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Mitochondrial phylogeography of the Iberian endemic frog Rana iberica,with implications for its conservation

Current Zoology 2018年第6期64卷 755-764页

作者： Jose TEIXEIRA Helena GONCALVES Nuno FERRAND Mario GARCIA-PARiS ernesto recuero Centro Interdisciplinar de Investigacao Marinha e Ambiental (CIIMAR) Terminal de Cruzeiros do Porto de LeixoesMatosinhos 4450-208Portugal Centro de Investigacao em Biodiversidade e Recursos Geneticos (CIBIO-InBIO) Universidade do PortoRua Padre Armando QuintasVairao 4485-661Portugal Museu de Historia Natural e da Cieneia da Universidade do Porto (MHNC-UP) Praca Gomes TeixeiraPorto 4099-002Portugal Departamento de Biologia Faculdade de Ciencias da Universidade do PortoPorto 4099-002Portugal Museo Nacional de Ciencias Naturales (MNCN-CSIC) Calle de Jose Gutierrez Abascal2Madrid 28006Spain

Genetic characterization of species using phylogeographic approaches represents a basic reference to understand their evolutionary history as well as to identify conservation priorities to protect areas of particular interest regarding evolutionary *** in well-studied regions such information is lacking for the majority of species,including many endemic species with reduced distribution *** investigate the phylogeographic pattern of the Iberian frog Rana iberica,an endemic amphibian restricted to Central and North-Western Iberian *** mitochondrial sequences,we reconstruct the phylogeographic history of the species to test the effect of Quaternary climate changes on the evolutionary diversification of lineages,that is,the differentiation of mitochondrial lineages and the formation of genetic diversity melting pots,and integrate phylogeographic evidence for future conservation *** results indicate the existence of 3 main mitochondrial lineages differentiated during the Upper *** historical demographic analyses and climatic niche modeling show a strong effect of glacial climate changes,suggesting recurrent range contractions and *** such circumstances,differentiation took place most likely by isolation in allopatric interglacial *** lineage admixture in northern Portugal generated a broad mixed zone with highest nucleotide *** its particular evolutionary potential,its reduced distribution and eventual threats under current climate change scenario,conservation priorities should focus on the isolated lineage from Sierra de Guadalupe.

关键词： climate niche modeling conservation demography glacial refugia phylogeegraphy Amphibia

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Most soil and litter arthropods are unidentifable based on current DNA barcode reference libraries

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Current Zoology 2024年第5期70卷 637-646页

作者： ernesto recuero Frank E.Etzler Michael S.Caterino Department of Plant and Environmental Sciences Clemson University277 Poole Agricultural CenterClemsonSC 29634USA Natural Resource Section Montana Department of Agriculture302 N Roberts StHelenaMT 59601USA

We are far from knowing all species living on the *** biodiversity is demanding and requires time and *** groups are understudied given problems of identifying and delimiting *** barcoding emerged to overcome some of the difficulties in identi-fying *** limitations derive from incomplete taxonomic knowledge and the lack of comprehensive DNA barcode libraries for so many taxonomic ***,we evaluate how useful barcoding is for identifying arthropods from highly diverse leaf litter communities in the south-ern Appalachian Mountains(USA).We used 3 reference databases and several automated classification methods on a data set including several arthropod ***,Araneae,Collembola,Coleoptera,Diptera,and Hymenoptera were well represented,showing different performances across methods and *** performed the best,with correct identification rates to species and genus levels of~50%across *** performed poorly,no barcodes were identified to species or *** groups showed poor to mediocre performance,from around 3%(mites)to 20%(beetles)correctly identified barcodes to species,but also with some false *** general,BOLD-based identification offered the best identification results but,in all cases except spiders,performance is poor,with less than a fifth of specimens correctly identified to genus or *** results indicate that the soil arthropod fauna is still insufficiently documented,with many species unrepresented in DNA barcode *** effort toward integrative taxonomic characterization is needed to complete our reference libraries before we can rely on DNAbarcoding as a universally applicable identification method.

