

作者： Nicolás Wyngaard Liliana Picone Cecilia Videla ester zamuner Néstor Maceira 不详

Raising cattle in feedlots is becoming more common in Argentina, but there is little information available about the effects of this practice on soil phosphorus (P) dynamics. In this study, concentrations of water and Bray -1 extractable soil P were quantified in a feedlot (upper and lower slope positions in the feedlot pen), runoff area and pasture during 2006 - 2008. The feedlot showed higher P concentrations in all P forms, soluble reactive P, soluble nonreactive, total soluble P, and Bray-P1 when compared to runoff area and pasture. Most of the total soluble P was soluble reactive P from the soil in the feedlot and the runoff area, while soluble nonreactive P predominated in the pasture. Concentrations of total soluble P were elevated in the feedlot at the soil surface, ranging on average from 158.71 to 245.86 mg?P?kg?1, and had a rapid decrease within the first 20 cm and remained relatively low, about 25.53 - 27.33 mg?P?kg?1. The evidence that total soluble P concentration in the feedlot was significantly (p < 0.05) higher than that registered in the pasture at 20 - 40 and 40 - 60 cm soil depth suggests a potential transfer of P through the soil from the surface. Although the feedlot had a moderate increase of 23.05 to 24.55 mg?P?kg?1 from the background concentration, it may represent a long-term source of increased nutrient loading to groundwater. Soil extractable Bray-1 P in the top 0 - 2.5 cm fluctuated from 659.26 to 45.36 mg?P?kg?1 in the feedlot and pasture, respectively. The relationship between soil P extracted by test and TSP was linear, and correlation coefficient was r2 = 0.95.

关键词： Feedlot Water Soluble P Bray -1 P Cattle Manure Phosphorus Runoff

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Smart biomaterials: Surfaces functionalized with proteolytically stable osteoblast-adhesive peptides

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Bioactive Materials 2017年第3期2卷 121-130页

作者： Annj zamuner Paola Brun Michele Scorzeto Giuseppe Sica Ignazio Castagliuolo Monica Dettin Department of Industrial Engineering University of PadovaVia F.Marzolo 935131PadovaItaly Department of Molecular Medicine University of PadovaVia A.Gabelli 6335121PadovaItaly Department of Biomedical Sciences University of PadovaVia U.Bassi 58/B35131PadovaItaly

Engineered scaffolds for bone tissue regeneration are designed to promote cell adhesion,growth,proliferation and ***,covalent and selective functionalization of glass and titanium surfaces with an adhesive peptide(HVP)mapped on[351e359]sequence of human Vitronectin allowed to selectively increase osteoblast attachment and adhesion strength in in vitro assays,and to promote osseointegration in in vivo *** the first time to our knowledge,in this study we investigated the resistance of adhesion sequences to proteolytic digestion:HVP was completely cleaved after 5 *** order to overcome the enzymatic degradation of the native peptide under physiological conditions we synthetized three analogues of HVP sequence.A retro-inverted peptide D-2HVP,composed of D amino acids,was completely stable in serum-containing *** addition,glass surfaces functionalized with D-2HVP increased human osteoblast adhesion as compared to the native peptide and maintained deposition of ***,D-2HVP increased expression of IBSP,VTN and SPP1 genes as compared to HVP functionalized *** internal reflection fluorescence microscope analysis showed cells with numerous filopodia spread on D-2HVP-functionalized ***,the D-2HVP sequence is proposed as new osteoblast adhesive peptide with increased bioactivity and high proteolytic resistance.

关键词： Adhesive sequences Retro-inverso peptides Surface grafting Proteolytic degradation Osteoblast TIRF

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Covalent functionalization of decellularized tissues accelerates endothelialization

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Bioactive Materials 2021年第11期6卷 3851-3864页

作者： Eleonora Dal Sasso Annj zamuner Andrea Filippi Filippo Romanato Tiziana Palmosi Luca Vedovelli Dario Gregori Jose Luís Gòmez Ribelles Teresa Russo Antonio Gloria Laura Iop Gino Gerosa Monica Dettin Department of Cardiac Thoracic and Vascular Sciences and Venetian Institute of Molecular MedicinePaduaItaly Department of Industrial Engineering University of PaduaPaduaItaly LIFELAB Program Consorzio per la Ricerca SanitariaCORISVeneto RegionItaly Department of Physics and Astronomy“G.Galilei” University of PaduaPaduaItaly Fondazione Bruno Kessler TrentoItaly Institute of Pediatric Research Citt`a della Speranza PaduaItaly Center for Biomaterials and Tissue Engineering CBITUniversitat Polit`ecnica de Val`enciaVal`enciaSpain Biomedical Research Networking Center on Bioengineering Biomaterials and Nanomedicine(CIBER-BBN)ValenciaSpain Institute of Polymers Composites and BiomaterialsNational Research Council of ItalyNaplesItaly

In the field of tissue regeneration,the lack of a stable endothelial lining may affect the hemocompatibility of both synthetic and biological *** drawbacks might be prevented by specific biomaterial functionalization to induce selective endothelial cell(EC)*** bovine pericardia and porcine aortas were selectively functionalized with a REDV tetrapeptide at 10^(-5)M and 10^(-6)M working *** scaffold-bound peptide was quantified and REDV potential EC adhesion enhancement was evaluated in vitro by static seeding of human umbilical vein *** viable cells and MTS production were statistically higher in functionalized tissues than in *** histoarchitecture,geometrical features,and mechanical properties were unaffected by peptide *** selective immobilization of REDV was effective in accelerating ECs adhesion while promoting proliferation in functionalized decellularized tissues intended for blood-contacting applications.

关键词： Decellularized pericardium Decellularized aorta Covalent functionalization Endothelialization REDV Mechanical analysis

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