Smart biomaterials: Surfaces functionalized with proteolytically stable osteoblast-adhesive peptides
作者机构：Department of Industrial EngineeringUniversity of PadovaVia F.Marzolo 935131PadovaItaly Department of Molecular MedicineUniversity of PadovaVia A.Gabelli 6335121PadovaItaly Department of Biomedical SciencesUniversity of PadovaVia U.Bassi 58/B35131PadovaItaly
出 版 物：《Bioactive Materials》 (生物活性材料（英文）)
年 卷 期：2017年第2卷第3期
页 面：121-130页
核心收录：
学科分类：081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 070303[理学-有机化学] 0703[理学-化学]
主 题：Adhesive sequences Retro-inverso peptides Surface grafting Proteolytic degradation Osteoblast TIRF
摘 要：Engineered scaffolds for bone tissue regeneration are designed to promote cell adhesion,growth,proliferation and ***,covalent and selective functionalization of glass and titanium surfaces with an adhesive peptide(HVP)mapped on[351e359]sequence of human Vitronectin allowed to selectively increase osteoblast attachment and adhesion strength in in vitro assays,and to promote osseointegration in in vivo *** the first time to our knowledge,in this study we investigated the resistance of adhesion sequences to proteolytic digestion:HVP was completely cleaved after 5 *** order to overcome the enzymatic degradation of the native peptide under physiological conditions we synthetized three analogues of HVP sequence.A retro-inverted peptide D-2HVP,composed of D amino acids,was completely stable in serum-containing *** addition,glass surfaces functionalized with D-2HVP increased human osteoblast adhesion as compared to the native peptide and maintained deposition of ***,D-2HVP increased expression of IBSP,VTN and SPP1 genes as compared to HVP functionalized *** internal reflection fluorescence microscope analysis showed cells with numerous filopodia spread on D-2HVP-functionalized ***,the D-2HVP sequence is proposed as new osteoblast adhesive peptide with increased bioactivity and high proteolytic resistance.
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