关键词： Arthropoda automated identification DNA barcoding databases taxonomy taxonomic impediment

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Limited long-distance dispersal success in a Western European fairy shrimp evidenced by nuclear and mitochondrial lineage structuring

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Current Zoology 2020年第3期66卷 227-237页

作者： Paula C.RODRIGUEZ-FLORES ernesto recuero Yolanda JIMENEZ-RUIZ Mario GARCIA-PARIS Museo Nacional de Ciencias Naturales(MNCN-CSIC) Jose Gutierrez Abascal2Madrid 28006Spain Centre d'Estudis Avanqats de Blanes(CEAB-CSIC) C.d'Acces Cala Sant Francesc 14Blanes 17300Spain

Anostraca are known by their ability for long-distance dispersal,but the existence in several species of deep,geographically structured mtDNA lineages suggests their populations are subjected to allopatric differentiation,isolation,and prevalence of local scale *** stagnalis is one of the most widespread species of Anostraca and previous studies revealed an unclear geographical pattern of mtDNA genetic ***,we analyze populations from the Iberian and Italian Peninsulas,Central Europe,and Scandinavia,with the aim to characterize the patterns of genetic diversity in a spatio-temporal framework using mtDNA and nuclear markers to test gene flow among close *** these aims we built a time-calibrated phylogeny and carried out Bayesian phylogeographic analyses using a continuous diffusion *** results indicated that *** presents a deeply structured genetic diversity,including 7 ancient lineages,some of them even predating the *** Iberian Peninsula harbors high diversity of lineages,with strong isolation and recent absence of gene flow between *** at local scale seems to be the prevailing dispersal mode of ***,which exhibits a pattern of isolation-bydistance in the Iberian *** remark the vulnerability of most of these lineages,given the limited known geographic distribution of some of them,and the high risk of losing important evolutionary potential for the species.

关键词： allopatric fragmentation Anostraca Crustacea nuclear markers Pleistocene phylogeography

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GSTM3, but not IZUMO1, is a cryotolerance marker of boar sperm

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Journal of Animal Science and Biotechnology 2019年第4期10卷 931-941页

作者： Marc Llavanera Ariadna Delgado-Bermúdez Beatriz Fernandez-Fuertes Sandra recuero Yentel Mateo Sergi Bonet Isabel Barranco Marc Yeste Biotechnology of Animal and Human Reproduction (Techno Sperm) Unit of Cell Biology Department of Biology Institute of Food and Agricultural Technology University of Girona

Background: Cryopreservation is currently the most efficient method for long-term preservation of mammalian gametes and is extensively used in swine artificial insemination(AI) centres. However, it is well-known that cryopreservation procedures induce changes in the water phase in both intra and extracellular compartments,which alter the content and localisation of several proteins and ends up curtailing the structural integrity of functional sperm(i.e., cryoinjuries). Alterations and deficiencies of sperm-oocyte binding proteins during gamete recognition are one of the causes of reproductive failure both in vitro and in vivo. In this sense, characterisation of cryopreservation effects upon oocyte-binding proteins of sperm, such as IZUMO1 and GSTM3, is essential when assessing the impact of this technique in swine ***: Cryopreservation was found to induce changes in the localisation of IZUMO1 and GSTM3 in boar ***, the relative content of both proteins was not altered after thawing. Furthermore, whereas IZUMO1 content was found not to be related to the cryotolerance of boar sperm, GSTM3 content was observed to be higher in poor(PFE) than in good(GFE) freezability ejaculates in both pre-frozen(1.00 INT·mm^2± 0.14 INT·mm^2 vs.0.72 INT·mm^2± 0.15 INT·mm^2;P < 0.05) and post-thawed(0.96 INT·mm^2± 0.20 INT·mm^2 vs. 70 INT·mm^2± 0.19 INT·mm^2;P < 0.05) samples. Moreover, GSTM3 levels were found to be higher in those spermatozoa that exhibited low mitochondrial activity, high reactive oxygen species(ROS) production, and high membrane lipid disorder postthaw(P < 0.05).Conclusions: The difference in GSTM3 content between GFE and PFE, together with this protein having been found to be related to poor sperm quality post-thaw, suggests that it could be used as a cryotolerance marker of boar spermatozoa. Furthermore, both IZUMO1 and GSTM3 relocate during cryopreservation, which could contribute to the reduced fertilising capacity of frozen-thawed boar

关键词： Boar Cryopreservation GSTM3 IZUMO1 ROS Sperm

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Sperm chromatin condensation as an in vivo fertility biomarker in bulls:a flow cytometry approach

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Journal of Animal Science and Biotechnology 2022年第2期13卷 418-429页

作者： Marc Llavanera Jordi Ribas-Maynou Ariadna Delgado-Bermúdez Sandra recuero Rodrigo Muiño Carlos OHidalgo Carolina Tamargo Sergi Bonet Yentel Mateo-Otero Marc Yeste Biotechnology of Animal and Human Reproduction(TechnoSperm) Faculty of SciencesUniversity of GironaC/Maria Aurèlia Campany69ES-17003 GironaSpain Department of Biology Unit of Cell BiologyFaculty of SciencesUniversity of GironaES-17003 GironaSpain Department of Animal Pathology Faculty of Veterinary MedicineUniversity of Santiago de CompostelaES-15705 LugoSpain Department of Animal Selection and Reproduction The Regional Agri-Food Research and Development Service of Asturias(SERIDA)E-33394 GijónSpain

Background:Genetic selection in cattle has been directed to increase milk ***,coupled to the fact that the vast majority of bovine artificial inseminations(AI)are performed using cryopreserved sperm,have led to a reduction of fertility rates over the ***,seeking sensitive and specific sperm biomarkers able to predict fertility rates is of vital importance to improve cattle reproductive *** humans,sperm chromatin condensation evaluated through chromomycin A3(CMA3)has recently been purported to be a powerful biomarker for sperm functional status and male *** objectives of the present study were:a)to set up a flow cytometry method for simultaneously evaluating chromatin condensation and sperm viability,and b)to test whether this parameter could be used as a predictor of in vivo fertility in *** study included pools of three independent cryopreserved ejaculates per bull from 25 Holstein *** outcomes of each sire were determined by non-return rates,which were used to classify bulls into two groups(highly fertile and subfertile).Results:Chromatin condensation status of bovine sperm was evaluated through the combination of CMA3 and Yo-Pro-1 staining and flow *** quality parameters(morphology,viability,total and progressive motility)were also *** correlation coefficients and ROC curves were calculated to assess their capacity to predict in vivo *** morphology,viability and total motility presented an area under the ROC curve(AUC)of 0.54,0.64 and 0.68,respectively(P>0.05),and thus were not able to discriminate between fertile and subfertile ***,while the percentage of progressively motile sperm showed a significant predictive value,with an AUC of 0.73(P=0.05),CMA3/Yo-Pro-1 staining even depicted superior results for the prediction of in vivo fertility in ***,the percentage of viable sperm with poor chromatin condensation showed better accura

关键词： Bull Chromatin Chromomycin A3 Condensation Fertility Flow cytometry Sperm

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Aquaglyceroporins but not orthodox aquaporins are involved in the cryotolerance of pig spermatozoa

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Journal of Animal Science and Biotechnology 2020年第1期11卷 20-31页

作者： Ariadna Delgado-Bermúdez Marc Llavanera Leira Fernández-Bastit Sandra recuero Yentel Mateo-Otero Sergi Bonet Isabel Barranco Beatriz Fernández-Fuertes Marc Yeste Biotechnology of Animal and Human Reproduction(TechnoSperm) Unit of Cell BiologyDepartment of BiologyFaculty of SciencesInstitute of Food and Agricultural TechnologyUniversity of GironaC/Maria Aurèlia Campany69Campus MontiliviE-17003 GironaSpain

Background:Aquaporins(AQPs)are a family of transmembrane water channels that includes orthodox AQPs,aquaglyceroporins(GLPs)and super ***3,AQP7,AQP9 and AQP11 have been identified in boar sperm,and they are crucial for sperm maturation and *** exchange is an important event in cryopreservation,which is the most efficient method for long-term storage of ***,the freezethaw process leads to sperm damage and a loss of fertilizing *** that the quality of frozenthawed sperm partially depends on the regulation of osmolality variations during this process,AQPs might play a crucial role in boar semen *** this context,the aim of this study was to unravel the functional relevance of the different groups of AQPs for boar sperm cryotolerance through three different ***:Inhibition of different groups of AQPs was found to have different effects on boar sperm *** the use of 1,3-propanediol(PDO),an inhibitor of orthodox AQPs and GLPs,decreased total motility(P<0.05),it increased post-thaw sperm viability,lowered membrane lipid disorder and increased mitochondrial membrane potential(MMP)(P<0.05).When acetazolamide(AC)was used as an inhibitor of orthodox AQPs,the effects on post-thaw sperm quality were restricted to a mild increase in MMP in the presence of the intermediate concentration at 30 min post-thaw and an increase in superoxide levels(P<0.05).Finally,the addition of phloretin(PHL),a GLP inhibitor,had detrimental effects on post-thaw total and progressive sperm motilities,viability and lipid membrane disorder(P<0.05).Conclusions:The effects of the different inhibitors suggest that GLPs rather than orthodox AQPs are relevant for boar sperm ***,the positive effect of PDO on sperm quality suggests a cryoprotective role for this molecule.

关键词： Acetazolamide Aquaporins Boar Phloretin Propanediol Sperm

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Simulation, Construction and Characterization of a Piezoelectric Transducer Using Rexolite as Acoustic Matching

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材料科学与工程（中英文B版） 2013年第4期3卷 208-213页

作者： Israel Sanchez Dominguez Pedro Acevedo Contla Manuel recuero Lopez Demetrio Fabian Garcia Nocetti Polytechnic University of Madrid School of Industrial Engineering Madrid Spain National Autonomous University of Mexico lnstitutito Research in Applied Mathematics and Systems Mexico City Mexico

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Prevalence of hepatocarcinoma-related hepatitis B virus mutants in patients in grey zone of treatment

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World Journal of Gastroenterology 2019年第38期25卷 5883-5896页

作者： Ana Isabel Gil-García Antonio Madejón Irene Francisco-recuero Ana López-López Emiliana Villafranca Miriam Romero Araceli García Antonio Olveira Rocío Mena Juan Ramón Larrubia Javier García-Samaniego Hepatology Unit Hospital Universitario La PazMadrid 28046Spain Centro de Investigación Biomédica en Red Instituto de Salud Carlos IIIMadrid 28029Spain Instituto de Investigación del Hospital Universitario la Paz Madrid 28046Spain Biochemistry Department Faculty of MedicineUniversidad Autónoma de MadridMadrid 28029Spain Instituto de Genética Médica y Molecular Hospital Universitario La PazMadrid 28046Spain Translational Hepatology Unit Hospital General Universitario de GuadalajaraGuadalajara 19002Castilla-La ManchaSpain

BACKGROUND Antiviral treatment of patients with chronic hepatitis B(CHB)in the grey zone of treatment comands risk management in order to optimize the health *** this sense,the identification of HBV mutants related with an increased risk of hepatocellular carcinoma(HCC)could be useful to identify subpopulations with potential indication of antiviral *** To analyze the prevalence/persistence of hepatitis B virus(HBV)preS and basal core promoter(BCP)/precore/core variants associated to HCC development in CHB patients in the grey *** Work was designed as a longitudinal retrospective study,including 106 plasma samples from 31 patients with CHB in the grey zone of treatment:Hepatitis B e antigen negative,HBV-DNA levels between 12-20000 IU/mL,normal or discordant transaminase levels during follow up and mild/moderate necroinflammatory activity in liver biopsy or Fibroscan(up to 9.5 kPa).Serum HBVDNA was tested using the Abbott Real Time HBV Assay and the BCP/precore/core and the hepatitis B surface antigen(HBsAg)coding regions were analyzed in positive samples by PCR/bulk-sequencing to identify the HCCrelated HBV *** High-risk HCC related mutants were detected in 24(77%)patients:19(61%)in the BCP/precore/core,and 7(23%)in the HBsAg coding region(2 preS1 and 5 preS2 deletions).The prevalence of preS deletions was genotype-dependent:3/5(60%)patients with preS2 deletions and 1/2 with preS1 deletions were infected with the HBV-E *** HBV-E was the most prevalent in sub-Saharan patients,a correlation between preS deletions and ethnicity was also found:6/8(75%)sub-Saharan vs 1/19(5%)Caucasian patients had preS deletions(P=0.00016).Remarkably,this correlation was maintained in those patients infected with HBV-A,a minor genotype in sub-Saharan patients:2/2 patients infected with HBV-A from West Africa vs 0/6 of Caucasian origin had preS *** HCC related variants were the major strains and persisted over time(up to 48 mo).Pa

关键词： Hepatitis B virus Hepatocellular carcinoma PreS deletions Hepatitis B virus treatment Grey zone

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西班牙众议院大厦里的语言清晰度

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电声技术 2010年第9期34卷 4-9页

作者： C.ASENSIO R.PAGAN J.A.TRUJILLO M.recuero 王富裕 Universidad Politécnica de Madrid Grupo de Investigación en Instrumentación y Acústica Aplicada(I2A2)Madrid 28031Spain 飞利浦电子深圳研发中心

描述了在马德里的西班牙众议院会议厅半圆形建筑中检测到的声学问题,给出了解决该问题的步骤和流程。为了量化混响时间、快速语言传输指数(RASTI)及礼堂内辅助系统产生背景噪声级的声学特征,在半圆形建筑空场中做了一些声学测量。建立... 详细信息

描述了在马德里的西班牙众议院会议厅半圆形建筑中检测到的声学问题,给出了解决该问题的步骤和流程。为了量化混响时间、快速语言传输指数(RASTI)及礼堂内辅助系统产生背景噪声级的声学特征,在半圆形建筑空场中做了一些声学测量。建立了一个仿真模型并通过在空场条件中的测量进行了验证,当开会期间只允许进行声压级测量情况下,可以用该模型来模拟半圆形建筑在开会期间的声学指标。考虑了多种不同方面来优化会议期间的语言清晰度,提出和模拟过的建议主要包括降低会议期间的背景噪声级和提升扩声系统。

关键词：语言清晰度混响时间快速语言传输指数

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非典型表现的神经纤维瘤病

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世界核心医学期刊文摘（皮肤病学分册） 2006年第3期2卷 16-16页

作者： Tato B.P. Sáez A.C. recuero J.L.D. 吴佳纹 C/Suero de Quiones 4 Madrid 28002 Spain

Neurofibromatosis (NF) is considered to be a heterogeneous neuroectodermal disease clinically denned by the presence of neurofibromas, multiple caf′ e-au-lait spots, intertriginous freckles and Lisch nodules. Mosaicism explains atypical presentations of the disease. Early mutations, before tissue differentiation, give rise to generalized disease. We report an atypical presentation of neurofibromatosis with an unusual distribution of neurofibromas, a peculiar, clinically and pathologically, neurofibroma on the trunk and the association with an ovarian serous cystoadenofibroma.

关键词：神经纤维瘤病非典型表现囊性腺纤维瘤临床表现神经外胚层卵巢浆液性嵌合现象组织分化咖啡斑多发性

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